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Protein

Glutathione S-transferase F8, chloroplastic

Gene

GSTF8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • glutathione binding Source: TAIR
  • glutathione transferase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  • defense response Source: TAIR
  • defense response to bacterium Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • response to cold Source: TAIR
  • response to karrikin Source: TAIR
  • response to salt stress Source: TAIR
  • toxin catabolic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase, Peroxidase, Transferase
Biological processDetoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G47730-MONOMER
MetaCyc:AT2G47730-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F8, chloroplastic (EC:2.5.1.18)
Short name:
AtGSTF8
Alternative name(s):
AtGSTF5
GST class-phi member 8
Glutathione S-transferase 6
Gene namesi
Name:GSTF8
Synonyms:GST6, GSTF5
Ordered Locus Names:At2g47730
ORF Names:F17A22.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47730
TAIRilocus:2043298 AT2G47730

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49ChloroplastSequence analysisAdd BLAST49
ChainiPRO_000018585050 – 263Glutathione S-transferase F8, chloroplasticAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96266
PRIDEiQ96266

PTM databases

iPTMnetiQ96266

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in leaves and isoform 2 in roots.1 Publication

Inductioni

By salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, metolachlor, and the pathogen Hyaloperonospora parasitica.3 Publications

Gene expression databases

ExpressionAtlasiQ96266 baseline and differential
GenevisibleiQ96266 AT

Interactioni

Protein-protein interaction databases

BioGridi4721, 1 interactor
IntActiQ96266, 2 interactors
STRINGi3702.AT2G47730.1

Structurei

3D structure databases

ProteinModelPortaliQ96266
SMRiQ96266
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 131GST N-terminalAdd BLAST82
Domaini139 – 263GST C-terminalAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 61Glutathione bindingBy similarity2
Regioni89 – 90Glutathione bindingBy similarity2
Regioni102 – 103Glutathione bindingBy similarity2
Regioni115 – 116Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0867 Eukaryota
COG0625 LUCA
HOGENOMiHOG000125746
InParanoidiQ96266
KOiK00799
OMAiPAIHYLM
OrthoDBiEOG09360M18
PhylomeDBiQ96266

Family and domain databases

CDDicd03187 GST_C_Phi, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR034347 GST_Phi_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96266-1) [UniParc]FASTAAdd to basket
Also known as: GSTF8-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA
60 70 80 90 100
SIKVHGVPMS TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF
110 120 130 140 150
GQIPALEDGD LTLFESRAIT QYLAEEYSEK GEKLISQDCK KVKATTNVWL
160 170 180 190 200
QVEGQQFDPN ASKLAFERVF KGMFGMTTDP AAVQELEGKL QKVLDVYEAR
210 220 230 240 250
LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK VSEWIKKISA
260
RPAWAKVIDL QKQ
Length:263
Mass (Da):29,232
Last modified:June 12, 2007 - v3
Checksum:i0A2927A7C4CA3047
GO
Isoform 2 (identifier: Q96266-2) [UniParc]FASTAAdd to basket
Also known as: GSTF8-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:215
Mass (Da):24,077
Checksum:iD7CBB8B1F6B74656
GO

Sequence cautioni

The sequence CAA64613 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0419351 – 48Missing in isoform 2. CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95295 Genomic DNA Translation: CAA64613.1 Sequence problems.
AF288176 mRNA Translation: AAG30125.2
AC005309 Genomic DNA Translation: AAC63629.2
CP002685 Genomic DNA Translation: AEC10880.1
CP002685 Genomic DNA Translation: ANM61251.1
DQ069797 mRNA Translation: AAY82256.1
PIRiH84918
RefSeqiNP_001323480.1, NM_001337273.1 [Q96266-1]
NP_850479.1, NM_180148.5 [Q96266-1]
UniGeneiAt.25017

Genome annotation databases

EnsemblPlantsiAT2G47730.1; AT2G47730.1; AT2G47730 [Q96266-1]
AT2G47730.2; AT2G47730.2; AT2G47730 [Q96266-1]
GeneIDi819386
GrameneiAT2G47730.1; AT2G47730.1; AT2G47730 [Q96266-1]
AT2G47730.2; AT2G47730.2; AT2G47730 [Q96266-1]
KEGGiath:AT2G47730

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGSTF8_ARATH
AccessioniPrimary (citable) accession number: Q96266
Secondary accession number(s): O82242, Q4PL91, Q9FUT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 12, 2007
Last modified: April 25, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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