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Protein

Actin-8

Gene

ACT8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins.

Miscellaneous

There are 8 actin genes in A.thaliana.

Enzyme regulationi

Subject to negative translational control in pollen.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • mRNA binding Source: TAIR

GO - Biological processi

  • response to salt stress Source: TAIR
  • root hair cell tip growth Source: TAIR

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2029482 Regulation of actin dynamics for phagocytic cup formation
R-ATH-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-8
Gene namesi
Name:ACT8
Ordered Locus Names:At1g49240
ORF Names:F27J15.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G49240
TAIRilocus:2028416 AT1G49240

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000888932 – 377Actin-8Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ96293
PRIDEiQ96293

2D gel databases

SWISS-2DPAGEiP99055

PTM databases

iPTMnetiQ96293

Expressioni

Tissue specificityi

Strongly expressed in nearly all vegetative tissues, and levels remain high in older tissues. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.

Gene expression databases

ExpressionAtlasiQ96293 baseline and differential
GenevisibleiQ96293 AT

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Protein-protein interaction databases

BioGridi26572, 6 interactors
IntActiQ96293, 2 interactors
MINTiQ96293
STRINGi3702.AT1G49240.1

Structurei

3D structure databases

ProteinModelPortaliQ96293
SMRiQ96293
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676 Eukaryota
COG5277 LUCA
HOGENOMiHOG000233340
InParanoidiQ96293
KOiK10355
OMAiGEMETSK
OrthoDBiEOG09360BTG
PhylomeDBiQ96293

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR004001 Actin_CS
PANTHERiPTHR11937 PTHR11937, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
PRINTSiPR00190 ACTIN
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS00406 ACTINS_1, 1 hit
PS00432 ACTINS_2, 1 hit
PS01132 ACTINS_ACT_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADADDIQPI VCDNGTGMVK AGFAGDDAPR AVFPSVVGRP RHHGVMVGMN
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGVVSN WDDMEKIWHH TFYNELRIAP
110 120 130 140 150
EEHPVLLTEA PLNPKANREK MTQIMFETFN SPAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG FSLPHAILRL DLAGRDLTDY LMKILTERGY
210 220 230 240 250
MFTTTAEREI VRDIKEKLSF VAVDYEQEME TSKTSSSIEK NYELPDGQVI
260 270 280 290 300
TIGAERFRCP EVLFQPSFVG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGTTMFSG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIS KAEYDEAGPG IVHRKCF
Length:377
Mass (Da):41,863
Last modified:November 25, 2002 - v2
Checksum:iE9553D52F3C6D7B4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309S → L in AAC49523 (PubMed:8758981).Curated1
Sequence conflicti320T → R in CAA79065 (PubMed:8281187).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42007 Genomic DNA Translation: AAC49523.1
AC016041 Genomic DNA Translation: AAF69724.1
CP002684 Genomic DNA Translation: AEE32408.1
AF370302 mRNA Translation: AAK44117.1
AY063089 mRNA Translation: AAL34263.1
AY124004 mRNA Translation: AAM74512.1
Z17778 mRNA Translation: CAA79065.1
RefSeqiNP_175350.1, NM_103814.4
UniGeneiAt.24689

Genome annotation databases

EnsemblPlantsiAT1G49240.1; AT1G49240.1; AT1G49240
GeneIDi841347
GrameneiAT1G49240.1; AT1G49240.1; AT1G49240
KEGGiath:AT1G49240

Similar proteinsi

Entry informationi

Entry nameiACT8_ARATH
AccessioniPrimary (citable) accession number: Q96293
Secondary accession number(s): Q41933, Q9M9C2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: April 25, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health