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Protein

2-Cys peroxiredoxin BAS1, chloroplastic

Gene

BAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf. Involved in the detoxification of alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system.1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

  • peroxidase activity Source: UniProtKB
  • peroxiredoxin activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • defense response to bacterium Source: TAIR
  • response to cold Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciARA:AT3G11630-MONOMER.
BRENDAi1.11.1.15. 399.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei4358. At2CysPrx01.

Names & Taxonomyi

Protein namesi
Recommended name:
2-Cys peroxiredoxin BAS1, chloroplastic (EC:1.11.1.15)
Short name:
2-Cys Prx A
Short name:
2-Cys peroxiredoxin A
Alternative name(s):
Thiol-specific antioxidant protein A
Gene namesi
Name:BAS1
Ordered Locus Names:At3g11630
ORF Names:F24K9.28, T19F11.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G11630.

Subcellular locationi

  • Plastidchloroplast 1 Publication

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • stromule Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65ChloroplastBy similarityAdd BLAST65
ChainiPRO_000002378466 – 2662-Cys peroxiredoxin BAS1, chloroplasticAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi119Interchain (with C-241); in linked formBy similarity
Disulfide bondi241Interchain (with C-119); in linked formBy similarity

Post-translational modificationi

The Cys-119-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-119 (probably Cys-SOH) rapidly reacts with Cys-241-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin (By similarity).By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ96291.
PRIDEiQ96291.

PTM databases

iPTMnetiQ96291.

Expressioni

Inductioni

Down-regulated under highly reduced cellular thiol pool conditions. Down-regulated by ascorbate. Slightly induced by oxidative stress.1 Publication

Gene expression databases

GenevisibleiQ96291. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation (By similarity). Interacts with the plastidial thioredoxin CDSP32. Interacts with the plastidial NADPH-dependent thioredoxin reductase ANTR-C.By similarity2 Publications

Protein-protein interaction databases

BioGridi5669. 10 interactors.
IntActiQ96291. 7 interactors.
STRINGi3702.AT3G11630.1.

Structurei

3D structure databases

ProteinModelPortaliQ96291.
SMRiQ96291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 232ThioredoxinPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Belongs to the AhpC/TSA family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
HOGENOMiHOG000022343.
InParanoidiQ96291.
KOiK03386.
OMAiVQPFKTE.
OrthoDBiEOG09360MKG.
PhylomeDBiQ96291.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVASSTTL ISSPSSRVFP AKSSLSSPSV SFLRTLSSPS ASASLRSGFA
60 70 80 90 100
RRSSLSSTSR RSFAVKAQAD DLPLVGNKAP DFEAEAVFDQ EFIKVKLSDY
110 120 130 140 150
IGKKYVILFF YPLDFTFVCP TEITAFSDRH SEFEKLNTEV LGVSVDSVFS
160 170 180 190 200
HLAWVQTDRK SGGLGDLNYP LISDVTKSIS KSFGVLIHDQ GIALRGLFII
210 220 230 240 250
DKEGVIQHST INNLGIGRSV DETMRTLQAL QYIQENPDEV CPAGWKPGEK
260
SMKPDPKLSK EYFSAI
Length:266
Mass (Da):29,092
Last modified:July 11, 2001 - v2
Checksum:i2CEB476A1A8694AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83E → K in CAA63909 (Ref. 1) Curated1
Sequence conflicti83E → K in CAA71503 (Ref. 1) Curated1
Sequence conflicti101I → N (Ref. 1) Curated1
Sequence conflicti101I → N (PubMed:9263459).Curated1
Sequence conflicti122Missing in CAA66484 (Ref. 1) Curated1
Sequence conflicti174 – 175DV → YF in AAM64537 (Ref. 7) Curated2
Sequence conflicti192I → IGI (PubMed:9263459).Curated1
Sequence conflicti218R → Q in AAM64537 (Ref. 7) Curated1
Sequence conflicti233 – 235IQE → TG in CAA63909 (Ref. 1) Curated3
Sequence conflicti233 – 235IQE → TG in CAA71503 (Ref. 1) Curated3
Sequence conflicti247P → S in CAA63909 (Ref. 1) Curated1
Sequence conflicti247P → S in CAA71503 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94218 mRNA. Translation: CAA63909.1.
Y10478 mRNA. Translation: CAA71503.1.
X97910 Genomic DNA. Translation: CAA66484.2.
AC008153 Genomic DNA. Translation: AAG51430.1.
AC009918 Genomic DNA. Translation: AAF02131.1.
CP002686 Genomic DNA. Translation: AEE75077.1.
AF324996 mRNA. Translation: AAG40348.1.
AF419578 mRNA. Translation: AAL31910.1.
AY079107 mRNA. Translation: AAL84991.1.
AY086974 mRNA. Translation: AAM64537.1.
RefSeqiNP_187769.1. NM_111995.3.
UniGeneiAt.22950.
At.68687.

Genome annotation databases

EnsemblPlantsiAT3G11630.1; AT3G11630.1; AT3G11630.
GeneIDi820335.
GrameneiAT3G11630.1; AT3G11630.1; AT3G11630.
KEGGiath:AT3G11630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94218 mRNA. Translation: CAA63909.1.
Y10478 mRNA. Translation: CAA71503.1.
X97910 Genomic DNA. Translation: CAA66484.2.
AC008153 Genomic DNA. Translation: AAG51430.1.
AC009918 Genomic DNA. Translation: AAF02131.1.
CP002686 Genomic DNA. Translation: AEE75077.1.
AF324996 mRNA. Translation: AAG40348.1.
AF419578 mRNA. Translation: AAL31910.1.
AY079107 mRNA. Translation: AAL84991.1.
AY086974 mRNA. Translation: AAM64537.1.
RefSeqiNP_187769.1. NM_111995.3.
UniGeneiAt.22950.
At.68687.

3D structure databases

ProteinModelPortaliQ96291.
SMRiQ96291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5669. 10 interactors.
IntActiQ96291. 7 interactors.
STRINGi3702.AT3G11630.1.

Protein family/group databases

PeroxiBasei4358. At2CysPrx01.

PTM databases

iPTMnetiQ96291.

Proteomic databases

PaxDbiQ96291.
PRIDEiQ96291.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G11630.1; AT3G11630.1; AT3G11630.
GeneIDi820335.
GrameneiAT3G11630.1; AT3G11630.1; AT3G11630.
KEGGiath:AT3G11630.

Organism-specific databases

TAIRiAT3G11630.

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
HOGENOMiHOG000022343.
InParanoidiQ96291.
KOiK03386.
OMAiVQPFKTE.
OrthoDBiEOG09360MKG.
PhylomeDBiQ96291.

Enzyme and pathway databases

BioCyciARA:AT3G11630-MONOMER.
BRENDAi1.11.1.15. 399.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ96291.

Gene expression databases

GenevisibleiQ96291. AT.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAS1A_ARATH
AccessioniPrimary (citable) accession number: Q96291
Secondary accession number(s): P92938, Q8L5U1, Q9S7Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2001
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.