Reviewed, UniProtKB/Swiss-Prot Q969H6 (POP5_HUMAN)

Names and origin

Protein names

Recommended name:
    Ribonuclease P/MRP protein subunit POP5
      Short name=hPop5
    EC=3.1.26.5

Gene names

Name:	POP5
ORF Names:	AD-008, HSPC004, x0003

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. Ref.1
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. Ref.1
Subunit structure	Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a RNA moiety and at least 8 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38 and RPP40. RNase MRP consists of a RNA moiety and at least 9 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38, RPP40, POP5 and RPP21. Ref.1
Subcellular location	Nucleus › nucleolus.
Miscellaneous	The last C-terminal 19 amino acids are not required for complex association and RNase activity.
Sequence similarities	Belongs to the eukaryotic/archaeal RNase P protein component 2 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.6 Inferred from physical interaction. Source: IntAct ribonuclease P activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
POP1	Q99575	1	EBI-366525,EBI-366741
POP4	O95707	1	EBI-366525,EBI-366477
RPP14	O95059	1	EBI-366525,EBI-366542
RPP25	Q9BUL9	1	EBI-366525,EBI-366570

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Ribonuclease P/MRP protein subunit POP5		PRO_0000239007
Amino acid modifications
	Modified residue	154	1	Phosphoserine Ref.7 Ref.8 Ref.9
Experimental info
	Sequence conflict	47 – 48	2	AA → P in AAF17213. Ref.2
	Sequence conflict	47 – 48	2	AA → P in AAD20966. Ref.3
	Sequence conflict	99	1	N → D in BAD96926. Ref.4
	Sequence conflict	102	1	H → R in BAD96926. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q969H6-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DF436E60B3DD50C1	FASTA	163	18,820
	10 20 30 40 50 60 MVRFKHRYLL CELVSDDPRC RLSLDDRVLS SLVRDTIARV HGTFGAAACS IGFAVRYLNA 70 80 90 100 110 120 YTGIVLLRCR KEFYQLVWSA LPFITYLENK GHRYPCFFNT LHVGGTIRTC QKFLIQYNRR 130 140 150 160 QLLILLQNCT DEGEREAIQK SVTRSCLLEE EEESGEEAAE AME

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hPop5, a protein subunit of the human RNase MRP and RNase P endoribonucleases." van Eenennaam H., Lugtenberg D., Vogelzangs J.H.P., van Venrooij W.J., Pruijn G.J.M. J. Biol. Chem. 276:31635-31641(2001) [PubMed: 11413139] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
[2]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[3]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney proximal tubule.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[6]	"Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex." Welting T.J., van Venrooij W.J., Pruijn G.J. Nucleic Acids Res. 32:2138-2146(2004) [PubMed: 15096576] [Abstract] Cited for: INTERACTION WITH POP4 AND RPP14.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, MASS SPECTROMETRY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ306296 mRNA. Translation: CAC59822.1. AF117232 mRNA. Translation: AAF17213.1. AF070660 mRNA. Translation: AAD20966.1. AK223206 mRNA. Translation: BAD96926.1. BC012505 mRNA. Translation: AAH12505.1.
IPI	IPI00107563.
RefSeq	NP_057002.2. NP_937845.1.
UniGene	Hs.279913
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969H6. 4 interactions.
STRING	Q969H6.
PTM databases
PhosphoSite	Q969H6.
Genome annotation databases
Ensembl	ENST00000341039; ENSP00000341791; ENSG00000167272; Homo sapiens. [Genome view] ENST00000357500; ENSP00000350098; ENSG00000167272; Homo sapiens. [Genome view]
GeneID	51367.
KEGG	hsa:51367.
UCSC	uc001tys.1. human.
Organism-specific databases
CTD	51367.
GeneCards	GC12M119479.
H-InvDB	HIX0011067.
HGNC	HGNC:17689. POP5.
MIM	609992. gene.
PharmGKB	PA128394660.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q969H6.
HOVERGEN	Q969H6.
OMA	CEVVSDD.
Enzyme and pathway databases
BRENDA	3.1.26.5. 247.
Gene expression databases
ArrayExpress	Q969H6.
Bgee	Q969H6.
CleanEx	HS_POP5.
Genevestigator	Q969H6.
GermOnline	ENSG00000167272. Homo sapiens.
Family and domain databases
InterPro	IPR016819. RNase_P/MRP_POP5. IPR002759. RNase_P_related. [Graphical view]
Pfam	PF01900. RNase_P_Rpp14. 1 hit. [Graphical view]
PIRSF	PIRSF023803. Ribonuclease_P_prd. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	54841.
SOURCE	Search...

Entry information

Entry name

POP5_HUMAN

Accession

Primary (citable) accession number: Q969H6
Secondary accession number(s): Q53FS5, Q9Y2Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.