Flavonol synthase/flavanone 3-hydroxylase - Arabidopsis thaliana (Mouse-ear cress)

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Q96330 (FLS1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Flavonol synthase/flavanone 3-hydroxylase
EC=1.14.11.23
EC=1.14.11.9

Alternative name(s):
FLS 1

Gene names

Name:	FLS1
Ordered Locus Names:	At5g08640
ORF Names:	MAH20.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.
Catalytic activity	A dihydroflavonol + 2-oxoglutarate + O₂ = a flavonol + succinate + CO₂ + H₂O. A flavanone + 2-oxoglutarate + O₂ = a dihydroflavonol + succinate + CO₂.
Cofactor	Binds 1 ascorbate molecule per subunit. Binds 1 iron ion per subunit.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to auxin stimulus Inferred from expression pattern. Source: TAIR response to karrikin Inferred from expression pattern. Source: TAIR
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activity Inferred from direct assay. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 336

336

Flavonol synthase/flavanone 3-hydroxylase

PRO_0000067291

Regions

Domain

196 – 296

101

Fe2OG dioxygenase

Sites

Metal binding

221

Iron By similarity

Metal binding

223

Iron By similarity

Metal binding

277

Iron By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q96330 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 3283E3AFE603D2A9
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FASTA

336

38,282

        10         20         30         40         50         60 
MEVERVQDIS SSSLLTEAIP LEFIRSEKEQ PAITTFRGPT PAIPVVDLSD PDEESVRRAV 

        70         80         90        100        110        120 
VKASEEWGLF QVVNHGIPTE LIRRLQDVGR KFFELPSSEK ESVAKPEDSK DIEGYGTKLQ 

       130        140        150        160        170        180 
KDPEGKKAWV DHLFHRIWPP SCVNYRFWPK NPPEYREVNE EYAVHVKKLS ETLLGILSDG 

       190        200        210        220        230        240 
LGLKRDALKE GLGGEMAEYM MKINYYPPCP RPDLALGVPA HTDLSGITLL VPNEVPGLQV 

       250        260        270        280        290        300 
FKDDHWFDAE YIPSAVIVHI GDQILRLSNG RYKNVLHRTT VDKEKTRMSW PVFLEPPREK 

       310        320        330 
IVGPLPELTG DDNPPKFKPF AFKDYSYRKL NKLPLD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of flavonol synthase and leucoanthocyanidin dioxygenase genes in Arabidopsis. Further evidence for differential regulation of 'early' and 'late' genes." Pelletier M.K., Murrell J.R., Shirley B.W. Plant Physiol. 113:1437-1445(1997) [PubMed: 9112784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[2]	"Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes." Wisman E., Hartmann U., Sagasser M., Baumann E., Palme K., Hahlbrock K., Saedler H., Weisshaar B. Proc. Natl. Acad. Sci. U.S.A. 95:12432-12437(1998) [PubMed: 9770503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia and cv. Landsberg erecta.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72631 Genomic DNA. Translation: AAB17393.1. U84258 Genomic DNA. Translation: AAC69362.1. U84259 mRNA. Translation: AAC69363.1. U84260 mRNA. Translation: AAB41504.1. AB006697 Genomic DNA. Translation: BAB10013.1. CP002688 Genomic DNA. Translation: AED91332.1. CP002688 Genomic DNA. Translation: AED91333.1. AY058068 mRNA. Translation: AAL24176.1. BT000494 mRNA. Translation: AAN18063.1. AY086328 mRNA. Translation: AAM64397.1.
IPI	IPI00532962.
RefSeq	NP_001190266.1. NM_001203337.1. NP_196481.1. NM_120951.2.
UniGene	At.8771.
3D structure databases
ProteinModelPortal	Q96330.
SMR	Q96330. Positions 2-334.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96330. 3 interactions.
STRING	Q96330.
Proteomic databases
PRIDE	Q96330.
ProMEX	Q96330.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G08640.1; AT5G08640.1; AT5G08640. AT5G08640.2; AT5G08640.2; AT5G08640.
GeneID	830765.
GenomeReviews	Gene locus AT5G08640 in contig BA000015_GR.
KEGG	ath:AT5G08640.
NMPDR	fig\|3702.1.peg.23017.
Organism-specific databases
TAIR	At5g08640.
Phylogenomic databases
GeneTree	EPGT00070000028021.
InParanoid	Q96330.
OMA	EWGLFQV.
PhylomeDB	Q96330.
ProtClustDB	PLN02704.
Gene expression databases
ArrayExpress	Q96330.
Genevestigator	Q96330.
GermOnline	AT5G08640. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
KO	K05278.
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLS1_ARATH

Accession

Primary (citable) accession number: Q96330
Secondary accession number(s): O04730

O04832

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	December 14, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents