Q960Z0 (KI10A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein Klp10A

Alternative name(s):
Kinesin-like protein at cytological position 10A

Gene names

Name:	Klp10A
ORF Names:	CG1453

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux". Ref.4
Subcellular location	Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle pole. Chromosome › centromere. Note: Localizes to mitotic centrosomes, spindle poles and centromeres through metaphase. However, the centromeric localization diminishes markedly at the onset of anaphase, leaving the majority of the protein on the spindle poles. Concentrated around the minus ends focused at the poles. Ref.4
Disruption phenotype	Marked perturbation of mitotic spindle architecture. Ref.4
Sequence similarities	Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW microtubule-based movement Inferred from electronic annotation. Source: InterPro mitotic anaphase A Inferred from direct assay. Source: FlyBase mitotic spindle organization Inferred from mutant phenotype. Source: FlyBase
Cellular component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell kinetochore microtubule Inferred from direct assay Ref.4. Source: UniProtKB microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole Inferred from direct assay. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 805	805	Kinesin-like protein Klp10A		PRO_0000125424
Regions
	Domain	275 – 540	266	Kinesin-motor
	Nucleotide binding	368 – 375	8	ATP By similarity
	Region	1 – 274	274	Globular Potential
	Coiled coil	205 – 244	40	Potential
	Compositional bias	676 – 689	14	Asn-rich
Amino acid modifications
	Modified residue	157	1	Phosphoserine Ref.6
	Modified residue	630	1	Phosphothreonine Ref.6
	Modified residue	795	1	Phosphoserine Ref.6
	Modified residue	797	1	Phosphoserine Ref.5 Ref.6
	Modified residue	800	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q960Z0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 1667DE7C5478138D	FASTA	805	88,672
	10 20 30 40 50 60 MDMITVGQSV KIKRTDGRVH MAVVAVINQS GKCITVEWYE RGETKGKEVE LDAILTLNPE 70 80 90 100 110 120 LMQDTVEQHA APEPKKQATA PMNLSRNPTQ SAIGGNLTSR MTMAGNMLNK IQESQSIPNP 130 140 150 160 170 180 IVSSNSVNTN SNSNTTAGGG GGTTTSTTTG LQRPRYSQAA TGQQQTRIAS AVPNNTLPNP 190 200 210 220 230 240 SAAASAGPAA QGVATAATTQ GAGGASTRRS HALKEVERLK ENREKRRARQ AEMKEEKVAL 250 260 270 280 290 300 MNQDPGNPNW ETAQMIREYQ STLEFVPLLD GQAVDDHQIT VCVRKRPISR KEVNRKEIDV 310 320 330 340 350 360 ISVPRKDMLI VHEPRSKVDL TKFLENHKFR FDYAFNDTCD NAMVYKYTAK PLVKTIFEGG 370 380 390 400 410 420 MATCFAYGQT GSGKTHTMGG EFNGKVQDCK NGIYAMAAKD VFVTLNMPRY RAMNLVVSAS 430 440 450 460 470 480 FFEIYSGKVF DLLSDKQKLR VLEDGKQQVQ VVGLTEKVVD GVEEVLKLIQ HGNAARTSGQ 490 500 510 520 530 540 TSANSNSSRS HAVFQIVLRP QGSTKIHGKF SFIDLAGNER GVDTSSADRQ TRMEGAEINK 550 560 570 580 590 600 SLLALKECIR ALGKQSAHLP FRVSKLTQVL RDSFIGEKSK TCMIAMISPG LSSCEHTLNT 610 620 630 640 650 660 LRYADRVKEL VVKDIVEVCP GGDTEPIEIT DDEEEEELNM VHPHSHQLHP NSHAPASQSN 670 680 690 700 710 720 NQRAPASHHS GAVIHNNNNN NNKNGNAGNM DLAMLSSLSE HEMSDELIVQ HQAIDDLQQT 730 740 750 760 770 780 EEMVVEYHRT VNATLETFLA ESKALYNLTN YVDYDQDSYC KRGESMFSQL LDIAIQCRDM 790 800 MAEYRAKLAK EEMLSCSFNS PNGKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase." Rogers G.C., Rogers S.L., Schwimmer T.A., Ems-McClung S.C., Walczak C.E., Vale R.D., Scholey J.M., Sharp D.J. Nature 427:364-370(2004) [PubMed: 14681690] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[5]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-797, MASS SPECTROMETRY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; THR-630; SER-795; SER-797 AND SER-800, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAN09282.1. AY051763 mRNA. Translation: AAK93187.1.
RefSeq	NP_572687.1. NM_132459.2. NP_727491.1. NM_167267.1. NP_727492.1. NM_167268.1. NP_727493.1. NM_167269.1. NP_727494.1. NM_167270.1.
UniGene	Dm.130.
3D structure databases
ProteinModelPortal	Q960Z0.
SMR	Q960Z0. Positions 210-610.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-886862.
STRING	Q960Z0.
Proteomic databases
PRIDE	Q960Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073475; FBpp0073331; FBgn0030268. FBtr0073476; FBpp0073332; FBgn0030268. FBtr0073477; FBpp0073333; FBgn0030268. FBtr0073478; FBpp0073334; FBgn0030268. FBtr0073479; FBpp0073335; FBgn0030268.
GeneID	32049.
KEGG	dme:Dmel_CG1453.
UCSC	CG1453-RA. d. melanogaster.
Organism-specific databases
CTD	32049.
FlyBase	FBgn0030268. Klp10A.
Phylogenomic databases
eggNOG	inNOG06851.
GeneTree	EMGT00050000000751.
InParanoid	Q960Z0.
OMA	FRVSKLT.
OrthoDB	EOG4P8D0F.
PhylomeDB	Q960Z0.
Gene expression databases
Bgee	Q960Z0.
GermOnline	CG1453. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 1 hit.
KO	K10393.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	776553.

Entry information

Entry name

KI10A_DROME

Accession

Primary (citable) accession number: Q960Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project