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Protein

Peroxidase 35

Gene

PER35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei63 – 631Transition state stabilizerPROSITE-ProRule annotation
Active sitei67 – 671Proton acceptorPROSITE-ProRule annotation
Metal bindingi68 – 681Calcium 1PROSITE-ProRule annotation
Metal bindingi71 – 711Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi73 – 731Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi75 – 751Calcium 1PROSITE-ProRule annotation
Metal bindingi77 – 771Calcium 1PROSITE-ProRule annotation
Binding sitei167 – 1671Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi197 – 1971Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi198 – 1981Calcium 2PROSITE-ProRule annotation
Metal bindingi249 – 2491Calcium 2PROSITE-ProRule annotation
Metal bindingi252 – 2521Calcium 2PROSITE-ProRule annotation
Metal bindingi257 – 2571Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G49960-MONOMER.

Protein family/group databases

PeroxiBasei201. AtPrx35.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 35 (EC:1.11.1.7)
Short name:
Atperox P35
Alternative name(s):
ATP21a
Gene namesi
Name:PER35
Synonyms:P35
Ordered Locus Names:At3g49960
ORF Names:F3A4.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G49960.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 329304Peroxidase 35PRO_0000023701Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 119PROSITE-ProRule annotation
Disulfide bondi69 ↔ 74PROSITE-ProRule annotation
Disulfide bondi125 ↔ 325PROSITE-ProRule annotation
Disulfide bondi204 ↔ 236PROSITE-ProRule annotation
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96510.
PRIDEiQ96510.

Expressioni

Tissue specificityi

Slightly expressed in roots.

Inductioni

Up-regulated transiently by a cold treatment.1 Publication

Gene expression databases

ExpressionAtlasiQ96510. baseline and differential.
GenevisibleiQ96510. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G49960.1.

Structurei

3D structure databases

ProteinModelPortaliQ96510.
SMRiQ96510. Positions 27-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJ72. Eukaryota.
ENOG4111FDS. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ96510.
KOiK00430.
OMAiKIFHANF.
OrthoDBiEOG09360FB9.
PhylomeDBiQ96510.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFDIVLLI GLCLIISVFP DTTTAQLSRG FYSKTCPNVE QIVRNAVQKK
60 70 80 90 100
IKKTFVAVPA TLRLFFHDCF VNGCDASVMI QSTPKNKAEK DHPDNISLAG
110 120 130 140 150
DGFDVVIQAK KALDSNPSCR NKVSCADILT LATRDVVVAA GGPSYEVELG
160 170 180 190 200
RFDGLVSTAS SVEGNLPGPS DNVDKLNALF TKNKLTQEDM IALSAAHTLG
210 220 230 240 250
FAHCGKVFKR IHKFNGINSV DPTLNKAYAI ELQKACPKNV DPRIAINMDP
260 270 280 290 300
VTPKTFDNTY FKNLQQGKGL FTSDQVLFTD GRSRPTVNAW ASNSTAFNRA
310 320
FVIAMTKLGR VGVKNSSNGN IRRDCGAFN
Length:329
Mass (Da):35,756
Last modified:February 1, 1997 - v1
Checksum:iEDD91B0099A6A2C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98807 mRNA. Translation: CAA67339.1.
AL132978 Genomic DNA. Translation: CAB62104.1.
CP002686 Genomic DNA. Translation: AEE78611.1.
PIRiT45849.
RefSeqiNP_190565.1. NM_114856.2.
UniGeneiAt.19626.

Genome annotation databases

EnsemblPlantsiAT3G49960.1; AT3G49960.1; AT3G49960.
GeneIDi824158.
GrameneiAT3G49960.1; AT3G49960.1; AT3G49960.
KEGGiath:AT3G49960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98807 mRNA. Translation: CAA67339.1.
AL132978 Genomic DNA. Translation: CAB62104.1.
CP002686 Genomic DNA. Translation: AEE78611.1.
PIRiT45849.
RefSeqiNP_190565.1. NM_114856.2.
UniGeneiAt.19626.

3D structure databases

ProteinModelPortaliQ96510.
SMRiQ96510. Positions 27-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G49960.1.

Protein family/group databases

PeroxiBasei201. AtPrx35.

Proteomic databases

PaxDbiQ96510.
PRIDEiQ96510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G49960.1; AT3G49960.1; AT3G49960.
GeneIDi824158.
GrameneiAT3G49960.1; AT3G49960.1; AT3G49960.
KEGGiath:AT3G49960.

Organism-specific databases

TAIRiAT3G49960.

Phylogenomic databases

eggNOGiENOG410IJ72. Eukaryota.
ENOG4111FDS. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ96510.
KOiK00430.
OMAiKIFHANF.
OrthoDBiEOG09360FB9.
PhylomeDBiQ96510.

Enzyme and pathway databases

BioCyciARA:AT3G49960-MONOMER.

Miscellaneous databases

PROiQ96510.

Gene expression databases

ExpressionAtlasiQ96510. baseline and differential.
GenevisibleiQ96510. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER35_ARATH
AccessioniPrimary (citable) accession number: Q96510
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.