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Protein

Guanosine nucleotide diphosphate dissociation inhibitor 1

Gene

GDI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-ATH-194840 Rho GTPase cycle
R-ATH-6798695 Neutrophil degranulation
R-ATH-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine nucleotide diphosphate dissociation inhibitor 1
Short name:
AtGDI1
Gene namesi
Name:GDI1
Ordered Locus Names:At2g44100
ORF Names:F6E13.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT2G44100
TAIRilocus:2051839 AT2G44100

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004258071 – 445Guanosine nucleotide diphosphate dissociation inhibitor 1Add BLAST445

Proteomic databases

PaxDbiQ96254
PRIDEiQ96254

PTM databases

iPTMnetiQ96254

Expressioni

Tissue specificityi

Expressed in roots, rosette leaves, stems, floral buds and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ96254 baseline and differential
GenevisibleiQ96254 AT

Interactioni

Subunit structurei

Interacts with the GDP-bound form of RABA5C (via C-terminus).1 Publication

Protein-protein interaction databases

BioGridi4352, 4 interactors
IntActiQ96254, 1 interactor
STRINGi3702.AT2G44100.1

Structurei

3D structure databases

ProteinModelPortaliQ96254
SMRiQ96254
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Rab GDI family.Curated

Phylogenomic databases

eggNOGiKOG1439 Eukaryota
COG5044 LUCA
HOGENOMiHOG000163825
KOiK17255
OMAiEDPATHQ
OrthoDBiEOG093608Q1
PhylomeDBiQ96254

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR018203 GDP_dissociation_inhibitor
IPR000806 RabGDI
PANTHERiPTHR11787 PTHR11787, 1 hit
PfamiView protein in Pfam
PF00996 GDI, 1 hit
PRINTSiPR00892 RABGDI
PR00891 RABGDIREP
SUPFAMiSSF51905 SSF51905, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEEYEVIVL GTGLKECILS GLLSVDGLKV LHMDRNDYYG GESTSLNLNQ
60 70 80 90 100
LWKKFRGEEK APAHLGSSRD YNVDMMPKFM MANGKLVRVL IHTDVTKYLS
110 120 130 140 150
FKAVDGSYVF VQGKVQKVPA TPMEALKSPL MGIFEKRRAG KFFSYVQEYD
160 170 180 190 200
EKDPKTHDGM DLRRVTTKDL IAKFGLKEDT IDFIGHAVAL HCNDNHLHQP
210 220 230 240 250
AYDTVMRMKL YAESLARFQG GSPYIYPLYG LGELPQAFAR LSAVYGGTYM
260 270 280 290 300
LNKPECKVEF DEEGKVSGVT SEGETAKCKK VVCDPSYLTN KVRKIGRVAR
310 320 330 340 350
AIAIMSHPIP NTNDSQSVQV ILPQKQLGRK SDMYVFCCSY SHNVAPKGKF
360 370 380 390 400
IAFVSTDAET DNPQTELQPG IDLLGPVDEL FFDIYDRYEP VNEPTLDNCF
410 420 430 440
ISTSYDATTH FDTTVVDVLN MYKLITGKEL DLSVDLNAAS AAEEE
Length:445
Mass (Da):49,819
Last modified:February 1, 1997 - v1
Checksum:i228451B5A55C7545
GO
Isoform 2 (identifier: Q96254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-431: ELD → VMF
     432-445: Missing.

Note: No experimental confirmation available.
Show »
Length:431
Mass (Da):48,439
Checksum:i6AB42AB1FA25554A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233E → K in AAL38263 (PubMed:14593172).Curated1
Sequence conflicti338C → F in AAM64484 (Ref. 8) Curated1
Sequence conflicti430L → P in AAL38263 (PubMed:14593172).Curated1
Sequence conflicti438A → P in CAA69258 (PubMed:9091322).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053854429 – 431ELD → VMF in isoform 2. 1 Publication3
Alternative sequenceiVSP_053855432 – 445Missing in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83531 mRNA Translation: BAA11944.1
Y07961 mRNA Translation: CAA69258.1
AC004005 Genomic DNA Translation: AAC23429.1
CP002685 Genomic DNA Translation: AEC10374.1
CP002685 Genomic DNA Translation: AEC10375.1
AF361801 mRNA Translation: AAK32814.1
AY050418 mRNA Translation: AAK91434.1
AY065087 mRNA Translation: AAL38263.1
AY113036 mRNA Translation: AAM47344.1
AK316933 mRNA Translation: BAH19638.1
AK226690 mRNA Translation: BAE98797.1
AK227643 mRNA Translation: BAE99631.1
AY086920 mRNA Translation: AAM64484.1
PIRiT00690
RefSeqiNP_001078054.1, NM_001084585.2 [Q96254-2]
NP_181938.1, NM_129973.3 [Q96254-1]
UniGeneiAt.21525
At.23961

Genome annotation databases

EnsemblPlantsiAT2G44100.1; AT2G44100.1; AT2G44100 [Q96254-1]
AT2G44100.2; AT2G44100.2; AT2G44100 [Q96254-2]
GeneIDi819016
GrameneiAT2G44100.1; AT2G44100.1; AT2G44100 [Q96254-1]
AT2G44100.2; AT2G44100.2; AT2G44100 [Q96254-2]
KEGGiath:AT2G44100

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiGDI1_ARATH
AccessioniPrimary (citable) accession number: Q96254
Secondary accession number(s): B9DFX1
, Q8LBY8, Q8VZB4, Q96284
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: February 1, 1997
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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