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Q96725

- POLG_EBHSG

UniProt

Q96725 - POLG_EBHSG

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Protein
Genome polyprotein
Gene
ORF1
Organism
European brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

NTPase presumably plays a role in replication By similarity.
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.
3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved By similarity.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.
Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei138 – 1392Cleavage; by 3CLpro By similarity
Sitei334 – 3352Cleavage; by 3CLpro By similarity
Sitei711 – 7122Cleavage; by 3CLpro By similarity
Sitei929 – 9302Cleavage; by host By similarity
Sitei986 – 9872Cleavage; by 3CLpro By similarity
Sitei1101 – 11022Cleavage; by 3CLpro By similarity
Active sitei1128 – 11281For 3CLpro activity By similarity
Active sitei1152 – 11521For 3CLpro activity By similarity
Active sitei1205 – 12051For 3CLpro activity Reviewed prediction
Sitei1244 – 12452Cleavage; by 3CLpro By similarity
Sitei1760 – 17612Cleavage; by 3CLpro By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiC24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Alternative name(s):
p254
Cleaved into the following 11 chains:
Alternative name(s):
2C-like protein
P2C
p37
Alternative name(s):
VPg
p13
3C-like protease (EC:3.4.22.66)
Short name:
3CLpro
Alternative name(s):
Calicivirin
Thiol protease P3C
p15
Alternative name(s):
3Dpol
p58
Gene namesi
ORF Names:ORF1
OrganismiEuropean brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
Taxonomic identifieri316979 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeLagovirus
Virus hostiLepus europaeus (European hare) [TaxID: 9983]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23342334Genome polyprotein
PRO_0000341986Add
BLAST
Chaini1 – 138138Protein p16 By similarity
PRO_0000036986Add
BLAST
Chaini139 – 334196Protein p23 By similarity
PRO_0000036987Add
BLAST
Chaini335 – 711377NTPase By similarity
PRO_0000036988Add
BLAST
Chaini712 – 1108397Precursor p41
PRO_0000341987Add
BLAST
Chaini712 – 986275Protein p29 By similarity
PRO_0000036989Add
BLAST
Chaini712 – 929218Protein p23/2
PRO_0000341988Add
BLAST
Chaini930 – 1101172Protein p18
PRO_0000341989Add
BLAST
Chaini987 – 1101115Viral genome-linked protein By similarity
PRO_0000036990Add
BLAST
Chaini1102 – 12441433C-like protease By similarity
PRO_0000036991Add
BLAST
Chaini1245 – 1760516RNA-directed RNA polymerase By similarity
PRO_0000036992Add
BLAST
Chaini1761 – 2334574Capsid protein VP60 By similarity
PRO_0000036994Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1007 – 10071O-(5'-phospho-RNA)-tyrosine By similarity
Disulfide bondi1577 ↔ 1584 By similarity

Post-translational modificationi

Specific enzymatic cleavages by its own cysteine protease yield mature proteins. The protease cleaves itself from the nascent polyprotein autocatalytically. Precursor p41 can be cleaved by viral 3CLpro into protein p19 and VPg, or cleaved by host protease into protein p23/2 and protein p18 By similarity.
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.

Keywords - PTMi

Covalent protein-RNA linkage, Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Binds to histo-blood group antigens at surface of target cells By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ96725.
SMRiQ96725. Positions 1249-1745.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini487 – 647161SF3 helicase
Add
BLAST
Domaini1113 – 121199Peptidase C24
Add
BLAST
Domaini1488 – 1612125RdRp catalytic
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.20. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform Genome polyprotein (identifier: Q96725-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAVASRPCGV ATSVLPAKKP LSFFTDLVGK TPPRCIRAPH TLAWPVFADL     50
DNEEESPEIC RKCGKYANGF GVFDLTDLGD VCLCSIRPQR HVGGPCCLCN 100
KQYIRACGRY CARVLKHYKA FNKVIPCLHS RQVKPVFEGE VEDLFVELGA 150
PTRMNFTEAE LASQGASIMD RFVDLVEPCL STEDSNFLDN ICSDASIRKR 200
LEDEYDVDMI AAARARKDFA KTLKLALQDR ERKPDKWYSK LGCITTKGRQ 250
WAKKVVHGAK KLSDPLKTLA AILLVALHNC VAVDTTTMLS HFKPVNLLAI 300
LLDWTNDLPG FLTTLIRFME LYGVVQSTVN LVVDAIKSFW DRVMCATERC 350
CDLLKRLFDK FEDSVPTGPT AGCLIFMSFV FSVIVGYLPN NSVISTFMKG 400
AGKLTTFAGV IGAIRTLWIT INQHMVAKDI TSIQEKVMAV VKMANEAATL 450
NQLEIVSVLC SELESTLTNR CTLPSYNQHM GVLNAAQKVV ADIHTLVLGK 500
INMTKQRPQP VAVVFKGAPG IGKTYLVHRL AKDLGCPHPS NINFGLDHFD 550
SYTGEDVAIA DEFNTSGDER WVELFIQMVN TNPCPLNCDK VENKNKVFSS 600
KYLLCTTNSS MVLNATHPRA TAFYRRVIIV DVRNKAVEGW QSTRHGSKPG 650
KHCYTKDMSH LTFQVYPHNM PAPGFVFVGE KLVKSQVAPR ELKYNELLDM 700
IKNEHPDANF EGATKHEFVY PDVQYEQALL MWKQYFLMYG CTARLAKVFV 750
DDIPYNQVHV ARKSDPRSPG AVHHECELKY IWRMVPHFAL GCVNMTNQLG 800
TDLTQSQLDR ITCGVEGITV TTVDNILPFH SQNTLINPSF LKLIWALRRH 850
LRGLRGITQV ATFIWKVMCN PVCAYDTLIR TLTGAATFSE DPVTTTIVCP 900
NCTIQIHTCG GLLVRYSGDP APVASDNVDR GNQGIDCLTN PNLIAGFSWR 950
QIADLFSTVM TSLCNNHLVN LATMAAIGAV ATKALQGVKG KTKRGRGARI 1000
NLGNDEYDEW QQMRREFNNA HDMTAEEFLE LRNRAAMGSD DADAIKFRSW 1050
WTNRQLRQDE AHVTVVGKGG VRNEVIRTRV RNAPKGPRTL DDGGFYDNDY 1100
EGLPGYLRFN GSGWMIHIGN GMYLSNTHTA RSSCSEIVTC SPTTDLCLVK 1150
AEPIRSVAQI AEGTPVRDWK RASITTYGLK KTFSDSTKID VLAYDGPTQT 1200
THGDCGLPLF DEAGKVVAIH TGKLLGFSKM CTLIDCTITK GVYENTDLFC 1250
GDPIDYRGLV AFRVAGVEPR PPVSGTRYAK VPGVPEEYHT GYRPANLGRG 1300
DPDSHCTLMN IAVKNLQVYQ QEPKLTKVDT FIERAAADVL GFLRFLTKGE 1350
RQMNLNFSAA FNVLDLSTSC GPFVPGKKID HVKDGKLDEV LSKHLYKCWS 1400
VANSGKALHH VYACGLKDEL RPLDKVKEGK KRLLWGCNVG VALCAAAVFH 1450
NLCFKLKTVA RFGPIAVGID MTSRDVDVMI TQLTSKAGDF LCLDYSKWDS 1500
TMSPCVVRLA IDILADCCEQ TELTKSVVLT LKSLPMTVLD AMIVPTKRGL 1550
PSGMPFTSVI NSICHWLLWS AAVYKACDEI GLFCSNLYED APFFVYGDDG 1600
VYAMTPMMVS LLPAILDNLR DYGLSPTAAD KTEFIDVCPL KDISFLKRKF 1650
VMSELGWLSQ LDRSSILRQL EWTKTAKRHM CIEECSELDK DERGVQLEEL 1700
QIHAAAHGEE FFELVKKELR RQQAFTRFSV FDYQTARKTL GDRKRIVSVV 1750
PDDSFVNVME GKPRADAPGT ATTASVPGTT TDGMDPGVVA STDVVTADNV 1800
AASVATAGIG GPPQQASPQE SWRVNFFYND VFTWSVTDAP GSILYTVQHS 1850
PQNNPFTQVL SQMYAGWAGG MQFRFIVAGS GIFGGRLVCA IIPPGIQIQP 1900
GLEVRQFPHV VIDARSLEPV TITMPDLRPE MYHPTGNPGL VPTLVVSVYN 1950
NLINPFGGTT SAIQVTVETR PSEDFEFVLI RAPSSKTVDS VNPSWLLTTP 2000
VLTGAGSDNR WGAPIVGLQP VPGGFSTSNR HWNMNGETYG WSSPRFDDID 2050
HPSGNVSYPS GSATNTIETW YANAGTATTN PISNIAPDGF PDMGAIPFSG 2100
TTIPTGAWVG FGQVWNASNG TPYVGTVQAY ELGFANGAPS SIRPVTTTTG 2150
AQLVAKSIYG VAIAQNQTSA GIIFLSKGMV STPGVAATTY TPQPSAIVTT 2200
PGTPVAAPIG KNTPIMFSAV VRRTGDVNAG PGSANGTQYG VGSQPLSVTL 2250
GLSLTNYSSA LQPGQFFVWQ LNFASGFMEV GMNTDGYFYA GTGAYSGMID 2300
LTDLIDVRPV GVRPNTSTLV FNLAGVATTG YSYV 2334

Note: Produced from the genomic RNA.

Length:2,334
Mass (Da):255,925
Last modified:February 1, 1997 - v1
Checksum:i3865CFA2457C0FB6
GO
Isoform Subgenomic capsid protein VP60 (identifier: Q96725-2) [UniParc]FASTAAdd to Basket

Also known as: VP1

The sequence of this isoform differs from the canonical sequence as follows:
     1-1758: Missing.

Note: Produced from the subgenomic RNA.

Show »
Length:576
Mass (Da):60,081
Checksum:i037C5471E67A732C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 17581758Missing in isoform Subgenomic capsid protein VP60.
VSP_034377Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z69620 Genomic RNA. Translation: CAA93445.1.
Z32526 Genomic RNA. No translation available.
RefSeqiNP_068828.1. NC_002615.1.

Genome annotation databases

GeneIDi912265.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z69620 Genomic RNA. Translation: CAA93445.1 .
Z32526 Genomic RNA. No translation available.
RefSeqi NP_068828.1. NC_002615.1.

3D structure databases

ProteinModelPortali Q96725.
SMRi Q96725. Positions 1249-1745.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C24.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 912265.

Family and domain databases

Gene3Di 2.60.120.20. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "European brown hare syndrome virus: molecular cloning and sequencing of the genome."
    Le Gall G., Huguet S., Vende P., Vautherot J.-F., Rasschaert D.
    J. Gen. Virol. 77:1693-1697(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLG_EBHSG
AccessioniPrimary (citable) accession number: Q96725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi