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Q969H4

- CNKR1_HUMAN

UniProt

Q969H4 - CNKR1_HUMAN

Protein

Connector enhancer of kinase suppressor of ras 1

Gene

CNKSR1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    May function as an adapter protein or regulator of Ras signaling pathways.

    GO - Molecular functioni

    1. protein binding, bridging Source: MGI

    GO - Biological processi

    1. Ras protein signal transduction Source: MGI
    2. Rho protein signal transduction Source: MGI
    3. transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc

    Enzyme and pathway databases

    SignaLinkiQ969H4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Connector enhancer of kinase suppressor of ras 1
    Short name:
    Connector enhancer of KSR 1
    Alternative name(s):
    CNK homolog protein 1
    Short name:
    CNK1
    Short name:
    hCNK1
    Connector enhancer of KSR-like
    Gene namesi
    Name:CNKSR1
    Synonyms:CNK1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:19700. CNKSR1.

    Subcellular locationi

    Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cell-cell junction Source: ProtInc
    2. cell cortex Source: MGI
    3. plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi493 – 4931W → A: No interaction with Rho. 1 Publication

    Organism-specific databases

    PharmGKBiPA134901167.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Connector enhancer of kinase suppressor of ras 1PRO_0000089969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei314 – 3141Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated on tyrosine.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ969H4.
    PaxDbiQ969H4.
    PRIDEiQ969H4.

    PTM databases

    PhosphoSiteiQ969H4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ969H4.
    BgeeiQ969H4.
    CleanExiHS_CNKSR1.
    GenevestigatoriQ969H4.

    Organism-specific databases

    HPAiHPA030847.
    HPA054309.

    Interactioni

    Subunit structurei

    Interacts with RHO and RALGDS.1 Publication

    Protein-protein interaction databases

    BioGridi115550. 9 interactions.
    IntActiQ969H4. 3 interactions.
    MINTiMINT-1198255.
    STRINGi9606.ENSP00000354609.

    Structurei

    Secondary structure

    1
    720
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni4 – 63
    Turni9 – 113
    Helixi12 – 198
    Helixi21 – 244
    Helixi28 – 314
    Helixi35 – 384
    Turni43 – 453
    Helixi46 – 494
    Helixi54 – 7118
    Turni76 – 783

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WWVNMR-A1-78[»]
    ProteinModelPortaliQ969H4.
    SMRiQ969H4. Positions 1-78, 217-252, 404-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ969H4.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 7064SAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini78 – 16487CRICPROSITE-ProRule annotationAdd
    BLAST
    Domaini196 – 28590PDZPROSITE-ProRule annotationAdd
    BLAST
    Domaini403 – 502100PHPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili615 – 64632Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi285 – 37894Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CNKSR family.Curated
    Contains 1 CRIC domain.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG251550.
    HOGENOMiHOG000231154.
    HOVERGENiHBG051039.
    InParanoidiQ969H4.
    OMAiTHDVYKP.
    OrthoDBiEOG71ZP0W.
    PhylomeDBiQ969H4.
    TreeFamiTF326495.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR017874. CRIC_domain.
    IPR019555. CRIC_domain_Chordata.
    IPR001478. PDZ.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    [Graphical view]
    PfamiPF10534. CRIC_ras_sig. 1 hit.
    PF00169. PH. 1 hit.
    PF00536. SAM_1. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    PROSITEiPS51290. CRIC. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q969H4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPVETWTPG KVATWLRGLD DSLQDYPFED WQLPGKNLLQ LCPQSLEALA    50
    VRSLGHQELI LGGVEQLQAL SSRLQTENLQ SLTEGLLGAT HDFQSIVQGC 100
    LGDCAKTPID VLCAAVELLH EADALLFWLS RYLFSHLNDF SACQEIRDLL 150
    EELSQVLHED GPAAEKEGTV LRICSHVAGI CHNILVCCPK ELLEQKAVLE 200
    QVQLDSPLGL EIHTTSNCQH FVSQVDTQVP TDSRLQIQPG DEVVQINEQV 250
    VVREERDMVG WPRKNMVREL LREPAGLSLV LKKIPIPETP PQTPPQVLDS 300
    PHQRSPSLSL APLSPRAPSE DVFAFDLSSN PSPGPSPAWT DSASLGPEPL 350
    PIPPEPPAIL PAGVAGTPGL PESPDKSPVG RKKSKGLATR LSRRRVSCRE 400
    LGRPDCDGWL LLRKAPGGFM GPRWRRRWFV LKGHTLYWYR QPQDEKAEGL 450
    INVSNYSLES GHDQKKKYVF QLTHDVYKPF IFAADTLTDL SMWVRHLITC 500
    ISKYQSPGRA PPPREEDCYS ETEAEDPDDE AGSHSASPSP AQAGSPLHGD 550
    TSPAATPTQR SPRTSFGSLT DSSEEALEGM VRGLRQGGVS LLGQPQPLTQ 600
    EQWRSSFMRR NRDPQLNERV HRVRALQSTL KAKLQELQVL EEVLGDPELT 650
    GEKFRQWKEQ NRELYSEGLG AWGVAQAEGS SHILTSDSTE QSPHSLPSDP 700
    EEHSHLCPLT SESSLRPPDL 720
    Length:720
    Mass (Da):79,706
    Last modified:December 1, 2001 - v1
    Checksum:iE090785D1486D84B
    GO
    Isoform 2 (identifier: Q969H4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         253-259: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:713
    Mass (Da):78,790
    Checksum:iBF7383CCDA911E7E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti694 – 6941H → N in AAC80558. (PubMed:9814705)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti662 – 6621R → W.
    Corresponds to variant rs17163640 [ dbSNP | Ensembl ].
    VAR_057790

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei253 – 2597Missing in isoform 2. 1 PublicationVSP_010886

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF100153 mRNA. Translation: AAC80558.1.
    BT006900 mRNA. Translation: AAP35546.1.
    AL355877, AL391650 Genomic DNA. Translation: CAH71543.1.
    AL391650, AL355877 Genomic DNA. Translation: CAI17138.1.
    CH471059 Genomic DNA. Translation: EAX07842.1.
    BC011604 mRNA. Translation: AAH11604.1.
    BC012797 mRNA. Translation: AAH12797.1.
    CCDSiCCDS276.1. [Q969H4-2]
    RefSeqiNP_006305.2. NM_006314.2. [Q969H4-2]
    XP_006710344.1. XM_006710281.1. [Q969H4-1]
    UniGeneiHs.16232.

    Genome annotation databases

    EnsembliENST00000361530; ENSP00000354609; ENSG00000142675. [Q969H4-2]
    ENST00000374253; ENSP00000363371; ENSG00000142675. [Q969H4-1]
    GeneIDi10256.
    KEGGihsa:10256.
    UCSCiuc001bln.4. human. [Q969H4-1]

    Polymorphism databases

    DMDMi50400606.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF100153 mRNA. Translation: AAC80558.1 .
    BT006900 mRNA. Translation: AAP35546.1 .
    AL355877 , AL391650 Genomic DNA. Translation: CAH71543.1 .
    AL391650 , AL355877 Genomic DNA. Translation: CAI17138.1 .
    CH471059 Genomic DNA. Translation: EAX07842.1 .
    BC011604 mRNA. Translation: AAH11604.1 .
    BC012797 mRNA. Translation: AAH12797.1 .
    CCDSi CCDS276.1. [Q969H4-2 ]
    RefSeqi NP_006305.2. NM_006314.2. [Q969H4-2 ]
    XP_006710344.1. XM_006710281.1. [Q969H4-1 ]
    UniGenei Hs.16232.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WWV NMR - A 1-78 [» ]
    ProteinModelPortali Q969H4.
    SMRi Q969H4. Positions 1-78, 217-252, 404-497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115550. 9 interactions.
    IntActi Q969H4. 3 interactions.
    MINTi MINT-1198255.
    STRINGi 9606.ENSP00000354609.

    PTM databases

    PhosphoSitei Q969H4.

    Polymorphism databases

    DMDMi 50400606.

    Proteomic databases

    MaxQBi Q969H4.
    PaxDbi Q969H4.
    PRIDEi Q969H4.

    Protocols and materials databases

    DNASUi 10256.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361530 ; ENSP00000354609 ; ENSG00000142675 . [Q969H4-2 ]
    ENST00000374253 ; ENSP00000363371 ; ENSG00000142675 . [Q969H4-1 ]
    GeneIDi 10256.
    KEGGi hsa:10256.
    UCSCi uc001bln.4. human. [Q969H4-1 ]

    Organism-specific databases

    CTDi 10256.
    GeneCardsi GC01P026503.
    H-InvDB HIX0000288.
    HGNCi HGNC:19700. CNKSR1.
    HPAi HPA030847.
    HPA054309.
    MIMi 603272. gene.
    neXtProti NX_Q969H4.
    PharmGKBi PA134901167.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG251550.
    HOGENOMi HOG000231154.
    HOVERGENi HBG051039.
    InParanoidi Q969H4.
    OMAi THDVYKP.
    OrthoDBi EOG71ZP0W.
    PhylomeDBi Q969H4.
    TreeFami TF326495.

    Enzyme and pathway databases

    SignaLinki Q969H4.

    Miscellaneous databases

    EvolutionaryTracei Q969H4.
    GeneWikii CNKSR1.
    GenomeRNAii 10256.
    NextBioi 38864.
    PROi Q969H4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q969H4.
    Bgeei Q969H4.
    CleanExi HS_CNKSR1.
    Genevestigatori Q969H4.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR017874. CRIC_domain.
    IPR019555. CRIC_domain_Chordata.
    IPR001478. PDZ.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    [Graphical view ]
    Pfami PF10534. CRIC_ras_sig. 1 hit.
    PF00169. PH. 1 hit.
    PF00536. SAM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    PROSITEi PS51290. CRIC. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CNK, a RAF-binding multidomain protein required for RAS signaling."
      Therrien M., Wong A.M., Rubin G.M.
      Cell 95:343-353(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Neuroepithelium.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    6. "Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways."
      Jaffe A.B., Aspenstroem P., Hall A.
      Mol. Cell. Biol. 24:1736-1746(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RHO AND RALGDS, MUTAGENESIS OF TRP-493.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Solution structure of the SAM domain of human connector enhancer of KSR-like protein CNK1."
      RIKEN structural genomics initiative (RSGI)
      Submitted (JUL-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 1-78.

    Entry informationi

    Entry nameiCNKR1_HUMAN
    AccessioniPrimary (citable) accession number: Q969H4
    Secondary accession number(s): B1AMW9, O95381
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3