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Protein

Glutathione S-transferase F8, chloroplastic

Gene

GSTF8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • glutathione binding Source: TAIR
  • glutathione transferase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  • defense response Source: TAIR
  • defense response to bacterium Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • response to cold Source: TAIR
  • response to karrikin Source: TAIR
  • response to salt stress Source: TAIR
  • toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase, Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G47730-MONOMER.
MetaCyc:AT2G47730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F8, chloroplastic (EC:2.5.1.18)
Short name:
AtGSTF8
Alternative name(s):
AtGSTF5
GST class-phi member 8
Glutathione S-transferase 6
Gene namesi
Name:GSTF8
Synonyms:GST6, GSTF5
Ordered Locus Names:At2g47730
ORF Names:F17A22.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47730.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: TAIR
  • stromule Source: TAIR
  • thylakoid Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49ChloroplastSequence analysisAdd BLAST49
ChainiPRO_000018585050 – 263Glutathione S-transferase F8, chloroplasticAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96266.
PRIDEiQ96266.

PTM databases

iPTMnetiQ96266.

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in leaves and isoform 2 in roots.1 Publication

Inductioni

By salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, metolachlor, and the pathogen Hyaloperonospora parasitica.3 Publications

Gene expression databases

GenevisibleiQ96266. AT.

Interactioni

Protein-protein interaction databases

BioGridi4721. 1 interactor.
IntActiQ96266. 2 interactors.
STRINGi3702.AT2G47730.1.

Structurei

3D structure databases

ProteinModelPortaliQ96266.
SMRiQ96266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 131GST N-terminalAdd BLAST82
Domaini139 – 263GST C-terminalAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 61Glutathione bindingBy similarity2
Regioni89 – 90Glutathione bindingBy similarity2
Regioni102 – 103Glutathione bindingBy similarity2
Regioni115 – 116Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
InParanoidiQ96266.
KOiK00799.
OMAiAWAKVID.
OrthoDBiEOG09360M18.
PhylomeDBiQ96266.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96266-1) [UniParc]FASTAAdd to basket
Also known as: GSTF8-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA
60 70 80 90 100
SIKVHGVPMS TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF
110 120 130 140 150
GQIPALEDGD LTLFESRAIT QYLAEEYSEK GEKLISQDCK KVKATTNVWL
160 170 180 190 200
QVEGQQFDPN ASKLAFERVF KGMFGMTTDP AAVQELEGKL QKVLDVYEAR
210 220 230 240 250
LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK VSEWIKKISA
260
RPAWAKVIDL QKQ
Length:263
Mass (Da):29,232
Last modified:June 12, 2007 - v3
Checksum:i0A2927A7C4CA3047
GO
Isoform 2 (identifier: Q96266-2) [UniParc]FASTAAdd to basket
Also known as: GSTF8-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:215
Mass (Da):24,077
Checksum:iD7CBB8B1F6B74656
GO

Sequence cautioni

The sequence CAA64613 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0419351 – 48Missing in isoform 2. CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems.
AF288176 mRNA. Translation: AAG30125.2.
AC005309 Genomic DNA. Translation: AAC63629.2.
CP002685 Genomic DNA. Translation: AEC10880.1.
DQ069797 mRNA. Translation: AAY82256.1.
PIRiH84918.
RefSeqiNP_850479.1. NM_180148.5. [Q96266-1]
UniGeneiAt.25017.

Genome annotation databases

EnsemblPlantsiAT2G47730.1; AT2G47730.1; AT2G47730. [Q96266-1]
GeneIDi819386.
GrameneiAT2G47730.1; AT2G47730.1; AT2G47730.
KEGGiath:AT2G47730.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems.
AF288176 mRNA. Translation: AAG30125.2.
AC005309 Genomic DNA. Translation: AAC63629.2.
CP002685 Genomic DNA. Translation: AEC10880.1.
DQ069797 mRNA. Translation: AAY82256.1.
PIRiH84918.
RefSeqiNP_850479.1. NM_180148.5. [Q96266-1]
UniGeneiAt.25017.

3D structure databases

ProteinModelPortaliQ96266.
SMRiQ96266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4721. 1 interactor.
IntActiQ96266. 2 interactors.
STRINGi3702.AT2G47730.1.

PTM databases

iPTMnetiQ96266.

Proteomic databases

PaxDbiQ96266.
PRIDEiQ96266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47730.1; AT2G47730.1; AT2G47730. [Q96266-1]
GeneIDi819386.
GrameneiAT2G47730.1; AT2G47730.1; AT2G47730.
KEGGiath:AT2G47730.

Organism-specific databases

TAIRiAT2G47730.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
InParanoidiQ96266.
KOiK00799.
OMAiAWAKVID.
OrthoDBiEOG09360M18.
PhylomeDBiQ96266.

Enzyme and pathway databases

BioCyciARA:AT2G47730-MONOMER.
MetaCyc:AT2G47730-MONOMER.

Miscellaneous databases

PROiQ96266.

Gene expression databases

GenevisibleiQ96266. AT.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTF8_ARATH
AccessioniPrimary (citable) accession number: Q96266
Secondary accession number(s): O82242, Q4PL91, Q9FUT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 12, 2007
Last modified: November 30, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.