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Protein

Flavonol synthase/flavanone 3-hydroxylase

Gene

FLS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin. In vitro catalyzes the oxidation of both enantiomers of naringenin to give both cis- and trans-dihydrokaempferol.9 Publications

Catalytic activityi

A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O.By similarity
A (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • L-ascorbateBy similarityNote: Binds 1 ascorbate molecule per subunit.By similarity
  • Fe2+CuratedNote: Binds 1 Fe2+ ion per subunit.Curated

Kineticsi

  1. KM=59 µM for dihydroquercetin1 Publication

    Pathwayi: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi221Iron; catalyticCurated1
    Metal bindingi223Iron; catalyticCurated1
    Metal bindingi277Iron; catalyticCurated1

    GO - Molecular functioni

    GO - Biological processi

    • flavonoid biosynthetic process Source: TAIR
    • response to auxin Source: TAIR
    • response to karrikin Source: TAIR

    Keywordsi

    Molecular functionDioxygenase, Oxidoreductase
    Biological processFlavonoid biosynthesis
    LigandIron, Metal-binding, Vitamin C

    Enzyme and pathway databases

    BRENDAi1.14.11.23. 399.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flavonol synthase/flavanone 3-hydroxylase (EC:1.14.11.23By similarity, EC:1.14.11.9By similarity)
    Alternative name(s):
    FLS 1
    Gene namesi
    Name:FLS1
    Synonyms:FLS
    Ordered Locus Names:At5g08640
    ORF Names:MAH20.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G08640.
    TAIRilocus:2159542. AT5G08640.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Accumulation of anthocyanins and glycosylated forms of dihydroflavonols, and drastic reduction of kaempferol, quercitin and favonol glycosides.3 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi132H → F: Slightly increases activity. 2 Publications1
    Mutagenesisi132H → Y: Slightly decreases activity. 2 Publications1
    Mutagenesisi134F → A: Reduces activity 7-fold. 1 Publication1
    Mutagenesisi134F → L: Reduces activity 2-fold. 1 Publication1
    Mutagenesisi202K → M: Reduces activity 25-fold. 1 Publication1
    Mutagenesisi202K → R: Reduces activity 8-fold. 1 Publication1
    Mutagenesisi221H → W: Loss of activity. 1 Publication1
    Mutagenesisi223D → E: Loss of activity. 1 Publication1
    Mutagenesisi277H → F: Loss of activity. 1 Publication1
    Mutagenesisi287R → K: Loss of activity. 1 Publication1
    Mutagenesisi289S → T: Reduces activity 2-fold. 1 Publication1
    Mutagenesisi293F → A or L: Reduces activity 12-fold. 1 Publication1
    Mutagenesisi295E → L: Reduces activity 15-fold. 1 Publication1
    Mutagenesisi295E → Q: Reduces activity 2-fold. 1 Publication1
    Mutagenesisi329K → N: Reduces activity 4-fold. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000672911 – 336Flavonol synthase/flavanone 3-hydroxylaseAdd BLAST336

    Proteomic databases

    PaxDbiQ96330.
    PRIDEiQ96330.

    Expressioni

    Tissue specificityi

    Expressed in young seedlings (at protein level). Expressed in roots, emerging leaves, shoot-root transition zone, trichomes, flowers and siliques. In cotyledons, expressed mostly on the adaxial side and only in guard cells on the abaxial side.4 Publications

    Inductioni

    By light, auxin and 1-aminocyclopropane-1-carboxylic acid (ACC).4 Publications

    Gene expression databases

    ExpressionAtlasiQ96330. baseline and differential.
    GenevisibleiQ96330. AT.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CHSP131143EBI-1774454,EBI-1546775

    Protein-protein interaction databases

    BioGridi16043. 4 interactors.
    IntActiQ96330. 3 interactors.
    MINTiMINT-8184556.
    STRINGi3702.AT5G08640.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96330.
    SMRiQ96330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini196 – 296Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni204 – 2062-oxoglutarate bindingBy similarity3
    Regioni287 – 2892-oxoglutarate bindingCurated3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG0143. Eukaryota.
    COG3491. LUCA.
    InParanoidiQ96330.
    KOiK05278.
    OMAiPPREKIV.
    OrthoDBiEOG09360FVF.
    PhylomeDBiQ96330.

    Family and domain databases

    Gene3Di2.60.120.330. 1 hit.
    InterProiView protein in InterPro
    IPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    PfamiView protein in Pfam
    PF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    PROSITEiView protein in PROSITE
    PS51471. FE2OG_OXY. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q96330-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEVERVQDIS SSSLLTEAIP LEFIRSEKEQ PAITTFRGPT PAIPVVDLSD
    60 70 80 90 100
    PDEESVRRAV VKASEEWGLF QVVNHGIPTE LIRRLQDVGR KFFELPSSEK
    110 120 130 140 150
    ESVAKPEDSK DIEGYGTKLQ KDPEGKKAWV DHLFHRIWPP SCVNYRFWPK
    160 170 180 190 200
    NPPEYREVNE EYAVHVKKLS ETLLGILSDG LGLKRDALKE GLGGEMAEYM
    210 220 230 240 250
    MKINYYPPCP RPDLALGVPA HTDLSGITLL VPNEVPGLQV FKDDHWFDAE
    260 270 280 290 300
    YIPSAVIVHI GDQILRLSNG RYKNVLHRTT VDKEKTRMSW PVFLEPPREK
    310 320 330
    IVGPLPELTG DDNPPKFKPF AFKDYSYRKL NKLPLD
    Length:336
    Mass (Da):38,282
    Last modified:February 1, 1997 - v1
    Checksum:i3283E3AFE603D2A9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U72631 Genomic DNA. Translation: AAB17393.1.
    U84258 Genomic DNA. Translation: AAC69362.1.
    U84259 mRNA. Translation: AAC69363.1.
    U84260 mRNA. Translation: AAB41504.1.
    AB006697 Genomic DNA. Translation: BAB10013.1.
    CP002688 Genomic DNA. Translation: AED91332.1.
    CP002688 Genomic DNA. Translation: AED91333.1.
    AY058068 mRNA. Translation: AAL24176.1.
    BT000494 mRNA. Translation: AAN18063.1.
    AY086328 mRNA. Translation: AAM64397.1.
    RefSeqiNP_001190266.1. NM_001203337.1.
    NP_196481.1. NM_120951.3.
    UniGeneiAt.8771.

    Genome annotation databases

    EnsemblPlantsiAT5G08640.1; AT5G08640.1; AT5G08640.
    AT5G08640.2; AT5G08640.2; AT5G08640.
    GeneIDi830765.
    GrameneiAT5G08640.1; AT5G08640.1; AT5G08640.
    AT5G08640.2; AT5G08640.2; AT5G08640.
    KEGGiath:AT5G08640.

    Similar proteinsi

    Entry informationi

    Entry nameiFLS1_ARATH
    AccessioniPrimary (citable) accession number: Q96330
    Secondary accession number(s): O04730
    , O04731, O04732, O04830, O04831, O04832
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: February 1, 1997
    Last modified: June 7, 2017
    This is version 130 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families