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Q96293 (ACT8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-8
Gene names
Name:ACT8
Ordered Locus Names:At1g49240
ORF Names:F27J15.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins.

Enzyme regulation

Subject to negative translational control in pollen.

Subunit structure

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Subcellular location

Cytoplasmcytoskeleton.

Tissue specificity

Strongly expressed in nearly all vegetative tissues, and levels remain high in older tissues. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.

Miscellaneous

There are 8 actin genes in A.thaliana.

Sequence similarities

Belongs to the actin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Actin-8
PRO_0000088893

Experimental info

Sequence conflict3091S → L in AAC49523. Ref.1
Sequence conflict3201T → R in CAA79065. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q96293 [UniParc].

Last modified November 25, 2002. Version 2.
Checksum: E9553D52F3C6D7B4

FASTA37741,863
        10         20         30         40         50         60 
MADADDIQPI VCDNGTGMVK AGFAGDDAPR AVFPSVVGRP RHHGVMVGMN QKDAYVGDEA 

        70         80         90        100        110        120 
QSKRGILTLK YPIEHGVVSN WDDMEKIWHH TFYNELRIAP EEHPVLLTEA PLNPKANREK 

       130        140        150        160        170        180 
MTQIMFETFN SPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG FSLPHAILRL 

       190        200        210        220        230        240 
DLAGRDLTDY LMKILTERGY MFTTTAEREI VRDIKEKLSF VAVDYEQEME TSKTSSSIEK 

       250        260        270        280        290        300 
NYELPDGQVI TIGAERFRCP EVLFQPSFVG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV 

       310        320        330        340        350        360 
LSGGTTMFSG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIS 

       370 
KAEYDEAGPG IVHRKCF 

« Hide

References

« Hide 'large scale' references
[1]"Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues."
An Y.-Q., McDowell J.M., Huang S., McKinney E.C., Chambliss S., Meagher R.B.
Plant J. 10:107-121(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana."
Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. expand/collapse author list , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.
Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 318-377.
Strain: cv. Columbia.
Tissue: Green siliques.
[6]"Structure and evolution of the actin gene family in Arabidopsis thaliana."
McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.
Genetics 142:587-602(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, CHARACTERIZATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U42007 Genomic DNA. Translation: AAC49523.1.
AC016041 Genomic DNA. Translation: AAF69724.1.
CP002684 Genomic DNA. Translation: AEE32408.1.
AF370302 mRNA. Translation: AAK44117.1.
AY063089 mRNA. Translation: AAL34263.1.
AY124004 mRNA. Translation: AAM74512.1.
Z17778 mRNA. Translation: CAA79065.1.
RefSeqNP_175350.1. NM_103814.3.
UniGeneAt.24689.

3D structure databases

ProteinModelPortalQ96293.
SMRQ96293. Positions 10-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid26572. 5 interactions.
IntActQ96293. 2 interactions.

2D gel databases

SWISS-2DPAGEP99055.

Proteomic databases

PaxDbQ96293.
PRIDEQ96293.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G49240.1; AT1G49240.1; AT1G49240.
GeneID841347.
KEGGath:AT1G49240.

Organism-specific databases

TAIRAT1G49240.

Phylogenomic databases

eggNOGCOG5277.
HOGENOMHOG000233340.
InParanoidQ96293.
KOK10355.
OMAPPFYRIP.
PhylomeDBQ96293.

Gene expression databases

GenevestigatorQ96293.

Family and domain databases

InterProIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERPTHR11937. PTHR11937. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
PRINTSPR00190. ACTIN.
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACT8_ARATH
AccessionPrimary (citable) accession number: Q96293
Secondary accession number(s): Q41933, Q9M9C2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: May 14, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names