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Q96480

- P5CS_SOLLC

UniProt

Q96480 - P5CS_SOLLC

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Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene
PRO2
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Enzyme regulationi

Feedback regulated by proline.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601Substrate By similarity
Binding sitei157 – 1571Substrate By similarity
Binding sitei176 – 1761Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 1972ATP By similarity
Nucleotide bindingi236 – 2427ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:PRO2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Delta-1-pyrroline-5-carboxylate synthaseUniRule annotationPRO_0000109775Add
BLAST

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Structurei

3D structure databases

ProteinModelPortaliQ96480.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Glutamate 5-kinaseUniRule annotationAdd
BLAST
Regioni297 – 717421Gamma-glutamyl phosphate reductaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Phylogenomic databases

KOiK12657.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96480-1 [UniParc]FASTAAdd to Basket

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METVDSTRAF VKNVKRLIVK VGTAVVTRAD GRLALGRLGA LCEQLQELNS    50
QGYEVILVTS GAVGVGRQRL RYRKLLNSSF LDLQKPQTEL DGKACAAVGQ 100
NGLMALYDSL FSQLDVTSAQ LLVTDNDFRD PDFRRQLNDT VNSLLSLKVI 150
PIFNENDAIS TRRAPYEDSS GIFWDNDSLA ALLALELKAD LLVLLSDVDG 200
LYSGPPRDPD SKLIYTYIKE IHERVITFGD KSRVGRGGMT AKVKAAMYAA 250
YAGIPVVITS GFATDNIIKV LHGERIGTLF HCDANKWASI GETDAREMAV 300
AARACSRRLQ ALSSQERSKI LQDIADALEA NEKAILAENE ADVVAAQQAG 350
YEKSLISRLA LNPGKISSLA NSVRVLSNMD EPLGHTLKRT EIADGFILEK 400
SSSPLGVVLI IFESRPDALV QIASLAVRSG NGLMLKGGKE AKRSNAILHK 450
VITSAIPVSV GERLIGLVTS REEIPELLKL DDVIDLVIPR GSNKLVSQIK 500
ASTKIPVLGH ADGICHVYVD KSADMDMAKR ITVDAKIDYP AACNAMETLL 550
VHKDLAQNGG LNDLIVELQT KGVSLYGGPK ASSLLMIPEA RTFRHEYSSL 600
ACTVEVVEDV YAAIDHIHQH GSAHTDSIIT EDQEVAEVFL RQVDSAAVFH 650
NASTRFSDGF RFGLGAEVGI STGRIHARGP VGVEGLLTTK WLARGSGQIV 700
DGDKSIVYSH KDLTQQG 717
Length:717
Mass (Da):77,590
Last modified:February 1, 1997 - v1
Checksum:i1E206428B69EEBCA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60267 mRNA. Translation: AAB67875.1.
PIRiT07422.
RefSeqiNP_001233907.1. NM_001246978.2.
UniGeneiLes.579.

Genome annotation databases

GeneIDi544281.
KEGGisly:544281.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60267 mRNA. Translation: AAB67875.1 .
PIRi T07422.
RefSeqi NP_001233907.1. NM_001246978.2.
UniGenei Les.579.

3D structure databases

ProteinModelPortali Q96480.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 544281.
KEGGi sly:544281.

Phylogenomic databases

KOi K12657.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
UPA00098 ; UER00360 .

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPi MF_00412. ProA.
MF_00456. ProB.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view ]
PIRSFi PIRSF036429. P5C_syn. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of tomPRO1 and tomPRO2 from Lycopersicon esculentum L.: coexistence of policistronic and monocistronic genes which encode the enzymes catalyzing the first two steps of proline biosynthesis."
    Maggio A., Garcia-Rios M., Fujita T., Bressan R.A., Joly R.J., Hasegawa M.P., Csonka L.N.
    Plant Gene Register PGR96-077
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ailsa Craig.

Entry informationi

Entry nameiP5CS_SOLLC
AccessioniPrimary (citable) accession number: Q96480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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