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Protein

Connector enhancer of kinase suppressor of ras 1

Gene

CNKSR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an adapter protein or regulator of Ras signaling pathways.

GO - Molecular functioni

  • protein binding, bridging Source: MGI

GO - Biological processi

  • Ras protein signal transduction Source: MGI
  • Rho protein signal transduction Source: MGI
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142675-MONOMER.
ReactomeiR-HSA-5674135. MAP2K and MAPK activation.
R-HSA-6802946. Signaling by moderate kinase activity BRAF mutants.
R-HSA-6802948. Signaling by high-kinase activity BRAF mutants.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
R-HSA-6802955. Paradoxical activation of RAF signaling by kinase inactive BRAF.
SignaLinkiQ969H4.
SIGNORiQ969H4.

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 1
Short name:
Connector enhancer of KSR 1
Alternative name(s):
CNK homolog protein 1
Short name:
CNK1
Short name:
hCNK1
Connector enhancer of KSR-like
Gene namesi
Name:CNKSR1
Synonyms:CNK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:19700. CNKSR1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: ProtInc
  • cell cortex Source: MGI
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi493W → A: No interaction with Rho. 1 Publication1

Organism-specific databases

DisGeNETi10256.
OpenTargetsiENSG00000142675.
PharmGKBiPA134901167.

Polymorphism and mutation databases

BioMutaiCNKSR1.
DMDMi50400606.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899691 – 720Connector enhancer of kinase suppressor of ras 1Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei307PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ969H4.
PaxDbiQ969H4.
PeptideAtlasiQ969H4.
PRIDEiQ969H4.

PTM databases

iPTMnetiQ969H4.
PhosphoSitePlusiQ969H4.

Expressioni

Gene expression databases

BgeeiENSG00000142675.
CleanExiHS_CNKSR1.
ExpressionAtlasiQ969H4. baseline and differential.
GenevisibleiQ969H4. HS.

Organism-specific databases

HPAiHPA030847.
HPA054309.

Interactioni

Subunit structurei

Interacts with RHO and RALGDS.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CYTH3O437394EBI-741671,EBI-741648
CYTH4Q8WWE83EBI-741671,EBI-10277443
PIN1Q135265EBI-741671,EBI-714158

GO - Molecular functioni

  • protein binding, bridging Source: MGI

Protein-protein interaction databases

BioGridi115550. 20 interactors.
IntActiQ969H4. 10 interactors.
MINTiMINT-1198255.
STRINGi9606.ENSP00000354609.

Structurei

Secondary structure

1720
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Turni9 – 11Combined sources3
Helixi12 – 19Combined sources8
Helixi21 – 24Combined sources4
Helixi28 – 31Combined sources4
Helixi35 – 38Combined sources4
Turni43 – 45Combined sources3
Helixi46 – 49Combined sources4
Helixi54 – 71Combined sources18
Turni76 – 78Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWVNMR-A1-78[»]
ProteinModelPortaliQ969H4.
SMRiQ969H4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ969H4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 70SAMPROSITE-ProRule annotationAdd BLAST64
Domaini78 – 164CRICPROSITE-ProRule annotationAdd BLAST87
Domaini196 – 285PDZPROSITE-ProRule annotationAdd BLAST90
Domaini403 – 502PHPROSITE-ProRule annotationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili615 – 646Sequence analysisAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi285 – 378Pro-richAdd BLAST94

Sequence similaritiesi

Belongs to the CNKSR family.Curated
Contains 1 CRIC domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000231154.
HOVERGENiHBG051039.
InParanoidiQ969H4.
OMAiTHDVYKP.
OrthoDBiEOG091G037J.
PhylomeDBiQ969H4.
TreeFamiTF326495.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR017874. CRIC_domain.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969H4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPVETWTPG KVATWLRGLD DSLQDYPFED WQLPGKNLLQ LCPQSLEALA
60 70 80 90 100
VRSLGHQELI LGGVEQLQAL SSRLQTENLQ SLTEGLLGAT HDFQSIVQGC
110 120 130 140 150
LGDCAKTPID VLCAAVELLH EADALLFWLS RYLFSHLNDF SACQEIRDLL
160 170 180 190 200
EELSQVLHED GPAAEKEGTV LRICSHVAGI CHNILVCCPK ELLEQKAVLE
210 220 230 240 250
QVQLDSPLGL EIHTTSNCQH FVSQVDTQVP TDSRLQIQPG DEVVQINEQV
260 270 280 290 300
VVREERDMVG WPRKNMVREL LREPAGLSLV LKKIPIPETP PQTPPQVLDS
310 320 330 340 350
PHQRSPSLSL APLSPRAPSE DVFAFDLSSN PSPGPSPAWT DSASLGPEPL
360 370 380 390 400
PIPPEPPAIL PAGVAGTPGL PESPDKSPVG RKKSKGLATR LSRRRVSCRE
410 420 430 440 450
LGRPDCDGWL LLRKAPGGFM GPRWRRRWFV LKGHTLYWYR QPQDEKAEGL
460 470 480 490 500
INVSNYSLES GHDQKKKYVF QLTHDVYKPF IFAADTLTDL SMWVRHLITC
510 520 530 540 550
ISKYQSPGRA PPPREEDCYS ETEAEDPDDE AGSHSASPSP AQAGSPLHGD
560 570 580 590 600
TSPAATPTQR SPRTSFGSLT DSSEEALEGM VRGLRQGGVS LLGQPQPLTQ
610 620 630 640 650
EQWRSSFMRR NRDPQLNERV HRVRALQSTL KAKLQELQVL EEVLGDPELT
660 670 680 690 700
GEKFRQWKEQ NRELYSEGLG AWGVAQAEGS SHILTSDSTE QSPHSLPSDP
710 720
EEHSHLCPLT SESSLRPPDL
Length:720
Mass (Da):79,706
Last modified:December 1, 2001 - v1
Checksum:iE090785D1486D84B
GO
Isoform 2 (identifier: Q969H4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     253-259: Missing.

Note: No experimental confirmation available.
Show »
Length:713
Mass (Da):78,790
Checksum:iBF7383CCDA911E7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti694H → N in AAC80558 (PubMed:9814705).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057790662R → W.Corresponds to variant rs17163640dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010886253 – 259Missing in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100153 mRNA. Translation: AAC80558.1.
BT006900 mRNA. Translation: AAP35546.1.
AL355877, AL391650 Genomic DNA. Translation: CAH71543.1.
AL391650, AL355877 Genomic DNA. Translation: CAI17138.1.
CH471059 Genomic DNA. Translation: EAX07842.1.
BC011604 mRNA. Translation: AAH11604.1.
BC012797 mRNA. Translation: AAH12797.1.
CCDSiCCDS276.1. [Q969H4-2]
CCDS72732.1. [Q969H4-1]
RefSeqiNP_001284576.1. NM_001297647.1. [Q969H4-1]
NP_001284577.1. NM_001297648.1.
NP_006305.2. NM_006314.2. [Q969H4-2]
UniGeneiHs.16232.

Genome annotation databases

EnsembliENST00000361530; ENSP00000354609; ENSG00000142675. [Q969H4-2]
ENST00000374253; ENSP00000363371; ENSG00000142675. [Q969H4-1]
GeneIDi10256.
KEGGihsa:10256.
UCSCiuc001blm.5. human. [Q969H4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100153 mRNA. Translation: AAC80558.1.
BT006900 mRNA. Translation: AAP35546.1.
AL355877, AL391650 Genomic DNA. Translation: CAH71543.1.
AL391650, AL355877 Genomic DNA. Translation: CAI17138.1.
CH471059 Genomic DNA. Translation: EAX07842.1.
BC011604 mRNA. Translation: AAH11604.1.
BC012797 mRNA. Translation: AAH12797.1.
CCDSiCCDS276.1. [Q969H4-2]
CCDS72732.1. [Q969H4-1]
RefSeqiNP_001284576.1. NM_001297647.1. [Q969H4-1]
NP_001284577.1. NM_001297648.1.
NP_006305.2. NM_006314.2. [Q969H4-2]
UniGeneiHs.16232.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWVNMR-A1-78[»]
ProteinModelPortaliQ969H4.
SMRiQ969H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115550. 20 interactors.
IntActiQ969H4. 10 interactors.
MINTiMINT-1198255.
STRINGi9606.ENSP00000354609.

PTM databases

iPTMnetiQ969H4.
PhosphoSitePlusiQ969H4.

Polymorphism and mutation databases

BioMutaiCNKSR1.
DMDMi50400606.

Proteomic databases

MaxQBiQ969H4.
PaxDbiQ969H4.
PeptideAtlasiQ969H4.
PRIDEiQ969H4.

Protocols and materials databases

DNASUi10256.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361530; ENSP00000354609; ENSG00000142675. [Q969H4-2]
ENST00000374253; ENSP00000363371; ENSG00000142675. [Q969H4-1]
GeneIDi10256.
KEGGihsa:10256.
UCSCiuc001blm.5. human. [Q969H4-1]

Organism-specific databases

CTDi10256.
DisGeNETi10256.
GeneCardsiCNKSR1.
H-InvDBHIX0000288.
HGNCiHGNC:19700. CNKSR1.
HPAiHPA030847.
HPA054309.
MIMi603272. gene.
neXtProtiNX_Q969H4.
OpenTargetsiENSG00000142675.
PharmGKBiPA134901167.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000231154.
HOVERGENiHBG051039.
InParanoidiQ969H4.
OMAiTHDVYKP.
OrthoDBiEOG091G037J.
PhylomeDBiQ969H4.
TreeFamiTF326495.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142675-MONOMER.
ReactomeiR-HSA-5674135. MAP2K and MAPK activation.
R-HSA-6802946. Signaling by moderate kinase activity BRAF mutants.
R-HSA-6802948. Signaling by high-kinase activity BRAF mutants.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
R-HSA-6802955. Paradoxical activation of RAF signaling by kinase inactive BRAF.
SignaLinkiQ969H4.
SIGNORiQ969H4.

Miscellaneous databases

ChiTaRSiCNKSR1. human.
EvolutionaryTraceiQ969H4.
GeneWikiiCNKSR1.
GenomeRNAii10256.
PROiQ969H4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142675.
CleanExiHS_CNKSR1.
ExpressionAtlasiQ969H4. baseline and differential.
GenevisibleiQ969H4. HS.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR017874. CRIC_domain.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNKR1_HUMAN
AccessioniPrimary (citable) accession number: Q969H4
Secondary accession number(s): B1AMW9, O95381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.