Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxidase 12

Gene

PER12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Exhibits a Ca2+-pectate binding affinity which could be interpreted in vivo as a specificity to interact with the pectic structure of the cell wall.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei80 – 801Transition state stabilizerPROSITE-ProRule annotation
Active sitei84 – 841Proton acceptorPROSITE-ProRule annotation
Metal bindingi85 – 851Calcium 1PROSITE-ProRule annotation
Metal bindingi88 – 881Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi90 – 901Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi92 – 921Calcium 1PROSITE-ProRule annotation
Metal bindingi94 – 941Calcium 1PROSITE-ProRule annotation
Binding sitei183 – 1831Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi213 – 2131Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi214 – 2141Calcium 2PROSITE-ProRule annotation
Metal bindingi259 – 2591Calcium 2PROSITE-ProRule annotation
Metal bindingi262 – 2621Calcium 2PROSITE-ProRule annotation
Metal bindingi267 – 2671Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G71695-MONOMER.

Protein family/group databases

PeroxiBasei93. AtPrx12.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 12 (EC:1.11.1.7)
Short name:
Atperox P12
Alternative name(s):
ATP4a
PRXR6
Gene namesi
Name:PER12
Synonyms:P12
Ordered Locus Names:At1g71695
ORF Names:F14O23.6, F26A9.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G71695.

Subcellular locationi

  • Secreted Curated
  • Vacuole Curated

  • Note: Carboxy-terminal extension appears to target the protein to vacuoles.

GO - Cellular componenti

  • cell wall Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
  • membrane Source: TAIR
  • plant-type cell wall Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 358327Peroxidase 12PRO_0000023678Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 134PROSITE-ProRule annotation
Disulfide bondi86 ↔ 91PROSITE-ProRule annotation
Disulfide bondi140 ↔ 335PROSITE-ProRule annotation
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence analysis
Disulfide bondi220 ↔ 247PROSITE-ProRule annotation
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96520.
PRIDEiQ96520.

Expressioni

Tissue specificityi

Expressed in roots and leaves.

Developmental stagei

Expressed in the first stage of developing seeds.1 Publication

Inductioni

Induced either by incompatible fungal pathogen attack, or by methyl jasmonate, a plant defense-related signaling molecule.1 Publication

Gene expression databases

GenevisibleiQ96520. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G71695.1.

Structurei

3D structure databases

ProteinModelPortaliQ96520.
SMRiQ96520. Positions 37-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHDZ. Eukaryota.
ENOG410ZMT4. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ96520.
KOiK00430.
OMAiCSARNTQ.
OrthoDBiEOG09360F5G.
PhylomeDBiQ96520.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96520-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKAYSTRVL TFLILISLMA VTLNLFPTVE AKKRSRDAPI VKGLSWNFYQ
60 70 80 90 100
KACPKVENII RKELKKVFKR DIGLAAAILR IHFHDCFVQG CEASVLLAGS
110 120 130 140 150
ASGPGEQSSI PNLTLRQQAF VVINNLRALV QKKCGQVVSC SDILALAARD
160 170 180 190 200
SVVLSGGPDY AVPLGRRDSL AFASQETTLN NLPPPFFNAS QLIADFANRN
210 220 230 240 250
LNITDLVALS GGHTIGIAHC PSFTDRLYPN QDPTMNQFFA NSLKRTCPTA
260 270 280 290 300
NSSNTQVNDI RSPDVFDNKY YVDLMNRQGL FTSDQDLFVD KRTRGIVESF
310 320 330 340 350
AIDQQLFFDY FTVAMIKMGQ MSVLTGTQGE IRSNCSARNT QSFMSVLEEG

IEEAISMI
Length:358
Mass (Da):39,559
Last modified:February 1, 1997 - v1
Checksum:iB8F18C56CFCB4CB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti257 – 2571V → G in CAA66962 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98318 mRNA. Translation: CAA66962.1.
X98773 mRNA. Translation: CAA67309.1.
AC012654 Genomic DNA. Translation: AAF43221.1.
AC016163 Genomic DNA. Translation: AAG51834.1.
CP002684 Genomic DNA. Translation: AEE35217.1.
AF334732 mRNA. Translation: AAG50110.1.
BT000715 mRNA. Translation: AAN31858.1.
AY087964 mRNA. Translation: AAM65511.1.
PIRiA96739.
RefSeqiNP_177313.1. NM_105826.3.
UniGeneiAt.67041.
At.94.

Genome annotation databases

EnsemblPlantsiAT1G71695.1; AT1G71695.1; AT1G71695.
GeneIDi843498.
GrameneiAT1G71695.1; AT1G71695.1; AT1G71695.
KEGGiath:AT1G71695.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98318 mRNA. Translation: CAA66962.1.
X98773 mRNA. Translation: CAA67309.1.
AC012654 Genomic DNA. Translation: AAF43221.1.
AC016163 Genomic DNA. Translation: AAG51834.1.
CP002684 Genomic DNA. Translation: AEE35217.1.
AF334732 mRNA. Translation: AAG50110.1.
BT000715 mRNA. Translation: AAN31858.1.
AY087964 mRNA. Translation: AAM65511.1.
PIRiA96739.
RefSeqiNP_177313.1. NM_105826.3.
UniGeneiAt.67041.
At.94.

3D structure databases

ProteinModelPortaliQ96520.
SMRiQ96520. Positions 37-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G71695.1.

Protein family/group databases

PeroxiBasei93. AtPrx12.

Proteomic databases

PaxDbiQ96520.
PRIDEiQ96520.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G71695.1; AT1G71695.1; AT1G71695.
GeneIDi843498.
GrameneiAT1G71695.1; AT1G71695.1; AT1G71695.
KEGGiath:AT1G71695.

Organism-specific databases

TAIRiAT1G71695.

Phylogenomic databases

eggNOGiENOG410IHDZ. Eukaryota.
ENOG410ZMT4. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ96520.
KOiK00430.
OMAiCSARNTQ.
OrthoDBiEOG09360F5G.
PhylomeDBiQ96520.

Enzyme and pathway databases

BioCyciARA:AT1G71695-MONOMER.

Miscellaneous databases

PROiQ96520.

Gene expression databases

GenevisibleiQ96520. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER12_ARATH
AccessioniPrimary (citable) accession number: Q96520
Secondary accession number(s): Q43734
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.