Reviewed, UniProtKB/Swiss-Prot Q96480 (P5CS_SOLLC)

Names and origin

Protein names

Recommended name:
    Delta-1-pyrroline-5-carboxylate synthetase
      Short name=P5CS
Including the following 2 domains:
    1- Recommended name:
            Glutamate 5-kinase
                Short name=GK
              EC=2.7.2.11
        Alternative name(s):
            Gamma-glutamyl kinase
    2- Recommended name:
            Gamma-glutamyl phosphate reductase
                Short name=GPR
              EC=1.2.1.41
        Alternative name(s):
            Glutamate-5-semialdehyde dehydrogenase
            Glutamyl-gamma-semialdehyde dehydrogenase

Gene names

Name:

PRO2

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.
Enzyme regulation	Feedback regulated by proline.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Tissue specificity	Expressed at high levels in leaves and is inducible in roots subjected to salt stress.
Sequence similarities	In the N-terminal section; belongs to the glutamate 5-kinase family. In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: EC glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 717	717	Delta-1-pyrroline-5-carboxylate synthetase		PRO_0000109775
Regions
	Region	1 – 296	296	Glutamate 5-kinase
	Region	297 – 717	421	Gamma-glutamyl phosphate reductase

Sequences

	Sequence		Length	Mass (Da)
	Q96480-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1E206428B69EEBCA	FASTA	717	77,590
	10 20 30 40 50 60 METVDSTRAF VKNVKRLIVK VGTAVVTRAD GRLALGRLGA LCEQLQELNS QGYEVILVTS 70 80 90 100 110 120 GAVGVGRQRL RYRKLLNSSF LDLQKPQTEL DGKACAAVGQ NGLMALYDSL FSQLDVTSAQ 130 140 150 160 170 180 LLVTDNDFRD PDFRRQLNDT VNSLLSLKVI PIFNENDAIS TRRAPYEDSS GIFWDNDSLA 190 200 210 220 230 240 ALLALELKAD LLVLLSDVDG LYSGPPRDPD SKLIYTYIKE IHERVITFGD KSRVGRGGMT 250 260 270 280 290 300 AKVKAAMYAA YAGIPVVITS GFATDNIIKV LHGERIGTLF HCDANKWASI GETDAREMAV 310 320 330 340 350 360 AARACSRRLQ ALSSQERSKI LQDIADALEA NEKAILAENE ADVVAAQQAG YEKSLISRLA 370 380 390 400 410 420 LNPGKISSLA NSVRVLSNMD EPLGHTLKRT EIADGFILEK SSSPLGVVLI IFESRPDALV 430 440 450 460 470 480 QIASLAVRSG NGLMLKGGKE AKRSNAILHK VITSAIPVSV GERLIGLVTS REEIPELLKL 490 500 510 520 530 540 DDVIDLVIPR GSNKLVSQIK ASTKIPVLGH ADGICHVYVD KSADMDMAKR ITVDAKIDYP 550 560 570 580 590 600 AACNAMETLL VHKDLAQNGG LNDLIVELQT KGVSLYGGPK ASSLLMIPEA RTFRHEYSSL 610 620 630 640 650 660 ACTVEVVEDV YAAIDHIHQH GSAHTDSIIT EDQEVAEVFL RQVDSAAVFH NASTRFSDGF 670 680 690 700 710 RFGLGAEVGI STGRIHARGP VGVEGLLTTK WLARGSGQIV DGDKSIVYSH KDLTQQG

References

[1]

"Cloning of tomPRO1 and tomPRO2 from Lycopersicon esculentum L.: coexistence of policistronic and monocistronic genes which encode the enzymes catalyzing the first two steps of proline biosynthesis." Maggio A., Garcia-Rios M., Fujita T., Bressan R.A., Joly R.J., Hasegawa M.P., Csonka L.N. Plant Gene Register PGR96-077 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ailsa Craig.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60267 mRNA. Translation: AAB67875.1.
PIR	T07422.
UniGene	Les.579
3D structure databases
SMR	Q96480. Positions 13-286, 296-714.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.41. 281054. 2.7.2.11. 281054.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR001048. Asp/Glu/Uridylate_kinase. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR001057. Glu_5kinase. IPR019797. Glutamate_5-kinase_CS. IPR005766. P5_carboxy_syn. IPR005715. ProB. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036429. P5C_syn. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01092. P5CS. 1 hit. TIGR00407. proA. 1 hit. TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CS_SOLLC

Accession

Primary (citable) accession number: Q96480

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)