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Protein

Pectinesterase 2.2

Gene

PME2.2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Pectinesterase may play a role in cell wall metabolism during fruit growth and development prior to ripening and may be required for preparing cell walls for softening by polygalacturonase during fruit ripening.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase 1 (PME1.9), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase 2.1 (PME2.1), Pectinesterase, Pectinesterase (PMEU1), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase 2.2 (PME2.2), Pectinesterase, Pectinesterase (LePME1), Pectinesterase 3 (PME3), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase/pectinesterase inhibitor U1 (PMEU1), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase
  2. Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Probable pectate lyase P59 (LAT59), Probable pectate lyase P56 (LAT56), Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Probable pectate lyase P18 (9612), Pectate lyase, Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei312 – 3121SubstrateBy similarity
Binding sitei342 – 3421SubstrateBy similarity
Sitei364 – 3641Transition state stabilizerBy similarity
Active sitei365 – 3651Proton donorPROSITE-ProRule annotation
Active sitei386 – 3861NucleophilePROSITE-ProRule annotation
Binding sitei454 – 4541SubstrateBy similarity
Binding sitei456 – 4561SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 2.2 (EC:3.1.1.11)
Short name:
PE 2.2
Alternative name(s):
Pectin methylesterase 2.2
Gene namesi
Name:PME2.2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 233Sequence analysisPRO_0000023490
Signal peptidei1 – ?Sequence analysis
Chaini234 – 550317Pectinesterase 2.2PRO_0000023491Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence analysis
Disulfide bondi331 ↔ 358By similarity
Disulfide bondi399 ↔ 433By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ96575.
SMRiQ96575. Positions 234-550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ96575.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPQQPLLT KTHKQNSIIS FKILTFVVTL FVALFLVVFL VAPYQFEIKH
60 70 80 90 100
SNLCKTAQDS QLCLSYVSDL MSNEIVTTDS DGLSILMKFL VNYVHQMNNA
110 120 130 140 150
IPVVSKMKNQ INDIRQEGAL TDCLELLDQS VDLVSDSIAA IDKRTHSEHA
160 170 180 190 200
NAQSWLSGVL TNHVTCLDEL DSFTKAMING TNLDELISRA KVALAMLASV
210 220 230 240 250
TTPNDDVLRP GLGKMPSWVS SRDRKLMESS GKDIGANAVV AKDGTGKYRT
260 270 280 290 300
LAEAVAAAPD KSKTRYVIYV KRGIYKENVE VSSRKMKLMI VGDGMHATII
310 320 330 340 350
TGNLNVVDGS TTFHSATLAA VGKGFILQDI CIQNTAGPAK HQAVALRVGA
360 370 380 390 400
DKSVINRCRI DAYQDTLYAH SQRQFYRDSY VTGTIDFIFG NAAVVFQKCK
410 420 430 440 450
LVARKPGKYQ QNMVTAQGRT DPNQATGTSI QFCNIIASSD LEPVLKEFPT
460 470 480 490 500
YLGRPWKKYS RTVVMESYLG GLINPAGWAE WDGDFALKTL YYGEFMNNGP
510 520 530 540 550
GAGTSKRVKW PGYHCITDPA EAMPFTVAKL IQGGSWLRST GVAYVDGLYD
Length:550
Mass (Da):60,425
Last modified:February 1, 1997 - v1
Checksum:iBF95179FAD94ED51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70675 Genomic DNA. Translation: AAB38792.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70675 Genomic DNA. Translation: AAB38792.1.

3D structure databases

ProteinModelPortaliQ96575.
SMRiQ96575. Positions 234-550.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiQ96575.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Fruit-specific pectin methylesterase genomic DNA from Lycopersicon esculentum var. VFNT Cherry."
    Turner L.A., Harriman R.W., Handa A.K.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. VFNT Cherry.

Entry informationi

Entry nameiPME22_SOLLC
AccessioniPrimary (citable) accession number: Q96575
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.