Reviewed, UniProtKB/Swiss-Prot Q96323 (LDOX_ARATH)

Names and origin

Protein names

Recommended name:
    Leucoanthocyanidin dioxygenase
      Short name=LDOX
      Short name=Leucocyanidin oxygenase
    EC=1.14.11.19
Alternative name(s):
    Leucoanthocyanidin hydroxylase
    Anthocyanidin synthase
      Short name=ANS

Gene names

Name:	LDOX
Ordered Locus Names:	At4g22880
ORF Names:	F7H19.60

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidation of leucoanthocyanidins into anthocyanidins.
Catalytic activity	Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O.
Cofactor	Binds 1 ascorbate molecule per subunit. Binds 1 iron ion per subunit.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	anthocyanin biosynthetic process Traceable author statement. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proanthocyanidin biosynthetic process Inferred from mutant phenotype. Source: TAIR response to jasmonic acid stimulus Inferred from expression pattern. Source: TAIR response to wounding Inferred from expression pattern. Source: TAIR vacuole organization Inferred from mutant phenotype. Source: TAIR
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW leucocyanidin oxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 356

356

Leucoanthocyanidin dioxygenase

PRO_0000067299

Regions

Domain

208 – 307

100

Fe2OG dioxygenase

Sites

Metal binding

232

1

Iron

Metal binding

234

1

Iron

Metal binding

288

1

Iron

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

356

Helix Strand Turn

Details...

Helix

7 – 11

5

Turn

12 – 14

3

Helix

20 – 22

3

Helix

26 – 29

4

Helix

35 – 40

6

Beta strand

49 – 51

3

Turn

53 – 56

4

Helix

60 – 76

17

Beta strand

78 – 84

7

Helix

89 – 103

15

Helix

107 – 110

4

Helix

111 – 113

3

Helix

117 – 119

3

Beta strand

124 – 126

3

Beta strand

141 – 149

9

Helix

150 – 152

3

Helix

155 – 157

3

Helix

165 – 190

26

Helix

197 – 201

5

Helix

204 – 207

4

Beta strand

209 – 217

9

Helix

223 – 225

3

Beta strand

228 – 232

5

Beta strand

236 – 243

8

Beta strand

249 – 253

5

Beta strand

256 – 259

4

Beta strand

267 – 271

5

Helix

273 – 278

6

Turn

279 – 281

3

Beta strand

288 – 290

3

Beta strand

298 – 306

9

Turn

309 – 311

3

Helix

318 – 320

3

Beta strand

323 – 325

3

Helix

334 – 346

13

Sequences

	Sequence		Length	Mass (Da)
	Q96323-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1B74AE3A54056201	FASTA	356	40,396
	10 20 30 40 50 60 MVAVERVESL AKSGIISIPK EYIRPKEELE SINDVFLEEK KEDGPQVPTI DLKNIESDDE 70 80 90 100 110 120 KIRENCIEEL KKASLDWGVM HLINHGIPAD LMERVKKAGE EFFSLSVEEK EKYANDQATG 130 140 150 160 170 180 KIQGYGSKLA NNASGQLEWE DYFFHLAYPE EKRDLSIWPK TPSDYIEATS EYAKCLRLLA 190 200 210 220 230 240 TKVFKALSVG LGLEPDRLEK EVGGLEELLL QMKINYYPKC PQPELALGVE AHTDVSALTF 250 260 270 280 290 300 ILHNMVPGLQ LFYEGKWVTA KCVPDSIVMH IGDTLEILSN GKYKSILHRG LVNKEKVRIS 310 320 330 340 350 WAVFCEPPKD KIVLKPLPEM VSVESPAKFP PRTFAQHIEH KLFGKEQEEL VSEKND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of flavonol synthase and leucoanthocyanidin dioxygenase genes in Arabidopsis. Further evidence for differential regulation of 'early' and 'late' genes." Pelletier M.K., Murrell J.R., Shirley B.W. Plant Physiol. 113:1437-1445(1997) [PubMed: 9112784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana." Wilmouth R.C., Turnbull J.J., Welford R.W., Clifton I.J., Prescott A.G., Schofield C.J. Structure 10:93-103(2002) [PubMed: 11796114] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U70478 mRNA. Translation: AAB09572.1.
AL031018 Genomic DNA. Translation: CAA19803.1.
AL161558 Genomic DNA. Translation: CAB79243.1.
AY088203 mRNA. Translation: AAM65745.1.

IPI

IPI00525167.

PIR

T05119.

RefSeq

NP_001031700.1.
NP_194019.1.

UniGene

At.2369
Rra.2938
Rsa.1075

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GP4	X-ray	2.10	A	1-356	[»]
1GP5	X-ray	2.20	A	1-356	[»]
1GP6	X-ray	1.75	A	1-356	[»]
2BRT	X-ray	2.20	A	2-356	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q96323.

Proteomic databases

PRIDE

Q96323.

Genome annotation databases

GeneID

828387.

GenomeReviews

Gene locus AT4G22880 in contig CT486007_GR.

KEGG

ath:AT4G22880.

NMPDR

fig|3702.1.peg.20150.

Organism-specific databases

TAIR

At4g22880.

Phylogenomic databases

HOGENOM

HBG749762.

InParanoid

Q96323.

OMA

VPTIDLK.

Enzyme and pathway databases

BRENDA

1.14.11.19. 302.

Gene expression databases

Genevestigator

Q96323.

GermOnline

AT4G22880. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR005123. Oxoglutarate/Fe-dep_oxygenase.
[Graphical view]

Pfam

PF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]

PROSITE

PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LDOX_ARATH

Accession

Primary (citable) accession number: Q96323

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	February 1, 1997
Last modified:	February 9, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)