Q964D8 (BUP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-ureidopropionase
EC=3.5.1.6

Alternative name(s):
Beta-alanine synthase
N-carbamoyl-beta-alanine amidohydrolase

Gene names

Name:	pyd3
Synonyms:	bup1, upb1
ORF Names:	DDB_G0274123

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to, respectively, beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide.
Catalytic activity	N-carbamoyl-beta-alanine + H2O = beta-alanine + CO2 + NH3. Ref.1
Pathway	Amino-acid biosynthesis; beta-alanine biosynthesis.
Sequence similarities	Belongs to the CN hydrolase family. BUP subfamily. Contains 1 CN hydrolase domain.
Caution	Lacks the zinc-binding sites found in mammalian orthologs.

Ontologies

Keywords
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	beta-ureidopropionase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 391	390	Beta-ureidopropionase		PRO_0000327637
Regions
	Domain	75 – 369	295	CN hydrolase
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q964D8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C851253D45FC0948	FASTA	391	44,089
	10 20 30 40 50 60 MSKQFESVQA TLEKYIPAEE LSEVKRILYG YNRGHHVKSL PICQEALDLA NKNNFEIVAS 70 80 90 100 110 120 KVEADPEQLR KPRIVRLGII QNSIGAETTA PIQDQYLAIE AKIEKMIDAA GAMGVNVLCL 130 140 150 160 170 180 QETWHMPFAF CTREKYPWVE FAESASTGQS IKFIQRMARK YNMVIISPML ERDDVHASTI 190 200 210 220 230 240 HNTAVVVGNN GNIIGKSRKN HIPRTGDFNE STYYMESTLG HPVFETIYGK IAINICYGRH 250 260 270 280 290 300 HNLNWLAYGL NGAEIVFNPS ATVGELSEPM WGVEARNAAM TNNYFVGSIN RVGTEHFPNE 310 320 330 340 350 360 FTSGNGKPAH KDFGHFYGSS YFSSPDNCCT PSLSRVSDGL NISEVDLNLC QQVKDKWNFQ 370 380 390 MTARYELYAK FLTDYINPNY QPNIIKDPSM K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eukaryotic beta-alanine synthases are functionally related but have a high degree of structural diversity." Gojkovic Z., Sandrini M.P., Piskur J. Genetics 158:999-1011(2001) [PubMed: 11454750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-14; 62-70; 277-291 AND 371-391, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF333186 mRNA. Translation: AAK60519.1. AAFI02000012 Genomic DNA. Translation: EAL69954.1.
RefSeq	XP_644181.1. XM_639089.1.
3D structure databases
ProteinModelPortal	Q964D8.
SMR	Q964D8. Positions 5-387.
ModBase	Search...
Protein-protein interaction databases
STRING	Q964D8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185221; DDB0185221; DDB_G0274123.
GeneID	8619610.
GenomeReviews	Gene locus pyd3 in contig CM000151_GR.
KEGG	ddi:DDB_G0274123.
Organism-specific databases
dictyBase	DDB_G0274123. pyd3.
Phylogenomic databases
eggNOG	KOG0808.
GeneTree	EPrGT00050000005425.
HOGENOM	HBG742418.
OMA	VGTEVFP.
PhylomeDB	Q964D8.
ProtClustDB	CLSZ2729211.
Family and domain databases
InterPro	IPR003010. Ntlse/CNhydtse. [Graphical view]
Gene3D	G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
KO	K01431.
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUP1_DICDI

Accession

Primary (citable) accession number: Q964D8
Secondary accession number(s): Q554Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	December 1, 2001
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)