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Protein

Enolase

Gene

ENO

Organism
Eimeria tenella (Coccidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (ETH_00008865)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK), Pyruvate kinase (ETH_00011145), Pyruvate kinase
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165SubstrateBy similarity1
Binding sitei174SubstrateBy similarity1
Active sitei217Proton donorBy similarity1
Metal bindingi252MagnesiumBy similarity1
Metal bindingi303MagnesiumBy similarity1
Binding sitei303SubstrateBy similarity1
Metal bindingi330MagnesiumBy similarity1
Binding sitei330SubstrateBy similarity1
Active sitei355Proton acceptorBy similarity1
Binding sitei406SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.1.11. 2046.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
OrganismiEimeria tenella (Coccidian parasite)
Taxonomic identifieri5802 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaEimeriidaeEimeria

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340881 – 445EnolaseAdd BLAST445

Proteomic databases

PRIDEiQ967Y8.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ967Y8.
SMRiQ967Y8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni382 – 385Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q967Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAIVEVKAR EILDSRGNPT VEVDLKTEQG CFRAAVPSGA STGIYEALEL
60 70 80 90 100
RDGDKTRYNG KGVLKAVENV NKVLAPALVG KDCREQAALD RLMVEELDGS
110 120 130 140 150
KNEWGWSKSV LGANAILAVS MALCRAGAAA KGIPLYKYVA QLAGHEAPEF
160 170 180 190 200
VLPVPCFNVL NGGKHAGNSL AMQEFMVAPV GARSFGEALR MGAEVYQALQ
210 220 230 240 250
RLLKAKFGLA ATNVGDEGGF APDIKDPREA LGLLVEAIRA AGHEGKVKIM
260 270 280 290 300
ADVAASEFYS KEAKSYDLDF KSPAADAHRL LTGDQLKDLF KEWSEEFPIV
310 320 330 340 350
SIEDPFDQDD FSSYAALTAE IGSKVQVVGD DLLVTNPARI RKALQHKACN
360 370 380 390 400
ALLLKVNQIG SITEAIEACK LAQASGWGVM VSHRSGETED SFIADLVVGL
410 420 430 440
RTGQIKTGAP CRSERLCKYN QLLRIEEQLQ GRCTYAGENF RNPSN
Length:445
Mass (Da):47,975
Last modified:December 1, 2001 - v1
Checksum:i9FA3B7465B860E77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353515 mRNA. Translation: AAK38886.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_EIMTE
AccessioniPrimary (citable) accession number: Q967Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: April 12, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families