Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q960X4

- TIP60_DROME

UniProt

Q960X4 - TIP60_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Histone acetyltransferase Tip60

Gene

Tip60

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic subunit of the Tip60 chromatin-remodeling complex which is involved in DNA repair. Upon induction of DNA double-strand breaks, acetylates phosphorylated histone H2AV in nucleosomes on 'Lys-4' and exchanges it with unmodified H2AV.1 Publication

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 4311Proton donor/acceptorBy similarity
Binding sitei435 – 4351Acetyl-CoABy similarity
Binding sitei444 – 4441Acetyl-CoABy similarity

GO - Molecular functioni

  1. histone acetyltransferase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: FlyBase
  2. histone acetylation Source: UniProtKB
  3. histone exchange Source: UniProtKB
  4. histone H4 acetylation Source: FlyBase
  5. negative regulation of neuron apoptotic process Source: FlyBase
  6. nervous system development Source: FlyBase
  7. regulation of gene expression Source: FlyBase
  8. regulation of synaptic plasticity Source: FlyBase
  9. regulation of terminal button organization Source: FlyBase
  10. regulation of transcription, DNA-templated Source: UniProtKB-KW
  11. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_218447. formation of the beta-catenin:TCF transactivating complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase Tip60 (EC:2.3.1.481 Publication)
Gene namesi
Name:Tip60
ORF Names:CG6121
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0026080. Tip60.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. histone acetyltransferase complex Source: FlyBase
  2. NuA4 histone acetyltransferase complex Source: UniProtKB
  3. nucleus Source: FlyBase
  4. polytene chromosome interband Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541Histone acetyltransferase Tip60PRO_0000051583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei355 – 3551N6-acetyllysine; by autocatalysisBy similarity

Post-translational modificationi

Autoacetylation at Lys-355 is required for proper function.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ960X4.

Expressioni

Gene expression databases

BgeeiQ960X4.

Interactioni

Subunit structurei

Component of the Tip60 chromatin-remodeling complex which contains the catalytic subunit Tip60 and the subunits Domino, Tra1, Brd8, E(Pc), DMAP1, Pontin, Reptin, Ing3, Act87E, BAP55, Mrg15, MrgBP, Gas41 and YL-1.1 Publication

Protein-protein interaction databases

BioGridi57879. 5 interactions.
IntActiQ960X4. 4 interactions.
MINTiMINT-785367.

Structurei

3D structure databases

ProteinModelPortaliQ960X4.
SMRiQ960X4. Positions 27-96, 258-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini255 – 533279MYST-type HATAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni398 – 4003Acetyl-CoA bindingBy similarity
Regioni405 – 4117Acetyl-CoA bindingBy similarity

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated

Phylogenomic databases

eggNOGiCOG5027.
GeneTreeiENSGT00550000074503.
InParanoidiQ960X4.
KOiK11304.
OMAiKHTISFF.
OrthoDBiEOG7ZKS9R.
PhylomeDBiQ960X4.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q960X4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKINHKYEFD DDVASICEST AALTEGCRLP VRMHKTDDWP LAEIVSIKEL
60 70 80 90 100
DGRRQFYVHY VDFNKRLDEW VNEEDLYTRK VQFPRRDGSQ TGTSTGVTTP
110 120 130 140 150
QRHHSLAGSV SRPTSPQHPG SGALAAIPQT PTGASGSVPP PAGIPNSVAP
160 170 180 190 200
PGTPSSGGEL VNGNNLAAAL QKRINRKRKN HGGSAHGHHS LTSQQQQSHP
210 220 230 240 250
HPTTPQTPTA TPVHVTGDGL ISGAANDDGD GSQDGKTPTP RQSGSMVTHQ
260 270 280 290 300
DDVVTRMKNV EMIELGRHRI KPWYFSPYPQ ELCQMPCIYI CEFCLKYRKS
310 320 330 340 350
RKCLERHLSK CNLRHPPGNE IYRKHTISFF EIDGRKNKVY AQNLCLLAKL
360 370 380 390 400
FLDHKTLYYD TDPFLFYVMT EFDSRGFHIV GYFSKEKEST EDYNVACILT
410 420 430 440 450
MPPYQRKGYG KLLIEFSYEL SKFEGKTGSP EKPLSDLGLL SYRSYWAQTI
460 470 480 490 500
LEIFISQNPS TDGEKPTITI NDICECTSIK KEDVISTLQN LNLINYYKGQ
510 520 530 540
YIVCINRVII EQHRRAMDKR KIRIDSKCLH WTPKDWSKRS K
Length:541
Mass (Da):61,234
Last modified:December 1, 2001 - v1
Checksum:iF48A3415B708A97C
GO

Sequence cautioni

The sequence CAA21829.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAF45923.3.
AL033125 Genomic DNA. Translation: CAA21829.1. Sequence problems.
AY051795 mRNA. Translation: AAK93219.1.
RefSeqiNP_001259234.1. NM_001272305.1.
NP_572151.1. NM_131923.4.
UniGeneiDm.75.

Genome annotation databases

EnsemblMetazoaiFBtr0070668; FBpp0070636; FBgn0026080.
FBtr0333186; FBpp0305388; FBgn0026080.
GeneIDi31362.
KEGGidme:Dmel_CG6121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAF45923.3 .
AL033125 Genomic DNA. Translation: CAA21829.1 . Sequence problems.
AY051795 mRNA. Translation: AAK93219.1 .
RefSeqi NP_001259234.1. NM_001272305.1.
NP_572151.1. NM_131923.4.
UniGenei Dm.75.

3D structure databases

ProteinModelPortali Q960X4.
SMRi Q960X4. Positions 27-96, 258-533.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 57879. 5 interactions.
IntActi Q960X4. 4 interactions.
MINTi MINT-785367.

Proteomic databases

PRIDEi Q960X4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0070668 ; FBpp0070636 ; FBgn0026080 .
FBtr0333186 ; FBpp0305388 ; FBgn0026080 .
GeneIDi 31362.
KEGGi dme:Dmel_CG6121.

Organism-specific databases

CTDi 31362.
FlyBasei FBgn0026080. Tip60.

Phylogenomic databases

eggNOGi COG5027.
GeneTreei ENSGT00550000074503.
InParanoidi Q960X4.
KOi K11304.
OMAi KHTISFF.
OrthoDBi EOG7ZKS9R.
PhylomeDBi Q960X4.

Enzyme and pathway databases

Reactomei REACT_218447. formation of the beta-catenin:TCF transactivating complex.

Miscellaneous databases

ChiTaRSi KAT5. drosophila.
GenomeRNAii 31362.
NextBioi 773249.
PROi Q960X4.

Gene expression databases

Bgeei Q960X4.

Family and domain databases

Gene3Di 3.40.630.30. 1 hit.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
[Graphical view ]
Pfami PF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view ]
SMARTi SM00298. CHROMO. 1 hit.
[Graphical view ]
SUPFAMi SSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEi PS51726. MYST_HAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Oregon-R.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions."
    Kusch T., Florens L., Macdonald W.H., Swanson S.K., Glaser R.L., Yates J.R. III, Abmayr S.M., Washburn M.P., Workman J.L.
    Science 306:2084-2087(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TIP60 COMPLEX, FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiTIP60_DROME
AccessioniPrimary (citable) accession number: Q960X4
Secondary accession number(s): O97425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3