Q96423 (TCMO_GLYEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-cinnamate 4-monooxygenase
EC=1.14.13.11

Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H

Gene names

Name:	CYP73A14
Synonyms:	CYP73

Organism

Glycyrrhiza echinata (Licorice)

Taxonomic identifier

46348 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Galegeae › Glycyrrhiza

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Induction	By fungal elicitor.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052245
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96423 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 83C6963D5E0741B2	FASTA	505	57,988
	10 20 30 40 50 60 MDLLLLEKTL LGLFIAAITA IAISKLRGRR FKLPPGPIPV PIFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKRFGDIFL LRMGQRNLVV VSSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRFGW ESEAASVVDD VRRNPDAAAG GIVLRRRLQL 190 200 210 220 230 240 MMYNNMYRIM FDRRFESEED PLFVKLKALN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK 250 260 270 280 290 300 ICKEVKERRL KLFKDYFVDE RMKLESTKST SNEGLKCAID HILDAQKKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHPEIQK KVRDEIDRVL GPGHQVTEPD MQKLPYLQAV 370 380 390 400 410 420 IKETLRLRMA IPLLVPHMNL HDAKLGGYDI PAESKILVNA WWLANNPANW KRPEEFRPER 430 440 450 460 470 480 FLEEESHVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT LGRLVQNFEL LPPPGQSKLD 490 500 TAEKGGQFSL HILKHSTIVA KPRSF

References

[1]	"Cloning of cytochrome P450 cDNAs from cultured Glycyrrhiza echinata L. cells and their transcriptional activation by elicitor-treatment." Akashi T., Aoki T., Takahashi T., Kameya N., Nakamura I., Ayabe S. Plant Sci. 126:39-47(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87520 mRNA. Translation: BAA13414.1.
3D structure databases
ProteinModelPortal	Q96423.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_GLYEC

Accession

Primary (citable) accession number: Q96423

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 28, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program