Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Allene oxide synthase, chloroplastic

Gene

CYP74A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(9Z,11E,15Z)-(13S)-hydroperoxyoctadeca-9,11,15-trienoate = (9Z,15Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O.1 Publication

Cofactori

heme1 Publication

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei321Substrate1 Publication1
Binding sitei389Substrate1 Publication1
Metal bindingi471Iron (heme axial ligand)1 Publication1

GO - Molecular functioni

GO - Biological processi

  • defense response Source: TAIR
  • defense response to fungus Source: TAIR
  • epoxygenase P450 pathway Source: TAIR
  • fatty acid biosynthetic process Source: UniProtKB-KW
  • jasmonic acid biosynthetic process Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • oxylipin metabolic process Source: TAIR
  • response to fungus Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: TAIR
  • sterol metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1582.
BRENDAi4.2.1.92. 399.
SABIO-RKQ96242.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Allene oxide synthase, chloroplastic (EC:4.2.1.921 Publication)
Alternative name(s):
Cytochrome P450 74A
Hydroperoxide dehydrase
Gene namesi
Name:CYP74A
Synonyms:AOS
Ordered Locus Names:At5g42650
ORF Names:MJB21.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42650.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plastoglobule Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi137F → L: Impaired allene oxide synthase (AOS) activity, but some hydroperoxide lyase (HPL) activity. Strong hydroperoxide lyase (HPL) activity; when associated with A-155. 1 Publication1
Mutagenesisi155S → A: Strong HPL activity; when associated with L-137. 1 Publication1
Mutagenesisi321N → Q: Abolished allene oxide synthase activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2268010.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Chloroplast1 PublicationAdd BLAST33
ChainiPRO_000000362534 – 518Allene oxide synthase, chloroplasticAdd BLAST485

Proteomic databases

PaxDbiQ96242.
PRIDEiQ96242.

PTM databases

iPTMnetiQ96242.

Expressioni

Inductioni

By wounding.1 Publication

Gene expression databases

GenevisibleiQ96242. AT.

Interactioni

Protein-protein interaction databases

BioGridi19523. 2 interactors.
IntActiQ96242. 1 interactor.
STRINGi3702.AT5G42650.1.

Structurei

Secondary structure

1518
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi66 – 76Combined sources11
Helixi81 – 92Combined sources12
Beta strandi95 – 100Combined sources6
Turni105 – 107Combined sources3
Beta strandi112 – 116Combined sources5
Turni119 – 121Combined sources3
Helixi122 – 126Combined sources5
Turni128 – 130Combined sources3
Beta strandi139 – 141Combined sources3
Helixi145 – 148Combined sources4
Helixi154 – 156Combined sources3
Helixi162 – 177Combined sources16
Turni178 – 181Combined sources4
Helixi182 – 204Combined sources23
Beta strandi205 – 209Combined sources5
Helixi210 – 226Combined sources17
Helixi230 – 232Combined sources3
Turni234 – 237Combined sources4
Helixi239 – 251Combined sources13
Helixi252 – 254Combined sources3
Helixi261 – 268Combined sources8
Beta strandi269 – 271Combined sources3
Helixi275 – 278Combined sources4
Helixi279 – 292Combined sources14
Helixi294 – 302Combined sources9
Helixi307 – 319Combined sources13
Helixi322 – 340Combined sources19
Helixi342 – 358Combined sources17
Turni359 – 361Combined sources3
Helixi365 – 369Combined sources5
Helixi372 – 384Combined sources13
Beta strandi390 – 397Combined sources8
Beta strandi399 – 402Combined sources4
Beta strandi407 – 410Combined sources4
Beta strandi415 – 419Combined sources5
Helixi420 – 424Combined sources5
Turni427 – 429Combined sources3
Turni431 – 434Combined sources4
Turni438 – 441Combined sources4
Helixi443 – 448Combined sources6
Helixi449 – 452Combined sources4
Helixi474 – 491Combined sources18
Beta strandi492 – 501Combined sources10
Beta strandi503 – 515Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RCHX-ray1.85A/B34-518[»]
2RCLX-ray2.41A/B34-518[»]
2RCMX-ray1.73A/B34-518[»]
3CLIX-ray1.80A/B34-518[»]
3DSIX-ray1.60A/B34-518[»]
3DSJX-ray1.60A/B34-518[»]
3DSKX-ray1.55A/B34-518[»]
ProteinModelPortaliQ96242.
SMRiQ96242.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96242.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IF79. Eukaryota.
ENOG410ZAGU. LUCA.
HOGENOMiHOG000238722.
InParanoidiQ96242.
KOiK01723.
OMAiFNFLARA.
OrthoDBiEOG09360850.
PhylomeDBiQ96242.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASISTPFPI SLHPKTVRSK PLKFRVLTRP IKASGSETPD LTVATRTGSK
60 70 80 90 100
DLPIRNIPGN YGLPIVGPIK DRWDYFYDQG AEEFFKSRIR KYNSTVYRVN
110 120 130 140 150
MPPGAFIAEN PQVVALLDGK SFPVLFDVDK VEKKDLFTGT YMPSTELTGG
160 170 180 190 200
YRILSYLDPS EPKHEKLKNL LFFLLKSSRN RIFPEFQATY SELFDSLEKE
210 220 230 240 250
LSLKGKADFG GSSDGTAFNF LARAFYGTNP ADTKLKADAP GLITKWVLFN
260 270 280 290 300
LHPLLSIGLP RVIEEPLIHT FSLPPALVKS DYQRLYEFFL ESAGEILVEA
310 320 330 340 350
DKLGISREEA THNLLFATCF NTWGGMKILF PNMVKRIGRA GHQVHNRLAE
360 370 380 390 400
EIRSVIKSNG GELTMGAIEK MELTKSVVYE CLRFEPPVTA QYGRAKKDLV
410 420 430 440 450
IESHDAAFKV KAGEMLYGYQ PLATRDPKIF DRADEFVPER FVGEEGEKLL
460 470 480 490 500
RHVLWSNGPE TETPTVGNKQ CAGKDFVVLV ARLFVIEIFR RYDSFDIEVG
510
TSPLGSSVNF SSLRKASF
Length:518
Mass (Da):58,197
Last modified:April 27, 2001 - v3
Checksum:iDCC30E428B4A9132
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201 – 233LSLKG…NPADT → AFPLRESGFRRFQRRNRLLF LGSSFLRDESRRY in CAA63266 (PubMed:8756596).CuratedAdd BLAST33
Sequence conflicti289 – 293FLESA → LRIR in CAA63266 (PubMed:8756596).Curated5
Sequence conflicti319C → S in CAA63266 (PubMed:8756596).Curated1
Sequence conflicti339 – 340RA → PG in CAA63266 (PubMed:8756596).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92510 mRNA. Translation: CAA63266.1.
Y12636 Genomic DNA. Translation: CAA73184.1.
AF172727 mRNA. Translation: AAF00225.1.
AB007647 Genomic DNA. Translation: BAB10621.1.
CP002688 Genomic DNA. Translation: AED94842.1.
AY062828 mRNA. Translation: AAL32906.1.
AY065089 mRNA. Translation: AAL38265.1.
AY128733 mRNA. Translation: AAM91133.1.
AY128755 mRNA. Translation: AAM91155.1.
RefSeqiNP_199079.1. NM_123629.4.
UniGeneiAt.211.

Genome annotation databases

EnsemblPlantsiAT5G42650.1; AT5G42650.1; AT5G42650.
GeneIDi834273.
GrameneiAT5G42650.1; AT5G42650.1; AT5G42650.
KEGGiath:AT5G42650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92510 mRNA. Translation: CAA63266.1.
Y12636 Genomic DNA. Translation: CAA73184.1.
AF172727 mRNA. Translation: AAF00225.1.
AB007647 Genomic DNA. Translation: BAB10621.1.
CP002688 Genomic DNA. Translation: AED94842.1.
AY062828 mRNA. Translation: AAL32906.1.
AY065089 mRNA. Translation: AAL38265.1.
AY128733 mRNA. Translation: AAM91133.1.
AY128755 mRNA. Translation: AAM91155.1.
RefSeqiNP_199079.1. NM_123629.4.
UniGeneiAt.211.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RCHX-ray1.85A/B34-518[»]
2RCLX-ray2.41A/B34-518[»]
2RCMX-ray1.73A/B34-518[»]
3CLIX-ray1.80A/B34-518[»]
3DSIX-ray1.60A/B34-518[»]
3DSJX-ray1.60A/B34-518[»]
3DSKX-ray1.55A/B34-518[»]
ProteinModelPortaliQ96242.
SMRiQ96242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19523. 2 interactors.
IntActiQ96242. 1 interactor.
STRINGi3702.AT5G42650.1.

Chemistry databases

ChEMBLiCHEMBL2268010.

PTM databases

iPTMnetiQ96242.

Proteomic databases

PaxDbiQ96242.
PRIDEiQ96242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42650.1; AT5G42650.1; AT5G42650.
GeneIDi834273.
GrameneiAT5G42650.1; AT5G42650.1; AT5G42650.
KEGGiath:AT5G42650.

Organism-specific databases

TAIRiAT5G42650.

Phylogenomic databases

eggNOGiENOG410IF79. Eukaryota.
ENOG410ZAGU. LUCA.
HOGENOMiHOG000238722.
InParanoidiQ96242.
KOiK01723.
OMAiFNFLARA.
OrthoDBiEOG09360850.
PhylomeDBiQ96242.

Enzyme and pathway databases

UniPathwayiUPA00382.
BioCyciMetaCyc:MONOMER-1582.
BRENDAi4.2.1.92. 399.
SABIO-RKQ96242.

Miscellaneous databases

EvolutionaryTraceiQ96242.
PROiQ96242.

Gene expression databases

GenevisibleiQ96242. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCP74A_ARATH
AccessioniPrimary (citable) accession number: Q96242
Secondary accession number(s): P93720, Q9ZR51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 27, 2001
Last modified: November 30, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.