Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanosine nucleotide diphosphate dissociation inhibitor 1

Gene

GDI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-ATH-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine nucleotide diphosphate dissociation inhibitor 1
Short name:
AtGDI1
Gene namesi
Name:GDI1
Ordered Locus Names:At2g44100
ORF Names:F6E13.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G44100.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Guanosine nucleotide diphosphate dissociation inhibitor 1PRO_0000425807Add
BLAST

Proteomic databases

PaxDbiQ96254.
PRIDEiQ96254.

Expressioni

Tissue specificityi

Expressed in roots, rosette leaves, stems, floral buds and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ96254. baseline and differential.
GenevisibleiQ96254. AT.

Interactioni

Subunit structurei

Interacts with the GDP-bound form of RABA5C (via C-terminus).1 Publication

Protein-protein interaction databases

BioGridi4352. 4 interactions.
IntActiQ96254. 1 interaction.
STRINGi3702.AT2G44100.1.

Structurei

3D structure databases

ProteinModelPortaliQ96254.
SMRiQ96254. Positions 1-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Rab GDI family.Curated

Phylogenomic databases

eggNOGiKOG1439. Eukaryota.
COG5044. LUCA.
HOGENOMiHOG000163825.
KOiK17255.
OMAiHQPAYDT.
PhylomeDBiQ96254.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR018203. GDP_dissociation_inhibitor.
IPR000806. RabGDI.
[Graphical view]
PfamiPF00996. GDI. 1 hit.
[Graphical view]
PRINTSiPR00892. RABGDI.
PR00891. RABGDIREP.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEEYEVIVL GTGLKECILS GLLSVDGLKV LHMDRNDYYG GESTSLNLNQ
60 70 80 90 100
LWKKFRGEEK APAHLGSSRD YNVDMMPKFM MANGKLVRVL IHTDVTKYLS
110 120 130 140 150
FKAVDGSYVF VQGKVQKVPA TPMEALKSPL MGIFEKRRAG KFFSYVQEYD
160 170 180 190 200
EKDPKTHDGM DLRRVTTKDL IAKFGLKEDT IDFIGHAVAL HCNDNHLHQP
210 220 230 240 250
AYDTVMRMKL YAESLARFQG GSPYIYPLYG LGELPQAFAR LSAVYGGTYM
260 270 280 290 300
LNKPECKVEF DEEGKVSGVT SEGETAKCKK VVCDPSYLTN KVRKIGRVAR
310 320 330 340 350
AIAIMSHPIP NTNDSQSVQV ILPQKQLGRK SDMYVFCCSY SHNVAPKGKF
360 370 380 390 400
IAFVSTDAET DNPQTELQPG IDLLGPVDEL FFDIYDRYEP VNEPTLDNCF
410 420 430 440
ISTSYDATTH FDTTVVDVLN MYKLITGKEL DLSVDLNAAS AAEEE
Length:445
Mass (Da):49,819
Last modified:February 1, 1997 - v1
Checksum:i228451B5A55C7545
GO
Isoform 2 (identifier: Q96254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-431: ELD → VMF
     432-445: Missing.

Note: No experimental confirmation available.
Show »
Length:431
Mass (Da):48,439
Checksum:i6AB42AB1FA25554A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331E → K in AAL38263 (PubMed:14593172).Curated
Sequence conflicti338 – 3381C → F in AAM64484 (Ref. 8) Curated
Sequence conflicti430 – 4301L → P in AAL38263 (PubMed:14593172).Curated
Sequence conflicti438 – 4381A → P in CAA69258 (PubMed:9091322).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei429 – 4313ELD → VMF in isoform 2. 1 PublicationVSP_053854
Alternative sequencei432 – 44514Missing in isoform 2. 1 PublicationVSP_053855Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83531 mRNA. Translation: BAA11944.1.
Y07961 mRNA. Translation: CAA69258.1.
AC004005 Genomic DNA. Translation: AAC23429.1.
CP002685 Genomic DNA. Translation: AEC10374.1.
CP002685 Genomic DNA. Translation: AEC10375.1.
AF361801 mRNA. Translation: AAK32814.1.
AY050418 mRNA. Translation: AAK91434.1.
AY065087 mRNA. Translation: AAL38263.1.
AY113036 mRNA. Translation: AAM47344.1.
AK316933 mRNA. Translation: BAH19638.1.
AK226690 mRNA. Translation: BAE98797.1.
AK227643 mRNA. Translation: BAE99631.1.
AY086920 mRNA. Translation: AAM64484.1.
PIRiT00690.
RefSeqiNP_001078054.1. NM_001084585.1. [Q96254-2]
NP_181938.1. NM_129973.2. [Q96254-1]
UniGeneiAt.21525.
At.23961.

Genome annotation databases

EnsemblPlantsiAT2G44100.1; AT2G44100.1; AT2G44100. [Q96254-1]
AT2G44100.2; AT2G44100.2; AT2G44100. [Q96254-2]
GeneIDi819016.
KEGGiath:AT2G44100.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83531 mRNA. Translation: BAA11944.1.
Y07961 mRNA. Translation: CAA69258.1.
AC004005 Genomic DNA. Translation: AAC23429.1.
CP002685 Genomic DNA. Translation: AEC10374.1.
CP002685 Genomic DNA. Translation: AEC10375.1.
AF361801 mRNA. Translation: AAK32814.1.
AY050418 mRNA. Translation: AAK91434.1.
AY065087 mRNA. Translation: AAL38263.1.
AY113036 mRNA. Translation: AAM47344.1.
AK316933 mRNA. Translation: BAH19638.1.
AK226690 mRNA. Translation: BAE98797.1.
AK227643 mRNA. Translation: BAE99631.1.
AY086920 mRNA. Translation: AAM64484.1.
PIRiT00690.
RefSeqiNP_001078054.1. NM_001084585.1. [Q96254-2]
NP_181938.1. NM_129973.2. [Q96254-1]
UniGeneiAt.21525.
At.23961.

3D structure databases

ProteinModelPortaliQ96254.
SMRiQ96254. Positions 1-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4352. 4 interactions.
IntActiQ96254. 1 interaction.
STRINGi3702.AT2G44100.1.

Proteomic databases

PaxDbiQ96254.
PRIDEiQ96254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G44100.1; AT2G44100.1; AT2G44100. [Q96254-1]
AT2G44100.2; AT2G44100.2; AT2G44100. [Q96254-2]
GeneIDi819016.
KEGGiath:AT2G44100.

Organism-specific databases

TAIRiAT2G44100.

Phylogenomic databases

eggNOGiKOG1439. Eukaryota.
COG5044. LUCA.
HOGENOMiHOG000163825.
KOiK17255.
OMAiHQPAYDT.
PhylomeDBiQ96254.

Enzyme and pathway databases

ReactomeiR-ATH-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ96254.

Gene expression databases

ExpressionAtlasiQ96254. baseline and differential.
GenevisibleiQ96254. AT.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR018203. GDP_dissociation_inhibitor.
IPR000806. RabGDI.
[Graphical view]
PfamiPF00996. GDI. 1 hit.
[Graphical view]
PRINTSiPR00892. RABGDI.
PR00891. RABGDIREP.
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase."
    Ueda T., Matsuda N., Anai T., Tsukaya H., Uchimiya H., Nakano A.
    Plant Cell 8:2079-2091(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "At-GDI1 from Arabidopsis thaliana encodes a rab-specific GDP dissociation inhibitor that complements the sec19 mutation of Saccharomyces cerevisiae."
    Zarsky V., Cvrckova F., Bischoff F., Palme K.
    FEBS Lett. 403:303-308(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  8. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. "Modes of interaction between the Arabidopsis Rab protein, Ara4, and its putative regulator molecules revealed by a yeast expression system."
    Ueda T., Matsuda N., Uchimiya H., Nakano A.
    Plant J. 21:341-349(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RABA5C.

Entry informationi

Entry nameiGDI1_ARATH
AccessioniPrimary (citable) accession number: Q96254
Secondary accession number(s): B9DFX1
, Q8LBY8, Q8VZB4, Q96284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.