Q96299 (14339_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3-like protein GF14 mu

Alternative name(s):
General regulatory factor 9

Gene names

Name:	GRF9
Ordered Locus Names:	At2g42590
ORF Names:	F14N22.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q96299-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	14-3-3-like protein GF14 mu		PRO_0000058671
Amino acid modifications
	Modified residue	239	1	Phosphoserine Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: B70A45DF64DFD926	FASTA	263	29,520
	10 20 30 40 50 60 MGSGKERDTF VYLAKLSEQA ERYEEMVESM KSVAKLNVDL TVEERNLLSV GYKNVIGSRR 70 80 90 100 110 120 ASWRIFSSIE QKEAVKGNDV NVKRIKEYME KVELELSNIC IDIMSVLDEH LIPSASEGES 130 140 150 160 170 180 TVFFNKMKGD YYRYLAEFKS GNERKEAADQ SLKAYEIATT AAEAKLPPTH PIRLGLALNF 190 200 210 220 230 240 SVFYYEIMNA PERACHLAKQ AFDEAISELD TLNEESYKDS TLIMQLLRDN LTLWTSDISE 250 260 EGGDDAHKTN GSAKPGAGGD DAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis 14-3-3 multigene family." Wu K., Rooney M.F., Ferl R.J. Plant Physiol. 114:1421-1431(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Members of the Arabidopsis 14-3-3 gene family trans-complement two types of defects in fission yeast." Kuromori T., Yamamoto M. Plant Sci. 158:155-161(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequences of five Arabidopsis general regulatory factor (GRF) genes encoding 14-3-3 proteins." Chung H.-J., Shanker S., Ferl R.J. Plant Gene Register PGR99-114 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana." Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C. J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239, MASS SPECTROMETRY. Strain: cv. Columbia.
[9]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60444 mRNA. Translation: AAB49334.2. AB011545 mRNA. Translation: BAA32735.1. AF145301 Genomic DNA. Translation: AAD51784.1. AC007087 Genomic DNA. Translation: AAD23005.1. CP002685 Genomic DNA. Translation: AEC10142.1. AY093076 mRNA. Translation: AAM13075.1. AY128764 mRNA. Translation: AAM91164.1. AY085927 mRNA. Translation: AAM63139.1.
IPI	IPI00525722.
PIR	T52037.
RefSeq	NP_565977.1. NM_129820.2.
UniGene	At.21859.
3D structure databases
ProteinModelPortal	Q96299.
SMR	Q96299. Positions 6-235.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96299. 4 interactions.
MINT	MINT-145086.
Proteomic databases
PaxDb	Q96299.
PRIDE	Q96299.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G42590.1; AT2G42590.1; AT2G42590.
GeneID	818859.
KEGG	ath:AT2G42590.
Organism-specific databases
TAIR	At2g42590.
Phylogenomic databases
eggNOG	COG5040.
HOGENOM	HOG000240379.
InParanoid	Q96299.
PhylomeDB	Q96299.
ProtClustDB	CLSN2688891.
Gene expression databases
ArrayExpress	Q96299.
Genevestigator	Q96299.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

14339_ARATH

Accession

Primary (citable) accession number: Q96299
Secondary accession number(s): O80367

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program