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Protein

Pectate lyase 1

Gene
N/A
Organism
Chamaecyparis obtusa (Hinoki false-cypress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has pectate lyase activity.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Pathwayi: pectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Pectate lyase 1
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi170 – 1701CalciumBy similarity
Metal bindingi194 – 1941CalciumBy similarity
Metal bindingi198 – 1981CalciumBy similarity
Active sitei250 – 2501Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 1 (EC:4.2.2.2)
Alternative name(s):
Major pollen allergen Cha o 1
Allergen: Cha o 1
OrganismiChamaecyparis obtusa (Hinoki false-cypress)
Taxonomic identifieri13415 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeChamaecyparis

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei197. Cha o 1.
3186. Cha o 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 375354Pectate lyase 1PRO_0000024907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 45By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence analysis
Disulfide bondi128 ↔ 147By similarity
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence analysis
Disulfide bondi306 ↔ 312By similarity
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ96385.
SMRiQ96385. Positions 22-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASCTLLAVL VFLCAIVSCF SDNPIDSCWR GDANWDQNRM KLADCAVGFG
60 70 80 90 100
SSAMGGKGGA FYTVTSSDDD PVNPAPGTLR YGATRERSLW IIFSKNLNIK
110 120 130 140 150
LNMPLYIAGN KTIDGRGAEV HIGNGGPCLF MRTVSHVILH GLNIHGCNTS
160 170 180 190 200
VSGNVLISEA SGVVPVHAQD GDAITMRNVT DVWIDHNSLS DSSDGLVDVT
210 220 230 240 250
LASTGVTISN NHFFNHHKVM LLGHSDIYSD DKSMKVTVAF NQFGPNAGQR
260 270 280 290 300
MPRARYGLIH VANNNYDPWS IYAIGGSSNP TILSEGNSFT APNDSDKKEV
310 320 330 340 350
TRRVGCESPS TCANWVWRST QDSFNNGAYF VSSGKNEGTN IYNNNEAFKV
360 370
ENGSAAPQLT KNAGVLTCIL SKPCS
Length:375
Mass (Da):40,258
Last modified:February 1, 1997 - v1
Checksum:i81CD91DF7066DBBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45404 mRNA. Translation: BAA08246.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45404 mRNA. Translation: BAA08246.1.

3D structure databases

ProteinModelPortaliQ96385.
SMRiQ96385. Positions 22-367.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei197. Cha o 1.
3186. Cha o 1.0101.
CAZyiPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification, characterization and molecular cloning of Cha o 1, a major allergen of Chamaecyparis obtusa (Japanese cypress) pollen."
    Suzuki M., Komiyama N., Itoh M., Itoh H., Sone T., Kuno K., Takagi I., Ohta N.
    Mol. Immunol. 33:451-460(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ALLERGEN.
    Tissue: Pollen.

Entry informationi

Entry nameiPLY1_CHAOB
AccessioniPrimary (citable) accession number: Q96385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: October 14, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.