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Protein

Nuclear shuttle protein

Gene

BR1

Organism
Cabbage leaf curl virus (isolate Jamaica) (CaLCuV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the genomic viral ssDNA, shuttles it into and out of the cell nucleus. Begomoviruses use 2 proteins to transport their DNA from cell to cell. The nuclear shuttle protein (NSP) shuttles it between nucleus and cytoplasm and the movement protein (MP) probably transports the DNA-NSP complex to the cell periphery and facilitates movement across the cell wall (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Viral movement protein

Keywords - Biological processi

Host-virus interaction, Transport

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear shuttle protein
Short name:
NSP
Alternative name(s):
Protein BR1
Protein BV1
Gene namesi
ORF Names:BR1, BV1
OrganismiCabbage leaf curl virus (isolate Jamaica) (CaLCuV)
Taxonomic identifieri345184 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeBegomovirus
Virus hostiBrassica oleracea (Wild cabbage) [TaxID: 3712]
Proteomesi
  • UP000007622 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host cytoplasm, Host membrane, Host nucleus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi150 – 1501E → G: Complete loss of interaction with Arabidopsis thaliana NSI protein. Reduced level of infectivity. 1 Publication
Mutagenesisi164 – 1641I → T: Complete loss of interaction with Arabidopsis thaliana NSI protein. Reduced level of infectivity. 1 Publication
Mutagenesisi187 – 1871D → G: Complete loss of interaction with Arabidopsis thaliana NSI protein. Reduced level of infectivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Nuclear shuttle proteinPRO_0000323698Add
BLAST

Interactioni

Subunit structurei

Binds to single-stranded and double-stranded viral DNA. Interacts with the host nuclear shuttle interacting (NSI) protein. This interaction may allow NSP to recruit NSI monomers to the viral genome and thus regulate nuclear export of viral genome by NSP.3 Publications

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 18738Interaction with Arabidopsis thaliana NSI proteinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 4222Bipartite nuclear localization signalBy similarityAdd
BLAST
Motifi81 – 9616Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001530. Gemini_BR1.
IPR000263. GV_A/BR1_coat.
[Graphical view]
PfamiPF00844. Gemini_coat. 1 hit.
[Graphical view]
PRINTSiPR00223. GEMCOATARBR1.
PR00225. GEMCOATBR1.

Sequencei

Sequence statusi: Complete.

Q96706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPTKFRRGV SYSQRRFVSR NQSSKRGTFV RRTDGKRRKG PSSKAHDEPK
60 70 80 90 100
MKLQRIHENQ YGPEFVMTHN SALSTFINFP VLGKIEPNRS RSYIKLNRLS
110 120 130 140 150
FKGTVKIERV HADVNMDGVI SKIEGVFSLV IVVDRKPHLS STGGLHTFDE
160 170 180 190 200
IFGARIHSHG NLAITPGLKD RYYVLHVLKR VLSVEKDTLM VDLEGSTTIS
210 220 230 240 250
NRRYNCWASF NDLEHDLCNG VYANISKNAI LVYYCWMSDA MSKASTFVSY

DLDYLG
Length:256
Mass (Da):29,278
Last modified:February 1, 1997 - v1
Checksum:i0C2E8E0DB4D2B748
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65530 Genomic DNA. Translation: AAB17965.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65530 Genomic DNA. Translation: AAB17965.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001530. Gemini_BR1.
IPR000263. GV_A/BR1_coat.
[Graphical view]
PfamiPF00844. Gemini_coat. 1 hit.
[Graphical view]
PRINTSiPR00223. GEMCOATARBR1.
PR00225. GEMCOATBR1.
ProtoNetiSearch...

Publicationsi

  1. "Cloning, identification and partial sequencing of a new geminivirus infecting Brassicaceae."
    Abouzid A.M., Hiebert E., Strandberg J.O.
    Phytopathology 82:1070-1070(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP."
    McGarry R.C., Barron Y.D., Carvalho M.F., Hill J.E., Gold D., Cheung E., Kraus W.L., Lazarowitz S.G.
    Plant Cell 15:1605-1618(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARABIDOPSIS THALIANA NSI.
  3. "Interaction of the movement protein NSP and the Arabidopsis acetyltransferase AtNSI is necessary for Cabbage leaf curl geminivirus infection and pathogenicity."
    Carvalho M.F., Lazarowitz S.G.
    J. Virol. 78:11161-11171(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARABIDOPSIS THALIANA NSI, MUTAGENESIS OF GLU-150; ILE-164 AND ASP-187.
  4. "The geminivirus nuclear shuttle protein NSP inhibits the activity of AtNSI, a vascular-expressed Arabidopsis acetyltransferase regulated with the sink-to-source transition."
    Carvalho M.F., Turgeon R., Lazarowitz S.G.
    Plant Physiol. 140:1317-1330(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARABIDOPSIS THALIANA NSI.

Entry informationi

Entry nameiNSP_CALCV
AccessioniPrimary (citable) accession number: Q96706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: February 1, 1997
Last modified: November 11, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.