Reviewed, UniProtKB/Swiss-Prot Q965Q8 (XYLT_CAEEL)

Names and origin

Protein names

Recommended name:
    Xylosyltransferase sqv-6
    EC=2.4.2.26
Alternative name(s):
    Peptide O-xylosyltransferase
    Squashed vulva protein 6

Gene names

Name:	sqv-6
ORF Names:	Y50D4C.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	806 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. Required for vulval morphogenesis and zygotic cytokinesis, suggesting that glycosaminoglycans play a central role in vulval morphogenesis. Ref.1
Catalytic activity	Transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxy group of an acceptor protein substrate. Ref.1
Cofactor	Divalent cations By similarity.
Pathway	Glycan metabolism; chondroitin sulfate biosynthesis. Glycan metabolism; heparan sulfate biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Disruption phenotype	Worms are infertile because of the failure of the progeny of homozygous mutants to initiate cytokinesis and because of the failure to form an extracellular space between the egg and the eggshell. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 14 family. XylT subfamily. Contains 1 WSC domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Developmental protein Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytokinesis Inferred from mutant phenotype. Source: WormBase determination of adult life span Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: InterPro protein xylosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 806	806	Xylosyltransferase sqv-6		PRO_0000191413
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Signal-anchor for type II membrane protein Potential
	Topological domain	33 – 806	774	Lumenal Potential
	Domain	109 – 205	97	WSC
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Glycosylation	325	1	N-linked (GlcNAc...) Potential
	Glycosylation	614	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	655	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	719	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q965Q8-1 [UniParc]. Last modified March 1, 2004. Version 3. Checksum: 6EF779BEA4B570CB	FASTA	806	93,115
	10 20 30 40 50 60 MLFNGTTKYR DYAIVISLFF LLNVYLLYNT AQHTQVGNSK HISSDSGEKT SNPLPSCEIT 70 80 90 100 110 120 DDLAKSAISR AITPSCKAKL QLEACQLKNG TFTINFPENQ CPNHDSRLID QRIGCFLDKK 130 140 150 160 170 180 EARVLTEFEY KLPKSNGKAT CRKHCYKAGF LYFGLEFGHE CFCGNDVSNA TAVDDVECRA 190 200 210 220 230 240 YKCPGNENSE EFCGGFNAVE IFRTGFRSKV NHRKPTYLPP SSDSIKNPVK ILFLLQLNGR 250 260 270 280 290 300 NERQVKRFLK SIYLPHHYYY IHVDARQNYM FSEMQKVADF LDNIHITERR FSTIWGGASL 310 320 330 340 350 360 LQMFLQVIRD SMKIEKFKDW DYIINFSESD FPILPISDFE RLITVNNGKS FLASHGYNTG 370 380 390 400 410 420 KFIQKQGFEY VFSECDNRMF RIGKREFPQN LRIDGGSDWV GIHRNLAEFS ISDEELPRKL 430 440 450 460 470 480 RKTYESILLP LESFYHTLAF NSEFCDDLLM SNLRLTNWYR KQGCRCASLK PIVDWCGCSP 490 500 510 520 530 540 LVFREETMKK FELQKAISKP TYFARKFDSM VDIDSIEAAE MQSISPEKLQ LNHPTYHFAF 550 560 570 580 590 600 ANIFKTGIDE QKLHFESLAN FALKSTETRA KFRKVLRIDA LRAHHNALIE IVMKIETTDG 610 620 630 640 650 660 ATFEFLIHRL SHVNLTENEE KLVEHGYLLR AVSFGTKFEW KEELCREYMG FVTDNDTLHT 670 680 690 700 710 720 RLQWHPTEHV KKVGDKTSPE MIFKYRKGDE LIEQTVVKPY DSVFGGQFDS WNVGKKLSNL 730 740 750 760 770 780 TTCSNFFVDI ISPSSPDDAP PLATLHFPVY TDQNAHCHVD YLRQFFKIAD FCTSGDACKE 790 800 KIWSTSYPDP KSDIFVGYDE DTQTLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Caenorhabditis elegans genes sqv-2 and sqv-6, which are required for vulval morphogenesis, encode glycosaminoglycan galactosyltransferase II and xylosyltransferase." Hwang H.-Y., Olson S.K., Brown J.R., Esko J.D., Horvitz H.R. J. Biol. Chem. 278:11735-11738(2003) [PubMed: 12584198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, DISRUPTION PHENOTYPE.
[2]	"Cloning of Caenorhabditis elegans xylosyltransferase cDNA." Wilson I.B.H. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-614 AND ASN-655, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY241928 mRNA. Translation: AAO85277.1. AJ496235 mRNA. Translation: CAD42732.1. AC025722 Genomic DNA. Translation: AAK68509.3.
RefSeq	NP_503359.2.
UniGene	Cel.1679
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
Genome annotation databases
Ensembl	Y50D4C.4. Caenorhabditis elegans. [Contig view]
GeneID	190099.
KEGG	cel:Y50D4C.4.
NMPDR	fig|6239.3.peg.16656.
Organism-specific databases
WormBase	WBGene00005024. sqv-6.
WormPep	Y50D4C.4. CE35919. [WorfDB]
Phylogenomic databases
OMA	Q965Q8. LNGRNER.
Enzyme and pathway databases
BRENDA	2.4.2.26. 672.
Gene expression databases
ArrayExpress	Q965Q8.
Family and domain databases
InterPro	IPR003406. Glyco_trans_14. IPR002889. WSC_carb_bd. [Graphical view]
PANTHER	PTHR19297. Glyco_trans_14. 1 hit.
Pfam	PF02485. Branch. 1 hit. PF01822. WSC. 1 hit. [Graphical view]
PROSITE	PS51212. WSC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	944650.

Entry information

Entry name

XYLT_CAEEL

Accession

Primary (citable) accession number: Q965Q8
Secondary accession number(s): Q867S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	March 1, 2004
Last modified:	May 5, 2009
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project