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Q96480

- P5CS_SOLLC

UniProt

Q96480 - P5CS_SOLLC

Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

PRO2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
    L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

    Enzyme regulationi

    Feedback regulated by proline.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei60 – 601SubstrateBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei176 – 1761Substrate; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi196 – 1972ATPBy similarity
    Nucleotide bindingi236 – 2427ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-EC
    3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00098; UER00359.
    UPA00098; UER00360.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-1-pyrroline-5-carboxylate synthase
    Short name:
    P5CS
    Including the following 2 domains:
    Glutamate 5-kinase (EC:2.7.2.11)
    Short name:
    GK
    Alternative name(s):
    Gamma-glutamyl kinase
    Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
    Short name:
    GPR
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
    Gene namesi
    Name:PRO2
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 717717Delta-1-pyrroline-5-carboxylate synthasePRO_0000109775Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96480.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 296296Glutamate 5-kinaseAdd
    BLAST
    Regioni297 – 717421Gamma-glutamyl phosphate reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
    In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

    Phylogenomic databases

    KOiK12657.

    Family and domain databases

    Gene3Di3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPiMF_00412. ProA.
    MF_00456. ProB.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036429. P5C_syn. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q96480-1 [UniParc]FASTAAdd to Basket

    « Hide

    METVDSTRAF VKNVKRLIVK VGTAVVTRAD GRLALGRLGA LCEQLQELNS    50
    QGYEVILVTS GAVGVGRQRL RYRKLLNSSF LDLQKPQTEL DGKACAAVGQ 100
    NGLMALYDSL FSQLDVTSAQ LLVTDNDFRD PDFRRQLNDT VNSLLSLKVI 150
    PIFNENDAIS TRRAPYEDSS GIFWDNDSLA ALLALELKAD LLVLLSDVDG 200
    LYSGPPRDPD SKLIYTYIKE IHERVITFGD KSRVGRGGMT AKVKAAMYAA 250
    YAGIPVVITS GFATDNIIKV LHGERIGTLF HCDANKWASI GETDAREMAV 300
    AARACSRRLQ ALSSQERSKI LQDIADALEA NEKAILAENE ADVVAAQQAG 350
    YEKSLISRLA LNPGKISSLA NSVRVLSNMD EPLGHTLKRT EIADGFILEK 400
    SSSPLGVVLI IFESRPDALV QIASLAVRSG NGLMLKGGKE AKRSNAILHK 450
    VITSAIPVSV GERLIGLVTS REEIPELLKL DDVIDLVIPR GSNKLVSQIK 500
    ASTKIPVLGH ADGICHVYVD KSADMDMAKR ITVDAKIDYP AACNAMETLL 550
    VHKDLAQNGG LNDLIVELQT KGVSLYGGPK ASSLLMIPEA RTFRHEYSSL 600
    ACTVEVVEDV YAAIDHIHQH GSAHTDSIIT EDQEVAEVFL RQVDSAAVFH 650
    NASTRFSDGF RFGLGAEVGI STGRIHARGP VGVEGLLTTK WLARGSGQIV 700
    DGDKSIVYSH KDLTQQG 717
    Length:717
    Mass (Da):77,590
    Last modified:February 1, 1997 - v1
    Checksum:i1E206428B69EEBCA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60267 mRNA. Translation: AAB67875.1.
    PIRiT07422.
    RefSeqiNP_001233907.1. NM_001246978.2.
    UniGeneiLes.579.

    Genome annotation databases

    GeneIDi544281.
    KEGGisly:544281.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60267 mRNA. Translation: AAB67875.1 .
    PIRi T07422.
    RefSeqi NP_001233907.1. NM_001246978.2.
    UniGenei Les.579.

    3D structure databases

    ProteinModelPortali Q96480.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 544281.
    KEGGi sly:544281.

    Phylogenomic databases

    KOi K12657.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    UPA00098 ; UER00360 .

    Family and domain databases

    Gene3Di 3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPi MF_00412. ProA.
    MF_00456. ProB.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036429. P5C_syn. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of tomPRO1 and tomPRO2 from Lycopersicon esculentum L.: coexistence of policistronic and monocistronic genes which encode the enzymes catalyzing the first two steps of proline biosynthesis."
      Maggio A., Garcia-Rios M., Fujita T., Bressan R.A., Joly R.J., Hasegawa M.P., Csonka L.N.
      Plant Gene Register PGR96-077
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Ailsa Craig.

    Entry informationi

    Entry nameiP5CS_SOLLC
    AccessioniPrimary (citable) accession number: Q96480
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3