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Protein

2-Cys peroxiredoxin BAS1, chloroplastic

Gene

BAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf.1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin CDSP32.1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

  • peroxidase activity Source: UniProtKB
  • peroxiredoxin activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • defense response to bacterium Source: TAIR
  • response to cold Source: TAIR

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciARA:AT3G11630-MONOMER.
BRENDAi1.11.1.15. 399.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei4358. At2CysPrx01.

Names & Taxonomyi

Protein namesi
Recommended name:
2-Cys peroxiredoxin BAS1, chloroplastic (EC:1.11.1.151 Publication)
Short name:
2-Cys Prx A
Short name:
2-Cys peroxiredoxin A
Alternative name(s):
Thiol-specific antioxidant protein A
Gene namesi
Name:BAS1
Ordered Locus Names:At3g11630
ORF Names:F24K9.28, T19F11.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G11630.
TAIRilocus:2080707. AT3G11630.

Subcellular locationi

  • Plastidchloroplast 1 Publication

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • stromule Source: TAIR
  • thylakoid Source: TAIR

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65ChloroplastBy similarityAdd BLAST65
ChainiPRO_000002378466 – 2662-Cys peroxiredoxin BAS1, chloroplasticAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi119Interchain (with C-241); in linked formBy similarity
Disulfide bondi241Interchain (with C-119); in linked formBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ96291.
PRIDEiQ96291.

PTM databases

iPTMnetiQ96291.

Expressioni

Inductioni

Down-regulated under highly reduced cellular thiol pool conditions. Down-regulated by ascorbate. Slightly induced by oxidative stress.1 Publication

Gene expression databases

ExpressionAtlasiQ96291. baseline and differential.
GenevisibleiQ96291. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation (By similarity). Interacts with the plastidial thioredoxin CDSP32 (PubMed:12084836). Interacts with the plastidial NADPH-dependent thioredoxin reductase ANTR-C (PubMed:16884685).By similarity2 Publications

Protein-protein interaction databases

BioGridi5669. 10 interactors.
IntActiQ96291. 7 interactors.
STRINGi3702.AT3G11630.1.

Structurei

3D structure databases

ProteinModelPortaliQ96291.
SMRiQ96291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 232ThioredoxinPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
HOGENOMiHOG000022343.
InParanoidiQ96291.
KOiK03386.
OMAiTAVHNGE.
OrthoDBiEOG09360MKG.
PhylomeDBiQ96291.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVASSTTL ISSPSSRVFP AKSSLSSPSV SFLRTLSSPS ASASLRSGFA
60 70 80 90 100
RRSSLSSTSR RSFAVKAQAD DLPLVGNKAP DFEAEAVFDQ EFIKVKLSDY
110 120 130 140 150
IGKKYVILFF YPLDFTFVCP TEITAFSDRH SEFEKLNTEV LGVSVDSVFS
160 170 180 190 200
HLAWVQTDRK SGGLGDLNYP LISDVTKSIS KSFGVLIHDQ GIALRGLFII
210 220 230 240 250
DKEGVIQHST INNLGIGRSV DETMRTLQAL QYIQENPDEV CPAGWKPGEK
260
SMKPDPKLSK EYFSAI
Length:266
Mass (Da):29,092
Last modified:July 11, 2001 - v2
Checksum:i2CEB476A1A8694AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83E → K in CAA63909 (Ref. 1) Curated1
Sequence conflicti83E → K in CAA71503 (Ref. 1) Curated1
Sequence conflicti101I → N (Ref. 1) Curated1
Sequence conflicti101I → N (PubMed:9263459).Curated1
Sequence conflicti122Missing in CAA66484 (Ref. 1) Curated1
Sequence conflicti174 – 175DV → YF in AAM64537 (Ref. 7) Curated2
Sequence conflicti192I → IGI (PubMed:9263459).Curated1
Sequence conflicti218R → Q in AAM64537 (Ref. 7) Curated1
Sequence conflicti233 – 235IQE → TG in CAA63909 (Ref. 1) Curated3
Sequence conflicti233 – 235IQE → TG in CAA71503 (Ref. 1) Curated3
Sequence conflicti247P → S in CAA63909 (Ref. 1) Curated1
Sequence conflicti247P → S in CAA71503 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94218 mRNA. Translation: CAA63909.1.
Y10478 mRNA. Translation: CAA71503.1.
X97910 Genomic DNA. Translation: CAA66484.2.
AC008153 Genomic DNA. Translation: AAG51430.1.
AC009918 Genomic DNA. Translation: AAF02131.1.
CP002686 Genomic DNA. Translation: AEE75077.1.
AF324996 mRNA. Translation: AAG40348.1.
AF419578 mRNA. Translation: AAL31910.1.
AY079107 mRNA. Translation: AAL84991.1.
AY086974 mRNA. Translation: AAM64537.1.
RefSeqiNP_187769.1. NM_111995.3.
UniGeneiAt.22950.
At.68687.

Genome annotation databases

EnsemblPlantsiAT3G11630.1; AT3G11630.1; AT3G11630.
GeneIDi820335.
GrameneiAT3G11630.1; AT3G11630.1; AT3G11630.
KEGGiath:AT3G11630.

Similar proteinsi

Entry informationi

Entry nameiBAS1A_ARATH
AccessioniPrimary (citable) accession number: Q96291
Secondary accession number(s): P92938, Q8L5U1, Q9S7Y0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2001
Last modified: August 30, 2017
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families