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Protein

Protein naked cuticle homolog 1

Gene

NKD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi144 – 15512PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • eye photoreceptor cell differentiation Source: BHF-UCL
  • negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • negative regulation of convergent extension involved in axis elongation Source: BHF-UCL
  • positive regulation of non-canonical Wnt signaling pathway via JNK cascade Source: BHF-UCL
  • positive regulation of protein catabolic process Source: BHF-UCL
  • positive regulation of Wnt signaling pathway, planar cell polarity pathway Source: BHF-UCL
  • regulation of cell motility involved in somitogenic axis elongation Source: BHF-UCL
  • somatic muscle development Source: BHF-UCL
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

SignaLinkiQ969G9.
SIGNORiQ969G9.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein naked cuticle homolog 1
Short name:
Naked-1
Short name:
hNkd
Short name:
hNkd1
Gene namesi
Name:NKD1
Synonyms:NKD
ORF Names:PP7246
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:17045. NKD1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31637.

Polymorphism and mutation databases

BioMutaiNKD1.
DMDMi74731040.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 470469Protein naked cuticle homolog 1PRO_0000301990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ969G9.
PaxDbiQ969G9.
PRIDEiQ969G9.

PTM databases

iPTMnetiQ969G9.
PhosphoSiteiQ969G9.

Expressioni

Tissue specificityi

Expressed in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine and spleen.1 Publication

Developmental stagei

Expressed in fetal brain, kidney, liver and lung.1 Publication

Inductioni

Expression is induced by activation of the Wnt signaling pathway.1 Publication

Gene expression databases

BgeeiQ969G9.
CleanExiHS_NKD1.
GenevisibleiQ969G9. HS.

Organism-specific databases

HPAiHPA041352.
HPA049413.

Interactioni

Subunit structurei

Interacts with DVL1, DVL2, DVL3 and PPP2R3A.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP2R3AQ06190-14EBI-1538217,EBI-949204
PPP2R3AQ06190-23EBI-1538217,EBI-949210

Protein-protein interaction databases

DIPiDIP-38248N.
IntActiQ969G9. 4 interactions.
STRINGi9606.ENSP00000268459.

Structurei

3D structure databases

ProteinModelPortaliQ969G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 16636EF-handPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 19066Interaction with DVL1, DVL2 and DVL3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi452 – 46615His-richAdd
BLAST

Sequence similaritiesi

Belongs to the NKD family.Curated
Contains 1 EF-hand domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IKDI. Eukaryota.
ENOG410XTFR. LUCA.
GeneTreeiENSGT00440000033589.
HOGENOMiHOG000005649.
HOVERGENiHBG099718.
InParanoidiQ969G9.
KOiK03213.
OMAiPLGHKKH.
OrthoDBiEOG75F4D6.
PhylomeDBiQ969G9.
TreeFamiTF328786.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q969G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKLHSKPAA VCKRRESPEG DSFAVSAAWA RKGIEEWIGR QRCPGGVSGP
60 70 80 90 100
RQLRLAGTIG RSTRELVGDV LRDTLSEEEE DDFRLEVALP PEKTDGLGSG
110 120 130 140 150
DEKKMERVSE PCPGSKKQLK FEELQCDVSM EEDSRQEWTF TLYDFDNNGK
160 170 180 190 200
VTREDITSLL HTIYEVVDSS VNHSPTSSKM LRVKLTVAPD GSQSKRSVLV
210 220 230 240 250
NQADLQSARP RAETKPTEDL RSWEKKQRAP LRFQGDSRLE QSGCYHHCVD
260 270 280 290 300
ENIERRNHYL DLAGIENYTS QFGPGSPSVA QKSELPPRTS NPTRSRSHEP
310 320 330 340 350
EAIHIPHRKP QGVDPASFHF LDTPIAKVSE LQQRLRGTQD GSKHFVRSPK
360 370 380 390 400
AQGKSVGVGH VARGARNKPP LGPAIPAVSP SAHLAASPAL LPSLAPLGHK
410 420 430 440 450
KHKHRAKESQ QGCRGLQAPL ASGGPVLGRE HLRELPALVV YESQAGQPVQ
460 470
RHEHHHHHEH HHHYHHFYQT
Length:470
Mass (Da):52,285
Last modified:December 1, 2001 - v1
Checksum:iE50E528042662CDE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 102AA → VD in AAL55768 (PubMed:15498874).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358135 mRNA. Translation: AAK57484.1.
AB062886 mRNA. Translation: BAB70500.1.
AY061883 mRNA. Translation: AAL32374.1.
AF289584 mRNA. Translation: AAL55768.1.
AK314930 mRNA. Translation: BAG37436.1.
CH471092 Genomic DNA. Translation: EAW82761.1.
BC051288 mRNA. Translation: AAH51288.1.
CCDSiCCDS10743.1.
RefSeqiNP_149110.1. NM_033119.4.
UniGeneiHs.187578.

Genome annotation databases

EnsembliENST00000268459; ENSP00000268459; ENSG00000140807.
GeneIDi85407.
KEGGihsa:85407.
UCSCiuc002egg.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358135 mRNA. Translation: AAK57484.1.
AB062886 mRNA. Translation: BAB70500.1.
AY061883 mRNA. Translation: AAL32374.1.
AF289584 mRNA. Translation: AAL55768.1.
AK314930 mRNA. Translation: BAG37436.1.
CH471092 Genomic DNA. Translation: EAW82761.1.
BC051288 mRNA. Translation: AAH51288.1.
CCDSiCCDS10743.1.
RefSeqiNP_149110.1. NM_033119.4.
UniGeneiHs.187578.

3D structure databases

ProteinModelPortaliQ969G9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-38248N.
IntActiQ969G9. 4 interactions.
STRINGi9606.ENSP00000268459.

PTM databases

iPTMnetiQ969G9.
PhosphoSiteiQ969G9.

Polymorphism and mutation databases

BioMutaiNKD1.
DMDMi74731040.

Proteomic databases

MaxQBiQ969G9.
PaxDbiQ969G9.
PRIDEiQ969G9.

Protocols and materials databases

DNASUi85407.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268459; ENSP00000268459; ENSG00000140807.
GeneIDi85407.
KEGGihsa:85407.
UCSCiuc002egg.3. human.

Organism-specific databases

CTDi85407.
GeneCardsiNKD1.
H-InvDBHIX0027004.
HGNCiHGNC:17045. NKD1.
HPAiHPA041352.
HPA049413.
MIMi607851. gene.
neXtProtiNX_Q969G9.
PharmGKBiPA31637.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKDI. Eukaryota.
ENOG410XTFR. LUCA.
GeneTreeiENSGT00440000033589.
HOGENOMiHOG000005649.
HOVERGENiHBG099718.
InParanoidiQ969G9.
KOiK03213.
OMAiPLGHKKH.
OrthoDBiEOG75F4D6.
PhylomeDBiQ969G9.
TreeFamiTF328786.

Enzyme and pathway databases

SignaLinkiQ969G9.
SIGNORiQ969G9.

Miscellaneous databases

ChiTaRSiNKD1. human.
GenomeRNAii85407.
PROiQ969G9.
SOURCEiSearch...

Gene expression databases

BgeeiQ969G9.
CleanExiHS_NKD1.
GenevisibleiQ969G9. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling."
    Wharton K.A. Jr., Zimmermann G., Rousset R., Scott M.P.
    Dev. Biol. 234:93-106(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning, gene structure, and expression analyses of NKD1 and NKD2."
    Katoh M.
    Int. J. Oncol. 19:963-969(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Fetus.
  3. "Elevated expression of axin2 and hnkd mRNA provides evidence that Wnt/beta-catenin signaling is activated in human colon tumors."
    Yan D., Wiesmann M., Rohan M., Chan V., Jefferson A.B., Guo L., Sakamoto D., Caothien R.H., Fuller J.H., Reinhard C., Garcia P.D., Randazzo F.M., Escobedo J., Fantl W.J., Williams L.T.
    Proc. Natl. Acad. Sci. U.S.A. 98:14973-14978(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
  4. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thalamus.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  8. "PR72, a novel regulator of Wnt signaling required for Naked cuticle function."
    Creyghton M.P., Roeel G., Eichhorn P.J.A., Hijmans E.M., Maurer I., Destree O., Bernards R.
    Genes Dev. 19:376-386(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPP2R3A.
  9. "PR130 is a modulator of the Wnt-signaling cascade that counters repression of the antagonist Naked cuticle."
    Creyghton M.P., Roeel G., Eichhorn P.J.A., Vredeveld L.C., Destree O., Bernards R.
    Proc. Natl. Acad. Sci. U.S.A. 103:5397-5402(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DVL1; DVL2; DVL3 AND PPP2R3A.

Entry informationi

Entry nameiNKD1_HUMAN
AccessioniPrimary (citable) accession number: Q969G9
Secondary accession number(s): B2RC39, Q8WZ08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.