Q96266 (GSTF8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase F8, chloroplastic
Short name=AtGSTF8
EC=2.5.1.18

Alternative name(s):
AtGSTF5
GST class-phi member 8
Glutathione S-transferase 6

Gene names

Name:	GSTF8
Synonyms:	GST6, GSTF5
Ordered Locus Names:	At2g47730
ORF Names:	F17A22.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.2
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Plastid › chloroplast. Cytoplasm › cytosol Ref.7.
Tissue specificity	Isoform 1 is predominantly expressed in leaves and isoform 2 in roots. Ref.7
Induction	By salicylic acid, ethylene, methyl jasmonate, auxin, H2O2, metolachlor, and the pathogen Hyaloperonospora parasitica. Ref.1 Ref.2 Ref.6
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence CAA64613.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Detoxification Stress response
Cellular component	Chloroplast Cytoplasm Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Oxidoreductase Peroxidase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from expression pattern PubMed 17028151. Source: TAIR response to cold Inferred from expression pattern PubMed 16923014. Source: TAIR response to karrikin Inferred from expression pattern PubMed 20351290. Source: TAIR response to salt stress Inferred from expression pattern PubMed 17916636. Source: TAIR response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.2. Source: TAIR
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12938931. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 18633119PubMed 20061580. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 14617066. Source: TAIR stromule Inferred from direct assay PubMed 16923014. Source: TAIR thylakoid Inferred from direct assay PubMed 16648217. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	glutathione binding Inferred from direct assay PubMed 15159623. Source: TAIR glutathione transferase activity Inferred from direct assay PubMed 18334669. Source: TAIR peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96266-1)

Also known as: GSTF8-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96266-2)

Also known as: GSTF8-S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 263	214	Glutathione S-transferase F8, chloroplastic		PRO_0000185850
Regions
	Domain	50 – 131	82	GST N-terminal
	Domain	139 – 263	125	GST C-terminal
	Region	60 – 61	2	Glutathione binding By similarity
	Region	89 – 90	2	Glutathione binding By similarity
	Region	102 – 103	2	Glutathione binding By similarity
	Region	115 – 116	2	Glutathione binding By similarity
Natural variations
	Alternative sequence	1 – 48	48	Missing in isoform 2.		VSP_041935

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (GSTF8-L) [UniParc]. Last modified June 12, 2007. Version 3. Checksum: 0A2927A7C4CA3047	FASTA	263	29,232
	10 20 30 40 50 60 MGAIQARLPL FLSPPSIKHH TFLHSSSSNS NFKIRSNKSS SSSSSSIIMA SIKVHGVPMS 70 80 90 100 110 120 TATMRVLATL YEKDLQFELI PVDMRAGAHK QEAHLALNPF GQIPALEDGD LTLFESRAIT 130 140 150 160 170 180 QYLAEEYSEK GEKLISQDCK KVKATTNVWL QVEGQQFDPN ASKLAFERVF KGMFGMTTDP 190 200 210 220 230 240 AAVQELEGKL QKVLDVYEAR LAKSEFLAGD SFTLADLHHL PAIHYLLGTD SKVLFDSRPK 250 260 VSEWIKKISA RPAWAKVIDL QKQ
	Isoform 2 (GSTF8-S) [UniParc]. Checksum: D7CBB8B1F6B74656	FASTA	215	24,077
	10 20 30 40 50 60 MASIKVHGVP MSTATMRVLA TLYEKDLQFE LIPVDMRAGA HKQEAHLALN PFGQIPALED 70 80 90 100 110 120 GDLTLFESRA ITQYLAEEYS EKGEKLISQD CKKVKATTNV WLQVEGQQFD PNASKLAFER 130 140 150 160 170 180 VFKGMFGMTT DPAAVQELEG KLQKVLDVYE ARLAKSEFLA GDSFTLADLH HLPAIHYLLG 190 200 210 TDSKVLFDSR PKVSEWIKKI SARPAWAKVI DLQKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites." Chen W., Chao G., Singh K.B. Plant J. 10:955-966(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: cv. Columbia.
[2]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants." Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D. Plant Physiol. 139:1323-1337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner." Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B. Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Differential gene expression and subcellular targeting of Arabidopsis glutathione S-transferase F8 is achieved through alternative transcription start sites." Thatcher L.F., Carrie C., Andersson C.R., Sivasithamparam K., Whelan J., Singh K.B. J. Biol. Chem. 282:28915-28928(2007) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95295 Genomic DNA. Translation: CAA64613.1. Sequence problems. AF288176 mRNA. Translation: AAG30125.2. AC005309 Genomic DNA. Translation: AAC63629.2. CP002685 Genomic DNA. Translation: AEC10880.1. DQ069797 mRNA. Translation: AAY82256.1.
IPI	IPI00536062.
PIR	H84918.
RefSeq	NP_850479.1. NM_180148.4.
UniGene	At.25017.
3D structure databases
ProteinModelPortal	Q96266.
SMR	Q96266. Positions 52-257.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96266. 2 interactions.
STRING	3702.AT2G47730.1-P.
Proteomic databases
PaxDb	Q96266.
PRIDE	Q96266.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G47730.1; AT2G47730.1; AT2G47730.
GeneID	819386.
KEGG	ath:AT2G47730.
Organism-specific databases
TAIR	At2g47730.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125746.
InParanoid	Q96266.
KO	K00799.
OMA	LAFERVF.
PhylomeDB	Q96266.
ProtClustDB	CLSN2918292.
Enzyme and pathway databases
BioCyc	ARA:AT2G47730-MONOMER. MetaCyc:AT2G47730-MONOMER.
Gene expression databases
ArrayExpress	Q96266.
Genevestigator	Q96266.
GermOnline	AT2G47730. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF8_ARATH

Accession

Primary (citable) accession number: Q96266
Secondary accession number(s): O82242, Q4PL91, Q9FUT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program