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Protein

Actin-2

Gene

ACT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins.

Enzyme regulationi

Subject to negative translational control in pollen.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

GO - Biological processi

  • response to cytokinin Source: TAIR
  • response to far red light Source: TAIR
  • response to high light intensity Source: TAIR
  • response to red light Source: TAIR
  • root epidermal cell differentiation Source: TAIR
  • root hair cell tip growth Source: TAIR
  • root hair elongation Source: TAIR
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-2
Gene namesi
Name:ACT2
Ordered Locus Names:At3g18780
ORF Names:MVE11.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G18780.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytoskeleton Source: UniProtKB-SubCell
  • membrane Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 377376Actin-2PRO_0000088889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ96292.
PRIDEiQ96292.

Expressioni

Tissue specificityi

Strongly expressed in nearly all vegetative tissues, and remains high in older tissues. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.

Gene expression databases

ExpressionAtlasiQ96292. baseline and differential.
GenevisibleiQ96292. AT.

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Binary interactionsi

WithEntry#Exp.IntActNotes
HXK1Q425252EBI-1644538,EBI-1644489

Protein-protein interaction databases

BioGridi6744. 15 interactions.
IntActiQ96292. 9 interactions.
MINTiMINT-8069441.
STRINGi3702.AT3G18780.2.

Structurei

3D structure databases

ProteinModelPortaliQ96292.
SMRiQ96292. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
HOGENOMiHOG000233340.
InParanoidiQ96292.
KOiK10355.
OMAiCDEEISA.
PhylomeDBiQ96292.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q96292-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEADDIQPI VCDNGTGMVK AGFAGDDAPR AVFPSVVGRP RHHGVMVGMN
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGVVSN WDDMEKIWHH TFYNELRIAP
110 120 130 140 150
EEHPVLLTEA PLNPKANREK MTQIMFETFN SPAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG FSLPHAILRL DLAGRDLTDY LMKILTERGY
210 220 230 240 250
MFTTTAEREI VRDIKEKLSF VAVDYEQEME TSKTSSSIEK NYELPDGQVI
260 270 280 290 300
TIGAERFRCP EVLFQPSFVG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGTTMFSG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIS KAEYDEAGPG IVHRKCF
Length:377
Mass (Da):41,877
Last modified:February 1, 1997 - v1
Checksum:iF9E53D17F333D786
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41998 Genomic DNA. Translation: AAB37098.1.
AB026654 Genomic DNA. Translation: BAB01806.1.
CP002686 Genomic DNA. Translation: AEE76148.1.
AF428330 mRNA. Translation: AAL16260.1.
AY064043 mRNA. Translation: AAL36399.1.
AY096381 mRNA. Translation: AAM20022.1.
AY087751 mRNA. Translation: AAM65287.1.
Z25952 mRNA. Translation: CAA81112.1.
RefSeqiNP_188508.1. NM_112764.3. [Q96292-1]
UniGeneiAt.23604.
At.24689.
At.67767.

Genome annotation databases

EnsemblPlantsiAT3G18780.2; AT3G18780.2; AT3G18780. [Q96292-1]
GeneIDi821411.
KEGGiath:AT3G18780.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41998 Genomic DNA. Translation: AAB37098.1.
AB026654 Genomic DNA. Translation: BAB01806.1.
CP002686 Genomic DNA. Translation: AEE76148.1.
AF428330 mRNA. Translation: AAL16260.1.
AY064043 mRNA. Translation: AAL36399.1.
AY096381 mRNA. Translation: AAM20022.1.
AY087751 mRNA. Translation: AAM65287.1.
Z25952 mRNA. Translation: CAA81112.1.
RefSeqiNP_188508.1. NM_112764.3. [Q96292-1]
UniGeneiAt.23604.
At.24689.
At.67767.

3D structure databases

ProteinModelPortaliQ96292.
SMRiQ96292. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6744. 15 interactions.
IntActiQ96292. 9 interactions.
MINTiMINT-8069441.
STRINGi3702.AT3G18780.2.

Proteomic databases

PaxDbiQ96292.
PRIDEiQ96292.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G18780.2; AT3G18780.2; AT3G18780. [Q96292-1]
GeneIDi821411.
KEGGiath:AT3G18780.

Organism-specific databases

TAIRiAT3G18780.

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
HOGENOMiHOG000233340.
InParanoidiQ96292.
KOiK10355.
OMAiCDEEISA.
PhylomeDBiQ96292.

Miscellaneous databases

PROiQ96292.

Gene expression databases

ExpressionAtlasiQ96292. baseline and differential.
GenevisibleiQ96292. AT.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues."
    An Y.-Q., McDowell J.M., Huang S., McKinney E.C., Chambliss S., Meagher R.B.
    Plant J. 10:107-121(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-63.
    Strain: cv. Columbia.
    Tissue: Seedling.
  7. "Structure and evolution of the actin gene family in Arabidopsis thaliana."
    McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.
    Genetics 142:587-602(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, CHARACTERIZATION.
    Strain: cv. Columbia.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACT2_ARATH
AccessioniPrimary (citable) accession number: Q96292
Secondary accession number(s): Q42056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: February 1, 1997
Last modified: November 11, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 8 actin genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.