Q96375 (ABA2_CAPAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Zeaxanthin epoxidase, chloroplastic EC=1.14.13.90 Alternative name(s): Beta-cyclohexenyl epoxidase Xanthophyll epoxidase
Organism	Capsicum annuum (Bell pepper)
Taxonomic identifier	4072 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Acts also on beta-cryptoxanthin. Involved in the epoxidation of zeaxanthin.
Catalytic activity	Zeaxanthin + NAD(P)H + O2 = antheraxanthin + NAD(P)+ + H2O. Antheraxanthin + NAD(P)H + O2 = violaxanthin + NAD(P)+ + H2O.
Cofactor	FAD Potential.
Pathway	Plant hormone biosynthesis; abscisate biosynthesis.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein.
Induction	Up-regulated by oxidative stress and when chloroplasts undergo differentiation into chromoplasts.
Sequence similarities	Contains 1 FHA domain.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	antheraxanthin epoxidase activity Inferred from electronic annotation. Source: EC zeaxanthin epoxidase [overall] activity Inferred from electronic annotation. Source: EC zeaxanthin epoxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 660	611	Zeaxanthin epoxidase, chloroplastic		PRO_0000020609
Regions
	Domain	570 – 631	62	FHA
	Nucleotide binding	79 – 107	29	FAD Potential
	Nucleotide binding	357 – 370	14	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q96375 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 80AF5D8F7D381121	FASTA	660	71,952
	10 20 30 40 50 60 MYASSARDGI PGKWCNARRK QLPLLISKDF PAELYHSLPC KSLENGHIKK VKGVKATLAE 70 80 90 100 110 120 APATPTEKSN SEVPQKKLKV LVAGGGIGGL VFALAGKKRG FDVLVFERDI SAIRGEGQYR 130 140 150 160 170 180 GPIQIQSNAL AALEAIDMDV AEEIMNAGCI TGQRINGLVD GISGNWYCKF DTFTPAVERG 190 200 210 220 230 240 LPVTRVISRM TLQQILARLQ GEDVIMNESH VVNFADDGET VTVNPELCQQ YTGDLLVGAD 250 260 270 280 290 300 GIRSKVRTNL FGPSELTYSG YTCYTGIADF VPADIDTAGY RVFLGHKQYF VSSDVGGGKM 310 320 330 340 350 360 QWYAFHNEPA GGVDAPNGKK ERLLKIFGGW CDNVIDLSVA TDEDAILRRD IYDRPPTFSW 370 380 390 400 410 420 GKGRVTLLGD SVHAMQPNLG QGGCMAIEDS YQLALELEKA WSRSAESGSP MDVISSLRSY 430 440 450 460 470 480 ESARKLRVGV IHGLARMAAI MASAYKAYLG VGLGPLSFIT KFRIPHPGRV GGRFFIDLGM 490 500 510 520 530 540 PLMLSWVLGG NGEKLEGRIQ HCRLSEKAND QLRNWFEDDD ALERATDAEW LLLPAGNSNA 550 560 570 580 590 600 ALETLVLSRD ENMPCTIGSV SHANIPGKSV VIPLSQVSDM HARISYNGGA FLGTAFRSDH 610 620 630 640 650 660 GTWFIDNEGR RYRVSPNFPM RFHSSDVIVF GSDKAAFRIK AMKFAPKTAA KEDRQAVGAA

References

[1]

"Xanthophyll biosynthesis. Cloning, expression, functional reconstitution, and regulation of beta-cyclohexenyl carotenoid epoxidase from pepper (Capsicum annuum)." Bouvier F., D'Harlingue A., Hugueney P., Marin E., Marion-Poll A., Camara B. J. Biol. Chem. 271:28861-28867(1996) [PubMed: 8910532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Yolo Wonder.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91491 mRNA. Translation: CAA62795.1.
PIR	T09537.
3D structure databases
ProteinModelPortal	Q96375.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-2601.
Family and domain databases
InterPro	IPR000253. FHA_dom. IPR002938. mOase_FAD-bd. IPR003042. Rng_hydrolase-like. IPR008984. SMAD_FHA_domain. IPR017079. Zeaxanthin_epoxidase. [Graphical view]
Gene3D	G3DSA:2.60.200.20. FHA. 1 hit.
Pfam	PF01494. FAD_binding_3. 2 hits. PF00498. FHA. 1 hit. [Graphical view]
PIRSF	PIRSF036989. Zeaxanthin_epoxidase. 1 hit.
PRINTS	PR00420. RNGMNOXGNASE.
SUPFAM	SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS50006. FHA_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABA2_CAPAN

Accession

Primary (citable) accession number: Q96375

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	June 28, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program