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Q96293

- ACT8_ARATH

UniProt

Q96293 - ACT8_ARATH

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Protein

Actin-8

Gene

ACT8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins.

Enzyme regulationi

Subject to negative translational control in pollen.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. copper ion binding Source: TAIR

GO - Biological processi

  1. response to salt stress Source: TAIR
  2. root hair cell tip growth Source: TAIR
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_190901. Factors involved in megakaryocyte development and platelet production.
REACT_236222. Prefoldin mediated transfer of substrate to CCT/TriC.
REACT_247245. Folding of actin by CCT/TriC.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-8
Gene namesi
Name:ACT8
Ordered Locus Names:At1g49240
ORF Names:F27J15.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G49240.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytoskeleton Source: UniProtKB-KW
  5. cytosol Source: TAIR
  6. plasma membrane Source: TAIR
  7. plasmodesma Source: TAIR
  8. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Actin-8PRO_0000088893Add
BLAST

Proteomic databases

PaxDbiQ96293.
PRIDEiQ96293.

2D gel databases

SWISS-2DPAGEP99055.

Expressioni

Tissue specificityi

Strongly expressed in nearly all vegetative tissues, and levels remain high in older tissues. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.

Gene expression databases

ExpressionAtlasiQ96293. baseline and differential.
GenevestigatoriQ96293.

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Protein-protein interaction databases

BioGridi26572. 5 interactions.
IntActiQ96293. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ96293.
SMRiQ96293. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiCOG5277.
HOGENOMiHOG000233340.
InParanoidiQ96293.
KOiK10355.
OMAiPPFYRIP.
PhylomeDBiQ96293.

Family and domain databases

InterProiIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96293-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADADDIQPI VCDNGTGMVK AGFAGDDAPR AVFPSVVGRP RHHGVMVGMN
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGVVSN WDDMEKIWHH TFYNELRIAP
110 120 130 140 150
EEHPVLLTEA PLNPKANREK MTQIMFETFN SPAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG FSLPHAILRL DLAGRDLTDY LMKILTERGY
210 220 230 240 250
MFTTTAEREI VRDIKEKLSF VAVDYEQEME TSKTSSSIEK NYELPDGQVI
260 270 280 290 300
TIGAERFRCP EVLFQPSFVG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGTTMFSG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIS KAEYDEAGPG IVHRKCF
Length:377
Mass (Da):41,863
Last modified:November 25, 2002 - v2
Checksum:iE9553D52F3C6D7B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3091S → L in AAC49523. (PubMed:8758981)Curated
Sequence conflicti320 – 3201T → R in CAA79065. (PubMed:8281187)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42007 Genomic DNA. Translation: AAC49523.1.
AC016041 Genomic DNA. Translation: AAF69724.1.
CP002684 Genomic DNA. Translation: AEE32408.1.
AF370302 mRNA. Translation: AAK44117.1.
AY063089 mRNA. Translation: AAL34263.1.
AY124004 mRNA. Translation: AAM74512.1.
Z17778 mRNA. Translation: CAA79065.1.
RefSeqiNP_175350.1. NM_103814.3.
UniGeneiAt.24689.

Genome annotation databases

EnsemblPlantsiAT1G49240.1; AT1G49240.1; AT1G49240.
GeneIDi841347.
KEGGiath:AT1G49240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42007 Genomic DNA. Translation: AAC49523.1 .
AC016041 Genomic DNA. Translation: AAF69724.1 .
CP002684 Genomic DNA. Translation: AEE32408.1 .
AF370302 mRNA. Translation: AAK44117.1 .
AY063089 mRNA. Translation: AAL34263.1 .
AY124004 mRNA. Translation: AAM74512.1 .
Z17778 mRNA. Translation: CAA79065.1 .
RefSeqi NP_175350.1. NM_103814.3.
UniGenei At.24689.

3D structure databases

ProteinModelPortali Q96293.
SMRi Q96293. Positions 10-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 26572. 5 interactions.
IntActi Q96293. 2 interactions.

2D gel databases

SWISS-2DPAGE P99055.

Proteomic databases

PaxDbi Q96293.
PRIDEi Q96293.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G49240.1 ; AT1G49240.1 ; AT1G49240 .
GeneIDi 841347.
KEGGi ath:AT1G49240.

Organism-specific databases

TAIRi AT1G49240.

Phylogenomic databases

eggNOGi COG5277.
HOGENOMi HOG000233340.
InParanoidi Q96293.
KOi K10355.
OMAi PPFYRIP.
PhylomeDBi Q96293.

Enzyme and pathway databases

Reactomei REACT_190901. Factors involved in megakaryocyte development and platelet production.
REACT_236222. Prefoldin mediated transfer of substrate to CCT/TriC.
REACT_247245. Folding of actin by CCT/TriC.

Gene expression databases

ExpressionAtlasi Q96293. baseline and differential.
Genevestigatori Q96293.

Family and domain databases

InterProi IPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view ]
PANTHERi PTHR11937. PTHR11937. 1 hit.
Pfami PF00022. Actin. 1 hit.
[Graphical view ]
PRINTSi PR00190. ACTIN.
SMARTi SM00268. ACTIN. 1 hit.
[Graphical view ]
PROSITEi PS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues."
    An Y.-Q., McDowell J.M., Huang S., McKinney E.C., Chambliss S., Meagher R.B.
    Plant J. 10:107-121(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 318-377.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  6. "Structure and evolution of the actin gene family in Arabidopsis thaliana."
    McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.
    Genetics 142:587-602(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, CHARACTERIZATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiACT8_ARATH
AccessioniPrimary (citable) accession number: Q96293
Secondary accession number(s): Q41933, Q9M9C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: November 26, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 8 actin genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3