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Q96562 (CHS2_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase 2

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase 2
Gene names
Name:CHS2
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. Substrate preference is feruloyl-CoA = caffeoyl-CoA >> cinnamoyl-CoA.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Induction

Strong in response to inoculation with fungi.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399Chalcone synthase 2
PRO_0000215980

Sites

Active site1661 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q96562 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: B41F54CD647FF22D

FASTA39943,189
        10         20         30         40         50         60 
MAAVRLKEVR MAQRAEGLAT VLAIGTAVPA NCVYQATYPD YYFRVTKSEH LADLKEKFQR 

        70         80         90        100        110        120 
MCDKSMIRKR HMHLTEEILI KNPKICAHME TSLDARHAIA LVEVPKLGQG AAEKAIKEWG 

       130        140        150        160        170        180 
QPLSKITHLV FCTTSGVDMP GADYQLTKLL GLSPTVKRLM MYQQGCFGGA TVLRLAKDIA 

       190        200        210        220        230        240 
ENNRGARVLV VCSEITAMAF RGPCKSHLDS LVGHALFGDG AAAAIIGADP DQLDEQPVFQ 

       250        260        270        280        290        300 
LVSASQTILP ESEGAIDGHL TEAGLTIHLL KDVPGLISEN IEQALEDAFE PLGIHNWNSI 

       310        320        330        340        350        360 
FWIAHPGGPA ILDRVEDRVG LDKKRMRASR EVLSEYGNMS SASVLFVLDV MRKSSAKDGL 

       370        380        390 
ATTGEGKDWG VLFGFGPGLT VETLVLHSVP VPVPTAASA 

« Hide

References

[1]"A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack."
Christensen A.B., Gregersen P.L., Schroeder J., Collinge D.B.
Plant Mol. Biol. 37:849-857(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Pallas / P-02.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09233 mRNA. Translation: CAA70435.1.
PIRT04485.

3D structure databases

ProteinModelPortalQ96562.
SMRQ96562. Positions 6-391.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ96562.

Enzyme and pathway databases

UniPathwayUPA00154.

Gene expression databases

GenevestigatorQ96562.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHS2_HORVU
AccessionPrimary (citable) accession number: Q96562
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: October 16, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways