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Protein

Leucoanthocyanidin dioxygenase

Gene

LDOX

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in anthocyanin and protoanthocyanidin biosynthesis by catalyzing the oxidation of leucoanthocyanidins into anthocyanidins. Possesses low flavonol synthase activity in vitro towards dihydrokaempferol and dihydroquercetin producing kaempferol and quercitin, respectively.5 Publications

Catalytic activityi

Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142Substrate2 Publications1
Binding sitei213Substrate2 Publications1
Metal bindingi232Iron; catalytic2 Publications1
Binding sitei233Substrate2 Publications1
Metal bindingi234Iron; catalytic2 Publications1
Metal bindingi288Iron; catalytic2 Publications1
Binding sitei306Substrate2 Publications1
Binding sitei341Substrate2 Publications1

GO - Molecular functioni

  • dioxygenase activity Source: GO_Central
  • L-ascorbic acid binding Source: UniProtKB-KW
  • leucocyanidin oxygenase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • anthocyanin-containing compound biosynthetic process Source: TAIR
  • proanthocyanidin biosynthetic process Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: TAIR
  • vacuole organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BioCyciARA:AT4G22880-MONOMER.
MetaCyc:AT4G22880-MONOMER.
UniPathwayiUPA00009.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucoanthocyanidin dioxygenase (EC:1.14.11.19)
Short name:
LDOX
Short name:
Leucocyanidin oxygenase
Alternative name(s):
Anthocyanidin synthase
Short name:
ANS
Leucoanthocyanidin hydroxylase
Protein TANNIN DEFICIENT SEED 4
Short name:
TDS4
Protein TRANSPARENT TESTA 11
Short name:
TT11
Protein TRANSPARENT TESTA 17
Short name:
TT17
Protein TRANSPARENT TESTA 18
Short name:
TT18
Gene namesi
Name:LDOX
Ordered Locus Names:At4g22880
ORF Names:F7H19.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G22880.

Pathology & Biotechi

Disruption phenotypei

No accumulation of anthocyanins, accumulation of protoanthocyanidin intermediates and presence of numerous small vacuoles in leaf epidermal cells.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78G → E in tt11-2; no accumulation of anthocyanin. 1 Publication1
Mutagenesisi128K → A: Retains two-third of the original activity. 1 Publication1
Mutagenesisi131N → A or D: Retains two-third of the original activity. 1 Publication1
Mutagenesisi142Y → H: Retains two-third of the original activity. 1 Publication1
Mutagenesisi220C → Y in tt17; no accumulation of anthocyanin. 1 Publication1
Mutagenesisi228G → D in tds4-1; no accumulation of anthocyanin. 2 Publications1
Mutagenesisi230E → Q: Retains one half of the original activity. 1 Publication1
Mutagenesisi341K → N: Retains two-third of the original activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000672991 – 356Leucoanthocyanidin dioxygenaseAdd BLAST356

Proteomic databases

PaxDbiQ96323.

PTM databases

iPTMnetiQ96323.

Expressioni

Tissue specificityi

Expressed in young seedlings (at protein level).1 Publication

Inductioni

By methyl jasmonate, 6-benzylaminopurine, light and sucrose.3 Publications

Gene expression databases

ExpressionAtlasiQ96323. baseline and differential.
GenevisibleiQ96323. AT.

Interactioni

Protein-protein interaction databases

BioGridi13676. 1 interactor.
STRINGi3702.AT4G22880.1.

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6
Helixi20 – 22Combined sources3
Helixi26 – 29Combined sources4
Helixi35 – 40Combined sources6
Beta strandi49 – 51Combined sources3
Turni53 – 56Combined sources4
Helixi60 – 76Combined sources17
Beta strandi78 – 84Combined sources7
Helixi89 – 103Combined sources15
Helixi107 – 110Combined sources4
Helixi111 – 113Combined sources3
Helixi117 – 119Combined sources3
Beta strandi123 – 126Combined sources4
Beta strandi132 – 134Combined sources3
Beta strandi141 – 149Combined sources9
Helixi150 – 152Combined sources3
Helixi155 – 157Combined sources3
Helixi165 – 190Combined sources26
Helixi197 – 201Combined sources5
Helixi204 – 207Combined sources4
Beta strandi209 – 217Combined sources9
Turni223 – 225Combined sources3
Beta strandi228 – 232Combined sources5
Beta strandi236 – 243Combined sources8
Beta strandi249 – 253Combined sources5
Beta strandi256 – 259Combined sources4
Beta strandi267 – 271Combined sources5
Helixi273 – 278Combined sources6
Turni279 – 281Combined sources3
Beta strandi288 – 290Combined sources3
Beta strandi298 – 306Combined sources9
Turni309 – 311Combined sources3
Helixi318 – 320Combined sources3
Beta strandi323 – 325Combined sources3
Helixi334 – 349Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GP4X-ray2.10A1-356[»]
1GP5X-ray2.20A1-356[»]
1GP6X-ray1.75A1-356[»]
2BRTX-ray2.20A2-356[»]
ProteinModelPortaliQ96323.
SMRiQ96323.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96323.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 307Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 2172-oxoglutarate binding2 Publications3
Regioni298 – 3002-oxoglutarate binding2 Publications3

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
InParanoidiQ96323.
KOiK05277.
OMAiACGQLEW.
OrthoDBiEOG09360F9L.
PhylomeDBiQ96323.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAVERVESL AKSGIISIPK EYIRPKEELE SINDVFLEEK KEDGPQVPTI
60 70 80 90 100
DLKNIESDDE KIRENCIEEL KKASLDWGVM HLINHGIPAD LMERVKKAGE
110 120 130 140 150
EFFSLSVEEK EKYANDQATG KIQGYGSKLA NNASGQLEWE DYFFHLAYPE
160 170 180 190 200
EKRDLSIWPK TPSDYIEATS EYAKCLRLLA TKVFKALSVG LGLEPDRLEK
210 220 230 240 250
EVGGLEELLL QMKINYYPKC PQPELALGVE AHTDVSALTF ILHNMVPGLQ
260 270 280 290 300
LFYEGKWVTA KCVPDSIVMH IGDTLEILSN GKYKSILHRG LVNKEKVRIS
310 320 330 340 350
WAVFCEPPKD KIVLKPLPEM VSVESPAKFP PRTFAQHIEH KLFGKEQEEL

VSEKND
Length:356
Mass (Da):40,396
Last modified:February 1, 1997 - v1
Checksum:i1B74AE3A54056201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70478 mRNA. Translation: AAB09572.1.
AL031018 Genomic DNA. Translation: CAA19803.1.
AL161558 Genomic DNA. Translation: CAB79243.1.
CP002687 Genomic DNA. Translation: AEE84672.1.
CP002687 Genomic DNA. Translation: AEE84673.1.
AY088203 mRNA. Translation: AAM65745.1.
PIRiT05119.
RefSeqiNP_001031700.1. NM_001036623.1.
NP_194019.1. NM_118417.2.
UniGeneiAt.2369.

Genome annotation databases

EnsemblPlantsiAT4G22880.1; AT4G22880.1; AT4G22880.
AT4G22880.2; AT4G22880.2; AT4G22880.
GeneIDi828387.
GrameneiAT4G22880.1; AT4G22880.1; AT4G22880.
AT4G22880.2; AT4G22880.2; AT4G22880.
KEGGiath:AT4G22880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70478 mRNA. Translation: AAB09572.1.
AL031018 Genomic DNA. Translation: CAA19803.1.
AL161558 Genomic DNA. Translation: CAB79243.1.
CP002687 Genomic DNA. Translation: AEE84672.1.
CP002687 Genomic DNA. Translation: AEE84673.1.
AY088203 mRNA. Translation: AAM65745.1.
PIRiT05119.
RefSeqiNP_001031700.1. NM_001036623.1.
NP_194019.1. NM_118417.2.
UniGeneiAt.2369.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GP4X-ray2.10A1-356[»]
1GP5X-ray2.20A1-356[»]
1GP6X-ray1.75A1-356[»]
2BRTX-ray2.20A2-356[»]
ProteinModelPortaliQ96323.
SMRiQ96323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13676. 1 interactor.
STRINGi3702.AT4G22880.1.

PTM databases

iPTMnetiQ96323.

Proteomic databases

PaxDbiQ96323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G22880.1; AT4G22880.1; AT4G22880.
AT4G22880.2; AT4G22880.2; AT4G22880.
GeneIDi828387.
GrameneiAT4G22880.1; AT4G22880.1; AT4G22880.
AT4G22880.2; AT4G22880.2; AT4G22880.
KEGGiath:AT4G22880.

Organism-specific databases

TAIRiAT4G22880.

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
InParanoidiQ96323.
KOiK05277.
OMAiACGQLEW.
OrthoDBiEOG09360F9L.
PhylomeDBiQ96323.

Enzyme and pathway databases

UniPathwayiUPA00009.
BioCyciARA:AT4G22880-MONOMER.
MetaCyc:AT4G22880-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ96323.
PROiQ96323.

Gene expression databases

ExpressionAtlasiQ96323. baseline and differential.
GenevisibleiQ96323. AT.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDOX_ARATH
AccessioniPrimary (citable) accession number: Q96323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.