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Protein

Cryptochrome-2

Gene

CRY2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Photoreceptor that mediates primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, and regulates other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. Photoexcited cryptochromes interact with signaling partner proteins to alter gene expression at both transcriptional and post-translational levels and, consequently, regulate the corresponding metabolic and developmental programs (PubMed:21841916). Blue-light absorbing flavoprotein that activates reversible flavin photoreduction via an electron transport chain comprising a tryptophan triad (W-321, W-374 and W-397), or via an alternative electron transport that involves small metabolites, including NADPH, NADH, and ATP. The half-life of the activated signaling state is about 16 minutes (PubMed:25428980, PubMed:23398192). Perceives low blue light (LBL) and responds by directly contacting two bHLH transcription factors, PIF4 and PIF5, at chromatin on E-box variant 5'-CA[CT]GTG-3' to promote their activity and stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation and hyponastic growth in low blue light) (PubMed:26724867, PubMed:19558423). In response to blue light, binds to CIB proteins (e.g. BHLH63/CIB1 and BHLH76/CIB5) to activates transcription and floral initiation (PubMed:24130508). Mediates blue light-induced gene expression, floral initiation and hypocotyl elongation through the interaction with SPA1 that prevents formation of SPA1/COP1 complex but stimulates COP1 binding, and thus inhibits COP1-mediated degradation of transcription factors (e.g. CO and HY5) (PubMed:21514160, PubMed:21511872, PubMed:16093319). Promotes flowering time in continuous light (LL) (PubMed:21296763). Involved in shortening the circadian clock period, especially at 27 degrees Celsius, in blue light (BL). Required to maintain clock genes expression rhythm (PubMed:23511208). Triggers nuclear accumulation of ROS in response to blue light illumination (PubMed:26179959). Involved in blue light-dependent stomatal opening, transpiration and inhibition of stem and root growth, probably by regulating abscisic acid (ABA) (PubMed:22147516, PubMed:16093319, PubMed:16703358, PubMed:9482948, PubMed:9565033). Regulates the timing of flowering by promoting the expression of 'FLOWERING LOCUS T' (FT) in vascular bundles. Negatively regulated by 'FLOWERING LOCUS C' (FLC) (PubMed:14605222, PubMed:17259260). General positive regulator of reversible low light-induced chromatin decompaction (PubMed:20935177). Involved in triggering chromatin decondensation during floral transition (PubMed:17470059). Together with phototropins, involved in phototropism regulation by various blue light fluence; blue light attenuates phototropism in high fluence rates (100 µmol.m-2.s-1) but enhances phototropism in low fluence rates (<1.0 µmol.m-2.s-1) (PubMed:12857830). The effect of near-null magnetic field on flowering is altered by changes of blue light cycle and intensity in a CRY1/CRY2-dependent manner (PubMed:26095447). Involved in the strigolactone signaling that regulates hypocotyl growth in response to blue light (PubMed:24126495).1 Publication22 Publications
Confers resistance to turnip crinkle virus (TCV) by preventing COP1-mediated proteasome-mediated degradation of RPP8/HRT, thus promoting its stability in light. Exposure to darkness or blue-light induces degradation of CRY2, and in turn of RPP8/HRT, resulting in susceptibility to TCV.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232FADBy similarity1
Metal bindingi235Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi243Magnesium 1By similarity1
Sitei321Involved in electron transfer from the protein surface to the FAD cofactor1 Publication1
Metal bindingi355Magnesium 1; via tele nitrogenBy similarity1
Binding sitei356FADBy similarity1
Sitei374Involved in electron transfer from the protein surface to the FAD cofactor1 Publication1
Sitei397Involved in electron transfer from the protein surface to the FAD cofactor1 Publication1
Binding sitei406ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi244 – 248FADBy similarity5
Nucleotide bindingi356 – 357ATPBy similarity2
Nucleotide bindingi387 – 389FADBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • blue light photoreceptor activity Source: TAIR
  • FAD binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • chromatin organization Source: TAIR
  • chromatin remodeling Source: TAIR
  • circadian regulation of calcium ion oscillation Source: TAIR
  • circadian rhythm Source: UniProtKB
  • defense response to virus Source: UniProtKB
  • flavin adenine dinucleotide metabolic process Source: UniProtKB
  • long-day photoperiodism, flowering Source: UniProtKB
  • phototropism Source: UniProtKB
  • positive regulation of flower development Source: TAIR
  • positive regulation of reactive oxygen species metabolic process Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of flower development Source: TAIR
  • regulation of leaf morphogenesis Source: UniProtKB
  • regulation of meristem growth Source: TAIR
  • regulation of photoperiodism, flowering Source: UniProtKB
  • response to absence of light Source: UniProtKB
  • response to blue light Source: UniProtKB
  • response to light stimulus Source: UniProtKB
  • response to low fluence blue light stimulus by blue low-fluence system Source: UniProtKB
  • response to strigolactone Source: UniProtKB
  • response to water deprivation Source: TAIR
  • stomatal movement Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Photoreceptor protein, Receptor

Keywords - Biological processi

Plant defense, Sensory transduction

Keywords - Ligandi

ATP-binding, Chromophore, FAD, Flavoprotein, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-395-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cryptochrome-21 Publication
Short name:
Atcry21 Publication
Alternative name(s):
Blue light photoreceptor1 Publication
Protein PHR homolog 11 Publication
Short name:
AtPHH11 Publication
Protein SUPPRESSOR OF elf3 201 Publication
Gene namesi
Name:CRY21 Publication
Synonyms:PHH11 Publication, SEL201 Publication
Ordered Locus Names:At1g04400Imported
ORF Names:F19P19.14Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G04400.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nuclear body Source: UniProtKB
  • nucleus Source: UniProtKB
  • PML body Source: UniProtKB-SubCell
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

The rapid blue light-mediated reversible interaction between CRY2 and BHLH63/CIB1 is used to design an optogenetic control of target proteins or organelles.5 Publications

Disruption phenotypei

Plants show increased root elongation in blue light (PubMed:16703358, PubMed:21511872). Reduced attenuating effect of high fluence rates of blue light in the cry1 cry2 double mutant. Slow rate of curvature at low fluence rates of blue light in cry1 cry2 (PubMed:12857830). The double mutant cry1 cry2 exhibits a reduced impact of near-null magnetic field on flowering in lower blue light intensity and short days (PubMed:26095447). Little detectable phenotype on circadian clock in blue light (BL). The double mutant cry1 cry2 is impaired in blue light signaling, resulting in long-period, lower-amplitude oscillations at 12 and 17 degrees Celsius and completely abolishing rhythms at 27 degrees Celsius (PubMed:23511208). Reduced hyponastic growth (differential growth-driven upward leaf movement) in low blue light fluence (PubMed:19558423). The double mutant cry1 cry2 is hyposensitive to the strigolactone analog GR24 (PubMed:24126495). The mutant cry2 exposed to a background of red light show severely impaired stomatal opening responses to blue light. The double mutant cry1 cry2 has reduced stomatal conductance, transpiration, and photosynthesis, particularly under the high irradiance of full sunlight at midday, associated with elevated abscisic acid levels (PubMed:22147516). Mutation sel20 suppresses the inhibitory effect of continuous light (LL) on the hypocotyl elongation of elf3-1. The double mutant elf3 sel20 exhibits a late-flowering phenotype (PubMed:21296763). Impaired chromatin decondensation during the floral transition and in low light conditions (PubMed:20935177). Increased sensitivity to turnip crinkle virus (TCV) and associated with reduced HRT levels and stability, and characterized by hypersensitive response (HR) symptoms (PubMed:20624951).11 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi321W → A or F: Photochemically inactive in vitro. Undergo robust light-dependent photoreduction in an in vivo context via an alternative electron transport involving small molecule activators including ATP, NADH, and NADPH. 2 Publications1
Mutagenesisi331W → A: Decreased light sensitivity. Enhanced photoreduction in the presence of added ATP. 1 Publication1
Mutagenesisi337G → E: Loss of activity. 1 Publication1
Mutagenesisi374W → A: Photochemically inactive in vitro. Undergo robust light-dependent photoreduction in an in vivo context via an alternative electron transport involving small molecule activators including ATP, NADH, and NADPH. Enhanced photoreduction in the presence of added ATP. Constitutive interaction with SPA1 and BHLH63/CIB1. 2 Publications1
Mutagenesisi374W → F: Photochemically inactive in vitro. Undergo robust light-dependent photoreduction in an in vivo context via an alternative electron transport involving small molecule activators including ATP, NADH, and NADPH. Enhanced photoreduction in the presence of added ATP. 2 Publications1
Mutagenesisi376W → A: Decreased light sensitivity. Enhanced photoreduction in the presence of added ATP. 1 Publication1
Mutagenesisi377G → R: Constitutive light response. 1 Publication1
Mutagenesisi387D → A: Impaired FAD-binding leading to impaired blue light-mediated inhibition of hypocotyl elongation and loss of blue light-induced degradation. Disturbed BHLH63/CIB1 and SPA1 interactions. 2 Publications1
Mutagenesisi397W → A: Photochemically inactive in vitro. Undergo robust light-dependent photoreduction in an in vivo context via an alternative electron transport involving small molecule activators including ATP, NADH, and NADPH. 2 Publications1
Mutagenesisi397W → F: Photochemically inactive both in vitro and in vivo. 2 Publications1
Mutagenesisi399Y → A or F: Impaired ATP-mediated enhanced photoreduction and decreased affinity for ATP. 1 Publication1
Mutagenesisi541K → R: Impaired nuclear importation leading to reduced phosphorylation, physiological activities, and degradation in response to blue light. Forms protein bodies (photobodies) in both the nucleus and cytosol in response to blue light. 1 Publication1
Mutagenesisi554 – 555KK → RR: Impaired nuclear importation leading to reduced phosphorylation, physiological activities, and degradation in response to blue light. Forms protein bodies (photobodies) in both the nucleus and cytosol in response to blue light. 1 Publication2
Mutagenesisi570 – 575SSSSSS → AAAAAA: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with A-580, A-582, A-584, A-587, 598-A-A-599 and A-605. 1 Publication6
Mutagenesisi570 – 575SSSSSS → DDDDDD: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with D-580, D-582, D-584, D-587, 598-D-D-599 and D-605. 1 Publication6
Mutagenesisi580S → A: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-A--A-573, A-582, A-584, A-587, 598-A-A-599 and A-605. 1 Publication1
Mutagenesisi580S → D: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-D--D-573, D-582, D-584, D-587, 598-D-D-599 and D-605. 1 Publication1
Mutagenesisi582S → A: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-A--A-573, A-580, A-584, A-587, 598-A-A-599 and A-605. 1 Publication1
Mutagenesisi582S → D: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-D--D-573, D-580, D-584, D-587, 598-D-D-599 and D-605. 1 Publication1
Mutagenesisi584S → A: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-A--A-573, A-580, A-582, A-587, 598-A-A-599 and A-605. 1 Publication1
Mutagenesisi584S → D: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-D--D-573, D-580, D-582, D-587, 598-D-D-599 and D-605. 1 Publication1
Mutagenesisi587S → A: Impaired regulation of hypocotyl growth in blue light. Phosphorylated by CK1 proteins CK1.3 and CK1.4. Reduced phosphorylation by CK1 proteins CK1.3 and CK1.4; when associated with A-603. Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-A--A-573, A-580, A-582, A-584, 598-A-A-599 and A-605. 2 Publications1
Mutagenesisi587S → D: Constitutive regulation of hypocotyl growth in blue light. Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-D--D-573, D-580, D-582, D-584, 598-D-D-599 and D-605. 2 Publications1
Mutagenesisi598 – 599SS → AA: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-A--A-573, A-580, A-582, A-584, A-587 and A-605. 1 Publication2
Mutagenesisi598 – 599SS → DD: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-D--D-573, D-580, D-582, D-584, D-587 and D-605. 1 Publication2
Mutagenesisi603T → A: Impaired regulation of hypocotyl growth in blue light. Phosphorylated by CK1 proteins CK1.3 and CK1.4. Reduced phosphorylation by CK1 proteins CK1.3 and CK1.4; when associated with A-587. 1 Publication1
Mutagenesisi603T → D: Constitutive regulation of hypocotyl growth in blue light. 1 Publication1
Mutagenesisi605S → A: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-A--A-573, A-580, A-582, A-584, A-587 and 598-A-A-599. 1 Publication1
Mutagenesisi605S → D: Reduced blue light-mediated phosphorylation and impaired blue light-dependent proteolysis and hypocotyl inhibition response; when associated with 570-D--D-573, D-580, D-582, D-584, D-587 and 598-D-D-599. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851221 – 612Cryptochrome-2Add BLAST612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei587Phosphoserine; by CK11 Publication1
Modified residuei598Phosphoserine1 Publication1
Modified residuei599Phosphoserine1 Publication1
Modified residuei603Phosphothreonine; by CK11 Publication1
Modified residuei605Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by CK1.3 and CK1.4; in response to blue light. Required for degradation (PubMed:12066190, PubMed:17438275, PubMed:17965271, PubMed:9651577, PubMed:25792146, PubMed:23897926). Adopts an open conformation when phosphorylated upon photoexcitation and thus interacts with signaling partner proteins (PubMed:21841916). Not autophosphorylated, even in complex with FAD cofactor (PubMed:17073458).1 Publication7 Publications
Ubiquitinated; in response to blue light.2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ96524.

PTM databases

iPTMnetiQ96524.

Expressioni

Tissue specificityi

Mostly expressed in the shoot meristems and root tips, and, to a lower extent, in the cotyledons, hypocotyls, and roots.1 Publication

Inductioni

Daily oscillation of protein abundance in plants grown in short days (SD) but not in long days (LD) (PubMed:12578985). Expression levels display circadian oscillations under constant conditions, with a low amplitude and a late phase, with maximal expression around the end of the light phase. Repressed by light (PubMed:11743105). In response to blue light and darkness, phosphorylated, ubiquitinated, and subsequently degraded (at protein level) in a SPA proteins-dependent manner (PubMed:20624951, PubMed:25792146, PubMed:22739826, PubMed:22311776). Transcripts levels oscillate weakly and proportionally to temperature, but protein levels are stable (PubMed:23511208). Accumulates in response to low blue light (LBL) and in low light (PubMed:26724867, PubMed:20935177).9 Publications

Gene expression databases

ExpressionAtlasiQ96524. baseline and differential.
GenevisibleiQ96524. AT.

Interactioni

Subunit structurei

Homodimer (PubMed:11089975, PubMed:11509693, PubMed:17438275). Interacts with COP1 and PHYB in the nucleus (PubMed:11089975, PubMed:11509693, PubMed:17438275, PubMed:20624951). Binds reversibly to CIBs proteins such as BHLH63/CIB1, BHLH78/CIB2, BHLH74/CIB4 and BHLH76/CIB5 after blue light illumination to stimulate their transcription factor activities (PubMed:18988809, PubMed:22139370, PubMed:24130508, PubMed:24780222). Interacts with PIF4 and PIF5 in the nucleus in response to low blue light (LBL) (PubMed:26724867). Binds to SPA1 in response to blue light, this interaction prevents SPA1/COP1 complex formation but stimulates interaction with COP1, and thus avoid COP1-dependent degradation of the transcription factors CO and HY5 by the proteasome and promotes hypocotyl elongation and floral initiation (PubMed:21514160, PubMed:22139370, PubMed:21511872, PubMed:22739826). Binding to ATP mediates conformational changes which facilitate flavin binding (PubMed:17073458).13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-531555,EBI-531555
COP1P432542EBI-531555,EBI-301649
PHYBP147133EBI-531555,EBI-300727

Protein-protein interaction databases

BioGridi24764. 10 interactors.
DIPiDIP-33589N.
IntActiQ96524. 2 interactors.
MINTiMINT-207946.
STRINGi3702.AT1G04400.1.

Structurei

3D structure databases

ProteinModelPortaliQ96524.
SMRiQ96524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 134Photolyase/cryptochrome alpha/betaSequence analysisAdd BLAST130

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 485CNT2, binds chromophores to sense blue light and mediate CRY dimerization1 PublicationAdd BLAST485
Regioni486 – 612CCT2/CCE2, mediates blue light signaling1 Publication1 PublicationAdd BLAST127

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi541 – 555Nuclear localization signalPROSITE-ProRule annotationAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 27Poly-AlaSequence analysis6
Compositional biasi546 – 550Poly-GluSequence analysis5
Compositional biasi566 – 587Ser-richPROSITE-ProRule annotationAdd BLAST22

Domaini

The NC80 domain (486-565) contains a major active site responsible for the signal transduction processes regulating both hypocotyl inhibition and floral promotion. The C-terminal tail (564-612) is not required for physiological activity of the protein.

Sequence similaritiesi

Belongs to the DNA photolyase class-1 family.Curated
Contains 1 photolyase/cryptochrome alpha/beta domain.Sequence analysis

Phylogenomic databases

eggNOGiKOG0133. Eukaryota.
COG0415. LUCA.
InParanoidiQ96524.
KOiK12119.
OMAiIIGIWID.
OrthoDBiEOG0936047F.
PhylomeDBiQ96524.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR002081. Cryptochrome/DNA_photolyase_1.
IPR014134. Cryptochrome_pln.
IPR018394. DNA_photolyase_1_CS_C.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
PRINTSiPR00147. DNAPHOTLYASE.
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsiTIGR02766. crypt_chrom_pln. 1 hit.
PROSITEiPS00394. DNA_PHOTOLYASES_1_1. 1 hit.
PS00691. DNA_PHOTOLYASES_1_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMDKKTIVW FRRDLRIEDN PALAAAAHEG SVFPVFIWCP EEEGQFYPGR
60 70 80 90 100
ASRWWMKQSL AHLSQSLKAL GSDLTLIKTH NTISAILDCI RVTGATKVVF
110 120 130 140 150
NHLYDPVSLV RDHTVKEKLV ERGISVQSYN GDLLYEPWEI YCEKGKPFTS
160 170 180 190 200
FNSYWKKCLD MSIESVMLPP PWRLMPITAA AEAIWACSIE ELGLENEAEK
210 220 230 240 250
PSNALLTRAW SPGWSNADKL LNEFIEKQLI DYAKNSKKVV GNSTSLLSPY
260 270 280 290 300
LHFGEISVRH VFQCARMKQI IWARDKNSEG EESADLFLRG IGLREYSRYI
310 320 330 340 350
CFNFPFTHEQ SLLSHLRFFP WDADVDKFKA WRQGRTGYPL VDAGMRELWA
360 370 380 390 400
TGWMHNRIRV IVSSFAVKFL LLPWKWGMKY FWDTLLDADL ECDILGWQYI
410 420 430 440 450
SGSIPDGHEL DRLDNPALQG AKYDPEGEYI RQWLPELARL PTEWIHHPWD
460 470 480 490 500
APLTVLKASG VELGTNYAKP IVDIDTAREL LAKAISRTRE AQIMIGAAPD
510 520 530 540 550
EIVADSFEAL GANTIKEPGL CPSVSSNDQQ VPSAVRYNGS KRVKPEEEEE
560 570 580 590 600
RDMKKSRGFD ERELFSTAES SSSSSVFFVS QSCSLASEGK NLEGIQDSSD
610
QITTSLGKNG CK
Length:612
Mass (Da):69,457
Last modified:December 15, 1998 - v2
Checksum:i082E311301465904
GO

Sequence cautioni

The sequence AAT80593 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80594 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80595 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80596 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80597 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80598 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80599 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80600 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80601 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80602 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80603 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80604 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80605 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80606 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80607 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80608 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80609 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80610 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80611 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80612 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80613 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80614 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80615 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80616 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80617 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80618 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80619 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80620 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80621 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80622 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT80623 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD94467 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78K → Q in AAB04996 (PubMed:9003312).Curated1
Sequence conflicti78K → Q in AAB04997 (PubMed:9003312).Curated1
Sequence conflicti95A → P in AAB04996 (PubMed:9003312).Curated1
Sequence conflicti95A → P in AAB04997 (PubMed:9003312).Curated1
Sequence conflicti188S → L in AAL16379 (PubMed:11726930).Curated1
Sequence conflicti366A → G in AAB04996 (PubMed:9003312).Curated1
Sequence conflicti534A → V in AAB04996 (PubMed:9003312).Curated1
Sequence conflicti534A → V in AAB04997 (PubMed:9003312).Curated1
Sequence conflicti590K → E in CAA67508 (PubMed:9003312).Curated1
Sequence conflicti612K → Q in CAA67508 (PubMed:9003312).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti83I → V in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti127Q → S in strain: cv. Bu-0, cv. Da(1)-12, cv. Di-G, cv. Landsberg erecta, cv. Le-0, cv. Lip-0, cv. Mrk-0, cv. Stw-0 and cv. Ta-0. 1 Publication1
Natural varianti326D → E in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti367V → M in strain: cv. Cvi-0. 1 Publication1
Natural varianti476T → I in strain: cv. Cvi-0. 1 Publication1
Natural varianti482A → G in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti498A → S in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti507F → L in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti511G → E in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti543V → L in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1
Natural varianti611C → Y in strain: cv. Chi-1, cv. Co-1, cv. Kon, cv. PHW-1 and cv. Sha. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62549 mRNA. Translation: AAB04996.1.
U62550 mRNA. Translation: AAB04997.1.
X99061 Genomic DNA. Translation: CAA67508.1.
U43397 mRNA. Translation: AAD09837.1.
AY057440 mRNA. Translation: AAL16377.1.
AY057441 Genomic DNA. Translation: AAL16378.1.
AY057442 Genomic DNA. Translation: AAL16379.1.
EU351967 Genomic DNA. Translation: ABY77601.1.
EU351968 Genomic DNA. Translation: ABY77602.1.
EU351969 Genomic DNA. Translation: ABY77603.1.
EU351970 Genomic DNA. Translation: ABY77604.1.
EU351971 Genomic DNA. Translation: ABY77605.1.
EU351972 Genomic DNA. Translation: ABY77606.1.
EU351973 Genomic DNA. Translation: ABY77607.1.
EU351974 Genomic DNA. Translation: ABY77608.1.
EU351975 Genomic DNA. Translation: ABY77609.1.
EU351976 Genomic DNA. Translation: ABY77610.1.
EU351977 Genomic DNA. Translation: ABY77611.1.
EU351978 Genomic DNA. Translation: ABY77612.1.
EU351979 Genomic DNA. Translation: ABY77613.1.
EU351980 Genomic DNA. Translation: ABY77614.1.
EU351981 Genomic DNA. Translation: ABY77615.1.
EU351982 Genomic DNA. Translation: ABY77616.1.
EU351983 Genomic DNA. Translation: ABY77617.1.
EU351984 Genomic DNA. Translation: ABY77618.1.
EU351985 Genomic DNA. Translation: ABY77619.1.
EU351986 Genomic DNA. Translation: ABY77620.1.
EU351987 Genomic DNA. Translation: ABY77621.1.
EU351988 Genomic DNA. Translation: ABY77622.1.
EU351989 Genomic DNA. Translation: ABY77623.1.
EU351990 Genomic DNA. Translation: ABY77624.1.
EU351991 Genomic DNA. Translation: ABY77625.1.
EU351993 Genomic DNA. Translation: ABY77627.1.
EU351992 Genomic DNA. Translation: ABY77626.1.
EU351994 Genomic DNA. Translation: ABY77628.1.
EU351995 Genomic DNA. Translation: ABY77629.1.
EU351996 Genomic DNA. Translation: ABY77630.1.
EU351997 Genomic DNA. Translation: ABY77631.1.
EU351998 Genomic DNA. Translation: ABY77632.1.
AC000104 Genomic DNA. Translation: AAB70435.1.
CP002684 Genomic DNA. Translation: AEE27692.1.
CP002684 Genomic DNA. Translation: AEE27693.1.
BT008576 mRNA. Translation: AAP40403.1.
BT008648 mRNA. Translation: AAP40463.1.
AY576241 Genomic DNA. Translation: AAT80593.1. Different initiation.
AY576242 Genomic DNA. Translation: AAT80594.1. Different initiation.
AY576243 Genomic DNA. Translation: AAT80595.1. Different initiation.
AY576244 Genomic DNA. Translation: AAT80596.1. Different initiation.
AY576245 Genomic DNA. Translation: AAT80597.1. Different initiation.
AY576246 Genomic DNA. Translation: AAT80598.1. Different initiation.
AY576247 Genomic DNA. Translation: AAT80599.1. Different initiation.
AY576248 Genomic DNA. Translation: AAT80600.1. Different initiation.
AY576249 Genomic DNA. Translation: AAT80601.1. Different initiation.
AY576250 Genomic DNA. Translation: AAT80602.1. Different initiation.
AY576251 Genomic DNA. Translation: AAT80603.1. Different initiation.
AY576252 Genomic DNA. Translation: AAT80604.1. Different initiation.
AY576253 Genomic DNA. Translation: AAT80605.1. Different initiation.
AY576254 Genomic DNA. Translation: AAT80606.1. Different initiation.
AY576255 Genomic DNA. Translation: AAT80607.1. Different initiation.
AY576256 Genomic DNA. Translation: AAT80608.1. Different initiation.
AY576257 Genomic DNA. Translation: AAT80609.1. Different initiation.
AY576258 Genomic DNA. Translation: AAT80610.1. Different initiation.
AY576259 Genomic DNA. Translation: AAT80611.1. Different initiation.
AY576260 Genomic DNA. Translation: AAT80612.1. Different initiation.
AY576261 Genomic DNA. Translation: AAT80613.1. Different initiation.
AY576262 Genomic DNA. Translation: AAT80614.1. Different initiation.
AY576263 Genomic DNA. Translation: AAT80615.1. Different initiation.
AY576264 Genomic DNA. Translation: AAT80616.1. Different initiation.
AY576265 Genomic DNA. Translation: AAT80617.1. Different initiation.
AY576266 Genomic DNA. Translation: AAT80618.1. Different initiation.
AY576267 Genomic DNA. Translation: AAT80619.1. Different initiation.
AY576268 Genomic DNA. Translation: AAT80620.1. Different initiation.
AY576269 Genomic DNA. Translation: AAT80621.1. Different initiation.
AY576270 Genomic DNA. Translation: AAT80622.1. Different initiation.
AY576271 Genomic DNA. Translation: AAT80623.1. Different initiation.
AK220946 mRNA. Translation: BAD94467.1. Different initiation.
PIRiA86176.
S71221.
RefSeqiNP_171935.1. NM_100320.4.
NP_849588.1. NM_179257.2.
UniGeneiAt.21976.

Genome annotation databases

EnsemblPlantsiAT1G04400.1; AT1G04400.1; AT1G04400.
AT1G04400.2; AT1G04400.2; AT1G04400.
GeneIDi839529.
GrameneiAT1G04400.1; AT1G04400.1; AT1G04400.
AT1G04400.2; AT1G04400.2; AT1G04400.
KEGGiath:AT1G04400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62549 mRNA. Translation: AAB04996.1.
U62550 mRNA. Translation: AAB04997.1.
X99061 Genomic DNA. Translation: CAA67508.1.
U43397 mRNA. Translation: AAD09837.1.
AY057440 mRNA. Translation: AAL16377.1.
AY057441 Genomic DNA. Translation: AAL16378.1.
AY057442 Genomic DNA. Translation: AAL16379.1.
EU351967 Genomic DNA. Translation: ABY77601.1.
EU351968 Genomic DNA. Translation: ABY77602.1.
EU351969 Genomic DNA. Translation: ABY77603.1.
EU351970 Genomic DNA. Translation: ABY77604.1.
EU351971 Genomic DNA. Translation: ABY77605.1.
EU351972 Genomic DNA. Translation: ABY77606.1.
EU351973 Genomic DNA. Translation: ABY77607.1.
EU351974 Genomic DNA. Translation: ABY77608.1.
EU351975 Genomic DNA. Translation: ABY77609.1.
EU351976 Genomic DNA. Translation: ABY77610.1.
EU351977 Genomic DNA. Translation: ABY77611.1.
EU351978 Genomic DNA. Translation: ABY77612.1.
EU351979 Genomic DNA. Translation: ABY77613.1.
EU351980 Genomic DNA. Translation: ABY77614.1.
EU351981 Genomic DNA. Translation: ABY77615.1.
EU351982 Genomic DNA. Translation: ABY77616.1.
EU351983 Genomic DNA. Translation: ABY77617.1.
EU351984 Genomic DNA. Translation: ABY77618.1.
EU351985 Genomic DNA. Translation: ABY77619.1.
EU351986 Genomic DNA. Translation: ABY77620.1.
EU351987 Genomic DNA. Translation: ABY77621.1.
EU351988 Genomic DNA. Translation: ABY77622.1.
EU351989 Genomic DNA. Translation: ABY77623.1.
EU351990 Genomic DNA. Translation: ABY77624.1.
EU351991 Genomic DNA. Translation: ABY77625.1.
EU351993 Genomic DNA. Translation: ABY77627.1.
EU351992 Genomic DNA. Translation: ABY77626.1.
EU351994 Genomic DNA. Translation: ABY77628.1.
EU351995 Genomic DNA. Translation: ABY77629.1.
EU351996 Genomic DNA. Translation: ABY77630.1.
EU351997 Genomic DNA. Translation: ABY77631.1.
EU351998 Genomic DNA. Translation: ABY77632.1.
AC000104 Genomic DNA. Translation: AAB70435.1.
CP002684 Genomic DNA. Translation: AEE27692.1.
CP002684 Genomic DNA. Translation: AEE27693.1.
BT008576 mRNA. Translation: AAP40403.1.
BT008648 mRNA. Translation: AAP40463.1.
AY576241 Genomic DNA. Translation: AAT80593.1. Different initiation.
AY576242 Genomic DNA. Translation: AAT80594.1. Different initiation.
AY576243 Genomic DNA. Translation: AAT80595.1. Different initiation.
AY576244 Genomic DNA. Translation: AAT80596.1. Different initiation.
AY576245 Genomic DNA. Translation: AAT80597.1. Different initiation.
AY576246 Genomic DNA. Translation: AAT80598.1. Different initiation.
AY576247 Genomic DNA. Translation: AAT80599.1. Different initiation.
AY576248 Genomic DNA. Translation: AAT80600.1. Different initiation.
AY576249 Genomic DNA. Translation: AAT80601.1. Different initiation.
AY576250 Genomic DNA. Translation: AAT80602.1. Different initiation.
AY576251 Genomic DNA. Translation: AAT80603.1. Different initiation.
AY576252 Genomic DNA. Translation: AAT80604.1. Different initiation.
AY576253 Genomic DNA. Translation: AAT80605.1. Different initiation.
AY576254 Genomic DNA. Translation: AAT80606.1. Different initiation.
AY576255 Genomic DNA. Translation: AAT80607.1. Different initiation.
AY576256 Genomic DNA. Translation: AAT80608.1. Different initiation.
AY576257 Genomic DNA. Translation: AAT80609.1. Different initiation.
AY576258 Genomic DNA. Translation: AAT80610.1. Different initiation.
AY576259 Genomic DNA. Translation: AAT80611.1. Different initiation.
AY576260 Genomic DNA. Translation: AAT80612.1. Different initiation.
AY576261 Genomic DNA. Translation: AAT80613.1. Different initiation.
AY576262 Genomic DNA. Translation: AAT80614.1. Different initiation.
AY576263 Genomic DNA. Translation: AAT80615.1. Different initiation.
AY576264 Genomic DNA. Translation: AAT80616.1. Different initiation.
AY576265 Genomic DNA. Translation: AAT80617.1. Different initiation.
AY576266 Genomic DNA. Translation: AAT80618.1. Different initiation.
AY576267 Genomic DNA. Translation: AAT80619.1. Different initiation.
AY576268 Genomic DNA. Translation: AAT80620.1. Different initiation.
AY576269 Genomic DNA. Translation: AAT80621.1. Different initiation.
AY576270 Genomic DNA. Translation: AAT80622.1. Different initiation.
AY576271 Genomic DNA. Translation: AAT80623.1. Different initiation.
AK220946 mRNA. Translation: BAD94467.1. Different initiation.
PIRiA86176.
S71221.
RefSeqiNP_171935.1. NM_100320.4.
NP_849588.1. NM_179257.2.
UniGeneiAt.21976.

3D structure databases

ProteinModelPortaliQ96524.
SMRiQ96524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24764. 10 interactors.
DIPiDIP-33589N.
IntActiQ96524. 2 interactors.
MINTiMINT-207946.
STRINGi3702.AT1G04400.1.

PTM databases

iPTMnetiQ96524.

Proteomic databases

PaxDbiQ96524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G04400.1; AT1G04400.1; AT1G04400.
AT1G04400.2; AT1G04400.2; AT1G04400.
GeneIDi839529.
GrameneiAT1G04400.1; AT1G04400.1; AT1G04400.
AT1G04400.2; AT1G04400.2; AT1G04400.
KEGGiath:AT1G04400.

Organism-specific databases

TAIRiAT1G04400.

Phylogenomic databases

eggNOGiKOG0133. Eukaryota.
COG0415. LUCA.
InParanoidiQ96524.
KOiK12119.
OMAiIIGIWID.
OrthoDBiEOG0936047F.
PhylomeDBiQ96524.

Enzyme and pathway databases

BioCyciARA:GQT-395-MONOMER.

Miscellaneous databases

PROiQ96524.

Gene expression databases

ExpressionAtlasiQ96524. baseline and differential.
GenevisibleiQ96524. AT.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR002081. Cryptochrome/DNA_photolyase_1.
IPR014134. Cryptochrome_pln.
IPR018394. DNA_photolyase_1_CS_C.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
PRINTSiPR00147. DNAPHOTLYASE.
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsiTIGR02766. crypt_chrom_pln. 1 hit.
PROSITEiPS00394. DNA_PHOTOLYASES_1_1. 1 hit.
PS00691. DNA_PHOTOLYASES_1_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRY2_ARATH
AccessioniPrimary (citable) accession number: Q96524
Secondary accession number(s): B0LQ23
, B0LQ24, B0LQ25, B0LQ29, Q42549, Q42603, Q42604, Q56ZL8, Q696X6, Q696X8, Q696Z7, Q697A2, Q8VWL9, Q8VZY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylation of the C-terminal tail and resulting derepression of NC80 domain may both depend on homodimerization.

Caution

Was originally thought to be a DNA photolyase.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.