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Q96266

- GSTF8_ARATH

UniProt

Q96266 - GSTF8_ARATH

Protein

Glutathione S-transferase F8, chloroplastic

Gene

GSTF8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    GO - Molecular functioni

    1. glutathione binding Source: TAIR
    2. glutathione transferase activity Source: TAIR
    3. peroxidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. defense response Source: TAIR
    2. defense response to bacterium Source: TAIR
    3. response to cold Source: TAIR
    4. response to karrikin Source: TAIR
    5. response to salt stress Source: TAIR
    6. response to toxic substance Source: UniProtKB-KW
    7. toxin catabolic process Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase, Transferase

    Keywords - Biological processi

    Detoxification, Stress response

    Enzyme and pathway databases

    BioCyciARA:AT2G47730-MONOMER.
    MetaCyc:AT2G47730-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase F8, chloroplastic (EC:2.5.1.18)
    Short name:
    AtGSTF8
    Alternative name(s):
    AtGSTF5
    GST class-phi member 8
    Glutathione S-transferase 6
    Gene namesi
    Name:GSTF8
    Synonyms:GST6, GSTF5
    Ordered Locus Names:At2g47730
    ORF Names:F17A22.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G47730.

    Subcellular locationi

    Plastidchloroplast 1 Publication. Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. chloroplast stroma Source: TAIR
    4. cytosol Source: UniProtKB-SubCell
    5. nucleus Source: TAIR
    6. stromule Source: TAIR
    7. thylakoid Source: TAIR
    8. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Cytoplasm, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
    BLAST
    Chaini50 – 263214Glutathione S-transferase F8, chloroplasticPRO_0000185850Add
    BLAST

    Proteomic databases

    PaxDbiQ96266.
    PRIDEiQ96266.

    Expressioni

    Tissue specificityi

    Isoform 1 is predominantly expressed in leaves and isoform 2 in roots.1 Publication

    Inductioni

    By salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, metolachlor, and the pathogen Hyaloperonospora parasitica.3 Publications

    Gene expression databases

    ArrayExpressiQ96266.
    GenevestigatoriQ96266.

    Interactioni

    Protein-protein interaction databases

    BioGridi4721. 1 interaction.
    IntActiQ96266. 2 interactions.
    STRINGi3702.AT2G47730.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96266.
    SMRiQ96266. Positions 22-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini50 – 13182GST N-terminalAdd
    BLAST
    Domaini139 – 263125GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni60 – 612Glutathione bindingBy similarity
    Regioni89 – 902Glutathione bindingBy similarity
    Regioni102 – 1032Glutathione bindingBy similarity
    Regioni115 – 1162Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Phi family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125746.
    InParanoidiQ96266.
    KOiK00799.
    OMAiAWAKVID.
    PhylomeDBiQ96266.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96266-1) [UniParc]FASTAAdd to Basket

    Also known as: GSTF8-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA    50
    SIKVHGVPMS TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF 100
    GQIPALEDGD LTLFESRAIT QYLAEEYSEK GEKLISQDCK KVKATTNVWL 150
    QVEGQQFDPN ASKLAFERVF KGMFGMTTDP AAVQELEGKL QKVLDVYEAR 200
    LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK VSEWIKKISA 250
    RPAWAKVIDL QKQ 263
    Length:263
    Mass (Da):29,232
    Last modified:June 12, 2007 - v3
    Checksum:i0A2927A7C4CA3047
    GO
    Isoform 2 (identifier: Q96266-2) [UniParc]FASTAAdd to Basket

    Also known as: GSTF8-S

    The sequence of this isoform differs from the canonical sequence as follows:
         1-48: Missing.

    Show »
    Length:215
    Mass (Da):24,077
    Checksum:iD7CBB8B1F6B74656
    GO

    Sequence cautioni

    The sequence CAA64613.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4848Missing in isoform 2. CuratedVSP_041935Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems.
    AF288176 mRNA. Translation: AAG30125.2.
    AC005309 Genomic DNA. Translation: AAC63629.2.
    CP002685 Genomic DNA. Translation: AEC10880.1.
    DQ069797 mRNA. Translation: AAY82256.1.
    PIRiH84918.
    RefSeqiNP_850479.1. NM_180148.4. [Q96266-1]
    UniGeneiAt.25017.

    Genome annotation databases

    EnsemblPlantsiAT2G47730.1; AT2G47730.1; AT2G47730. [Q96266-1]
    GeneIDi819386.
    KEGGiath:AT2G47730.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95295 Genomic DNA. Translation: CAA64613.1 . Sequence problems.
    AF288176 mRNA. Translation: AAG30125.2 .
    AC005309 Genomic DNA. Translation: AAC63629.2 .
    CP002685 Genomic DNA. Translation: AEC10880.1 .
    DQ069797 mRNA. Translation: AAY82256.1 .
    PIRi H84918.
    RefSeqi NP_850479.1. NM_180148.4. [Q96266-1 ]
    UniGenei At.25017.

    3D structure databases

    ProteinModelPortali Q96266.
    SMRi Q96266. Positions 22-263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4721. 1 interaction.
    IntActi Q96266. 2 interactions.
    STRINGi 3702.AT2G47730.1-P.

    Proteomic databases

    PaxDbi Q96266.
    PRIDEi Q96266.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G47730.1 ; AT2G47730.1 ; AT2G47730 . [Q96266-1 ]
    GeneIDi 819386.
    KEGGi ath:AT2G47730.

    Organism-specific databases

    TAIRi AT2G47730.

    Phylogenomic databases

    eggNOGi COG0625.
    HOGENOMi HOG000125746.
    InParanoidi Q96266.
    KOi K00799.
    OMAi AWAKVID.
    PhylomeDBi Q96266.

    Enzyme and pathway databases

    BioCyci ARA:AT2G47730-MONOMER.
    MetaCyc:AT2G47730-MONOMER.

    Gene expression databases

    ArrayExpressi Q96266.
    Genevestigatori Q96266.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites."
      Chen W., Chao G., Singh K.B.
      Plant J. 10:955-966(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: cv. Columbia.
    2. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
      Wagner U., Edwards R., Dixon D.P., Mauch F.
      Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants."
      Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D.
      Plant Physiol. 139:1323-1337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner."
      Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B.
      Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Differential gene expression and subcellular targeting of Arabidopsis glutathione S-transferase F8 is achieved through alternative transcription start sites."
      Thatcher L.F., Carrie C., Andersson C.R., Sivasithamparam K., Whelan J., Singh K.B.
      J. Biol. Chem. 282:28915-28928(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry."
      Aryal U.K., Krochko J.E., Ross A.R.
      J. Proteome Res. 11:425-437(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGSTF8_ARATH
    AccessioniPrimary (citable) accession number: Q96266
    Secondary accession number(s): O82242, Q4PL91, Q9FUT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3