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Q969K3

- RNF34_HUMAN

UniProt

Q969K3 - RNF34_HUMAN

Protein

E3 ubiquitin-protein ligase RNF34

Gene

RNF34

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate.3 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei232 – 2332Cleavage; by caspase-3

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri56 – 10752FYVE-typeAdd
    BLAST
    Zinc fingeri325 – 36036RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. phosphatidylinositol phosphate binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. ubiquitin-protein transferase activity Source: UniProtKB
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: UniProtKB
    2. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
    3. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Apoptosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase RNF34 (EC:6.3.2.-)
    Alternative name(s):
    Caspase regulator CARP1
    Caspases-8 and -10-associated RING finger protein 1
    Short name:
    CARP-1
    FYVE-RING finger protein Momo
    Human RING finger homologous to inhibitor of apoptosis protein
    Short name:
    hRFI
    RING finger protein 34
    RING finger protein RIFF
    Gene namesi
    Name:RNF34
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:17297. RNF34.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. endomembrane system Source: UniProtKB-SubCell
    3. nuclear speck Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi342 – 3421H → A: Loss of E3 ubiquitin protein ligase activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA34436.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372E3 ubiquitin-protein ligase RNF34PRO_0000056072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei254 – 2541Phosphoserine2 Publications

    Post-translational modificationi

    Auto-ubiquitinated (in vitro).By similarity
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ969K3.
    PaxDbiQ969K3.
    PRIDEiQ969K3.

    PTM databases

    PhosphoSiteiQ969K3.

    Expressioni

    Tissue specificityi

    Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.2 Publications

    Gene expression databases

    ArrayExpressiQ969K3.
    BgeeiQ969K3.
    CleanExiHS_RNF34.
    GenevestigatoriQ969K3.

    Interactioni

    Subunit structurei

    Interacts with DNAJA3 Potential. Interacts with CASP8 and CASP10.2 PublicationsCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CASP8Q147903EBI-2340642,EBI-78060

    Protein-protein interaction databases

    BioGridi123169. 16 interactions.
    IntActiQ969K3. 6 interactions.
    STRINGi9606.ENSP00000346850.

    Structurei

    3D structure databases

    ProteinModelPortaliQ969K3.
    SMRiQ969K3. Positions 62-148, 315-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini115 – 13420SAP 1Add
    BLAST
    Domaini264 – 27815SAP 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi223 – 23412Asp-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FYVE-type zinc finger.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 2 SAP domains.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri56 – 10752FYVE-typeAdd
    BLAST
    Zinc fingeri325 – 36036RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG121302.
    HOGENOMiHOG000068080.
    HOVERGENiHBG055079.
    PhylomeDBiQ969K3.
    TreeFamiTF325195.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR011011. Znf_FYVE_PHD.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF57903. SSF57903. 1 hit.
    PROSITEiPS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q969K3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKAGATSMWA SCCGLLNEVM GTGAVRGQQS AFAGATGPFR FTPNPEFSTY    50
    PPAATEGPNI VCKACGLSFS VFRKKHVCCD CKKDFCSVCS VLQENLRRCS 100
    TCHLLQETAF QRPQLMRLKV KDLRQYLILR NIPIDTCREK EDLVDLVLCH 150
    HGLGSEDDMD TSSLNSSRSQ TSSFFTRSFF SNYTAPSATM SSFQGELMDG 200
    DQTSRSGVPA QVQSEITSAN TEDDDDDDDE DDDDEEENAE DRNPGLSKER 250
    VRASLSDLSS LDDVEGMSVR QLKEILARNF VNYSGCCEKW ELVEKVNRLY 300
    KENEENQKSY GERLQLQDEE DDSLCRICMD AVIDCVLLEC GHMVTCTKCG 350
    KRMSECPICR QYVVRAVHVF KS 372
    Length:372
    Mass (Da):41,641
    Last modified:December 1, 2001 - v1
    Checksum:iC044B83BD8591FFA
    GO
    Isoform 2 (identifier: Q969K3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MR

    Show »
    Length:373
    Mass (Da):41,797
    Checksum:i0177B2F0FACD62FF
    GO

    Sequence cautioni

    The sequence BAH14169.1 differs from that shown. Reason: Intron retention.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti183 – 1831Y → C in BAB15132. (PubMed:14702039)Curated
    Sequence conflicti238 – 2381N → D in BAB15132. (PubMed:14702039)Curated
    Sequence conflicti333 – 3331I → V in BAB15132. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MR in isoform 2. 2 PublicationsVSP_038341

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB084914 mRNA. Translation: BAC11802.1.
    AF306709 mRNA. Translation: AAK51328.1.
    AY098934 mRNA. Translation: AAM29180.1.
    AK304366 mRNA. Translation: BAH14169.1. Sequence problems.
    AK025439 mRNA. Translation: BAB15132.1.
    BT007283 mRNA. Translation: AAP35947.1.
    CR457342 mRNA. Translation: CAG33623.1.
    BC007826 mRNA. Translation: AAH07826.1.
    CCDSiCCDS31915.1. [Q969K3-1]
    CCDS9221.1. [Q969K3-2]
    RefSeqiNP_001243787.1. NM_001256858.1.
    NP_079402.2. NM_025126.3. [Q969K3-1]
    NP_919247.1. NM_194271.2. [Q969K3-2]
    UniGeneiHs.292804.

    Genome annotation databases

    EnsembliENST00000361234; ENSP00000355137; ENSG00000170633. [Q969K3-1]
    ENST00000392465; ENSP00000376258; ENSG00000170633. [Q969K3-2]
    GeneIDi80196.
    KEGGihsa:80196.
    UCSCiuc001uak.2. human. [Q969K3-2]
    uc001ual.2. human. [Q969K3-1]

    Polymorphism databases

    DMDMi74760679.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB084914 mRNA. Translation: BAC11802.1 .
    AF306709 mRNA. Translation: AAK51328.1 .
    AY098934 mRNA. Translation: AAM29180.1 .
    AK304366 mRNA. Translation: BAH14169.1 . Sequence problems.
    AK025439 mRNA. Translation: BAB15132.1 .
    BT007283 mRNA. Translation: AAP35947.1 .
    CR457342 mRNA. Translation: CAG33623.1 .
    BC007826 mRNA. Translation: AAH07826.1 .
    CCDSi CCDS31915.1. [Q969K3-1 ]
    CCDS9221.1. [Q969K3-2 ]
    RefSeqi NP_001243787.1. NM_001256858.1.
    NP_079402.2. NM_025126.3. [Q969K3-1 ]
    NP_919247.1. NM_194271.2. [Q969K3-2 ]
    UniGenei Hs.292804.

    3D structure databases

    ProteinModelPortali Q969K3.
    SMRi Q969K3. Positions 62-148, 315-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123169. 16 interactions.
    IntActi Q969K3. 6 interactions.
    STRINGi 9606.ENSP00000346850.

    PTM databases

    PhosphoSitei Q969K3.

    Polymorphism databases

    DMDMi 74760679.

    Proteomic databases

    MaxQBi Q969K3.
    PaxDbi Q969K3.
    PRIDEi Q969K3.

    Protocols and materials databases

    DNASUi 80196.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361234 ; ENSP00000355137 ; ENSG00000170633 . [Q969K3-1 ]
    ENST00000392465 ; ENSP00000376258 ; ENSG00000170633 . [Q969K3-2 ]
    GeneIDi 80196.
    KEGGi hsa:80196.
    UCSCi uc001uak.2. human. [Q969K3-2 ]
    uc001ual.2. human. [Q969K3-1 ]

    Organism-specific databases

    CTDi 80196.
    GeneCardsi GC12P121837.
    HGNCi HGNC:17297. RNF34.
    MIMi 608299. gene.
    neXtProti NX_Q969K3.
    PharmGKBi PA34436.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG121302.
    HOGENOMi HOG000068080.
    HOVERGENi HBG055079.
    PhylomeDBi Q969K3.
    TreeFami TF325195.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    GeneWikii RNF34.
    GenomeRNAii 80196.
    NextBioi 70550.
    PROi Q969K3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q969K3.
    Bgeei Q969K3.
    CleanExi HS_RNF34.
    Genevestigatori Q969K3.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR011011. Znf_FYVE_PHD.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57903. SSF57903. 1 hit.
    PROSITEi PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer."
      Sasaki S., Nakamura T., Arakawa H., Mori M., Watanabe T., Nagawa H., Croce C.M.
      Oncogene 21:5024-5030(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DNAJA3, CLEAVAGE BY CASPASE-3, TISSUE SPECIFICITY.
    2. "Cloning of RIFF, a novel RING finger FYVE finger protein expressed in human fetal brain."
      Olsson P.-A., Lindholm D.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. Kanbe D., Araki K., Nawa H.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Trachea.
    5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Kidney.
    8. "Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth."
      McDonald E.R. III, El-Deiry W.S.
      Proc. Natl. Acad. Sci. U.S.A. 101:6170-6175(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CASP8 AND CASP10, MUTAGENESIS OF HIS-342, PROTEOLYTIC DEGRADATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    9. "Overexpression of hRFI (human RING finger homologous to inhibitor of apoptosis protein type) inhibits death receptor-mediated apoptosis in colorectal cancer cells."
      Konishi T., Sasaki S., Watanabe T., Kitayama J., Nagawa H.
      Mol. Cancer Ther. 4:743-750(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiRNF34_HUMAN
    AccessioniPrimary (citable) accession number: Q969K3
    Secondary accession number(s): B7Z933, Q8NG47, Q9H6W8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Was isolated as potential interaction partner of the mitochondrial protein DNAJA3, but the 2 proteins are not in the same cellular compartments.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3