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Q96480 (P5CS_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-1-pyrroline-5-carboxylate synthase

Short name=P5CS

Including the following 2 domains:

  1. Glutamate 5-kinase
    Short name=GK
    EC=2.7.2.11
    Alternative name(s):
    Gamma-glutamyl kinase
  2. Gamma-glutamyl phosphate reductase
    Short name=GPR
    EC=1.2.1.41
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
Gene names
Name:PRO2
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length717 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00456

Enzyme regulation

Feedback regulated by proline. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.

Tissue specificity

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Sequence similarities

In the N-terminal section; belongs to the glutamate 5-kinase family.

In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 717717Delta-1-pyrroline-5-carboxylate synthase HAMAP-Rule MF_00456
PRO_0000109775

Regions

Nucleotide binding196 – 1972ATP By similarity
Nucleotide binding236 – 2427ATP By similarity
Region1 – 296296Glutamate 5-kinase HAMAP-Rule MF_00456
Region297 – 717421Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00456

Sites

Binding site601Substrate By similarity
Binding site1571Substrate By similarity
Binding site1761Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q96480 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 1E206428B69EEBCA

FASTA71777,590
        10         20         30         40         50         60 
METVDSTRAF VKNVKRLIVK VGTAVVTRAD GRLALGRLGA LCEQLQELNS QGYEVILVTS 

        70         80         90        100        110        120 
GAVGVGRQRL RYRKLLNSSF LDLQKPQTEL DGKACAAVGQ NGLMALYDSL FSQLDVTSAQ 

       130        140        150        160        170        180 
LLVTDNDFRD PDFRRQLNDT VNSLLSLKVI PIFNENDAIS TRRAPYEDSS GIFWDNDSLA 

       190        200        210        220        230        240 
ALLALELKAD LLVLLSDVDG LYSGPPRDPD SKLIYTYIKE IHERVITFGD KSRVGRGGMT 

       250        260        270        280        290        300 
AKVKAAMYAA YAGIPVVITS GFATDNIIKV LHGERIGTLF HCDANKWASI GETDAREMAV 

       310        320        330        340        350        360 
AARACSRRLQ ALSSQERSKI LQDIADALEA NEKAILAENE ADVVAAQQAG YEKSLISRLA 

       370        380        390        400        410        420 
LNPGKISSLA NSVRVLSNMD EPLGHTLKRT EIADGFILEK SSSPLGVVLI IFESRPDALV 

       430        440        450        460        470        480 
QIASLAVRSG NGLMLKGGKE AKRSNAILHK VITSAIPVSV GERLIGLVTS REEIPELLKL 

       490        500        510        520        530        540 
DDVIDLVIPR GSNKLVSQIK ASTKIPVLGH ADGICHVYVD KSADMDMAKR ITVDAKIDYP 

       550        560        570        580        590        600 
AACNAMETLL VHKDLAQNGG LNDLIVELQT KGVSLYGGPK ASSLLMIPEA RTFRHEYSSL 

       610        620        630        640        650        660 
ACTVEVVEDV YAAIDHIHQH GSAHTDSIIT EDQEVAEVFL RQVDSAAVFH NASTRFSDGF 

       670        680        690        700        710 
RFGLGAEVGI STGRIHARGP VGVEGLLTTK WLARGSGQIV DGDKSIVYSH KDLTQQG 

« Hide

References

[1]"Cloning of tomPRO1 and tomPRO2 from Lycopersicon esculentum L.: coexistence of policistronic and monocistronic genes which encode the enzymes catalyzing the first two steps of proline biosynthesis."
Maggio A., Garcia-Rios M., Fujita T., Bressan R.A., Joly R.J., Hasegawa M.P., Csonka L.N.
Plant Gene Register PGR96-077
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Ailsa Craig.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60267 mRNA. Translation: AAB67875.1.
PIRT07422.
RefSeqNP_001233907.1. NM_001246978.2.
UniGeneLes.579.

3D structure databases

ProteinModelPortalQ96480.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID544281.
KEGGsly:544281.

Phylogenomic databases

KOK12657.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.
UPA00098; UER00360.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPMF_00456. ProB.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFPIRSF036429. P5C_syn. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5CS_SOLLC
AccessionPrimary (citable) accession number: Q96480
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways