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Protein

Acyl-coenzyme A oxidase 4, peroxisomal

Gene

ACX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the desaturation of short-chain acyl-CoAs to 2-trans-enoyl-CoAs. Active on butyryl-CoA (C4), hexanoyl-CoA (C6), and octanoyl-CoA (C8). Has no activity as acyl-CoA dehydrogenase or on crotonyl-CoA (an unsaturated C4:1 carbocyclic ester) or glutaryl-CoA (a dicarboxylic ester).

Catalytic activityi

Acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2.

Cofactori

Kineticsi

  1. KM=8.3 µM for hexanoyl-CoA

    pH dependencei

    Optimum pH is 8.5-9.0.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei181Substrate; via carbonyl oxygenBy similarity1
    Binding sitei288Substrate1
    Binding sitei313FAD1
    Binding sitei409Substrate; via amide nitrogen1
    Binding sitei420Substrate1
    Binding sitei428FAD; via carbonyl oxygen1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi172 – 181FAD10
    Nucleotide bindingi205 – 207FAD3
    Nucleotide bindingi324 – 326FAD3
    Nucleotide bindingi381 – 385FAD5
    Nucleotide bindingi410 – 412FAD3

    GO - Molecular functioni

    GO - Biological processi

    • embryo development ending in seed dormancy Source: TAIR
    • fatty acid beta-oxidation Source: TAIR
    • fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: GO_Central
    • lipid homeostasis Source: GO_Central
    • short-chain fatty acid metabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT3G51840-MONOMER.
    MetaCyc:AT3G51840-MONOMER.
    BRENDAi1.3.3.6. 399.
    ReactomeiR-ATH-71064. Lysine catabolism.

    Chemistry databases

    SwissLipidsiSLP:000000862.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A oxidase 4, peroxisomal (EC:1.3.3.6)
    Short name:
    AOX 4
    Alternative name(s):
    G6p
    Short-chain acyl-CoA oxidase
    Short name:
    AtCX4
    Short name:
    AtG6
    Short name:
    SAOX
    Gene namesi
    Name:ACX4
    Synonyms:G6
    Ordered Locus Names:At3g51840
    ORF Names:ATEM1.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G51840.

    Subcellular locationi

    GO - Cellular componenti

    • glyoxysome Source: UniProtKB-SubCell
    • peroxisome Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00002012112 – 436Acyl-coenzyme A oxidase 4, peroxisomalAdd BLAST435

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylalanineCombined sources1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ96329.
    PRIDEiQ96329.

    PTM databases

    iPTMnetiQ96329.

    Expressioni

    Tissue specificityi

    Particularly abundant in etiolated cotyledons. Also present in flowers, roots and siliques, but not detected in green cotyledons, rosette leaves and stems.1 Publication

    Developmental stagei

    Induced by seed imbibition with a peak at day 5 to 7. Decreases after illumination but still detectable 5 days after illumination.

    Inductioni

    Induced by dehydration and abscisic acid (ABA).1 Publication

    Gene expression databases

    GenevisibleiQ96329. AT.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi9665. 1 interactor.
    STRINGi3702.AT3G51840.1.

    Structurei

    Secondary structure

    1436
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi19 – 23Combined sources5
    Helixi28 – 31Combined sources4
    Helixi51 – 54Combined sources4
    Helixi57 – 73Combined sources17
    Helixi75 – 84Combined sources10
    Helixi89 – 91Combined sources3
    Helixi92 – 96Combined sources5
    Turni97 – 99Combined sources3
    Turni101 – 104Combined sources4
    Helixi115 – 128Combined sources14
    Helixi130 – 140Combined sources11
    Helixi143 – 150Combined sources8
    Helixi153 – 164Combined sources12
    Beta strandi170 – 173Combined sources4
    Beta strandi179 – 181Combined sources3
    Helixi183 – 185Combined sources3
    Beta strandi189 – 193Combined sources5
    Beta strandi196 – 207Combined sources12
    Turni208 – 211Combined sources4
    Beta strandi213 – 221Combined sources9
    Turni222 – 224Combined sources3
    Beta strandi225 – 233Combined sources9
    Beta strandi239 – 243Combined sources5
    Beta strandi247 – 250Combined sources4
    Beta strandi255 – 266Combined sources12
    Helixi267 – 269Combined sources3
    Helixi277 – 312Combined sources36
    Helixi320 – 322Combined sources3
    Helixi324 – 352Combined sources29
    Helixi358 – 382Combined sources25
    Helixi383 – 388Combined sources6
    Helixi390 – 392Combined sources3
    Helixi394 – 406Combined sources13
    Beta strandi407 – 409Combined sources3
    Helixi411 – 423Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2IX5X-ray2.70A/B/C/D1-436[»]
    2IX6X-ray3.90A/B/C/D/E/F1-436[»]
    ProteinModelPortaliQ96329.
    SMRiQ96329.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96329.

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi434 – 436Microbody targeting signalSequence analysis3

    Sequence similaritiesi

    Belongs to the acyl-CoA dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiKOG0138. Eukaryota.
    COG1960. LUCA.
    HOGENOMiHOG000131662.
    InParanoidiQ96329.
    KOiK00232.
    OMAiYQFAQDK.
    OrthoDBiEOG09360BI1.
    PhylomeDBiQ96329.

    Family and domain databases

    Gene3Di1.10.540.10. 1 hit.
    InterProiIPR006089. Acyl-CoA_DH_CS.
    IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
    IPR009075. AcylCo_DH/oxidase_C.
    IPR013786. AcylCoA_DH/ox_N.
    IPR009100. AcylCoA_DH/oxidase_NM_dom.
    [Graphical view]
    PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
    PF02770. Acyl-CoA_dh_M. 1 hit.
    PF02771. Acyl-CoA_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47203. SSF47203. 1 hit.
    SSF56645. SSF56645. 1 hit.
    PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
    PS00073. ACYL_COA_DH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96329-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVLSSADRA SNEKKVKSSY FDLPPMEMSV AFPQATPAST FPPCTSDYYH
    60 70 80 90 100
    FNDLLTPEEQ AIRKKVRECM EKEVAPIMTE YWEKAEFPFH ITPKLGAMGV
    110 120 130 140 150
    AGGSIKGYGC PGLSITANAI ATAEIARVDA SCSTFILVHS SLGMLTIALC
    160 170 180 190 200
    GSEAQKEKYL PSLAQLNTVA CWALTEPDNG SDASGLGTTA TKVEGGWKIN
    210 220 230 240 250
    GQKRWIGNST FADLLIIFAR NTTTNQINGF IVKKDAPGLK ATKIPNKIGL
    260 270 280 290 300
    RMVQNGDILL QNVFVPDEDR LPGVNSFQDT SKVLAVSRVM VAWQPIGISM
    310 320 330 340 350
    GIYDMCHRYL KERKQFGAPL AAFQLNQQKL VQMLGNVQAM FLMGWRLCKL
    360 370 380 390 400
    YETGQMTPGQ ASLGKAWISS KARETASLGR ELLGGNGILA DFLVAKAFCD
    410 420 430
    LEPIYTYEGT YDINTLVTGR EVTGIASFKP ATRSRL
    Length:436
    Mass (Da):47,557
    Last modified:February 1, 1997 - v1
    Checksum:iB6050262D9482C6D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U72505 mRNA. Translation: AAB18129.1.
    AB017643 mRNA. Translation: BAA82478.1.
    AF049236 Genomic DNA. Translation: AAC14411.1.
    CP002686 Genomic DNA. Translation: AEE78851.1.
    AY094441 mRNA. Translation: AAM19813.1.
    AY125536 mRNA. Translation: AAM78046.1.
    AY087793 mRNA. Translation: AAM65329.1.
    PIRiT46895.
    RefSeqiNP_190752.1. NM_115043.3.
    UniGeneiAt.21620.

    Genome annotation databases

    EnsemblPlantsiAT3G51840.1; AT3G51840.1; AT3G51840.
    GeneIDi824347.
    GrameneiAT3G51840.1; AT3G51840.1; AT3G51840.
    KEGGiath:AT3G51840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U72505 mRNA. Translation: AAB18129.1.
    AB017643 mRNA. Translation: BAA82478.1.
    AF049236 Genomic DNA. Translation: AAC14411.1.
    CP002686 Genomic DNA. Translation: AEE78851.1.
    AY094441 mRNA. Translation: AAM19813.1.
    AY125536 mRNA. Translation: AAM78046.1.
    AY087793 mRNA. Translation: AAM65329.1.
    PIRiT46895.
    RefSeqiNP_190752.1. NM_115043.3.
    UniGeneiAt.21620.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2IX5X-ray2.70A/B/C/D1-436[»]
    2IX6X-ray3.90A/B/C/D/E/F1-436[»]
    ProteinModelPortaliQ96329.
    SMRiQ96329.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi9665. 1 interactor.
    STRINGi3702.AT3G51840.1.

    Chemistry databases

    SwissLipidsiSLP:000000862.

    PTM databases

    iPTMnetiQ96329.

    Proteomic databases

    PaxDbiQ96329.
    PRIDEiQ96329.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G51840.1; AT3G51840.1; AT3G51840.
    GeneIDi824347.
    GrameneiAT3G51840.1; AT3G51840.1; AT3G51840.
    KEGGiath:AT3G51840.

    Organism-specific databases

    TAIRiAT3G51840.

    Phylogenomic databases

    eggNOGiKOG0138. Eukaryota.
    COG1960. LUCA.
    HOGENOMiHOG000131662.
    InParanoidiQ96329.
    KOiK00232.
    OMAiYQFAQDK.
    OrthoDBiEOG09360BI1.
    PhylomeDBiQ96329.

    Enzyme and pathway databases

    BioCyciARA:AT3G51840-MONOMER.
    MetaCyc:AT3G51840-MONOMER.
    BRENDAi1.3.3.6. 399.
    ReactomeiR-ATH-71064. Lysine catabolism.

    Miscellaneous databases

    EvolutionaryTraceiQ96329.
    PROiQ96329.

    Gene expression databases

    GenevisibleiQ96329. AT.

    Family and domain databases

    Gene3Di1.10.540.10. 1 hit.
    InterProiIPR006089. Acyl-CoA_DH_CS.
    IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
    IPR009075. AcylCo_DH/oxidase_C.
    IPR013786. AcylCoA_DH/ox_N.
    IPR009100. AcylCoA_DH/oxidase_NM_dom.
    [Graphical view]
    PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
    PF02770. Acyl-CoA_dh_M. 1 hit.
    PF02771. Acyl-CoA_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47203. SSF47203. 1 hit.
    SSF56645. SSF56645. 1 hit.
    PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
    PS00073. ACYL_COA_DH_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiACOX4_ARATH
    AccessioniPrimary (citable) accession number: Q96329
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: February 1, 1997
    Last modified: November 30, 2016
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.