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Q969G6

- RIFK_HUMAN

UniProt

Q969G6 - RIFK_HUMAN

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Protein

Riboflavin kinase

Gene
RFK
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.1 Publication

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zinc or magnesium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogen
Binding sitei21 – 211ATP; via amide nitrogen
Metal bindingi27 – 271Magnesium
Binding sitei27 – 271ATP; via amide nitrogen
Binding sitei29 – 291ATP
Active sitei79 – 791Nucleophile By similarity
Binding sitei82 – 821ATP; via amide nitrogen and carbonyl oxygen
Binding sitei84 – 841ATP; via carbonyl oxygen
Binding sitei91 – 911ATP
Binding sitei104 – 1041FMN
Binding sitei107 – 1071FMN; via amide nitrogen and carbonyl oxygen
Binding sitei109 – 1091FMN; via amide nitrogen

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. riboflavin kinase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: MGI
  2. FMN biosynthetic process Source: UniProtKB-UniPathway
  3. positive regulation of NAD(P)H oxidase activity Source: MGI
  4. riboflavin biosynthetic process Source: UniProtKB
  5. riboflavin metabolic process Source: Reactome
  6. small molecule metabolic process Source: Reactome
  7. vitamin metabolic process Source: Reactome
  8. water-soluble vitamin metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS05938-MONOMER.
ReactomeiREACT_11070. Vitamin B2 (riboflavin) metabolism.
UniPathwayiUPA00276; UER00406.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
ATP:riboflavin 5'-phosphotransferase
Flavokinase
Gene namesi
Name:RFK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:30324. RFK.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: Reactome
  3. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi79 – 791E → Q: Loss of kinase activity. No effect on TNFRSF1A- and CYBA-binding. 1 Publication

Organism-specific databases

PharmGKBiPA134916697.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 155155Riboflavin kinasePRO_0000194148Add
BLAST

Proteomic databases

MaxQBiQ969G6.
PaxDbiQ969G6.
PRIDEiQ969G6.

PTM databases

PhosphoSiteiQ969G6.

Expressioni

Tissue specificityi

Detected in brain, placenta and urinary bladder.

Gene expression databases

ArrayExpressiQ969G6.
BgeeiQ969G6.
CleanExiHS_RFK.
GenevestigatoriQ969G6.

Organism-specific databases

HPAiHPA057163.

Interactioni

Subunit structurei

Monomer. Directly interacts with TNFRSF1A death domain. TNFRSF1A-binding may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA.1 Publication

Protein-protein interaction databases

BioGridi120594. 3 interactions.
DIPiDIP-60454N.
IntActiQ969G6. 3 interactions.
MINTiMINT-1401441.
STRINGi9606.ENSP00000257452.

Structurei

Secondary structure

1
155
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117
Beta strandi16 – 183
Helixi20 – 234
Helixi32 – 365
Beta strandi44 – 5310
Beta strandi59 – 679
Beta strandi69 – 746
Beta strandi76 – 849
Beta strandi93 – 10412
Helixi112 – 12918
Helixi133 – 1364
Helixi137 – 1404
Helixi142 – 1465

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NB0X-ray1.70A2-148[»]
1NB9X-ray1.70A2-148[»]
1P4MX-ray1.80A2-148[»]
1Q9SX-ray2.42A1-148[»]
ProteinModelPortaliQ969G6.
SMRiQ969G6. Positions 2-148.

Miscellaneous databases

EvolutionaryTraceiQ969G6.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0196.
HOGENOMiHOG000260803.
HOVERGENiHBG049989.
InParanoidiQ969G6.
KOiK00861.
OMAiSLPYFCR.
OrthoDBiEOG7K3TP0.
PhylomeDBiQ969G6.
TreeFamiTF313786.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.

Sequencei

Sequence statusi: Complete.

Q969G6-1 [UniParc]FASTAAdd to Basket

« Hide

MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW    50
ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV 100
GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK 150
IMNGH 155
Length:155
Mass (Da):17,623
Last modified:October 14, 2008 - v2
Checksum:i3E038E487E164EBA
GO

Sequence cautioni

The sequence AAH07069.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence BAA92033.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961N → S in BAA92033. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK002011 mRNA. Translation: BAA92033.1. Different initiation.
AL391868 Genomic DNA. Translation: CAI40676.1.
BC007069 mRNA. Translation: AAH07069.1. Different initiation.
CCDSiCCDS35044.2.
RefSeqiNP_060809.3. NM_018339.5.
UniGeneiHs.37558.

Genome annotation databases

EnsembliENST00000376736; ENSP00000365926; ENSG00000135002.
GeneIDi55312.
KEGGihsa:55312.
UCSCiuc004akd.2. human.

Polymorphism databases

DMDMi209572667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK002011 mRNA. Translation: BAA92033.1 . Different initiation.
AL391868 Genomic DNA. Translation: CAI40676.1 .
BC007069 mRNA. Translation: AAH07069.1 . Different initiation.
CCDSi CCDS35044.2.
RefSeqi NP_060809.3. NM_018339.5.
UniGenei Hs.37558.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NB0 X-ray 1.70 A 2-148 [» ]
1NB9 X-ray 1.70 A 2-148 [» ]
1P4M X-ray 1.80 A 2-148 [» ]
1Q9S X-ray 2.42 A 1-148 [» ]
ProteinModelPortali Q969G6.
SMRi Q969G6. Positions 2-148.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120594. 3 interactions.
DIPi DIP-60454N.
IntActi Q969G6. 3 interactions.
MINTi MINT-1401441.
STRINGi 9606.ENSP00000257452.

Chemistry

DrugBanki DB00140. Riboflavin.

PTM databases

PhosphoSitei Q969G6.

Polymorphism databases

DMDMi 209572667.

Proteomic databases

MaxQBi Q969G6.
PaxDbi Q969G6.
PRIDEi Q969G6.

Protocols and materials databases

DNASUi 55312.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000376736 ; ENSP00000365926 ; ENSG00000135002 .
GeneIDi 55312.
KEGGi hsa:55312.
UCSCi uc004akd.2. human.

Organism-specific databases

CTDi 55312.
GeneCardsi GC09M079000.
H-InvDB HIX0169330.
HGNCi HGNC:30324. RFK.
HPAi HPA057163.
MIMi 613010. gene.
neXtProti NX_Q969G6.
PharmGKBi PA134916697.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0196.
HOGENOMi HOG000260803.
HOVERGENi HBG049989.
InParanoidi Q969G6.
KOi K00861.
OMAi SLPYFCR.
OrthoDBi EOG7K3TP0.
PhylomeDBi Q969G6.
TreeFami TF313786.

Enzyme and pathway databases

UniPathwayi UPA00276 ; UER00406 .
BioCyci MetaCyc:HS05938-MONOMER.
Reactomei REACT_11070. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

EvolutionaryTracei Q969G6.
GenomeRNAii 55312.
NextBioi 59546.
PROi Q969G6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q969G6.
Bgeei Q969G6.
CleanExi HS_RFK.
Genevestigatori Q969G6.

Family and domain databases

Gene3Di 2.40.30.30. 1 hit.
InterProi IPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view ]
PANTHERi PTHR22749. PTHR22749. 1 hit.
Pfami PF01687. Flavokinase. 1 hit.
[Graphical view ]
SMARTi SM00904. Flavokinase. 1 hit.
[Graphical view ]
SUPFAMi SSF82114. SSF82114. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Urinary bladder.
  4. Cited for: FUNCTION, INTERACTION WITH CYBA; TNFRSF1A AND TRADD, MUTAGENESIS OF GLU-79.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch."
    Karthikeyan S., Zhou Q., Mseeh F., Grishin N.V., Osterman A.L., Zhang H.
    Structure 11:265-273(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 2-148 IN COMPLEX WITH MG-ADP AND RIBOFLAVIN NUCLEOTIDE.

Entry informationi

Entry nameiRIFK_HUMAN
AccessioniPrimary (citable) accession number: Q969G6
Secondary accession number(s): Q5JSG9, Q9NUT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 14, 2008
Last modified: September 3, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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