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Q96725 (POLG_EBHSG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Genome polyprotein
Alternative name(s):
p254

Cleaved into the following 11 chains:

  1. Protein p16
  2. Protein p23
  3. NTPase
    EC=3.6.1.15
    Alternative name(s):
    2C-like protein
    P2C
    p37
  4. Precursor p41
  5. Protein p29
  6. Protein p23/2
  7. Protein p18
  8. Viral genome-linked protein
    Alternative name(s):
    VPg
    p13
  9. 3C-like protease
    Short name=3CLpro
    EC=3.4.22.66
    Alternative name(s):
    Calicivirin
    Thiol protease P3C
    p15
  10. RNA-directed RNA polymerase
    EC=2.7.7.48
    Alternative name(s):
    3Dpol
    p58
  11. Capsid protein VP60
Gene names
ORF Names:ORF1
OrganismEuropean brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
Taxonomic identifier316979 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeLagovirus
Virus hostLepus europaeus (European hare) [TaxID: 9983]

Protein attributes

Sequence length2334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

NTPase presumably plays a role in replication By similarity.

Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.

3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved By similarity.

RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.

Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.

Catalytic activity

NTP + H2O = NDP + phosphate.

Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Binds to histo-blood group antigens at surface of target cells By similarity.

Subcellular location

Capsid protein VP60: Virion. Host cytoplasm By similarity.

Post-translational modification

Specific enzymatic cleavages by its own cysteine protease yield mature proteins. The protease cleaves itself from the nascent polyprotein autocatalytically. Precursor p41 can be cleaved by viral 3CLpro into protein p19 and VPg, or cleaved by host protease into protein p23/2 and protein p18 By similarity.

VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.

Miscellaneous

Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.

Sequence similarities

Contains 1 peptidase C24 domain.

Contains 1 RdRp catalytic domain.

Contains 1 SF3 helicase domain.

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform Genome polyprotein (identifier: Q96725-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced from the genomic RNA.
Isoform Subgenomic capsid protein VP60 (identifier: Q96725-2)

Also known as: VP1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1758: Missing.
Note: Produced from the subgenomic RNA.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 23342334Genome polyprotein
PRO_0000341986
Chain1 – 138138Protein p16 By similarity
PRO_0000036986
Chain139 – 334196Protein p23 By similarity
PRO_0000036987
Chain335 – 711377NTPase By similarity
PRO_0000036988
Chain712 – 1108397Precursor p41
PRO_0000341987
Chain712 – 986275Protein p29 By similarity
PRO_0000036989
Chain712 – 929218Protein p23/2
PRO_0000341988
Chain930 – 1101172Protein p18
PRO_0000341989
Chain987 – 1101115Viral genome-linked protein By similarity
PRO_0000036990
Chain1102 – 12441433C-like protease By similarity
PRO_0000036991
Chain1245 – 1760516RNA-directed RNA polymerase By similarity
PRO_0000036992
Chain1761 – 2334574Capsid protein VP60 By similarity
PRO_0000036994

Regions

Domain487 – 647161SF3 helicase
Domain1113 – 121199Peptidase C24
Domain1488 – 1612125RdRp catalytic

Sites

Active site11281For 3CLpro activity By similarity
Active site11521For 3CLpro activity By similarity
Active site12051For 3CLpro activity Potential
Site138 – 1392Cleavage; by 3CLpro By similarity
Site334 – 3352Cleavage; by 3CLpro By similarity
Site711 – 7122Cleavage; by 3CLpro By similarity
Site929 – 9302Cleavage; by host By similarity
Site986 – 9872Cleavage; by 3CLpro By similarity
Site1101 – 11022Cleavage; by 3CLpro By similarity
Site1244 – 12452Cleavage; by 3CLpro By similarity
Site1760 – 17612Cleavage; by 3CLpro By similarity

Amino acid modifications

Modified residue10071O-(5'-phospho-RNA)-tyrosine By similarity
Disulfide bond1577 ↔ 1584 By similarity

Natural variations

Alternative sequence1 – 17581758Missing in isoform Subgenomic capsid protein VP60.
VSP_034377

Sequences

Sequence LengthMass (Da)Tools
Isoform Genome polyprotein [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 3865CFA2457C0FB6

FASTA2,334255,925
        10         20         30         40         50         60 
MAVASRPCGV ATSVLPAKKP LSFFTDLVGK TPPRCIRAPH TLAWPVFADL DNEEESPEIC 

        70         80         90        100        110        120 
RKCGKYANGF GVFDLTDLGD VCLCSIRPQR HVGGPCCLCN KQYIRACGRY CARVLKHYKA 

       130        140        150        160        170        180 
FNKVIPCLHS RQVKPVFEGE VEDLFVELGA PTRMNFTEAE LASQGASIMD RFVDLVEPCL 

       190        200        210        220        230        240 
STEDSNFLDN ICSDASIRKR LEDEYDVDMI AAARARKDFA KTLKLALQDR ERKPDKWYSK 

       250        260        270        280        290        300 
LGCITTKGRQ WAKKVVHGAK KLSDPLKTLA AILLVALHNC VAVDTTTMLS HFKPVNLLAI 

       310        320        330        340        350        360 
LLDWTNDLPG FLTTLIRFME LYGVVQSTVN LVVDAIKSFW DRVMCATERC CDLLKRLFDK 

       370        380        390        400        410        420 
FEDSVPTGPT AGCLIFMSFV FSVIVGYLPN NSVISTFMKG AGKLTTFAGV IGAIRTLWIT 

       430        440        450        460        470        480 
INQHMVAKDI TSIQEKVMAV VKMANEAATL NQLEIVSVLC SELESTLTNR CTLPSYNQHM 

       490        500        510        520        530        540 
GVLNAAQKVV ADIHTLVLGK INMTKQRPQP VAVVFKGAPG IGKTYLVHRL AKDLGCPHPS 

       550        560        570        580        590        600 
NINFGLDHFD SYTGEDVAIA DEFNTSGDER WVELFIQMVN TNPCPLNCDK VENKNKVFSS 

       610        620        630        640        650        660 
KYLLCTTNSS MVLNATHPRA TAFYRRVIIV DVRNKAVEGW QSTRHGSKPG KHCYTKDMSH 

       670        680        690        700        710        720 
LTFQVYPHNM PAPGFVFVGE KLVKSQVAPR ELKYNELLDM IKNEHPDANF EGATKHEFVY 

       730        740        750        760        770        780 
PDVQYEQALL MWKQYFLMYG CTARLAKVFV DDIPYNQVHV ARKSDPRSPG AVHHECELKY 

       790        800        810        820        830        840 
IWRMVPHFAL GCVNMTNQLG TDLTQSQLDR ITCGVEGITV TTVDNILPFH SQNTLINPSF 

       850        860        870        880        890        900 
LKLIWALRRH LRGLRGITQV ATFIWKVMCN PVCAYDTLIR TLTGAATFSE DPVTTTIVCP 

       910        920        930        940        950        960 
NCTIQIHTCG GLLVRYSGDP APVASDNVDR GNQGIDCLTN PNLIAGFSWR QIADLFSTVM 

       970        980        990       1000       1010       1020 
TSLCNNHLVN LATMAAIGAV ATKALQGVKG KTKRGRGARI NLGNDEYDEW QQMRREFNNA 

      1030       1040       1050       1060       1070       1080 
HDMTAEEFLE LRNRAAMGSD DADAIKFRSW WTNRQLRQDE AHVTVVGKGG VRNEVIRTRV 

      1090       1100       1110       1120       1130       1140 
RNAPKGPRTL DDGGFYDNDY EGLPGYLRFN GSGWMIHIGN GMYLSNTHTA RSSCSEIVTC 

      1150       1160       1170       1180       1190       1200 
SPTTDLCLVK AEPIRSVAQI AEGTPVRDWK RASITTYGLK KTFSDSTKID VLAYDGPTQT 

      1210       1220       1230       1240       1250       1260 
THGDCGLPLF DEAGKVVAIH TGKLLGFSKM CTLIDCTITK GVYENTDLFC GDPIDYRGLV 

      1270       1280       1290       1300       1310       1320 
AFRVAGVEPR PPVSGTRYAK VPGVPEEYHT GYRPANLGRG DPDSHCTLMN IAVKNLQVYQ 

      1330       1340       1350       1360       1370       1380 
QEPKLTKVDT FIERAAADVL GFLRFLTKGE RQMNLNFSAA FNVLDLSTSC GPFVPGKKID 

      1390       1400       1410       1420       1430       1440 
HVKDGKLDEV LSKHLYKCWS VANSGKALHH VYACGLKDEL RPLDKVKEGK KRLLWGCNVG 

      1450       1460       1470       1480       1490       1500 
VALCAAAVFH NLCFKLKTVA RFGPIAVGID MTSRDVDVMI TQLTSKAGDF LCLDYSKWDS 

      1510       1520       1530       1540       1550       1560 
TMSPCVVRLA IDILADCCEQ TELTKSVVLT LKSLPMTVLD AMIVPTKRGL PSGMPFTSVI 

      1570       1580       1590       1600       1610       1620 
NSICHWLLWS AAVYKACDEI GLFCSNLYED APFFVYGDDG VYAMTPMMVS LLPAILDNLR 

      1630       1640       1650       1660       1670       1680 
DYGLSPTAAD KTEFIDVCPL KDISFLKRKF VMSELGWLSQ LDRSSILRQL EWTKTAKRHM 

      1690       1700       1710       1720       1730       1740 
CIEECSELDK DERGVQLEEL QIHAAAHGEE FFELVKKELR RQQAFTRFSV FDYQTARKTL 

      1750       1760       1770       1780       1790       1800 
GDRKRIVSVV PDDSFVNVME GKPRADAPGT ATTASVPGTT TDGMDPGVVA STDVVTADNV 

      1810       1820       1830       1840       1850       1860 
AASVATAGIG GPPQQASPQE SWRVNFFYND VFTWSVTDAP GSILYTVQHS PQNNPFTQVL 

      1870       1880       1890       1900       1910       1920 
SQMYAGWAGG MQFRFIVAGS GIFGGRLVCA IIPPGIQIQP GLEVRQFPHV VIDARSLEPV 

      1930       1940       1950       1960       1970       1980 
TITMPDLRPE MYHPTGNPGL VPTLVVSVYN NLINPFGGTT SAIQVTVETR PSEDFEFVLI 

      1990       2000       2010       2020       2030       2040 
RAPSSKTVDS VNPSWLLTTP VLTGAGSDNR WGAPIVGLQP VPGGFSTSNR HWNMNGETYG 

      2050       2060       2070       2080       2090       2100 
WSSPRFDDID HPSGNVSYPS GSATNTIETW YANAGTATTN PISNIAPDGF PDMGAIPFSG 

      2110       2120       2130       2140       2150       2160 
TTIPTGAWVG FGQVWNASNG TPYVGTVQAY ELGFANGAPS SIRPVTTTTG AQLVAKSIYG 

      2170       2180       2190       2200       2210       2220 
VAIAQNQTSA GIIFLSKGMV STPGVAATTY TPQPSAIVTT PGTPVAAPIG KNTPIMFSAV 

      2230       2240       2250       2260       2270       2280 
VRRTGDVNAG PGSANGTQYG VGSQPLSVTL GLSLTNYSSA LQPGQFFVWQ LNFASGFMEV 

      2290       2300       2310       2320       2330 
GMNTDGYFYA GTGAYSGMID LTDLIDVRPV GVRPNTSTLV FNLAGVATTG YSYV 

« Hide

Isoform Subgenomic capsid protein VP60 (VP1) [UniParc].

Checksum: 037C5471E67A732C
Show »

FASTA57660,081

References

[1]"European brown hare syndrome virus: molecular cloning and sequencing of the genome."
Le Gall G., Huguet S., Vende P., Vautherot J.-F., Rasschaert D.
J. Gen. Virol. 77:1693-1697(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z69620 Genomic RNA. Translation: CAA93445.1.
Z32526 Genomic RNA. No translation available.
RefSeqNP_068828.1. NC_002615.1.

3D structure databases

ProteinModelPortalQ96725.
SMRQ96725. Positions 1249-1745.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC24.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID912265.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOLG_EBHSG
AccessionPrimary (citable) accession number: Q96725
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries