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Q96266 (GSTF8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase F8, chloroplastic

Short name=AtGSTF8
EC=2.5.1.18
Alternative name(s):
AtGSTF5
GST class-phi member 8
Glutathione S-transferase 6
Gene names
Name:GSTF8
Synonyms:GST6, GSTF5
Ordered Locus Names:At2g47730
ORF Names:F17A22.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.2

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subcellular location

Plastidchloroplast. Cytoplasmcytosol Ref.7.

Tissue specificity

Isoform 1 is predominantly expressed in leaves and isoform 2 in roots. Ref.7

Induction

By salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, metolachlor, and the pathogen Hyaloperonospora parasitica. Ref.1 Ref.2 Ref.6

Sequence similarities

Belongs to the GST superfamily. Phi family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Sequence caution

The sequence CAA64613.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processDetoxification
Stress response
   Cellular componentChloroplast
Cytoplasm
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   Molecular functionOxidoreductase
Peroxidase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response

Inferred from expression pattern PubMed 10571852. Source: TAIR

defense response to bacterium

Inferred from expression pattern PubMed 17028151. Source: TAIR

response to cold

Inferred from expression pattern PubMed 16923014. Source: TAIR

response to karrikin

Inferred from expression pattern PubMed 20351290. Source: TAIR

response to salt stress

Inferred from expression pattern PubMed 17916636. Source: TAIR

response to toxic substance

Inferred from electronic annotation. Source: UniProtKB-KW

toxin catabolic process

Traceable author statement Ref.2. Source: TAIR

   Cellular_componentchloroplast

Inferred from direct assay PubMed 18431481. Source: TAIR

chloroplast envelope

Inferred from direct assay PubMed 12938931. Source: TAIR

chloroplast stroma

Inferred from direct assay PubMed 18633119PubMed 20061580. Source: TAIR

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 14617066. Source: TAIR

stromule

Inferred from direct assay PubMed 16923014. Source: TAIR

thylakoid

Inferred from direct assay PubMed 16648217. Source: TAIR

vacuolar membrane

Inferred from direct assay PubMed 17151019. Source: TAIR

   Molecular_functionglutathione binding

Inferred from direct assay PubMed 15159623. Source: TAIR

glutathione transferase activity

Inferred from direct assay PubMed 18334669. Source: TAIR

peroxidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96266-1)

Also known as: GSTF8-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96266-2)

Also known as: GSTF8-S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Potential
Chain50 – 263214Glutathione S-transferase F8, chloroplastic
PRO_0000185850

Regions

Domain50 – 13182GST N-terminal
Domain139 – 263125GST C-terminal
Region60 – 612Glutathione binding By similarity
Region89 – 902Glutathione binding By similarity
Region102 – 1032Glutathione binding By similarity
Region115 – 1162Glutathione binding By similarity

Natural variations

Alternative sequence1 – 4848Missing in isoform 2.
VSP_041935

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (GSTF8-L) [UniParc].

Last modified June 12, 2007. Version 3.
Checksum: 0A2927A7C4CA3047

FASTA26329,232
        10         20         30         40         50         60 
MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA SIKVHGVPMS 

        70         80         90        100        110        120 
TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF GQIPALEDGD LTLFESRAIT 

       130        140        150        160        170        180 
QYLAEEYSEK GEKLISQDCK KVKATTNVWL QVEGQQFDPN ASKLAFERVF KGMFGMTTDP 

       190        200        210        220        230        240 
AAVQELEGKL QKVLDVYEAR LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK 

       250        260 
VSEWIKKISA RPAWAKVIDL QKQ 

« Hide

Isoform 2 (GSTF8-S) [UniParc].

Checksum: D7CBB8B1F6B74656
Show »

FASTA21524,077

References

« Hide 'large scale' references
[1]"The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites."
Chen W., Chao G., Singh K.B.
Plant J. 10:955-966(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: cv. Columbia.
[2]"Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
Wagner U., Edwards R., Dixon D.P., Mauch F.
Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants."
Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D.
Plant Physiol. 139:1323-1337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner."
Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B.
Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Differential gene expression and subcellular targeting of Arabidopsis glutathione S-transferase F8 is achieved through alternative transcription start sites."
Thatcher L.F., Carrie C., Andersson C.R., Sivasithamparam K., Whelan J., Singh K.B.
J. Biol. Chem. 282:28915-28928(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry."
Aryal U.K., Krochko J.E., Ross A.R.
J. Proteome Res. 11:425-437(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems.
AF288176 mRNA. Translation: AAG30125.2.
AC005309 Genomic DNA. Translation: AAC63629.2.
CP002685 Genomic DNA. Translation: AEC10880.1.
DQ069797 mRNA. Translation: AAY82256.1.
PIRH84918.
RefSeqNP_850479.1. NM_180148.4.
UniGeneAt.25017.

3D structure databases

ProteinModelPortalQ96266.
SMRQ96266. Positions 52-257.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4721. 1 interaction.
IntActQ96266. 2 interactions.
STRING3702.AT2G47730.1-P.

Proteomic databases

PaxDbQ96266.
PRIDEQ96266.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G47730.1; AT2G47730.1; AT2G47730. [Q96266-1]
GeneID819386.
KEGGath:AT2G47730.

Organism-specific databases

TAIRAT2G47730.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125746.
InParanoidQ96266.
KOK00799.
OMAAWAKVID.
PhylomeDBQ96266.
ProtClustDBCLSN2918292.

Enzyme and pathway databases

BioCycARA:AT2G47730-MONOMER.
MetaCyc:AT2G47730-MONOMER.

Gene expression databases

ArrayExpressQ96266.
GenevestigatorQ96266.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTF8_ARATH
AccessionPrimary (citable) accession number: Q96266
Secondary accession number(s): O82242, Q4PL91, Q9FUT2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names