Q96385 (MPAC1_CHAOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Major pollen allergen Cha o 1 Alternative name(s): Allergen=Cha o 1
Organism	Chamaecyparis obtusa (Hinoki false-cypress)
Taxonomic identifier	13415 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Coniferophyta › Coniferopsida › Coniferales › Cupressaceae › Chamaecyparis

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Allergenic properties	Causes an allergic reaction in human. Ref.1
Sequence similarities	Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
Disease	Allergen
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 375	354	Major pollen allergen Cha o 1		PRO_0000024907
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential
	Disulfide bond	28 ↔ 45		By similarity
	Disulfide bond	128 ↔ 147		By similarity
	Disulfide bond	306 ↔ 312		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96385 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 81CD91DF7066DBBF	FASTA	375	40,258
	10 20 30 40 50 60 MASCTLLAVL VFLCAIVSCF SDNPIDSCWR GDANWDQNRM KLADCAVGFG SSAMGGKGGA 70 80 90 100 110 120 FYTVTSSDDD PVNPAPGTLR YGATRERSLW IIFSKNLNIK LNMPLYIAGN KTIDGRGAEV 130 140 150 160 170 180 HIGNGGPCLF MRTVSHVILH GLNIHGCNTS VSGNVLISEA SGVVPVHAQD GDAITMRNVT 190 200 210 220 230 240 DVWIDHNSLS DSSDGLVDVT LASTGVTISN NHFFNHHKVM LLGHSDIYSD DKSMKVTVAF 250 260 270 280 290 300 NQFGPNAGQR MPRARYGLIH VANNNYDPWS IYAIGGSSNP TILSEGNSFT APNDSDKKEV 310 320 330 340 350 360 TRRVGCESPS TCANWVWRST QDSFNNGAYF VSSGKNEGTN IYNNNEAFKV ENGSAAPQLT 370 KNAGVLTCIL SKPCS

References

[1]

"Purification, characterization and molecular cloning of Cha o 1, a major allergen of Chamaecyparis obtusa (Japanese cypress) pollen." Suzuki M., Komiyama N., Itoh M., Itoh H., Sone T., Kuno K., Takagi I., Ohta N. Mol. Immunol. 33:451-460(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ALLERGEN. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45404 mRNA. Translation: BAA08246.1.
3D structure databases
ProteinModelPortal	Q96385.
SMR	Q96385. Positions 22-367.
ModBase	Search...
Protein family/group databases
Allergome	197. Cha o 1. 3186. Cha o 1.0101.
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR002022. Amb_allergen_dom. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MPAC1_CHAOB

Accession

Primary (citable) accession number: Q96385

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program