true1997-11-012024-03-27184ADHX_ARATHCloning of the Arabidopsis and rice formaldehyde dehydrogenase genes: implications for the origin of plant ADH enzymes.Dolferus R.Osterman J.C.Peacock W.J.Dennis E.S.doi:10.1093/genetics/146.3.11311997Genetics1461131-1141NUCLEOTIDE SEQUENCE [GENOMIC DNA]cv. C24Arabidopsis formaldehyde dehydrogenase. Molecular properties of plant class III alcohol dehydrogenase provide further insights into the origins, structure and function of plant class P and liver class I alcohol dehydrogenases.Martinez M.C.Achkor H.Persson B.Fernandez M.R.Shafqat J.Farres J.Joernvall H.Pares X.doi:10.1111/j.1432-1033.1996.00849.x1996Eur. J. Biochem.241849-857NUCLEOTIDE SEQUENCE [MRNA]TISSUE SPECIFICITYCATALYTIC ACTIVITYBIOPHYSICOCHEMICAL PROPERTIEScv. Landsberg erectaFlowerStructural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones.Kotani H.Nakamura Y.Sato S.Kaneko T.Asamizu E.Miyajima N.Tabata S.doi:10.1093/dnares/4.4.2911997DNA Res.4291-300NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]cv. ColumbiaAraport11: a complete reannotation of the Arabidopsis thaliana reference genome.Cheng C.Y.Krishnakumar V.Chan A.P.Thibaud-Nissen F.Schobel S.Town C.D.doi:10.1111/tpj.134152017Plant J.89789-804GENOME REANNOTATIONEmpirical analysis of transcriptional activity in the Arabidopsis genome.Yamada K.Lim J.Dale J.M.Chen H.Shinn P.Palm C.J.Southwick A.M.Wu H.C.Kim C.J.Nguyen M.Pham P.K.Cheuk R.F.Karlin-Newmann G.Liu S.X.Lam B.Sakano H.Wu T.Yu G.Miranda M.Quach H.L.Tripp M.Chang C.H.Lee J.M.Toriumi M.J.Chan M.M.Tang C.C.Onodera C.S.Deng J.M.Akiyama K.Ansari Y.Arakawa T.Banh J.Banno F.Bowser L.Brooks S.Y.Carninci P.Chao Q.Choy N.Enju A.Goldsmith A.D.Gurjal M.Hansen N.F.Hayashizaki Y.Johnson-Hopson C.Hsuan V.W.Iida K.Karnes M.Khan S.Koesema E.Ishida J.Jiang P.X.Jones T.Kawai J.Kamiya A.Meyers C.Nakajima M.Narusaka M.Seki M.Sakurai T.Satou M.Tamse R.Vaysberg M.Wallender E.K.Wong C.Yamamura Y.Yuan S.Shinozaki K.Davis R.W.Theologis A.Ecker J.R.doi:10.1126/science.10883052003Science302842-846NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.Totoki Y.Seki M.Ishida J.Nakajima M.Enju A.Kamiya A.Narusaka M.Shin-i T.Nakagawa M.Sakamoto N.Oishi K.Kohara Y.Kobayashi M.Toyoda A.Sakaki Y.Sakurai T.Iida K.Akiyama K.Satou M.Toyoda T.Konagaya A.Carninci P.Kawai J.Hayashizaki Y.Shinozaki K.2006-07EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]Full-length cDNA from Arabidopsis thaliana.Brover V.V.Troukhan M.E.Alexandrov N.A.Lu Y.-P.Flavell R.B.Feldmann K.A.2002-03EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]The gene encoding glutathione-dependent formaldehyde dehydrogenase/GSNO reductase is responsive to wounding, jasmonic acid and salicylic acid.Diaz M.Achkor H.Titarenko E.Martinez M.C.doi:10.1016/s0014-5793(03)00426-52003FEBS Lett.543136-139INDUCTIONEnhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis.Achkor H.Diaz M.Fernandez M.R.Biosca J.A.Pares X.Martinez M.C.doi:10.1104/pp.103.0222772003Plant Physiol.1322248-2255FUNCTIONCATALYTIC ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESHOMODIMERProteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms.Reumann S.Babujee L.Ma C.Wienkoop S.Siemsen T.Antonicelli G.E.Rasche N.Lueder F.Weckwerth W.Jahn O.doi:10.1105/tpc.107.0509892007Plant Cell193170-3193IDENTIFICATION BY MASS SPECTROMETRYPhosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana.Jones A.M.E.MacLean D.Studholme D.J.Serna-Sanz A.Andreasson E.Rathjen J.P.Peck S.C.doi:10.1016/j.jprot.2009.02.0042009J. Proteomics72439-451IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.Bienvenut W.V.Sumpton D.Martinez A.Lilla S.Espagne C.Meinnel T.Giglione C.doi:10.1074/mcp.m111.0151312012Mol. Cell. Proteomics11M111.015131ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Crystal structure and kinetic behavior of alcohol dehydrogenase III /S-nitrosoglutathione reductase from arabidopsis thaliana.Crotty J.Greving M.Brettschneider S.Weichsel A.Wildner G.F.Vierling E.Montfort W.R.2011-11PDBX-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 2-379 IN COMPLEX WITH NAD AND ZINCHOMODIMER1.40A/B=2-3791.80A/B=2-3791.80A/B=2-3791.95A/B=2-3794HOT5Eukaryotabaseline and differentialalcohol_DH_class_IIIMedium-chain alcohol dehydrogenases, catalytic domainNAD(P)-binding Rossmann-like DomainADH-like_CADH-like_NADH_3ADH_Zn_CSGroES-like_sfNAD(P)-bd_dom_sfadh_III_F_hydeALCOHOL DEHYDROGENASEALCOHOL DEHYDROGENASE CLASS-3ADH_NADH_zinc_NGroES-likeNAD(P)-binding Rossmann-fold domainsADH_ZINCATAlcohol dehydrogenase class-31.1.1.1Alcohol dehydrogenase class-IIIGlutathione-dependent formaldehyde dehydrogenaseFALDHFDHGSH-FDH1.1.1.-S-(hydroxymethyl)glutathione dehydrogenase1.1.1.284ADH2ADHIIIFDH1At5g43940MRH10.4Plays a central role in formaldehyde detoxification.Binds 2 Zn(2+) ions per subunit.kcat is 1351 min(-1) with S-hydroxymethylglutathione as substrate (at pH 8 and 25 degrees Celsius). kcat is 6 min(-1) with farnesol as substrate (at pH 7.5 and 25 degrees Celsius). kcat is 324 min(-1) with cinnamylalcohol as substrate (at pH 7.5 and 25 degrees Celsius). kcat is 126 min(-1) with farnesol as substrate (at pH 10 and 25 degrees Celsius). kcat is 1200 min(-1) with geraniol as substrate (at pH 10 and 25 degrees Celsius). kcat is 1220 min(-1) with cinnamylalcohol as substrate (at pH 10 and 25 degrees Celsius). kcat is 335 min(-1) with 12-Hydroxydodecanoic acid as substrate (at pH 10 and 25 degrees Celsius).1.4 uM for S-(hydroxymethyl)glutathione7 uM for S-hydroxymethylglutathione (at pH 8 and 25 degrees Celsius)7.7 uM for farnesol (at pH 7.5 and 25 degrees Celsius)22000 uM for cinnamylalcohol (at pH 7.5 and 25 degrees Celsius)3 uM for farnesol (at pH 10 and 25 degrees Celsius)800 uM for geraniol (at pH 10 and 25 degrees Celsius)3500 uM for cinnamylalcohol (at pH 10 and 25 degrees Celsius)4700 uM for 12-Hydroxydodecanoic acid (at pH 10 and 25 degrees Celsius)1.22 umol/min/mg enzymeHomodimer.A number of isoforms are produced. According to EST sequences.Ubiquitous.Down-regulated by wounding and activated by salicylic acid (SA).Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.Removed1Alcohol dehydrogenase class-3405682379474849697099102105113177202207226231272295297320322372N-acetylalanineG84S354815232936465463657890931001031071171201211231261281321351381411441461491561571591601621691721731761781871881901982012052172222252362372392422442462482532602652702752832872892912943043063093123163193243273393463483493553573583643713761catalyticsubstrate22002-03-272406993cdce8b66b3b488cf9b79018abe0266d1MATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSATGVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGVNEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLTLGEINKAFDLLHEGTCLRCVLDTSKtruetruetruetruetruetruetruetruetruetruetruetruetrue