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Protein

Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY

Gene

SPY

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Functions with GIGANTEA (GI) in pathways controlling flowering, circadian cotyledon movements and hypocotyl elongation. Acts as a light-regulated promoter of elongation via its interaction with GI. Acts as an activator of cytokinin signaling. Required with SEC for gamete and seed development. Its OGT activity has been proved in vitro but not in vivo.5 Publications

Catalytic activityi

UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • protein N-acetylglucosaminyltransferase activity Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cytokinin-activated signaling pathway Source: TAIR
  • flower development Source: UniProtKB-KW
  • gibberellic acid mediated signaling pathway Source: TAIR
  • negative regulation of gibberellic acid mediated signaling pathway Source: TAIR
  • protein glycosylation Source: UniProtKB-UniPathway
  • regulation of reactive oxygen species metabolic process Source: TAIR
  • rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Biological processi

Biological rhythms, Differentiation, Flowering, Gibberellin signaling pathway

Enzyme and pathway databases

BioCyciARA:GQT-1691-MONOMER.
ARA:GQT-1692-MONOMER.
BRENDAi2.4.1.255. 399.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT41. Glycosyltransferase Family 41.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY (EC:2.4.1.-)
Gene namesi
Name:SPY
Ordered Locus Names:At3g11540
ORF Names:F24K9.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G11540.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi593 – 5931G → S in spy-3; induces a constitutive induction of GA signal. 1 Publication
Mutagenesisi845 – 8451C → Y in spy-5; induces a constitutive induction of GA signal. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLYPRO_0000191776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96301.
PRIDEiQ96301.

PTM databases

iPTMnetiQ96301.

Expressioni

Tissue specificityi

Widely expressed. Present throughout the plant (at protein level).1 Publication

Developmental stagei

Detected in all organs of the plant and at all stages of the life cycle. Detected 1 day after germination in the radicle just before its emergence from the seed. At 2.5 and 3 days after germination, expression in the young seedling is highest in the cotyledons and the root tip. At 3, 4 and 5 days, expression is also detectable in the hypocotyl. At 10 days of age, expression in the first pair of true leaves is reduced relative to the rest of the seedling. 2 days later, this difference disappears and the expression level is again fairly similar throughout the aboveground portion of the plant, with a higher intensity in the vegetative apex. This developmental regulation is not detected in leaves developing at later nodes. Older plants also display uniform expression throughout the vegetative organs, but this expression is less intense. In older seedlings, expression is observed throughout the root, particularly at the tip of the primary root and in lateral roots. Expression is also observed in trichomes and senescing leaves, and in inflorescence internodes, flowers. Expression is observed in the seeds and carpels of fully elongated siliques. Lower expression is also observed in expanding siliques and in the developing seeds in these siliques. Expression is also detected in the embryo of maturing seeds (after the disappearance of the endosperm) (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ96301. baseline and differential.
GenevisibleiQ96301. AT.

Interactioni

Subunit structurei

Homomultimer; via its TPR repeats. Interacts with GI.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GIQ9SQI24EBI-446372,EBI-446380

Protein-protein interaction databases

BioGridi5661. 8 interactions.
IntActiQ96301. 1 interaction.
STRINGi3702.AT3G11540.1.

Structurei

3D structure databases

ProteinModelPortaliQ96301.
SMRiQ96301. Positions 41-851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati43 – 7634TPR 1Add
BLAST
Repeati77 – 11034TPR 2Add
BLAST
Repeati112 – 14433TPR 3Add
BLAST
Repeati152 – 18534TPR 4Add
BLAST
Repeati186 – 21934TPR 5Add
BLAST
Repeati220 – 25334TPR 6Add
BLAST
Repeati261 – 29434TPR 7Add
BLAST
Repeati295 – 32834TPR 8Add
BLAST
Repeati329 – 36234TPR 9Add
BLAST
Repeati364 – 39633TPR 10Add
BLAST
Repeati397 – 43034TPR 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni431 – 914484Catalytic regionAdd
BLAST

Domaini

The TPR repeats mediate protein-protein interactions and are essential for its function. Expression of such repeats in plants accelerate flowering.1 Publication

Sequence similaritiesi

Contains 11 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiENOG410IRRE. Eukaryota.
COG3914. LUCA.
HOGENOMiHOG000030819.
InParanoidiQ96301.
OMAiYQAHREW.
OrthoDBiEOG093601FF.
PhylomeDBiQ96301.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR029489. OGT/SEC/SPY_C.
IPR006597. Sel1-like.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13844. Glyco_transf_41. 2 hits.
PF00515. TPR_1. 4 hits.
PF13374. TPR_10. 1 hit.
[Graphical view]
SMARTiSM00671. SEL1. 4 hits.
SM00028. TPR. 11 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 11 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q96301-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGLEDDTER ERSPVVENGF SNGSRSSSSS AGVLSPSRKV TQGNDTLSYA
60 70 80 90 100
NILRARNKFA DALALYEAML EKDSKNVEAH IGKGICLQTQ NKGNLAFDCF
110 120 130 140 150
SEAIRLDPHN ACALTHCGIL HKEEGRLVEA AESYQKALMA DASYKPAAEC
160 170 180 190 200
LAIVLTDLGT SLKLAGNTQE GIQKYYEALK IDPHYAPAYY NLGVVYSEMM
210 220 230 240 250
QYDNALSCYE KAALERPMYA EAYCNMGVIY KNRGDLEMAI TCYERCLAVS
260 270 280 290 300
PNFEIAKNNM AIALTDLGTK VKLEGDVTQG VAYYKKALYY NWHYADAMYN
310 320 330 340 350
LGVAYGEMLK FDMAIVFYEL AFHFNPHCAE ACNNLGVLYK DRDNLDKAVE
360 370 380 390 400
CYQMALSIKP NFAQSLNNLG VVYTVQGKMD AAASMIEKAI LANPTYAEAF
410 420 430 440 450
NNLGVLYRDA GNITMAIDAY EECLKIDPDS RNAGQNRLLA MNYINEGLDD
460 470 480 490 500
KLFEAHRDWG WRFTRLHPQY TSWDNLKDPE RPITIGYISP DFFTHSVSYF
510 520 530 540 550
IEAPLTHHDY TKYKVVVYSA VVKADAKTYR FRDKVLKKGG VWKDIYGIDE
560 570 580 590 600
KKIASMVRED KIDILVELTG HTANNKLGTM ACRPAPVQVT WIGYPNTTGL
610 620 630 640 650
PTVDYRITDS LADPPDTKQK QVEELVRLPD CFLCYTPSPE AGPVCPTPAL
660 670 680 690 700
SNGFVTFGSF NNLAKITPKV LQVWARILCA VPNSRLVVKC KPFCCDSIRQ
710 720 730 740 750
RFLTTLEQLG LESKRVDLLP LILFNHDHMQ AYSLMDISLD TFPYAGTTTT
760 770 780 790 800
CESLYMGVPC VTMAGSVHAH NVGVSLLTKV GLGHLVAKNE DEYVQLSVDL
810 820 830 840 850
ASDVTALSKL RMSLRDLMAG SPVCNGPSFA VGLESAYRNM WKKYCKGEVP
860 870 880 890 900
SLRRMEMLQK EVHDDPLISK DLGPSRVSVT GEATPSLKAN GSAPVPSSLP
910
TQSPQLSKRM DSTS
Length:914
Mass (Da):101,430
Last modified:February 1, 1997 - v1
Checksum:iA881D84BB5C33493
GO

Sequence cautioni

The sequence BAD94413 differs from that shown. Reason: Frameshift at position 528. Curated
The sequence BAD95289 differs from that shown. Reason: Frameshift at position 528. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62135 mRNA. Translation: AAC49446.1.
AC008153 Genomic DNA. Translation: AAG51433.1.
CP002686 Genomic DNA. Translation: AEE75060.1.
AK220931 mRNA. Translation: BAD94413.1. Frameshift.
AK221192 mRNA. Translation: BAD95289.1. Frameshift.
AK221314 mRNA. Translation: BAD94086.1.
RefSeqiNP_187761.1. NM_111987.3. [Q96301-1]
UniGeneiAt.17656.

Genome annotation databases

EnsemblPlantsiAT3G11540.1; AT3G11540.1; AT3G11540. [Q96301-1]
GeneIDi820327.
KEGGiath:AT3G11540.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62135 mRNA. Translation: AAC49446.1.
AC008153 Genomic DNA. Translation: AAG51433.1.
CP002686 Genomic DNA. Translation: AEE75060.1.
AK220931 mRNA. Translation: BAD94413.1. Frameshift.
AK221192 mRNA. Translation: BAD95289.1. Frameshift.
AK221314 mRNA. Translation: BAD94086.1.
RefSeqiNP_187761.1. NM_111987.3. [Q96301-1]
UniGeneiAt.17656.

3D structure databases

ProteinModelPortaliQ96301.
SMRiQ96301. Positions 41-851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5661. 8 interactions.
IntActiQ96301. 1 interaction.
STRINGi3702.AT3G11540.1.

Protein family/group databases

CAZyiGT41. Glycosyltransferase Family 41.

PTM databases

iPTMnetiQ96301.

Proteomic databases

PaxDbiQ96301.
PRIDEiQ96301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G11540.1; AT3G11540.1; AT3G11540. [Q96301-1]
GeneIDi820327.
KEGGiath:AT3G11540.

Organism-specific databases

TAIRiAT3G11540.

Phylogenomic databases

eggNOGiENOG410IRRE. Eukaryota.
COG3914. LUCA.
HOGENOMiHOG000030819.
InParanoidiQ96301.
OMAiYQAHREW.
OrthoDBiEOG093601FF.
PhylomeDBiQ96301.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:GQT-1691-MONOMER.
ARA:GQT-1692-MONOMER.
BRENDAi2.4.1.255. 399.

Miscellaneous databases

PROiQ96301.

Gene expression databases

ExpressionAtlasiQ96301. baseline and differential.
GenevisibleiQ96301. AT.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR029489. OGT/SEC/SPY_C.
IPR006597. Sel1-like.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13844. Glyco_transf_41. 2 hits.
PF00515. TPR_1. 4 hits.
PF13374. TPR_10. 1 hit.
[Graphical view]
SMARTiSM00671. SEL1. 4 hits.
SM00028. TPR. 11 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 11 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPY_ARATH
AccessioniPrimary (citable) accession number: Q96301
Secondary accession number(s): Q56YX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.