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Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

PRO2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathway: L-proline biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Delta-1-pyrroline-5-carboxylate synthase (PRO2)
  2. Delta-1-pyrroline-5-carboxylate synthase (PRO2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei176 – 1761Substrate; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 1972ATPBy similarity
Nucleotide bindingi236 – 2427ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:PRO2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Delta-1-pyrroline-5-carboxylate synthasePRO_0000109775Add
BLAST

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Interactioni

Protein-protein interaction databases

STRINGi4081.Solyc08g043170.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ96480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Glutamate 5-kinaseAdd
BLAST
Regioni297 – 717421Gamma-glutamyl phosphate reductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Phylogenomic databases

InParanoidiQ96480.
KOiK12657.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR000965. GPR_dom.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METVDSTRAF VKNVKRLIVK VGTAVVTRAD GRLALGRLGA LCEQLQELNS
60 70 80 90 100
QGYEVILVTS GAVGVGRQRL RYRKLLNSSF LDLQKPQTEL DGKACAAVGQ
110 120 130 140 150
NGLMALYDSL FSQLDVTSAQ LLVTDNDFRD PDFRRQLNDT VNSLLSLKVI
160 170 180 190 200
PIFNENDAIS TRRAPYEDSS GIFWDNDSLA ALLALELKAD LLVLLSDVDG
210 220 230 240 250
LYSGPPRDPD SKLIYTYIKE IHERVITFGD KSRVGRGGMT AKVKAAMYAA
260 270 280 290 300
YAGIPVVITS GFATDNIIKV LHGERIGTLF HCDANKWASI GETDAREMAV
310 320 330 340 350
AARACSRRLQ ALSSQERSKI LQDIADALEA NEKAILAENE ADVVAAQQAG
360 370 380 390 400
YEKSLISRLA LNPGKISSLA NSVRVLSNMD EPLGHTLKRT EIADGFILEK
410 420 430 440 450
SSSPLGVVLI IFESRPDALV QIASLAVRSG NGLMLKGGKE AKRSNAILHK
460 470 480 490 500
VITSAIPVSV GERLIGLVTS REEIPELLKL DDVIDLVIPR GSNKLVSQIK
510 520 530 540 550
ASTKIPVLGH ADGICHVYVD KSADMDMAKR ITVDAKIDYP AACNAMETLL
560 570 580 590 600
VHKDLAQNGG LNDLIVELQT KGVSLYGGPK ASSLLMIPEA RTFRHEYSSL
610 620 630 640 650
ACTVEVVEDV YAAIDHIHQH GSAHTDSIIT EDQEVAEVFL RQVDSAAVFH
660 670 680 690 700
NASTRFSDGF RFGLGAEVGI STGRIHARGP VGVEGLLTTK WLARGSGQIV
710
DGDKSIVYSH KDLTQQG
Length:717
Mass (Da):77,590
Last modified:February 1, 1997 - v1
Checksum:i1E206428B69EEBCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60267 mRNA. Translation: AAB67875.1.
PIRiT07422.
RefSeqiNP_001233907.1. NM_001246978.2.
UniGeneiLes.579.

Genome annotation databases

GeneIDi544281.
KEGGisly:544281.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60267 mRNA. Translation: AAB67875.1.
PIRiT07422.
RefSeqiNP_001233907.1. NM_001246978.2.
UniGeneiLes.579.

3D structure databases

ProteinModelPortaliQ96480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc08g043170.2.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi544281.
KEGGisly:544281.

Phylogenomic databases

InParanoidiQ96480.
KOiK12657.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR000965. GPR_dom.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of tomPRO1 and tomPRO2 from Lycopersicon esculentum L.: coexistence of policistronic and monocistronic genes which encode the enzymes catalyzing the first two steps of proline biosynthesis."
    Maggio A., Garcia-Rios M., Fujita T., Bressan R.A., Joly R.J., Hasegawa M.P., Csonka L.N.
    Plant Gene Register PGR96-077
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ailsa Craig.

Entry informationi

Entry nameiP5CS_SOLLC
AccessioniPrimary (citable) accession number: Q96480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.