Q96711 (RDRP_CMVKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase 2a
Short name=protein 2a
EC=2.7.7.48

Gene names

ORF Names:

ORF2a

Organism

Cucumber mosaic virus (strain Kor) (CMV)

Taxonomic identifier

117116 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Cucumovirus

Virus host

Spinacia oleracea (Spinach) [TaxID: 3562]
Cucumis sativus (Cucumber) [TaxID: 3659]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with replication protein 1a By similarity.
Sequence similarities	Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 857	857	RNA-directed RNA polymerase 2a		PRO_0000083277
Regions
	Domain	511 – 624	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q96711 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 9E26A3AD064DAE04	FASTA	857	96,716
	10 20 30 40 50 60 MASPAPAFSL ANLLNGSYGV DTPEDVERLR SEQREEAAAA CRNYRPLPAV DVSESVTEDA 70 80 90 100 110 120 HSLRTPDGAP AEAVSDEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF 130 140 150 160 170 180 ISSIAMARAL LLAPRTSNRT MKCFEDLVAA IYTKSDFYYG EECEADDVQM DISSRDVPGY 190 200 210 220 230 240 SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA 250 260 270 280 290 300 TLLSNLGPFL VPVKCQYENC PTPTLAIPPD LNRATDRVDI NLVQSICDST LPTHSNYDDS 310 320 330 340 350 360 FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK 370 380 390 400 410 420 RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS 430 440 450 460 470 480 YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKSITS QFSPLFTALF 490 500 510 520 530 540 ERFQRCLRER IILPVGKISS LEMAGFDVKN KHCLEIDLSK FDKSQGEFHL LIQEHILNGL 550 560 570 580 590 600 GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD 610 620 630 640 650 660 QFEKLLFSGD DSLGFSLLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS 670 680 690 700 710 720 VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMSQSCIDQL SIFFELKYKK 730 740 750 760 770 780 SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFC STEFRVERVN SNKQRKKYGI 790 800 810 820 830 840 ERRCNDKRRT PTGSYGGGEE AETKISQAES TGTRSQKSQR ESAFKSQTVP LPTVLSSRWF 850 GTDRVEPPCE RGGVTRA

References

[1]	"Full-length cDNA cloning and nucleotide sequence analysis of cucumber mosaic virus (strain Kor) RNA2." Kwon C.S., Paek K.H., Chung W.I. J. Plant Biol. 39:265-271(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66287 Genomic RNA. Translation: AAB07139.1.
3D structure databases
ProteinModelPortal	Q96711.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_CMVKO

Accession

Primary (citable) accession number: Q96711

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 28, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program