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Protein

Thymic stromal lymphopoietin

Gene

TSLP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. Can induce allergic inflammation by directly activating mast cells.3 Publications
Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.1 Publication

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

SignaLinkiQ969D9.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymic stromal lymphopoietin
Gene namesi
Name:TSLP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:30743. TSLP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162407159.

Polymorphism and mutation databases

BioMutaiTSLP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 159131Thymic stromal lymphopoietinPRO_0000300873Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 110By similarity
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi69 ↔ 75By similarity
Disulfide bondi90 ↔ 137By similarity
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ969D9.
PRIDEiQ969D9.

PTM databases

PhosphoSiteiQ969D9.

Expressioni

Tissue specificityi

Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.2 Publications

Inductioni

Released by primary epithelial cells in response to certain microbial products, physical injury, or inflammatory cytokines.1 Publication

Gene expression databases

BgeeiQ969D9.
CleanExiHS_TSLP.
ExpressionAtlasiQ969D9. baseline and differential.
GenevisibleiQ969D9. HS.

Interactioni

Subunit structurei

Interacts with a receptor composed of CRLF2 and IL7R. Binding of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruitment of IL7R to the high-affinity ternary complex.By similarity

Protein-protein interaction databases

BioGridi124555. 3 interactions.
IntActiQ969D9. 2 interactions.
STRINGi9606.ENSP00000339804.

Structurei

3D structure databases

ProteinModelPortaliQ969D9.
SMRiQ969D9. Positions 29-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42670.
GeneTreeiENSGT00390000012541.
HOGENOMiHOG000154693.
HOVERGENiHBG094875.
InParanoidiQ969D9.
KOiK05436.
OrthoDBiEOG7R56W9.
PhylomeDBiQ969D9.
TreeFamiTF338216.

Family and domain databases

InterProiIPR029189. TSLP.
[Graphical view]
PfamiPF15216. TSLP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969D9-1) [UniParc]FASTAAdd to basket

Also known as: lfTSLP

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPFALLYVL SVSFRKIFIL QLVGLVLTYD FTNCDFEKIK AAYLSTISKD
60 70 80 90 100
LITYMSGTKS TEFNNTVSCS NRPHCLTEIQ SLTFNPTAGC ASLAKEMFAM
110 120 130 140 150
KTKAALAIWC PGYSETQINA TQAMKKRRKR KVTTNKCLEQ VSQLQGLWRR

FNRPLLKQQ
Length:159
Mass (Da):18,141
Last modified:December 1, 2001 - v1
Checksum:iFE158129406E3963
GO
Isoform 2 (identifier: Q969D9-2) [UniParc]FASTAAdd to basket

Also known as: sfTSLP

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: Missing.

Show »
Length:63
Mass (Da):7,426
Checksum:iF9B3AD49846E71B2
GO

Sequence cautioni

The sequence AAH16720.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH22894.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1111MFPFALLYVLS → MKCLGQSKKEE in AAH40592 (PubMed:15372022).CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9696Missing in isoform 2. 1 PublicationVSP_057462Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037115 mRNA. Translation: AAK67490.1.
AF338732 mRNA. Translation: AAK60617.1.
AC008572 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW49038.1.
BC016720 mRNA. Translation: AAH16720.1. Different initiation.
BC022894 mRNA. Translation: AAH22894.1. Different initiation.
BC040592 mRNA. Translation: AAH40592.1.
CCDSiCCDS4101.1. [Q969D9-1]
RefSeqiNP_149024.1. NM_033035.4. [Q969D9-1]
NP_612561.2. NM_138551.4. [Q969D9-2]
UniGeneiHs.389874.

Genome annotation databases

EnsembliENST00000344895; ENSP00000339804; ENSG00000145777. [Q969D9-1]
ENST00000379706; ENSP00000427827; ENSG00000145777. [Q969D9-2]
GeneIDi85480.
KEGGihsa:85480.
UCSCiuc003kpb.2. human. [Q969D9-1]
uc031skq.1. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037115 mRNA. Translation: AAK67490.1.
AF338732 mRNA. Translation: AAK60617.1.
AC008572 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW49038.1.
BC016720 mRNA. Translation: AAH16720.1. Different initiation.
BC022894 mRNA. Translation: AAH22894.1. Different initiation.
BC040592 mRNA. Translation: AAH40592.1.
CCDSiCCDS4101.1. [Q969D9-1]
RefSeqiNP_149024.1. NM_033035.4. [Q969D9-1]
NP_612561.2. NM_138551.4. [Q969D9-2]
UniGeneiHs.389874.

3D structure databases

ProteinModelPortaliQ969D9.
SMRiQ969D9. Positions 29-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124555. 3 interactions.
IntActiQ969D9. 2 interactions.
STRINGi9606.ENSP00000339804.

PTM databases

PhosphoSiteiQ969D9.

Polymorphism and mutation databases

BioMutaiTSLP.

Proteomic databases

PaxDbiQ969D9.
PRIDEiQ969D9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344895; ENSP00000339804; ENSG00000145777. [Q969D9-1]
ENST00000379706; ENSP00000427827; ENSG00000145777. [Q969D9-2]
GeneIDi85480.
KEGGihsa:85480.
UCSCiuc003kpb.2. human. [Q969D9-1]
uc031skq.1. human.

Organism-specific databases

CTDi85480.
GeneCardsiGC05P110405.
H-InvDBHIX0200750.
HGNCiHGNC:30743. TSLP.
MIMi607003. gene.
neXtProtiNX_Q969D9.
PharmGKBiPA162407159.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG42670.
GeneTreeiENSGT00390000012541.
HOGENOMiHOG000154693.
HOVERGENiHBG094875.
InParanoidiQ969D9.
KOiK05436.
OrthoDBiEOG7R56W9.
PhylomeDBiQ969D9.
TreeFamiTF338216.

Enzyme and pathway databases

SignaLinkiQ969D9.

Miscellaneous databases

GeneWikiiThymic_stromal_lymphopoietin.
GenomeRNAii85480.
NextBioi35517889.
PROiQ969D9.
SOURCEiSearch...

Gene expression databases

BgeeiQ969D9.
CleanExiHS_TSLP.
ExpressionAtlasiQ969D9. baseline and differential.
GenevisibleiQ969D9. HS.

Family and domain databases

InterProiIPR029189. TSLP.
[Graphical view]
PfamiPF15216. TSLP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, FUNCTION (ISOFORM 1).
  2. "Cloning of human thymic stromal lymphopoietin (TSLP) and signaling mechanisms leading to proliferation."
    Quentmeier H., Drexler H.G., Fleckenstein D., Zaborski M., Armstrong A., Sims J.E., Lyman S.D.
    Leukemia 15:1286-1292(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION (ISOFORM 1), TISSUE SPECIFICITY.
  3. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain, Lung and Testis.
  6. "Thymic stromal lymphopoietin is released by human epithelial cells in response to microbes, trauma, or inflammation and potently activates mast cells."
    Allakhverdi Z., Comeau M.R., Jessup H.K., Yoon B.R., Brewer A., Chartier S., Paquette N., Ziegler S.F., Sarfati M., Delespesse G.
    J. Exp. Med. 204:253-258(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ALLERGIC INFLAMMATION (ISOFORM 1), INDUCTION.
  7. "The short form of TSLP is constitutively translated in human keratinocytes and has characteristics of an antimicrobial peptide."
    Bjerkan L., Schreurs O., Engen S.A., Jahnsen F.L., Baekkevold E.S., Blix I.J., Schenck K.
    Mucosal Immunol. 8:49-56(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY (ISOFORM 2), ALTERNATIVE SPLICING (ISOFORM 2), FUNCTION (ISOFORM 2).

Entry informationi

Entry nameiTSLP_HUMAN
AccessioniPrimary (citable) accession number: Q969D9
Secondary accession number(s): G3XAM8, Q8IW99, Q96AU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.