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Q969K3 (RNF34_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase RNF34

EC=6.3.2.-
Alternative name(s):
Caspase regulator CARP1
Caspases-8 and -10-associated RING finger protein 1
Short name=CARP-1
FYVE-RING finger protein Momo
Human RING finger homologous to inhibitor of apoptosis protein
Short name=hRFI
RING finger protein 34
RING finger protein RIFF
Gene names
Name:RNF34
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate. Ref.1 Ref.8 Ref.9

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with DNAJA3 Potential. Interacts with CASP8 and CASP10. Ref.1 Ref.8

Subcellular location

Nucleus speckle. Endomembrane system; Peripheral membrane protein Ref.1 Ref.8.

Tissue specificity

Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Ref.1 Ref.8

Post-translational modification

Auto-ubiquitinated (in vitro) By similarity.

Proteolytically cleaved by caspases upon induction of apoptosis by TNF. Ref.1

Sequence similarities

Contains 1 FYVE-type zinc finger.

Contains 1 RING-type zinc finger.

Contains 2 SAP domains.

Caution

Was isolated as potential interaction partner of the mitochondrial protein DNAJA3, but the 2 proteins are not in the same cellular compartments.

Sequence caution

The sequence BAH14169.1 differs from that shown. Reason: Intron retention.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CASP8Q147903EBI-2340642,EBI-78060

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q969K3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q969K3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372E3 ubiquitin-protein ligase RNF34
PRO_0000056072

Regions

Domain115 – 13420SAP 1
Domain264 – 27815SAP 2
Zinc finger56 – 10752FYVE-type
Zinc finger325 – 36036RING-type
Compositional bias223 – 23412Asp-rich

Sites

Site232 – 2332Cleavage; by caspase-3

Amino acid modifications

Modified residue2541Phosphoserine Ref.10 Ref.11

Natural variations

Alternative sequence11M → MR in isoform 2.
VSP_038341

Experimental info

Mutagenesis3421H → A: Loss of E3 ubiquitin protein ligase activity. Ref.8
Sequence conflict1831Y → C in BAB15132. Ref.4
Sequence conflict2381N → D in BAB15132. Ref.4
Sequence conflict3331I → V in BAB15132. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: C044B83BD8591FFA

FASTA37241,641
        10         20         30         40         50         60 
MKAGATSMWA SCCGLLNEVM GTGAVRGQQS AFAGATGPFR FTPNPEFSTY PPAATEGPNI 

        70         80         90        100        110        120 
VCKACGLSFS VFRKKHVCCD CKKDFCSVCS VLQENLRRCS TCHLLQETAF QRPQLMRLKV 

       130        140        150        160        170        180 
KDLRQYLILR NIPIDTCREK EDLVDLVLCH HGLGSEDDMD TSSLNSSRSQ TSSFFTRSFF 

       190        200        210        220        230        240 
SNYTAPSATM SSFQGELMDG DQTSRSGVPA QVQSEITSAN TEDDDDDDDE DDDDEEENAE 

       250        260        270        280        290        300 
DRNPGLSKER VRASLSDLSS LDDVEGMSVR QLKEILARNF VNYSGCCEKW ELVEKVNRLY 

       310        320        330        340        350        360 
KENEENQKSY GERLQLQDEE DDSLCRICMD AVIDCVLLEC GHMVTCTKCG KRMSECPICR 

       370 
QYVVRAVHVF KS 

« Hide

Isoform 2 [UniParc].

Checksum: 0177B2F0FACD62FF
Show »

FASTA37341,797

References

« Hide 'large scale' references
[1]"Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer."
Sasaki S., Nakamura T., Arakawa H., Mori M., Watanabe T., Nagawa H., Croce C.M.
Oncogene 21:5024-5030(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DNAJA3, CLEAVAGE BY CASPASE-3, TISSUE SPECIFICITY.
[2]"Cloning of RIFF, a novel RING finger FYVE finger protein expressed in human fetal brain."
Olsson P.-A., Lindholm D.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]Kanbe D., Araki K., Nawa H.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Trachea.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
[8]"Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth."
McDonald E.R. III, El-Deiry W.S.
Proc. Natl. Acad. Sci. U.S.A. 101:6170-6175(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CASP8 AND CASP10, MUTAGENESIS OF HIS-342, PROTEOLYTIC DEGRADATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]"Overexpression of hRFI (human RING finger homologous to inhibitor of apoptosis protein type) inhibits death receptor-mediated apoptosis in colorectal cancer cells."
Konishi T., Sasaki S., Watanabe T., Kitayama J., Nagawa H.
Mol. Cancer Ther. 4:743-750(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB084914 mRNA. Translation: BAC11802.1.
AF306709 mRNA. Translation: AAK51328.1.
AY098934 mRNA. Translation: AAM29180.1.
AK304366 mRNA. Translation: BAH14169.1. Sequence problems.
AK025439 mRNA. Translation: BAB15132.1.
BT007283 mRNA. Translation: AAP35947.1.
CR457342 mRNA. Translation: CAG33623.1.
BC007826 mRNA. Translation: AAH07826.1.
RefSeqNP_001243787.1. NM_001256858.1.
NP_079402.2. NM_025126.3.
NP_919247.1. NM_194271.2.
UniGeneHs.292804.

3D structure databases

ProteinModelPortalQ969K3.
SMRQ969K3. Positions 62-148, 315-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123169. 16 interactions.
IntActQ969K3. 6 interactions.
STRING9606.ENSP00000346850.

PTM databases

PhosphoSiteQ969K3.

Polymorphism databases

DMDM74760679.

Proteomic databases

PaxDbQ969K3.
PRIDEQ969K3.

Protocols and materials databases

DNASU80196.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361234; ENSP00000355137; ENSG00000170633. [Q969K3-1]
ENST00000392465; ENSP00000376258; ENSG00000170633. [Q969K3-2]
ENST00000606095; ENSP00000476071; ENSG00000271829. [Q969K3-1]
ENST00000606383; ENSP00000476203; ENSG00000271829. [Q969K3-2]
GeneID80196.
KEGGhsa:80196.
UCSCuc001uak.2. human. [Q969K3-2]
uc001ual.2. human. [Q969K3-1]

Organism-specific databases

CTD80196.
GeneCardsGC12P121837.
HGNCHGNC:17297. RNF34.
MIM608299. gene.
neXtProtNX_Q969K3.
PharmGKBPA34436.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG121302.
HOGENOMHOG000068080.
HOVERGENHBG055079.
PhylomeDBQ969K3.
TreeFamTF325195.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ969K3.
BgeeQ969K3.
CleanExHS_RNF34.
GenevestigatorQ969K3.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF57903. SSF57903. 1 hit.
PROSITEPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiRNF34.
GenomeRNAi80196.
NextBio70550.
PROQ969K3.
SOURCESearch...

Entry information

Entry nameRNF34_HUMAN
AccessionPrimary (citable) accession number: Q969K3
Secondary accession number(s): B7Z933, Q8NG47, Q9H6W8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM