Reviewed, UniProtKB/Swiss-Prot Q96581 (C75A4_GENTR)

Names and origin

Protein names

Recommended name:
    Flavonoid 3',5'-hydroxylase
      Short name=F3'5'H
    EC=1.14.13.88
Alternative name(s):
    Cytochrome P450 75A4

Gene names

Name:

CYP75A4

Organism

Gentiana triflora (Clustered gentian)

Taxonomic identifier

55190 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Gentianaceae › Gentianeae › Gentiana

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin By similarity.
Catalytic activity	A flavanone + NADPH + O2 = a 3'-hydroxyflavanone + NADP+ + H2O. A 3'-hydroxyflavanone + NADPH + O2 = a 3',5'-dihydroxyflavanone + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavonoid 3',5'-hydroxylase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Flavonoid 3',5'-hydroxylase		PRO_0000052132
Sites
	Metal binding	453	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96581-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 148259BB35743731	FASTA	516	58,088
	10 20 30 40 50 60 MSPIYTTLTL HLATALFLFF HVQKLVHYLH GKATGHRCRR LPPGPTGWPI LGALPLLGNM 70 80 90 100 110 120 PHVTFANMAK KYGSVMYLKV GSHGLAIAST PDAAKAFLKT LDLNFSNRPP NAGATHLAYN 130 140 150 160 170 180 AQDMVFAHYG PKWKLLRKLS NLHMLGGKAL ENWADVRKTE LGYMLKAMFE SSQNNEPVMI 190 200 210 220 230 240 SEMLTYAMAN MLSQVILSRR VFNKKGAKSN EFKDMVVELM TSAGYFNIGD FIPSIGWMDL 250 260 270 280 290 300 QGIEGGMKRL HKKFDVLLTR LLDDHKRTSQ ERKQKPDFLD FVIANGDNSD GERLNTDNIK 310 320 330 340 350 360 ALLLNLFTAG TDTSSSIIEW ALAELLKNRT LLTRAQDEMD RVIGRDRRLL ESDIPNLPYL 370 380 390 400 410 420 QAICKETFRK HPSTPLNLPR NCIRGHVDVN GYYIPKGTRL NVNIWAIGRD PSVWGDNPNE 430 440 450 460 470 480 FDPERFLYGR NAKIDPRGNH FELIPFGAGR RICAGTRMGI LLVEYILGTL VHSFDWKLGF 490 500 510 SEDELNMDET FGLALQKAVP LAAMVIPRLP LHVYAP

References

[1]

"Molecular and biochemical characterization of three anthocyanin synthetic enzymes from Gentiana triflora." Tanaka Y., Yonekura K., Fukuchi-Mizutani M., Fukui Y., Fujiwara H., Ashikari T., Kusumi T. Plant Cell Physiol. 37:711-716(1996) [PubMed: 8819318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Petal.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D85184 mRNA. Translation: BAA12735.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.88. 10472.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C75A4_GENTR

Accession

Primary (citable) accession number: Q96581

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	March 24, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)