Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY

Gene

SPY

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Functions with GIGANTEA (GI) in pathways controlling flowering, circadian cotyledon movements and hypocotyl elongation. Acts as a light-regulated promoter of elongation via its interaction with GI. Acts as an activator of cytokinin signaling. Required with SEC for gamete and seed development. Its OGT activity has been proved in vitro but not in vivo.5 Publications

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine.1 Publication
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • protein N-acetylglucosaminyltransferase activity Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cytokinin-activated signaling pathway Source: TAIR
  • flower development Source: UniProtKB-KW
  • gibberellic acid mediated signaling pathway Source: TAIR
  • negative regulation of gibberellic acid mediated signaling pathway Source: TAIR
  • protein glycosylation Source: UniProtKB-UniPathway
  • regulation of reactive oxygen species metabolic process Source: TAIR
  • rhythmic process Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
Biological processBiological rhythms, Differentiation, Flowering, Gibberellin signaling pathway

Enzyme and pathway databases

BRENDAi2.4.1.255 399
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT41 Glycosyltransferase Family 41

Names & Taxonomyi

Protein namesi
Recommended name:
Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY (EC:2.4.1.2551 Publication)
Gene namesi
Name:SPY
Ordered Locus Names:At3g11540
ORF Names:F24K9.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G11540
TAIRilocus:2080722 AT3G11540

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi593G → S in spy-3; induces a constitutive induction of GA signal. 1 Publication1
Mutagenesisi845C → Y in spy-5; induces a constitutive induction of GA signal. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917761 – 914Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLYAdd BLAST914

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96301
PRIDEiQ96301

PTM databases

iPTMnetiQ96301

Expressioni

Tissue specificityi

Widely expressed. Present throughout the plant (at protein level).1 Publication

Developmental stagei

Detected in all organs of the plant and at all stages of the life cycle. Detected 1 day after germination in the radicle just before its emergence from the seed. At 2.5 and 3 days after germination, expression in the young seedling is highest in the cotyledons and the root tip. At 3, 4 and 5 days, expression is also detectable in the hypocotyl. At 10 days of age, expression in the first pair of true leaves is reduced relative to the rest of the seedling. 2 days later, this difference disappears and the expression level is again fairly similar throughout the aboveground portion of the plant, with a higher intensity in the vegetative apex. This developmental regulation is not detected in leaves developing at later nodes. Older plants also display uniform expression throughout the vegetative organs, but this expression is less intense. In older seedlings, expression is observed throughout the root, particularly at the tip of the primary root and in lateral roots. Expression is also observed in trichomes and senescing leaves, and in inflorescence internodes, flowers. Expression is observed in the seeds and carpels of fully elongated siliques. Lower expression is also observed in expanding siliques and in the developing seeds in these siliques. Expression is also detected in the embryo of maturing seeds (after the disappearance of the endosperm) (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ96301 baseline and differential
GenevisibleiQ96301 AT

Interactioni

Subunit structurei

Homomultimer; via its TPR repeats. Interacts with GI.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GIQ9SQI24EBI-446372,EBI-446380

Protein-protein interaction databases

BioGridi5661, 8 interactors
IntActiQ96301, 1 interactor
STRINGi3702.AT3G11540.1

Structurei

3D structure databases

ProteinModelPortaliQ96301
SMRiQ96301
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 76TPR 1Add BLAST34
Repeati77 – 110TPR 2Add BLAST34
Repeati112 – 144TPR 3Add BLAST33
Repeati152 – 185TPR 4Add BLAST34
Repeati186 – 219TPR 5Add BLAST34
Repeati220 – 253TPR 6Add BLAST34
Repeati261 – 294TPR 7Add BLAST34
Repeati295 – 328TPR 8Add BLAST34
Repeati329 – 362TPR 9Add BLAST34
Repeati364 – 396TPR 10Add BLAST33
Repeati397 – 430TPR 11Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni431 – 914Catalytic regionAdd BLAST484

Domaini

The TPR repeats mediate protein-protein interactions and are essential for its function. Expression of such repeats in plants accelerate flowering.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiENOG410IRRE Eukaryota
COG3914 LUCA
HOGENOMiHOG000030819
InParanoidiQ96301
OMAiPLAHHDY
OrthoDBiEOG093601FF
PhylomeDBiQ96301

Family and domain databases

Gene3Di1.25.40.10, 5 hits
InterProiView protein in InterPro
IPR029489 OGT/SEC/SPY_C
IPR006597 Sel1-like
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF13844 Glyco_transf_41, 2 hits
PF00515 TPR_1, 4 hits
SMARTiView protein in SMART
SM00671 SEL1, 4 hits
SM00028 TPR, 11 hits
SUPFAMiSSF48452 SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 11 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q96301-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGLEDDTER ERSPVVENGF SNGSRSSSSS AGVLSPSRKV TQGNDTLSYA
60 70 80 90 100
NILRARNKFA DALALYEAML EKDSKNVEAH IGKGICLQTQ NKGNLAFDCF
110 120 130 140 150
SEAIRLDPHN ACALTHCGIL HKEEGRLVEA AESYQKALMA DASYKPAAEC
160 170 180 190 200
LAIVLTDLGT SLKLAGNTQE GIQKYYEALK IDPHYAPAYY NLGVVYSEMM
210 220 230 240 250
QYDNALSCYE KAALERPMYA EAYCNMGVIY KNRGDLEMAI TCYERCLAVS
260 270 280 290 300
PNFEIAKNNM AIALTDLGTK VKLEGDVTQG VAYYKKALYY NWHYADAMYN
310 320 330 340 350
LGVAYGEMLK FDMAIVFYEL AFHFNPHCAE ACNNLGVLYK DRDNLDKAVE
360 370 380 390 400
CYQMALSIKP NFAQSLNNLG VVYTVQGKMD AAASMIEKAI LANPTYAEAF
410 420 430 440 450
NNLGVLYRDA GNITMAIDAY EECLKIDPDS RNAGQNRLLA MNYINEGLDD
460 470 480 490 500
KLFEAHRDWG WRFTRLHPQY TSWDNLKDPE RPITIGYISP DFFTHSVSYF
510 520 530 540 550
IEAPLTHHDY TKYKVVVYSA VVKADAKTYR FRDKVLKKGG VWKDIYGIDE
560 570 580 590 600
KKIASMVRED KIDILVELTG HTANNKLGTM ACRPAPVQVT WIGYPNTTGL
610 620 630 640 650
PTVDYRITDS LADPPDTKQK QVEELVRLPD CFLCYTPSPE AGPVCPTPAL
660 670 680 690 700
SNGFVTFGSF NNLAKITPKV LQVWARILCA VPNSRLVVKC KPFCCDSIRQ
710 720 730 740 750
RFLTTLEQLG LESKRVDLLP LILFNHDHMQ AYSLMDISLD TFPYAGTTTT
760 770 780 790 800
CESLYMGVPC VTMAGSVHAH NVGVSLLTKV GLGHLVAKNE DEYVQLSVDL
810 820 830 840 850
ASDVTALSKL RMSLRDLMAG SPVCNGPSFA VGLESAYRNM WKKYCKGEVP
860 870 880 890 900
SLRRMEMLQK EVHDDPLISK DLGPSRVSVT GEATPSLKAN GSAPVPSSLP
910
TQSPQLSKRM DSTS
Length:914
Mass (Da):101,430
Last modified:February 1, 1997 - v1
Checksum:iA881D84BB5C33493
GO

Sequence cautioni

The sequence BAD94413 differs from that shown. Reason: Frameshift at position 528.Curated
The sequence BAD95289 differs from that shown. Reason: Frameshift at position 528.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62135 mRNA Translation: AAC49446.1
AC008153 Genomic DNA Translation: AAG51433.1
CP002686 Genomic DNA Translation: AEE75060.1
AK220931 mRNA Translation: BAD94413.1 Frameshift.
AK221192 mRNA Translation: BAD95289.1 Frameshift.
AK221314 mRNA Translation: BAD94086.1
RefSeqiNP_187761.1, NM_111987.4 [Q96301-1]
UniGeneiAt.17656

Genome annotation databases

EnsemblPlantsiAT3G11540.1; AT3G11540.1; AT3G11540 [Q96301-1]
GeneIDi820327
GrameneiAT3G11540.1; AT3G11540.1; AT3G11540 [Q96301-1]
KEGGiath:AT3G11540

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPY_ARATH
AccessioniPrimary (citable) accession number: Q96301
Secondary accession number(s): Q56YX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health