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Q969K3

- RNF34_HUMAN

UniProt

Q969K3 - RNF34_HUMAN

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Protein

E3 ubiquitin-protein ligase RNF34

Gene
RNF34
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate.3 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei232 – 2332Cleavage; by caspase-3

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri56 – 10752FYVE-typeAdd
BLAST
Zinc fingeri325 – 36036RING-typeAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. phosphatidylinositol phosphate binding Source: UniProtKB
  3. protein binding Source: IntAct
  4. ubiquitin-protein transferase activity Source: UniProtKB
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: UniProtKB
  2. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  3. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  4. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF34 (EC:6.3.2.-)
Alternative name(s):
Caspase regulator CARP1
Caspases-8 and -10-associated RING finger protein 1
Short name:
CARP-1
FYVE-RING finger protein Momo
Human RING finger homologous to inhibitor of apoptosis protein
Short name:
hRFI
RING finger protein 34
RING finger protein RIFF
Gene namesi
Name:RNF34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:17297. RNF34.

Subcellular locationi

Nucleus speckle. Endomembrane system; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endomembrane system Source: UniProtKB-SubCell
  3. nuclear speck Source: UniProtKB-SubCell
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi342 – 3421H → A: Loss of E3 ubiquitin protein ligase activity. 1 Publication

Organism-specific databases

PharmGKBiPA34436.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372E3 ubiquitin-protein ligase RNF34PRO_0000056072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei254 – 2541Phosphoserine2 Publications

Post-translational modificationi

Auto-ubiquitinated (in vitro) By similarity.
Proteolytically cleaved by caspases upon induction of apoptosis by TNF.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ969K3.
PaxDbiQ969K3.
PRIDEiQ969K3.

PTM databases

PhosphoSiteiQ969K3.

Expressioni

Tissue specificityi

Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.2 Publications

Gene expression databases

ArrayExpressiQ969K3.
BgeeiQ969K3.
CleanExiHS_RNF34.
GenevestigatoriQ969K3.

Interactioni

Subunit structurei

Interacts with DNAJA3 Reviewed prediction. Interacts with CASP8 and CASP10.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CASP8Q147903EBI-2340642,EBI-78060

Protein-protein interaction databases

BioGridi123169. 16 interactions.
IntActiQ969K3. 6 interactions.
STRINGi9606.ENSP00000346850.

Structurei

3D structure databases

ProteinModelPortaliQ969K3.
SMRiQ969K3. Positions 62-148, 315-359.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 13420SAP 1Add
BLAST
Domaini264 – 27815SAP 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi223 – 23412Asp-richAdd
BLAST

Sequence similaritiesi

Contains 2 SAP domains.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri56 – 10752FYVE-typeAdd
BLAST
Zinc fingeri325 – 36036RING-typeAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG121302.
HOGENOMiHOG000068080.
HOVERGENiHBG055079.
PhylomeDBiQ969K3.
TreeFamiTF325195.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q969K3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKAGATSMWA SCCGLLNEVM GTGAVRGQQS AFAGATGPFR FTPNPEFSTY    50
PPAATEGPNI VCKACGLSFS VFRKKHVCCD CKKDFCSVCS VLQENLRRCS 100
TCHLLQETAF QRPQLMRLKV KDLRQYLILR NIPIDTCREK EDLVDLVLCH 150
HGLGSEDDMD TSSLNSSRSQ TSSFFTRSFF SNYTAPSATM SSFQGELMDG 200
DQTSRSGVPA QVQSEITSAN TEDDDDDDDE DDDDEEENAE DRNPGLSKER 250
VRASLSDLSS LDDVEGMSVR QLKEILARNF VNYSGCCEKW ELVEKVNRLY 300
KENEENQKSY GERLQLQDEE DDSLCRICMD AVIDCVLLEC GHMVTCTKCG 350
KRMSECPICR QYVVRAVHVF KS 372
Length:372
Mass (Da):41,641
Last modified:December 1, 2001 - v1
Checksum:iC044B83BD8591FFA
GO
Isoform 2 (identifier: Q969K3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MR

Show »
Length:373
Mass (Da):41,797
Checksum:i0177B2F0FACD62FF
GO

Sequence cautioni

The sequence BAH14169.1 differs from that shown. Reason: Intron retention.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MR in isoform 2. VSP_038341

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831Y → C in BAB15132. 1 Publication
Sequence conflicti238 – 2381N → D in BAB15132. 1 Publication
Sequence conflicti333 – 3331I → V in BAB15132. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB084914 mRNA. Translation: BAC11802.1.
AF306709 mRNA. Translation: AAK51328.1.
AY098934 mRNA. Translation: AAM29180.1.
AK304366 mRNA. Translation: BAH14169.1. Sequence problems.
AK025439 mRNA. Translation: BAB15132.1.
BT007283 mRNA. Translation: AAP35947.1.
CR457342 mRNA. Translation: CAG33623.1.
BC007826 mRNA. Translation: AAH07826.1.
CCDSiCCDS31915.1. [Q969K3-1]
CCDS9221.1. [Q969K3-2]
RefSeqiNP_001243787.1. NM_001256858.1.
NP_079402.2. NM_025126.3. [Q969K3-1]
NP_919247.1. NM_194271.2. [Q969K3-2]
UniGeneiHs.292804.

Genome annotation databases

EnsembliENST00000361234; ENSP00000355137; ENSG00000170633. [Q969K3-1]
ENST00000392465; ENSP00000376258; ENSG00000170633. [Q969K3-2]
ENST00000606095; ENSP00000476071; ENSG00000271829. [Q969K3-1]
ENST00000606383; ENSP00000476203; ENSG00000271829. [Q969K3-2]
GeneIDi80196.
KEGGihsa:80196.
UCSCiuc001uak.2. human. [Q969K3-2]
uc001ual.2. human. [Q969K3-1]

Polymorphism databases

DMDMi74760679.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB084914 mRNA. Translation: BAC11802.1 .
AF306709 mRNA. Translation: AAK51328.1 .
AY098934 mRNA. Translation: AAM29180.1 .
AK304366 mRNA. Translation: BAH14169.1 . Sequence problems.
AK025439 mRNA. Translation: BAB15132.1 .
BT007283 mRNA. Translation: AAP35947.1 .
CR457342 mRNA. Translation: CAG33623.1 .
BC007826 mRNA. Translation: AAH07826.1 .
CCDSi CCDS31915.1. [Q969K3-1 ]
CCDS9221.1. [Q969K3-2 ]
RefSeqi NP_001243787.1. NM_001256858.1.
NP_079402.2. NM_025126.3. [Q969K3-1 ]
NP_919247.1. NM_194271.2. [Q969K3-2 ]
UniGenei Hs.292804.

3D structure databases

ProteinModelPortali Q969K3.
SMRi Q969K3. Positions 62-148, 315-359.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123169. 16 interactions.
IntActi Q969K3. 6 interactions.
STRINGi 9606.ENSP00000346850.

PTM databases

PhosphoSitei Q969K3.

Polymorphism databases

DMDMi 74760679.

Proteomic databases

MaxQBi Q969K3.
PaxDbi Q969K3.
PRIDEi Q969K3.

Protocols and materials databases

DNASUi 80196.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000361234 ; ENSP00000355137 ; ENSG00000170633 . [Q969K3-1 ]
ENST00000392465 ; ENSP00000376258 ; ENSG00000170633 . [Q969K3-2 ]
ENST00000606095 ; ENSP00000476071 ; ENSG00000271829 . [Q969K3-1 ]
ENST00000606383 ; ENSP00000476203 ; ENSG00000271829 . [Q969K3-2 ]
GeneIDi 80196.
KEGGi hsa:80196.
UCSCi uc001uak.2. human. [Q969K3-2 ]
uc001ual.2. human. [Q969K3-1 ]

Organism-specific databases

CTDi 80196.
GeneCardsi GC12P121837.
HGNCi HGNC:17297. RNF34.
MIMi 608299. gene.
neXtProti NX_Q969K3.
PharmGKBi PA34436.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG121302.
HOGENOMi HOG000068080.
HOVERGENi HBG055079.
PhylomeDBi Q969K3.
TreeFami TF325195.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GeneWikii RNF34.
GenomeRNAii 80196.
NextBioi 70550.
PROi Q969K3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q969K3.
Bgeei Q969K3.
CleanExi HS_RNF34.
Genevestigatori Q969K3.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
SUPFAMi SSF57903. SSF57903. 1 hit.
PROSITEi PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer."
    Sasaki S., Nakamura T., Arakawa H., Mori M., Watanabe T., Nagawa H., Croce C.M.
    Oncogene 21:5024-5030(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DNAJA3, CLEAVAGE BY CASPASE-3, TISSUE SPECIFICITY.
  2. "Cloning of RIFF, a novel RING finger FYVE finger protein expressed in human fetal brain."
    Olsson P.-A., Lindholm D.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. Kanbe D., Araki K., Nawa H.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Trachea.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  8. "Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth."
    McDonald E.R. III, El-Deiry W.S.
    Proc. Natl. Acad. Sci. U.S.A. 101:6170-6175(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CASP8 AND CASP10, MUTAGENESIS OF HIS-342, PROTEOLYTIC DEGRADATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  9. "Overexpression of hRFI (human RING finger homologous to inhibitor of apoptosis protein type) inhibits death receptor-mediated apoptosis in colorectal cancer cells."
    Konishi T., Sasaki S., Watanabe T., Kitayama J., Nagawa H.
    Mol. Cancer Ther. 4:743-750(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiRNF34_HUMAN
AccessioniPrimary (citable) accession number: Q969K3
Secondary accession number(s): B7Z933, Q8NG47, Q9H6W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was isolated as potential interaction partner of the mitochondrial protein DNAJA3, but the 2 proteins are not in the same cellular compartments.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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