Reviewed, UniProtKB/Swiss-Prot Q966Y3 (JNK_SUBDO)

Names and origin

Protein names

Recommended name:
    Stress-activated protein kinase JNK
    EC=2.7.11.24

Gene names

Name:

JNK

Organism

Suberites domuncula (Sponge)

Taxonomic identifier

55567 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Porifera › Demospongiae › Tetractinomorpha › Hadromerida › Suberitidae › Suberites

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulates transcriptional activity By similarity. UniProtKB P45983
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. UniProtKB P45983
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB P45983
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-179 and Tyr-181, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	JNK cascade Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Stress-activated protein kinase JNK		PRO_0000186272
Regions
	Domain	22 – 317	296	Protein kinase UniProtKB P53779
	Nucleotide binding	29 – 34	6	ATP By similarity UniProtKB P53779
	Motif	179 – 181	3	TXY
Sites
	Active site	147	1	Proton acceptor By similarity UniProtKB P53779
	Binding site	51	1	ATP By similarity UniProtKB P53779
Amino acid modifications
	Modified residue	179	1	Phosphothreonine By similarity UniProtKB P53779
	Modified residue	181	1	Phosphotyrosine By similarity UniProtKB P53779

Sequences

	Sequence		Length	Mass (Da)
	Q966Y3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5CAA9A360CDAA744	FASTA	361	41,566
	10 20 30 40 50 60 MSSSDYYSQR VGDTVFTVQK RYTNLTNIGS GAQGVVCSAF DTVTQEKIAI KKLVKPFQNE 70 80 90 100 110 120 TYAKRAFREL RLMKMVDHKN IIGLKNLFTP AKSLDDFQDV YIVMELMDAN LCRVIGIELD 130 140 150 160 170 180 HDRMSYLLYQ LLCGIKHLHS AGIIHRDLKP SNIVVKEDCS LKILDFGLAR TADQTFNMTP 190 200 210 220 230 240 YVVTRYYRAP EVIVGMKYKE NVDIWSVGCI FAEMIRGDIL LPGKDYIDQW NKVTQVLGTP 250 260 270 280 290 300 PSVFFKQLSS SVRLYCESQP RYAGKSWKDL FPDDVFPNDT PEDKAKTRHG RDLLSKMLQI 310 320 330 340 350 360 DPQNRITVEQ ALAHPYVSIW YDPAEVHAPP PKRYDHALDE QSIPLDQWKT RIYEEVKTYN S

References

[1]	"Molecular response of the sponge Suberites domuncula to bacterial infection." Boehm M., Hentschel U., Friedrich A., Steffen R., Pahler S., Gamulin V., Mueller I.M., Mueller W.E.G. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Conservation of the positions of metazoan introns from sponges to humans." Mueller W.E.G., Boehm M., Grebenjuk V.A., Skorokhod A., Mueller I.M., Gamulin V. Gene 295:299-309(2002) [PubMed: 12354665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ291511 mRNA. Translation: CAC38785.1. AJ307673 Genomic DNA. Translation: CAC85496.1.
3D structure databases
HSSP	HSSP built from PDB template 1JNK based on UniProtKB P53779.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 277576.
Family and domain databases
InterPro	IPR008351. JNK_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01772. JNKMAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JNK_SUBDO

Accession

Primary (citable) accession number: Q966Y3
Secondary accession number(s): Q8I1N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)