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Protein

Acyl-coenzyme A oxidase 4, peroxisomal

Gene

ACX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the desaturation of short-chain acyl-CoAs to 2-trans-enoyl-CoAs. Active on butyryl-CoA (C4), hexanoyl-CoA (C6), and octanoyl-CoA (C8). Has no activity as acyl-CoA dehydrogenase or on crotonyl-CoA (an unsaturated C4:1 carbocyclic ester) or glutaryl-CoA (a dicarboxylic ester).

Catalytic activityi

Acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2.

Cofactori

Kineticsi

  1. KM=8.3 µM for hexanoyl-CoA

    pH dependencei

    Optimum pH is 8.5-9.0.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei181Substrate; via carbonyl oxygenBy similarity1
    Binding sitei288Substrate1
    Binding sitei313FAD1
    Binding sitei409Substrate; via amide nitrogen1
    Binding sitei420Substrate1
    Binding sitei428FAD; via carbonyl oxygen1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi172 – 181FAD10
    Nucleotide bindingi205 – 207FAD3
    Nucleotide bindingi324 – 326FAD3
    Nucleotide bindingi381 – 385FAD5
    Nucleotide bindingi410 – 412FAD3

    GO - Molecular functioni

    GO - Biological processi

    • embryo development ending in seed dormancy Source: TAIR
    • fatty acid beta-oxidation Source: TAIR
    • short-chain fatty acid metabolic process Source: TAIR

    Keywordsi

    Molecular functionOxidoreductase
    Biological processFatty acid metabolism, Lipid metabolism
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT3G51840-MONOMER
    MetaCyc:AT3G51840-MONOMER
    BRENDAi1.3.3.6 399
    ReactomeiR-ATH-71064 Lysine catabolism

    Chemistry databases

    SwissLipidsiSLP:000000862

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A oxidase 4, peroxisomal (EC:1.3.3.6)
    Short name:
    AOX 4
    Alternative name(s):
    G6p
    Short-chain acyl-CoA oxidase
    Short name:
    AtCX4
    Short name:
    AtG6
    Short name:
    SAOX
    Gene namesi
    Name:ACX4
    Synonyms:G6
    Ordered Locus Names:At3g51840
    ORF Names:ATEM1.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi

    Organism-specific databases

    AraportiAT3G51840
    TAIRilocus:2074403 AT3G51840

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00002012112 – 436Acyl-coenzyme A oxidase 4, peroxisomalAdd BLAST435

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylalanineCombined sources1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ96329
    PRIDEiQ96329

    PTM databases

    iPTMnetiQ96329

    Expressioni

    Tissue specificityi

    Particularly abundant in etiolated cotyledons. Also present in flowers, roots and siliques, but not detected in green cotyledons, rosette leaves and stems.1 Publication

    Developmental stagei

    Induced by seed imbibition with a peak at day 5 to 7. Decreases after illumination but still detectable 5 days after illumination.

    Inductioni

    Induced by dehydration and abscisic acid (ABA).1 Publication

    Gene expression databases

    ExpressionAtlasiQ96329 baseline and differential
    GenevisibleiQ96329 AT

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi9665, 1 interactor
    STRINGi3702.AT3G51840.1

    Structurei

    Secondary structure

    1436
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi19 – 23Combined sources5
    Helixi28 – 31Combined sources4
    Helixi51 – 54Combined sources4
    Helixi57 – 73Combined sources17
    Helixi75 – 84Combined sources10
    Helixi89 – 91Combined sources3
    Helixi92 – 96Combined sources5
    Turni97 – 99Combined sources3
    Turni101 – 104Combined sources4
    Helixi115 – 128Combined sources14
    Helixi130 – 140Combined sources11
    Helixi143 – 150Combined sources8
    Helixi153 – 164Combined sources12
    Beta strandi170 – 173Combined sources4
    Beta strandi179 – 181Combined sources3
    Helixi183 – 185Combined sources3
    Beta strandi189 – 193Combined sources5
    Beta strandi196 – 207Combined sources12
    Turni208 – 211Combined sources4
    Beta strandi213 – 221Combined sources9
    Turni222 – 224Combined sources3
    Beta strandi225 – 233Combined sources9
    Beta strandi239 – 243Combined sources5
    Beta strandi247 – 250Combined sources4
    Beta strandi255 – 266Combined sources12
    Helixi267 – 269Combined sources3
    Helixi277 – 312Combined sources36
    Helixi320 – 322Combined sources3
    Helixi324 – 352Combined sources29
    Helixi358 – 382Combined sources25
    Helixi383 – 388Combined sources6
    Helixi390 – 392Combined sources3
    Helixi394 – 406Combined sources13
    Beta strandi407 – 409Combined sources3
    Helixi411 – 423Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2IX5X-ray2.70A/B/C/D1-436[»]
    2IX6X-ray3.90A/B/C/D/E/F1-436[»]
    ProteinModelPortaliQ96329
    SMRiQ96329
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96329

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi434 – 436Microbody targeting signalSequence analysis3

    Sequence similaritiesi

    Belongs to the acyl-CoA dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiKOG0138 Eukaryota
    COG1960 LUCA
    HOGENOMiHOG000131662
    InParanoidiQ96329
    KOiK00232
    OMAiTQTYEGQ
    OrthoDBiEOG09360BI1
    PhylomeDBiQ96329

    Family and domain databases

    Gene3Di1.10.540.10, 1 hit
    InterProiView protein in InterPro
    IPR006089 Acyl-CoA_DH_CS
    IPR006091 Acyl-CoA_Oxase/DH_cen-dom
    IPR036250 AcylCo_DH-like_C
    IPR009075 AcylCo_DH/oxidase_C
    IPR013786 AcylCoA_DH/ox_N
    IPR037069 AcylCoA_DH/ox_N_sf
    IPR009100 AcylCoA_DH/oxidase_NM_dom
    PfamiView protein in Pfam
    PF00441 Acyl-CoA_dh_1, 1 hit
    PF02770 Acyl-CoA_dh_M, 1 hit
    PF02771 Acyl-CoA_dh_N, 1 hit
    SUPFAMiSSF47203 SSF47203, 1 hit
    SSF56645 SSF56645, 1 hit
    PROSITEiView protein in PROSITE
    PS00072 ACYL_COA_DH_1, 1 hit
    PS00073 ACYL_COA_DH_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96329-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVLSSADRA SNEKKVKSSY FDLPPMEMSV AFPQATPAST FPPCTSDYYH
    60 70 80 90 100
    FNDLLTPEEQ AIRKKVRECM EKEVAPIMTE YWEKAEFPFH ITPKLGAMGV
    110 120 130 140 150
    AGGSIKGYGC PGLSITANAI ATAEIARVDA SCSTFILVHS SLGMLTIALC
    160 170 180 190 200
    GSEAQKEKYL PSLAQLNTVA CWALTEPDNG SDASGLGTTA TKVEGGWKIN
    210 220 230 240 250
    GQKRWIGNST FADLLIIFAR NTTTNQINGF IVKKDAPGLK ATKIPNKIGL
    260 270 280 290 300
    RMVQNGDILL QNVFVPDEDR LPGVNSFQDT SKVLAVSRVM VAWQPIGISM
    310 320 330 340 350
    GIYDMCHRYL KERKQFGAPL AAFQLNQQKL VQMLGNVQAM FLMGWRLCKL
    360 370 380 390 400
    YETGQMTPGQ ASLGKAWISS KARETASLGR ELLGGNGILA DFLVAKAFCD
    410 420 430
    LEPIYTYEGT YDINTLVTGR EVTGIASFKP ATRSRL
    Length:436
    Mass (Da):47,557
    Last modified:February 1, 1997 - v1
    Checksum:iB6050262D9482C6D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U72505 mRNA Translation: AAB18129.1
    AB017643 mRNA Translation: BAA82478.1
    AF049236 Genomic DNA Translation: AAC14411.1
    CP002686 Genomic DNA Translation: AEE78851.1
    AY094441 mRNA Translation: AAM19813.1
    AY125536 mRNA Translation: AAM78046.1
    AY087793 mRNA Translation: AAM65329.1
    PIRiT46895
    RefSeqiNP_190752.1, NM_115043.3
    UniGeneiAt.21620

    Genome annotation databases

    EnsemblPlantsiAT3G51840.1; AT3G51840.1; AT3G51840
    GeneIDi824347
    GrameneiAT3G51840.1; AT3G51840.1; AT3G51840
    KEGGiath:AT3G51840

    Similar proteinsi

    Entry informationi

    Entry nameiACOX4_ARATH
    AccessioniPrimary (citable) accession number: Q96329
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: February 1, 1997
    Last modified: April 25, 2018
    This is version 133 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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