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Q96266

- GSTF8_ARATH

UniProt

Q96266 - GSTF8_ARATH

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Protein

Glutathione S-transferase F8, chloroplastic

Gene
GSTF8, GST6, GSTF5, At2g47730, F17A22.12
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. glutathione binding Source: TAIR
  2. glutathione transferase activity Source: TAIR
  3. peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  1. defense response Source: TAIR
  2. defense response to bacterium Source: TAIR
  3. response to cold Source: TAIR
  4. response to karrikin Source: TAIR
  5. response to salt stress Source: TAIR
  6. response to toxic substance Source: UniProtKB-KW
  7. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase, Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G47730-MONOMER.
MetaCyc:AT2G47730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F8, chloroplastic (EC:2.5.1.18)
Short name:
AtGSTF8
Alternative name(s):
AtGSTF5
GST class-phi member 8
Glutathione S-transferase 6
Gene namesi
Name:GSTF8
Synonyms:GST6, GSTF5
Ordered Locus Names:At2g47730
ORF Names:F17A22.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G47730.

Subcellular locationi

Plastidchloroplast. Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytosol Source: UniProtKB-SubCell
  5. nucleus Source: TAIR
  6. stromule Source: TAIR
  7. thylakoid Source: TAIR
  8. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949Chloroplast Reviewed predictionAdd
BLAST
Chaini50 – 263214Glutathione S-transferase F8, chloroplasticPRO_0000185850Add
BLAST

Proteomic databases

PaxDbiQ96266.
PRIDEiQ96266.

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in leaves and isoform 2 in roots.1 Publication

Inductioni

By salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, metolachlor, and the pathogen Hyaloperonospora parasitica.3 Publications

Gene expression databases

ArrayExpressiQ96266.
GenevestigatoriQ96266.

Interactioni

Protein-protein interaction databases

BioGridi4721. 1 interaction.
IntActiQ96266. 2 interactions.
STRINGi3702.AT2G47730.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ96266.
SMRiQ96266. Positions 22-263.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 13182GST N-terminalAdd
BLAST
Domaini139 – 263125GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 612Glutathione binding By similarity
Regioni89 – 902Glutathione binding By similarity
Regioni102 – 1032Glutathione binding By similarity
Regioni115 – 1162Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125746.
InParanoidiQ96266.
KOiK00799.
OMAiAWAKVID.
PhylomeDBiQ96266.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96266-1) [UniParc]FASTAAdd to Basket

Also known as: GSTF8-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA    50
SIKVHGVPMS TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF 100
GQIPALEDGD LTLFESRAIT QYLAEEYSEK GEKLISQDCK KVKATTNVWL 150
QVEGQQFDPN ASKLAFERVF KGMFGMTTDP AAVQELEGKL QKVLDVYEAR 200
LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK VSEWIKKISA 250
RPAWAKVIDL QKQ 263
Length:263
Mass (Da):29,232
Last modified:June 12, 2007 - v3
Checksum:i0A2927A7C4CA3047
GO
Isoform 2 (identifier: Q96266-2) [UniParc]FASTAAdd to Basket

Also known as: GSTF8-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:215
Mass (Da):24,077
Checksum:iD7CBB8B1F6B74656
GO

Sequence cautioni

The sequence CAA64613.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4848Missing in isoform 2. VSP_041935Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems.
AF288176 mRNA. Translation: AAG30125.2.
AC005309 Genomic DNA. Translation: AAC63629.2.
CP002685 Genomic DNA. Translation: AEC10880.1.
DQ069797 mRNA. Translation: AAY82256.1.
PIRiH84918.
RefSeqiNP_850479.1. NM_180148.4. [Q96266-1]
UniGeneiAt.25017.

Genome annotation databases

EnsemblPlantsiAT2G47730.1; AT2G47730.1; AT2G47730. [Q96266-1]
GeneIDi819386.
KEGGiath:AT2G47730.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95295 Genomic DNA. Translation: CAA64613.1 . Sequence problems.
AF288176 mRNA. Translation: AAG30125.2 .
AC005309 Genomic DNA. Translation: AAC63629.2 .
CP002685 Genomic DNA. Translation: AEC10880.1 .
DQ069797 mRNA. Translation: AAY82256.1 .
PIRi H84918.
RefSeqi NP_850479.1. NM_180148.4. [Q96266-1 ]
UniGenei At.25017.

3D structure databases

ProteinModelPortali Q96266.
SMRi Q96266. Positions 22-263.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4721. 1 interaction.
IntActi Q96266. 2 interactions.
STRINGi 3702.AT2G47730.1-P.

Proteomic databases

PaxDbi Q96266.
PRIDEi Q96266.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G47730.1 ; AT2G47730.1 ; AT2G47730 . [Q96266-1 ]
GeneIDi 819386.
KEGGi ath:AT2G47730.

Organism-specific databases

TAIRi AT2G47730.

Phylogenomic databases

eggNOGi COG0625.
HOGENOMi HOG000125746.
InParanoidi Q96266.
KOi K00799.
OMAi AWAKVID.
PhylomeDBi Q96266.

Enzyme and pathway databases

BioCyci ARA:AT2G47730-MONOMER.
MetaCyc:AT2G47730-MONOMER.

Gene expression databases

ArrayExpressi Q96266.
Genevestigatori Q96266.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites."
    Chen W., Chao G., Singh K.B.
    Plant J. 10:955-966(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: cv. Columbia.
  2. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants."
    Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D.
    Plant Physiol. 139:1323-1337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner."
    Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B.
    Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Differential gene expression and subcellular targeting of Arabidopsis glutathione S-transferase F8 is achieved through alternative transcription start sites."
    Thatcher L.F., Carrie C., Andersson C.R., Sivasithamparam K., Whelan J., Singh K.B.
    J. Biol. Chem. 282:28915-28928(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry."
    Aryal U.K., Krochko J.E., Ross A.R.
    J. Proteome Res. 11:425-437(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGSTF8_ARATH
AccessioniPrimary (citable) accession number: Q96266
Secondary accession number(s): O82242, Q4PL91, Q9FUT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 12, 2007
Last modified: June 11, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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