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Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

PRO2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Delta-1-pyrroline-5-carboxylate synthase (101244293), Delta-1-pyrroline-5-carboxylate synthase, Delta-1-pyrroline-5-carboxylate synthase (PRO2)
  2. Delta-1-pyrroline-5-carboxylate synthase (101244293), Delta-1-pyrroline-5-carboxylate synthase, Delta-1-pyrroline-5-carboxylate synthase (PRO2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60SubstrateBy similarity1
Binding sitei157SubstrateBy similarity1
Binding sitei176Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi196 – 197ATPBy similarity2
Nucleotide bindingi236 – 242ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359
UPA00098; UER00360

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:PRO2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Unplaced

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097751 – 717Delta-1-pyrroline-5-carboxylate synthaseAdd BLAST717

Proteomic databases

PaxDbiQ96480
PRIDEiQ96480

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Gene expression databases

ExpressionAtlasiQ96480 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi4081.Solyc08g043170.2.1

Structurei

3D structure databases

ProteinModelPortaliQ96480
SMRiQ96480
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 296Glutamate 5-kinaseAdd BLAST296
Regioni297 – 717Gamma-glutamyl phosphate reductaseAdd BLAST421

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Phylogenomic databases

eggNOGiKOG1154 Eukaryota
KOG4165 Eukaryota
COG0014 LUCA
COG0263 LUCA
InParanoidiQ96480
KOiK12657

Family and domain databases

CDDicd07079 ALDH_F18-19_ProA-GPR, 1 hit
Gene3Di3.40.1160.10, 1 hit
3.40.309.10, 1 hit
3.40.605.10, 2 hits
HAMAPiMF_00412 ProA, 1 hit
MF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR020593 G-glutamylP_reductase_CS
IPR001057 Glu/AcGlu_kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR000965 GPR_dom
IPR005766 P5_carboxy_syn
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF00171 Aldedh, 1 hit
PIRSFiPIRSF036429 P5C_syn, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
SSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR01092 P5CS, 1 hit
TIGR00407 proA, 1 hit
TIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS01223 PROA, 1 hit

Sequencei

Sequence statusi: Complete.

Q96480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METVDSTRAF VKNVKRLIVK VGTAVVTRAD GRLALGRLGA LCEQLQELNS
60 70 80 90 100
QGYEVILVTS GAVGVGRQRL RYRKLLNSSF LDLQKPQTEL DGKACAAVGQ
110 120 130 140 150
NGLMALYDSL FSQLDVTSAQ LLVTDNDFRD PDFRRQLNDT VNSLLSLKVI
160 170 180 190 200
PIFNENDAIS TRRAPYEDSS GIFWDNDSLA ALLALELKAD LLVLLSDVDG
210 220 230 240 250
LYSGPPRDPD SKLIYTYIKE IHERVITFGD KSRVGRGGMT AKVKAAMYAA
260 270 280 290 300
YAGIPVVITS GFATDNIIKV LHGERIGTLF HCDANKWASI GETDAREMAV
310 320 330 340 350
AARACSRRLQ ALSSQERSKI LQDIADALEA NEKAILAENE ADVVAAQQAG
360 370 380 390 400
YEKSLISRLA LNPGKISSLA NSVRVLSNMD EPLGHTLKRT EIADGFILEK
410 420 430 440 450
SSSPLGVVLI IFESRPDALV QIASLAVRSG NGLMLKGGKE AKRSNAILHK
460 470 480 490 500
VITSAIPVSV GERLIGLVTS REEIPELLKL DDVIDLVIPR GSNKLVSQIK
510 520 530 540 550
ASTKIPVLGH ADGICHVYVD KSADMDMAKR ITVDAKIDYP AACNAMETLL
560 570 580 590 600
VHKDLAQNGG LNDLIVELQT KGVSLYGGPK ASSLLMIPEA RTFRHEYSSL
610 620 630 640 650
ACTVEVVEDV YAAIDHIHQH GSAHTDSIIT EDQEVAEVFL RQVDSAAVFH
660 670 680 690 700
NASTRFSDGF RFGLGAEVGI STGRIHARGP VGVEGLLTTK WLARGSGQIV
710
DGDKSIVYSH KDLTQQG
Length:717
Mass (Da):77,590
Last modified:February 1, 1997 - v1
Checksum:i1E206428B69EEBCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60267 mRNA Translation: AAB67875.1
PIRiT07422
RefSeqiNP_001233907.1, NM_001246978.2
UniGeneiLes.23791
Les.579

Genome annotation databases

GeneIDi544281
KEGGisly:544281

Similar proteinsi

Entry informationi

Entry nameiP5CS_SOLLC
AccessioniPrimary (citable) accession number: Q96480
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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