Reviewed, UniProtKB/Swiss-Prot Q96266 (GSTF6_ARATH)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase 6, chloroplastic
    EC=2.5.1.18
Alternative name(s):
    GST class phi

Gene names

Name:	GST6
Ordered Locus Names:	At2g47730
ORF Names:	F17A22.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. In plants, may have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence CAA64613.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from expression pattern. Source: TAIR response to cold Inferred from expression pattern. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR toxin catabolic process Traceable author statement. Source: TAIR
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Molecular function	glutathione binding Inferred from direct assay. Source: TAIR glutathione transferase activity Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449374,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 263	214	Glutathione S-transferase 6, chloroplastic		PRO_0000185850
Regions
	Domain	50 – 131	82	GST N-terminal
	Domain	139 – 263	125	GST C-terminal

Sequences

	Sequence		Length	Mass (Da)
	Q96266-1 [UniParc]. Last modified June 12, 2007. Version 3. Checksum: 0A2927A7C4CA3047	FASTA	263	29,232
	10 20 30 40 50 60 MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA SIKVHGVPMS 70 80 90 100 110 120 TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF GQIPALEDGD LTLFESRAIT 130 140 150 160 170 180 QYLAEEYSEK GEKLISQDCK KVKATTNVWL QVEGQQFDPN ASKLAFERVF KGMFGMTTDP 190 200 210 220 230 240 AAVQELEGKL QKVLDVYEAR LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK 250 260 VSEWIKKISA RPAWAKVIDL QKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites." Chen W., Chao G., Singh K.B. Plant J. 10:955-966(1996) [PubMed: 9011080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Analysis of the glutathione S-transferase family in Arabidopsis thaliana." Wagner U., Mauch F. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants." Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D. Plant Physiol. 139:1323-1337(2005) [PubMed: 16244158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems. AF288176 mRNA. Translation: AAG30125.2. AC005309 Genomic DNA. Translation: AAC63629.2. Different initiation. DQ069797 mRNA. Translation: AAY82256.1.
IPI	IPI00536062.
PIR	H84918.
RefSeq	NP_850479.1.
UniGene	At.25017 At.72604
3D structure databases
HSSP	HSSP built from PDB template 1GNW based on UniProtKB P46422.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96266. 1 interaction.
Proteomic databases
PRIDE	Q96266.
Genome annotation databases
GeneID	819386.
GenomeReviews	Gene locus AT2G47730 in contig CT485783_GR.
KEGG	ath:AT2G47730.
NMPDR	fig|3702.1.peg.11960.
Organism-specific databases
TAIR	At2g47730.
Phylogenomic databases
OMA	Q96266. SIIMASI.
Enzyme and pathway databases
BRENDA	2.5.1.18. 302.
Gene expression databases
ArrayExpress	Q96266.
GermOnline	AT2G47730. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF6_ARATH

Accession

Primary (citable) accession number: Q96266
Secondary accession number(s): O82242, Q4PL91, Q9FUT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 12, 2007
Last modified:	June 16, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)