Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kinesin-like protein Klp10A

Gene

Klp10A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux" (PubMed:14681690, PubMed:20946984, PubMed:24100293). Involved in asymmetric cell division of sensory organ precursor (SOP) cells by playing a role in the asymmetric localization of Sara-expressing endosomes to the pIIa daughter cell but not to the pIIb cell. Klp98A targets Sara-expressing endosomes to the central spindle which is symmetrically arranged in early cell division. During late cytokinesis, central spindle asymmetry is generated by enrichment of Patronin on the pIIb side which protects microtubules from depolymerization by Klp10A while unprotected microtubules on the pIIa side are disassembled by Klp10A, leading to the asymmetric delivery of Sara-expressing endosomes to the pIIa daughter cell (PubMed:26659188).4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi368 – 3758ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: FlyBase
  • motor activity Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome duplication Source: FlyBase
  • establishment of meiotic spindle orientation Source: FlyBase
  • meiotic spindle organization Source: FlyBase
  • microtubule depolymerization Source: FlyBase
  • mitotic chromosome movement towards spindle pole Source: FlyBase
  • mitotic spindle assembly Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • spindle assembly involved in female meiosis I Source: FlyBase
  • spindle organization Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-DME-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein Klp10A
Alternative name(s):
Kinesin-like protein at cytological position 10A
Gene namesi
Name:Klp10A
ORF Names:CG1453
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030268. Klp10A.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: FlyBase
  • chromosome, centromeric region Source: FlyBase
  • cortical microtubule Source: FlyBase
  • kinesin complex Source: GO_Central
  • kinetochore microtubule Source: UniProtKB
  • meiotic spindle Source: FlyBase
  • meiotic spindle pole Source: FlyBase
  • mitotic spindle astral microtubule Source: FlyBase
  • mitotic spindle pole Source: FlyBase
  • spindle pole Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Marked perturbation of mitotic spindle architecture.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Kinesin-like protein Klp10APRO_0000125424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571Phosphoserine1 Publication
Modified residuei630 – 6301Phosphothreonine1 Publication
Modified residuei795 – 7951Phosphoserine1 Publication
Modified residuei797 – 7971Phosphoserine2 Publications
Modified residuei800 – 8001Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ960Z0.
PRIDEiQ960Z0.

PTM databases

iPTMnetiQ960Z0.

Expressioni

Gene expression databases

BgeeiFBgn0030268.
ExpressionAtlasiQ960Z0. differential.
GenevisibleiQ960Z0. DM.

Interactioni

Protein-protein interaction databases

BioGridi58463. 1 interaction.
IntActiQ960Z0. 1 interaction.
MINTiMINT-886862.
STRINGi7227.FBpp0073333.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J2Uelectron microscopy10.80K279-615[»]
ProteinModelPortaliQ960Z0.
SMRiQ960Z0. Positions 248-611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini278 – 610333Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 274274GlobularSequence analysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili205 – 24440Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi676 – 68914Asn-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0246. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00830000128292.
HOGENOMiHOG000021111.
InParanoidiQ960Z0.
KOiK10393.
OMAiCITVEWY.
OrthoDBiEOG091G06V3.
PhylomeDBiQ960Z0.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q960Z0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMITVGQSV KIKRTDGRVH MAVVAVINQS GKCITVEWYE RGETKGKEVE
60 70 80 90 100
LDAILTLNPE LMQDTVEQHA APEPKKQATA PMNLSRNPTQ SAIGGNLTSR
110 120 130 140 150
MTMAGNMLNK IQESQSIPNP IVSSNSVNTN SNSNTTAGGG GGTTTSTTTG
160 170 180 190 200
LQRPRYSQAA TGQQQTRIAS AVPNNTLPNP SAAASAGPAA QGVATAATTQ
210 220 230 240 250
GAGGASTRRS HALKEVERLK ENREKRRARQ AEMKEEKVAL MNQDPGNPNW
260 270 280 290 300
ETAQMIREYQ STLEFVPLLD GQAVDDHQIT VCVRKRPISR KEVNRKEIDV
310 320 330 340 350
ISVPRKDMLI VHEPRSKVDL TKFLENHKFR FDYAFNDTCD NAMVYKYTAK
360 370 380 390 400
PLVKTIFEGG MATCFAYGQT GSGKTHTMGG EFNGKVQDCK NGIYAMAAKD
410 420 430 440 450
VFVTLNMPRY RAMNLVVSAS FFEIYSGKVF DLLSDKQKLR VLEDGKQQVQ
460 470 480 490 500
VVGLTEKVVD GVEEVLKLIQ HGNAARTSGQ TSANSNSSRS HAVFQIVLRP
510 520 530 540 550
QGSTKIHGKF SFIDLAGNER GVDTSSADRQ TRMEGAEINK SLLALKECIR
560 570 580 590 600
ALGKQSAHLP FRVSKLTQVL RDSFIGEKSK TCMIAMISPG LSSCEHTLNT
610 620 630 640 650
LRYADRVKEL VVKDIVEVCP GGDTEPIEIT DDEEEEELNM VHPHSHQLHP
660 670 680 690 700
NSHAPASQSN NQRAPASHHS GAVIHNNNNN NNKNGNAGNM DLAMLSSLSE
710 720 730 740 750
HEMSDELIVQ HQAIDDLQQT EEMVVEYHRT VNATLETFLA ESKALYNLTN
760 770 780 790 800
YVDYDQDSYC KRGESMFSQL LDIAIQCRDM MAEYRAKLAK EEMLSCSFNS

PNGKR
Length:805
Mass (Da):88,672
Last modified:December 1, 2001 - v1
Checksum:i1667DE7C5478138D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAN09282.1.
AY051763 mRNA. Translation: AAK93187.1.
RefSeqiNP_001285109.1. NM_001298180.1.
NP_572687.1. NM_132459.4.
NP_727491.1. NM_167267.2.
NP_727492.1. NM_167268.2.
NP_727493.1. NM_167269.2.
NP_727494.1. NM_167270.2.
UniGeneiDm.130.

Genome annotation databases

EnsemblMetazoaiFBtr0073475; FBpp0073331; FBgn0030268.
FBtr0073476; FBpp0073332; FBgn0030268.
FBtr0073477; FBpp0073333; FBgn0030268.
FBtr0073478; FBpp0073334; FBgn0030268.
FBtr0073479; FBpp0073335; FBgn0030268.
FBtr0346270; FBpp0312027; FBgn0030268.
GeneIDi32049.
KEGGidme:Dmel_CG1453.
UCSCiCG1453-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAN09282.1.
AY051763 mRNA. Translation: AAK93187.1.
RefSeqiNP_001285109.1. NM_001298180.1.
NP_572687.1. NM_132459.4.
NP_727491.1. NM_167267.2.
NP_727492.1. NM_167268.2.
NP_727493.1. NM_167269.2.
NP_727494.1. NM_167270.2.
UniGeneiDm.130.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J2Uelectron microscopy10.80K279-615[»]
ProteinModelPortaliQ960Z0.
SMRiQ960Z0. Positions 248-611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58463. 1 interaction.
IntActiQ960Z0. 1 interaction.
MINTiMINT-886862.
STRINGi7227.FBpp0073333.

PTM databases

iPTMnetiQ960Z0.

Proteomic databases

PaxDbiQ960Z0.
PRIDEiQ960Z0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073475; FBpp0073331; FBgn0030268.
FBtr0073476; FBpp0073332; FBgn0030268.
FBtr0073477; FBpp0073333; FBgn0030268.
FBtr0073478; FBpp0073334; FBgn0030268.
FBtr0073479; FBpp0073335; FBgn0030268.
FBtr0346270; FBpp0312027; FBgn0030268.
GeneIDi32049.
KEGGidme:Dmel_CG1453.
UCSCiCG1453-RA. d. melanogaster.

Organism-specific databases

CTDi32049.
FlyBaseiFBgn0030268. Klp10A.

Phylogenomic databases

eggNOGiKOG0246. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00830000128292.
HOGENOMiHOG000021111.
InParanoidiQ960Z0.
KOiK10393.
OMAiCITVEWY.
OrthoDBiEOG091G06V3.
PhylomeDBiQ960Z0.

Enzyme and pathway databases

ReactomeiR-DME-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-DME-983189. Kinesins.

Miscellaneous databases

GenomeRNAii32049.
PROiQ960Z0.

Gene expression databases

BgeeiFBgn0030268.
ExpressionAtlasiQ960Z0. differential.
GenevisibleiQ960Z0. DM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKI10A_DROME
AccessioniPrimary (citable) accession number: Q960Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.