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Q96725

- POLG_EBHSG

UniProt

Q96725 - POLG_EBHSG

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Protein

Genome polyprotein

Gene

ORF1

Organism
European brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

NTPase presumably plays a role in replication.By similarity
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation (By similarity).By similarity
3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved.By similarity
RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs (By similarity).PROSITE-ProRule annotation
Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm (By similarity).By similarity

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei138 – 1392Cleavage; by 3CLproBy similarity
Sitei334 – 3352Cleavage; by 3CLproBy similarity
Sitei711 – 7122Cleavage; by 3CLproBy similarity
Sitei929 – 9302Cleavage; by hostBy similarity
Sitei986 – 9872Cleavage; by 3CLproBy similarity
Sitei1101 – 11022Cleavage; by 3CLproBy similarity
Active sitei1128 – 11281For 3CLpro activityBy similarity
Active sitei1152 – 11521For 3CLpro activityBy similarity
Active sitei1205 – 12051For 3CLpro activitySequence Analysis
Sitei1244 – 12452Cleavage; by 3CLproBy similarity
Sitei1760 – 17612Cleavage; by 3CLproBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. RNA binding Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiC24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Alternative name(s):
p254
Cleaved into the following 11 chains:
Alternative name(s):
2C-like protein
P2C
p37
Alternative name(s):
VPg
p13
3C-like protease (EC:3.4.22.66)
Short name:
3CLpro
Alternative name(s):
Calicivirin
Thiol protease P3C
p15
Alternative name(s):
3Dpol
p58
Gene namesi
ORF Names:ORF1
OrganismiEuropean brown hare syndrome virus (strain GD) (Ha/LV/EBHSV/GD/1989/FR) (EBHSV-GD)
Taxonomic identifieri316979 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeLagovirus
Virus hostiLepus europaeus (European hare) [TaxID: 9983]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23342334Genome polyproteinPRO_0000341986Add
BLAST
Chaini1 – 138138Protein p16By similarityPRO_0000036986Add
BLAST
Chaini139 – 334196Protein p23By similarityPRO_0000036987Add
BLAST
Chaini335 – 711377NTPaseBy similarityPRO_0000036988Add
BLAST
Chaini712 – 1108397Precursor p41PRO_0000341987Add
BLAST
Chaini712 – 986275Protein p29By similarityPRO_0000036989Add
BLAST
Chaini712 – 929218Protein p23/2PRO_0000341988Add
BLAST
Chaini930 – 1101172Protein p18PRO_0000341989Add
BLAST
Chaini987 – 1101115Viral genome-linked proteinBy similarityPRO_0000036990Add
BLAST
Chaini1102 – 12441433C-like proteaseBy similarityPRO_0000036991Add
BLAST
Chaini1245 – 1760516RNA-directed RNA polymeraseBy similarityPRO_0000036992Add
BLAST
Chaini1761 – 2334574Capsid protein VP60By similarityPRO_0000036994Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1007 – 10071O-(5'-phospho-RNA)-tyrosineBy similarity
Disulfide bondi1577 ↔ 1584By similarity

Post-translational modificationi

Specific enzymatic cleavages by its own cysteine protease yield mature proteins. The protease cleaves itself from the nascent polyprotein autocatalytically. Precursor p41 can be cleaved by viral 3CLpro into protein p19 and VPg, or cleaved by host protease into protein p23/2 and protein p18 (By similarity).By similarity
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity

Keywords - PTMi

Covalent protein-RNA linkage, Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Binds to histo-blood group antigens at surface of target cells.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ96725.
SMRiQ96725. Positions 1249-1745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini487 – 647161SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1113 – 121199Peptidase C24Add
BLAST
Domaini1488 – 1612125RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 peptidase C24 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.20. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform Genome polyprotein (identifier: Q96725-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVASRPCGV ATSVLPAKKP LSFFTDLVGK TPPRCIRAPH TLAWPVFADL
60 70 80 90 100
DNEEESPEIC RKCGKYANGF GVFDLTDLGD VCLCSIRPQR HVGGPCCLCN
110 120 130 140 150
KQYIRACGRY CARVLKHYKA FNKVIPCLHS RQVKPVFEGE VEDLFVELGA
160 170 180 190 200
PTRMNFTEAE LASQGASIMD RFVDLVEPCL STEDSNFLDN ICSDASIRKR
210 220 230 240 250
LEDEYDVDMI AAARARKDFA KTLKLALQDR ERKPDKWYSK LGCITTKGRQ
260 270 280 290 300
WAKKVVHGAK KLSDPLKTLA AILLVALHNC VAVDTTTMLS HFKPVNLLAI
310 320 330 340 350
LLDWTNDLPG FLTTLIRFME LYGVVQSTVN LVVDAIKSFW DRVMCATERC
360 370 380 390 400
CDLLKRLFDK FEDSVPTGPT AGCLIFMSFV FSVIVGYLPN NSVISTFMKG
410 420 430 440 450
AGKLTTFAGV IGAIRTLWIT INQHMVAKDI TSIQEKVMAV VKMANEAATL
460 470 480 490 500
NQLEIVSVLC SELESTLTNR CTLPSYNQHM GVLNAAQKVV ADIHTLVLGK
510 520 530 540 550
INMTKQRPQP VAVVFKGAPG IGKTYLVHRL AKDLGCPHPS NINFGLDHFD
560 570 580 590 600
SYTGEDVAIA DEFNTSGDER WVELFIQMVN TNPCPLNCDK VENKNKVFSS
610 620 630 640 650
KYLLCTTNSS MVLNATHPRA TAFYRRVIIV DVRNKAVEGW QSTRHGSKPG
660 670 680 690 700
KHCYTKDMSH LTFQVYPHNM PAPGFVFVGE KLVKSQVAPR ELKYNELLDM
710 720 730 740 750
IKNEHPDANF EGATKHEFVY PDVQYEQALL MWKQYFLMYG CTARLAKVFV
760 770 780 790 800
DDIPYNQVHV ARKSDPRSPG AVHHECELKY IWRMVPHFAL GCVNMTNQLG
810 820 830 840 850
TDLTQSQLDR ITCGVEGITV TTVDNILPFH SQNTLINPSF LKLIWALRRH
860 870 880 890 900
LRGLRGITQV ATFIWKVMCN PVCAYDTLIR TLTGAATFSE DPVTTTIVCP
910 920 930 940 950
NCTIQIHTCG GLLVRYSGDP APVASDNVDR GNQGIDCLTN PNLIAGFSWR
960 970 980 990 1000
QIADLFSTVM TSLCNNHLVN LATMAAIGAV ATKALQGVKG KTKRGRGARI
1010 1020 1030 1040 1050
NLGNDEYDEW QQMRREFNNA HDMTAEEFLE LRNRAAMGSD DADAIKFRSW
1060 1070 1080 1090 1100
WTNRQLRQDE AHVTVVGKGG VRNEVIRTRV RNAPKGPRTL DDGGFYDNDY
1110 1120 1130 1140 1150
EGLPGYLRFN GSGWMIHIGN GMYLSNTHTA RSSCSEIVTC SPTTDLCLVK
1160 1170 1180 1190 1200
AEPIRSVAQI AEGTPVRDWK RASITTYGLK KTFSDSTKID VLAYDGPTQT
1210 1220 1230 1240 1250
THGDCGLPLF DEAGKVVAIH TGKLLGFSKM CTLIDCTITK GVYENTDLFC
1260 1270 1280 1290 1300
GDPIDYRGLV AFRVAGVEPR PPVSGTRYAK VPGVPEEYHT GYRPANLGRG
1310 1320 1330 1340 1350
DPDSHCTLMN IAVKNLQVYQ QEPKLTKVDT FIERAAADVL GFLRFLTKGE
1360 1370 1380 1390 1400
RQMNLNFSAA FNVLDLSTSC GPFVPGKKID HVKDGKLDEV LSKHLYKCWS
1410 1420 1430 1440 1450
VANSGKALHH VYACGLKDEL RPLDKVKEGK KRLLWGCNVG VALCAAAVFH
1460 1470 1480 1490 1500
NLCFKLKTVA RFGPIAVGID MTSRDVDVMI TQLTSKAGDF LCLDYSKWDS
1510 1520 1530 1540 1550
TMSPCVVRLA IDILADCCEQ TELTKSVVLT LKSLPMTVLD AMIVPTKRGL
1560 1570 1580 1590 1600
PSGMPFTSVI NSICHWLLWS AAVYKACDEI GLFCSNLYED APFFVYGDDG
1610 1620 1630 1640 1650
VYAMTPMMVS LLPAILDNLR DYGLSPTAAD KTEFIDVCPL KDISFLKRKF
1660 1670 1680 1690 1700
VMSELGWLSQ LDRSSILRQL EWTKTAKRHM CIEECSELDK DERGVQLEEL
1710 1720 1730 1740 1750
QIHAAAHGEE FFELVKKELR RQQAFTRFSV FDYQTARKTL GDRKRIVSVV
1760 1770 1780 1790 1800
PDDSFVNVME GKPRADAPGT ATTASVPGTT TDGMDPGVVA STDVVTADNV
1810 1820 1830 1840 1850
AASVATAGIG GPPQQASPQE SWRVNFFYND VFTWSVTDAP GSILYTVQHS
1860 1870 1880 1890 1900
PQNNPFTQVL SQMYAGWAGG MQFRFIVAGS GIFGGRLVCA IIPPGIQIQP
1910 1920 1930 1940 1950
GLEVRQFPHV VIDARSLEPV TITMPDLRPE MYHPTGNPGL VPTLVVSVYN
1960 1970 1980 1990 2000
NLINPFGGTT SAIQVTVETR PSEDFEFVLI RAPSSKTVDS VNPSWLLTTP
2010 2020 2030 2040 2050
VLTGAGSDNR WGAPIVGLQP VPGGFSTSNR HWNMNGETYG WSSPRFDDID
2060 2070 2080 2090 2100
HPSGNVSYPS GSATNTIETW YANAGTATTN PISNIAPDGF PDMGAIPFSG
2110 2120 2130 2140 2150
TTIPTGAWVG FGQVWNASNG TPYVGTVQAY ELGFANGAPS SIRPVTTTTG
2160 2170 2180 2190 2200
AQLVAKSIYG VAIAQNQTSA GIIFLSKGMV STPGVAATTY TPQPSAIVTT
2210 2220 2230 2240 2250
PGTPVAAPIG KNTPIMFSAV VRRTGDVNAG PGSANGTQYG VGSQPLSVTL
2260 2270 2280 2290 2300
GLSLTNYSSA LQPGQFFVWQ LNFASGFMEV GMNTDGYFYA GTGAYSGMID
2310 2320 2330
LTDLIDVRPV GVRPNTSTLV FNLAGVATTG YSYV

Note: Produced from the genomic RNA.

Length:2,334
Mass (Da):255,925
Last modified:February 1, 1997 - v1
Checksum:i3865CFA2457C0FB6
GO
Isoform Subgenomic capsid protein VP60 (identifier: Q96725-2) [UniParc]FASTAAdd to Basket

Also known as: VP1

The sequence of this isoform differs from the canonical sequence as follows:
     1-1758: Missing.

Note: Produced from the subgenomic RNA.

Show »
Length:576
Mass (Da):60,081
Checksum:i037C5471E67A732C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 17581758Missing in isoform Subgenomic capsid protein VP60. CuratedVSP_034377Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z69620 Genomic RNA. Translation: CAA93445.1.
Z32526 Genomic RNA. No translation available.
RefSeqiNP_068828.1. NC_002615.1.

Genome annotation databases

GeneIDi912265.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z69620 Genomic RNA. Translation: CAA93445.1 .
Z32526 Genomic RNA. No translation available.
RefSeqi NP_068828.1. NC_002615.1.

3D structure databases

ProteinModelPortali Q96725.
SMRi Q96725. Positions 1249-1745.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C24.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 912265.

Family and domain databases

Gene3Di 2.60.120.20. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "European brown hare syndrome virus: molecular cloning and sequencing of the genome."
    Le Gall G., Huguet S., Vende P., Vautherot J.-F., Rasschaert D.
    J. Gen. Virol. 77:1693-1697(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLG_EBHSG
AccessioniPrimary (citable) accession number: Q96725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one (By similarity).By similarity

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3