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Q96725

- POLG_EBHSG

UniProt

Q96725 - POLG_EBHSG

Protein

Genome polyprotein

Gene

ORF1

Organism
European brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    NTPase presumably plays a role in replication.By similarity
    Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.By similarity
    3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved.By similarity
    RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.PROSITE-ProRule annotation
    Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.By similarity

    Catalytic activityi

    NTP + H2O = NDP + phosphate.
    Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei138 – 1392Cleavage; by 3CLproBy similarity
    Sitei334 – 3352Cleavage; by 3CLproBy similarity
    Sitei711 – 7122Cleavage; by 3CLproBy similarity
    Sitei929 – 9302Cleavage; by hostBy similarity
    Sitei986 – 9872Cleavage; by 3CLproBy similarity
    Sitei1101 – 11022Cleavage; by 3CLproBy similarity
    Active sitei1128 – 11281For 3CLpro activityBy similarity
    Active sitei1152 – 11521For 3CLpro activityBy similarity
    Active sitei1205 – 12051For 3CLpro activitySequence Analysis
    Sitei1244 – 12452Cleavage; by 3CLproBy similarity
    Sitei1760 – 17612Cleavage; by 3CLproBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. RNA-protein covalent cross-linking Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiC24.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Alternative name(s):
    p254
    Cleaved into the following 11 chains:
    Alternative name(s):
    2C-like protein
    P2C
    p37
    Alternative name(s):
    VPg
    p13
    3C-like protease (EC:3.4.22.66)
    Short name:
    3CLpro
    Alternative name(s):
    Calicivirin
    Thiol protease P3C
    p15
    Alternative name(s):
    3Dpol
    p58
    Gene namesi
    ORF Names:ORF1
    OrganismiEuropean brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
    Taxonomic identifieri316979 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeLagovirus
    Virus hostiLepus europaeus (European hare) [TaxID: 9983]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 23342334Genome polyproteinPRO_0000341986Add
    BLAST
    Chaini1 – 138138Protein p16By similarityPRO_0000036986Add
    BLAST
    Chaini139 – 334196Protein p23By similarityPRO_0000036987Add
    BLAST
    Chaini335 – 711377NTPaseBy similarityPRO_0000036988Add
    BLAST
    Chaini712 – 1108397Precursor p41PRO_0000341987Add
    BLAST
    Chaini712 – 986275Protein p29By similarityPRO_0000036989Add
    BLAST
    Chaini712 – 929218Protein p23/2PRO_0000341988Add
    BLAST
    Chaini930 – 1101172Protein p18PRO_0000341989Add
    BLAST
    Chaini987 – 1101115Viral genome-linked proteinBy similarityPRO_0000036990Add
    BLAST
    Chaini1102 – 12441433C-like proteaseBy similarityPRO_0000036991Add
    BLAST
    Chaini1245 – 1760516RNA-directed RNA polymeraseBy similarityPRO_0000036992Add
    BLAST
    Chaini1761 – 2334574Capsid protein VP60By similarityPRO_0000036994Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1007 – 10071O-(5'-phospho-RNA)-tyrosineBy similarity
    Disulfide bondi1577 ↔ 1584By similarity

    Post-translational modificationi

    Specific enzymatic cleavages by its own cysteine protease yield mature proteins. The protease cleaves itself from the nascent polyprotein autocatalytically. Precursor p41 can be cleaved by viral 3CLpro into protein p19 and VPg, or cleaved by host protease into protein p23/2 and protein p18 By similarity.By similarity
    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Disulfide bond, Phosphoprotein

    Interactioni

    Subunit structurei

    Binds to histo-blood group antigens at surface of target cells.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ96725.
    SMRiQ96725. Positions 1249-1745.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini487 – 647161SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1113 – 121199Peptidase C24Add
    BLAST
    Domaini1488 – 1612125RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 peptidase C24 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform Genome polyprotein (identifier: Q96725-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAVASRPCGV ATSVLPAKKP LSFFTDLVGK TPPRCIRAPH TLAWPVFADL     50
    DNEEESPEIC RKCGKYANGF GVFDLTDLGD VCLCSIRPQR HVGGPCCLCN 100
    KQYIRACGRY CARVLKHYKA FNKVIPCLHS RQVKPVFEGE VEDLFVELGA 150
    PTRMNFTEAE LASQGASIMD RFVDLVEPCL STEDSNFLDN ICSDASIRKR 200
    LEDEYDVDMI AAARARKDFA KTLKLALQDR ERKPDKWYSK LGCITTKGRQ 250
    WAKKVVHGAK KLSDPLKTLA AILLVALHNC VAVDTTTMLS HFKPVNLLAI 300
    LLDWTNDLPG FLTTLIRFME LYGVVQSTVN LVVDAIKSFW DRVMCATERC 350
    CDLLKRLFDK FEDSVPTGPT AGCLIFMSFV FSVIVGYLPN NSVISTFMKG 400
    AGKLTTFAGV IGAIRTLWIT INQHMVAKDI TSIQEKVMAV VKMANEAATL 450
    NQLEIVSVLC SELESTLTNR CTLPSYNQHM GVLNAAQKVV ADIHTLVLGK 500
    INMTKQRPQP VAVVFKGAPG IGKTYLVHRL AKDLGCPHPS NINFGLDHFD 550
    SYTGEDVAIA DEFNTSGDER WVELFIQMVN TNPCPLNCDK VENKNKVFSS 600
    KYLLCTTNSS MVLNATHPRA TAFYRRVIIV DVRNKAVEGW QSTRHGSKPG 650
    KHCYTKDMSH LTFQVYPHNM PAPGFVFVGE KLVKSQVAPR ELKYNELLDM 700
    IKNEHPDANF EGATKHEFVY PDVQYEQALL MWKQYFLMYG CTARLAKVFV 750
    DDIPYNQVHV ARKSDPRSPG AVHHECELKY IWRMVPHFAL GCVNMTNQLG 800
    TDLTQSQLDR ITCGVEGITV TTVDNILPFH SQNTLINPSF LKLIWALRRH 850
    LRGLRGITQV ATFIWKVMCN PVCAYDTLIR TLTGAATFSE DPVTTTIVCP 900
    NCTIQIHTCG GLLVRYSGDP APVASDNVDR GNQGIDCLTN PNLIAGFSWR 950
    QIADLFSTVM TSLCNNHLVN LATMAAIGAV ATKALQGVKG KTKRGRGARI 1000
    NLGNDEYDEW QQMRREFNNA HDMTAEEFLE LRNRAAMGSD DADAIKFRSW 1050
    WTNRQLRQDE AHVTVVGKGG VRNEVIRTRV RNAPKGPRTL DDGGFYDNDY 1100
    EGLPGYLRFN GSGWMIHIGN GMYLSNTHTA RSSCSEIVTC SPTTDLCLVK 1150
    AEPIRSVAQI AEGTPVRDWK RASITTYGLK KTFSDSTKID VLAYDGPTQT 1200
    THGDCGLPLF DEAGKVVAIH TGKLLGFSKM CTLIDCTITK GVYENTDLFC 1250
    GDPIDYRGLV AFRVAGVEPR PPVSGTRYAK VPGVPEEYHT GYRPANLGRG 1300
    DPDSHCTLMN IAVKNLQVYQ QEPKLTKVDT FIERAAADVL GFLRFLTKGE 1350
    RQMNLNFSAA FNVLDLSTSC GPFVPGKKID HVKDGKLDEV LSKHLYKCWS 1400
    VANSGKALHH VYACGLKDEL RPLDKVKEGK KRLLWGCNVG VALCAAAVFH 1450
    NLCFKLKTVA RFGPIAVGID MTSRDVDVMI TQLTSKAGDF LCLDYSKWDS 1500
    TMSPCVVRLA IDILADCCEQ TELTKSVVLT LKSLPMTVLD AMIVPTKRGL 1550
    PSGMPFTSVI NSICHWLLWS AAVYKACDEI GLFCSNLYED APFFVYGDDG 1600
    VYAMTPMMVS LLPAILDNLR DYGLSPTAAD KTEFIDVCPL KDISFLKRKF 1650
    VMSELGWLSQ LDRSSILRQL EWTKTAKRHM CIEECSELDK DERGVQLEEL 1700
    QIHAAAHGEE FFELVKKELR RQQAFTRFSV FDYQTARKTL GDRKRIVSVV 1750
    PDDSFVNVME GKPRADAPGT ATTASVPGTT TDGMDPGVVA STDVVTADNV 1800
    AASVATAGIG GPPQQASPQE SWRVNFFYND VFTWSVTDAP GSILYTVQHS 1850
    PQNNPFTQVL SQMYAGWAGG MQFRFIVAGS GIFGGRLVCA IIPPGIQIQP 1900
    GLEVRQFPHV VIDARSLEPV TITMPDLRPE MYHPTGNPGL VPTLVVSVYN 1950
    NLINPFGGTT SAIQVTVETR PSEDFEFVLI RAPSSKTVDS VNPSWLLTTP 2000
    VLTGAGSDNR WGAPIVGLQP VPGGFSTSNR HWNMNGETYG WSSPRFDDID 2050
    HPSGNVSYPS GSATNTIETW YANAGTATTN PISNIAPDGF PDMGAIPFSG 2100
    TTIPTGAWVG FGQVWNASNG TPYVGTVQAY ELGFANGAPS SIRPVTTTTG 2150
    AQLVAKSIYG VAIAQNQTSA GIIFLSKGMV STPGVAATTY TPQPSAIVTT 2200
    PGTPVAAPIG KNTPIMFSAV VRRTGDVNAG PGSANGTQYG VGSQPLSVTL 2250
    GLSLTNYSSA LQPGQFFVWQ LNFASGFMEV GMNTDGYFYA GTGAYSGMID 2300
    LTDLIDVRPV GVRPNTSTLV FNLAGVATTG YSYV 2334

    Note: Produced from the genomic RNA.

    Length:2,334
    Mass (Da):255,925
    Last modified:February 1, 1997 - v1
    Checksum:i3865CFA2457C0FB6
    GO
    Isoform Subgenomic capsid protein VP60 (identifier: Q96725-2) [UniParc]FASTAAdd to Basket

    Also known as: VP1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1758: Missing.

    Note: Produced from the subgenomic RNA.

    Show »
    Length:576
    Mass (Da):60,081
    Checksum:i037C5471E67A732C
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 17581758Missing in isoform Subgenomic capsid protein VP60. CuratedVSP_034377Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69620 Genomic RNA. Translation: CAA93445.1.
    Z32526 Genomic RNA. No translation available.
    RefSeqiNP_068828.1. NC_002615.1.

    Genome annotation databases

    GeneIDi912265.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69620 Genomic RNA. Translation: CAA93445.1 .
    Z32526 Genomic RNA. No translation available.
    RefSeqi NP_068828.1. NC_002615.1.

    3D structure databases

    ProteinModelPortali Q96725.
    SMRi Q96725. Positions 1249-1745.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C24.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 912265.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "European brown hare syndrome virus: molecular cloning and sequencing of the genome."
      Le Gall G., Huguet S., Vende P., Vautherot J.-F., Rasschaert D.
      J. Gen. Virol. 77:1693-1697(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiPOLG_EBHSG
    AccessioniPrimary (citable) accession number: Q96725
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.By similarity

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3