Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q96330 (FLS1_ARATH)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Flavonol synthase/flavanone 3-hydroxylase
    EC=1.14.11.23
    EC=1.14.11.9
Alternative name(s):
    FLS 1
Gene names
Name: FLS1
Ordered Locus Names: At5g08640
ORF Names: MAH20.20
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.

Catalytic activity

A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O.

A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.

Cofactor

Binds 1 ascorbate molecule per subunit.

Binds 1 iron ion per subunit.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Flavonol synthase/flavanone 3-hydroxylase
PRO_0000067291

Sites

Metal binding2211Iron By similarity
Metal binding2231Iron By similarity
Metal binding2771Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q96330-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 3283E3AFE603D2A9

FASTA33638,282
        10         20         30         40         50         60 
MEVERVQDIS SSSLLTEAIP LEFIRSEKEQ PAITTFRGPT PAIPVVDLSD PDEESVRRAV 

        70         80         90        100        110        120 
VKASEEWGLF QVVNHGIPTE LIRRLQDVGR KFFELPSSEK ESVAKPEDSK DIEGYGTKLQ 

       130        140        150        160        170        180 
KDPEGKKAWV DHLFHRIWPP SCVNYRFWPK NPPEYREVNE EYAVHVKKLS ETLLGILSDG 

       190        200        210        220        230        240 
LGLKRDALKE GLGGEMAEYM MKINYYPPCP RPDLALGVPA HTDLSGITLL VPNEVPGLQV 

       250        260        270        280        290        300 
FKDDHWFDAE YIPSAVIVHI GDQILRLSNG RYKNVLHRTT VDKEKTRMSW PVFLEPPREK 

       310        320        330 
IVGPLPELTG DDNPPKFKPF AFKDYSYRKL NKLPLD 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of flavonol synthase and leucoanthocyanidin dioxygenase genes in Arabidopsis. Further evidence for differential regulation of 'early' and 'late' genes."
Pelletier M.K., Murrell J.R., Shirley B.W.
Plant Physiol. 113:1437-1445(1997) [PubMed: 9112784] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[2]"Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes."
Wisman E., Hartmann U., Sagasser M., Baumann E., Palme K., Hahlbrock K., Saedler H., Weisshaar B.
Proc. Natl. Acad. Sci. U.S.A. 95:12432-12437(1998) [PubMed: 9770503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Columbia and cv. Landsberg erecta.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

U72631 Genomic DNA. Translation: AAB17393.1.
U84258 Genomic DNA. Translation: AAC69362.1.
U84259 mRNA. Translation: AAC69363.1.
U84260 mRNA. Translation: AAB41504.1.
AB006697 Genomic DNA. Translation: BAB10013.1.
AY058068 mRNA. Translation: AAL24176.1.
BT000494 mRNA. Translation: AAN18063.1.
AY086328 mRNA. Translation: AAM64397.1.
IPIIPI00532962.
RefSeqNP_196481.1.
UniGeneAt.8771

3D structure databases

HSSPHSSP built from PDB template 1GP6 based on UniProtKB Q96323.
ModBaseSearch...

Protein-protein interaction databases

IntActQ96330. 3 interactions.

Proteomic databases

PRIDEQ96330.
ProMEXQ96330.

Genome annotation databases

GeneID830765.
GenomeReviewsGene locus AT5G08640 in contig BA000015_GR.
KEGGath:AT5G08640.
NMPDRfig|3702.1.peg.23017.

Organism-specific databases

TAIRAt5g08640.

Phylogenomic databases

OMAQ96330. REVNEEY.

Enzyme and pathway databases

BRENDA1.14.11.23. 302.
1.14.11.9. 302.

Gene expression databases

ArrayExpressQ96330.
GermOnlineAT5G08640. Arabidopsis thaliana.

Family and domain databases

InterProIPR005123. Oxoglutarate/Fe-dep_Oase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFLS1_ARATH
AccessionPrimary (citable) accession number: Q96330
Secondary accession number(s): O04730 expand/collapse secondary AC list , O04731, O04732, O04830, O04831, O04832
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents