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Q960X4

- TIP60_DROME

UniProt

Q960X4 - TIP60_DROME

Protein

Histone acetyltransferase Tip60

Gene

Tip60

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the Tip60 chromatin-remodeling complex which is involved in DNA repair. Upon induction of DNA double-strand breaks, acetylates phosphorylated histone H2AV in nucleosomes on 'Lys-4' and exchanges it with unmodified H2AV.1 Publication

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei355 – 3551By similarity
    Active sitei397 – 3971NucleophileBy similarity
    Binding sitei400 – 4001Acetyl-CoABy similarity
    Binding sitei435 – 4351Acetyl-CoABy similarity

    GO - Molecular functioni

    1. histone acetyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: FlyBase
    2. histone acetylation Source: UniProtKB
    3. histone exchange Source: UniProtKB
    4. histone H4 acetylation Source: FlyBase
    5. negative regulation of neuron apoptotic process Source: FlyBase
    6. nervous system development Source: FlyBase
    7. regulation of gene expression Source: FlyBase
    8. regulation of synaptic plasticity Source: FlyBase
    9. regulation of terminal button organization Source: FlyBase
    10. regulation of transcription, DNA-templated Source: UniProtKB-KW
    11. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Chromatin regulator, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_218447. formation of the beta-catenin:TCF transactivating complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone acetyltransferase Tip60 (EC:2.3.1.48)
    Gene namesi
    Name:Tip60
    ORF Names:CG6121
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0026080. Tip60.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. histone acetyltransferase complex Source: FlyBase
    2. NuA4 histone acetyltransferase complex Source: UniProtKB
    3. nucleus Source: FlyBase
    4. polytene chromosome interband Source: FlyBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 541541Histone acetyltransferase Tip60PRO_0000051583Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei355 – 3551N6-acetyllysine; by autocatalysisBy similarity

    Post-translational modificationi

    Autoacetylation at Lys-355 is required for proper function.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ960X4.

    Expressioni

    Gene expression databases

    BgeeiQ960X4.

    Interactioni

    Subunit structurei

    Component of the Tip60 chromatin-remodeling complex which contains the catalytic subunit Tip60 and the subunits Domino, Tra1, Brd8, E(Pc), DMAP1, Pontin, Reptin, Ing3, Act87E, BAP55, Mrg15, MrgBP, Gas41 and YL-1.1 Publication

    Protein-protein interaction databases

    BioGridi57879. 5 interactions.
    IntActiQ960X4. 4 interactions.
    MINTiMINT-785367.

    Structurei

    3D structure databases

    ProteinModelPortaliQ960X4.
    SMRiQ960X4. Positions 27-96, 258-533.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini255 – 533279MYST-type HATAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni405 – 4117Acetyl-CoA bindingBy similarity

    Sequence similaritiesi

    Belongs to the MYST (SAS/MOZ) family.Curated

    Phylogenomic databases

    eggNOGiCOG5027.
    GeneTreeiENSGT00550000074503.
    InParanoidiQ960X4.
    KOiK11304.
    OMAiKHTISFF.
    OrthoDBiEOG7ZKS9R.
    PhylomeDBiQ960X4.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR002717. MOZ_SAS.
    IPR025995. Tudor-knot.
    [Graphical view]
    PfamiPF01853. MOZ_SAS. 1 hit.
    PF11717. Tudor-knot. 1 hit.
    [Graphical view]
    SMARTiSM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF54160. SSF54160. 1 hit.
    SSF55729. SSF55729. 1 hit.
    PROSITEiPS51726. MYST_HAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q960X4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKINHKYEFD DDVASICEST AALTEGCRLP VRMHKTDDWP LAEIVSIKEL    50
    DGRRQFYVHY VDFNKRLDEW VNEEDLYTRK VQFPRRDGSQ TGTSTGVTTP 100
    QRHHSLAGSV SRPTSPQHPG SGALAAIPQT PTGASGSVPP PAGIPNSVAP 150
    PGTPSSGGEL VNGNNLAAAL QKRINRKRKN HGGSAHGHHS LTSQQQQSHP 200
    HPTTPQTPTA TPVHVTGDGL ISGAANDDGD GSQDGKTPTP RQSGSMVTHQ 250
    DDVVTRMKNV EMIELGRHRI KPWYFSPYPQ ELCQMPCIYI CEFCLKYRKS 300
    RKCLERHLSK CNLRHPPGNE IYRKHTISFF EIDGRKNKVY AQNLCLLAKL 350
    FLDHKTLYYD TDPFLFYVMT EFDSRGFHIV GYFSKEKEST EDYNVACILT 400
    MPPYQRKGYG KLLIEFSYEL SKFEGKTGSP EKPLSDLGLL SYRSYWAQTI 450
    LEIFISQNPS TDGEKPTITI NDICECTSIK KEDVISTLQN LNLINYYKGQ 500
    YIVCINRVII EQHRRAMDKR KIRIDSKCLH WTPKDWSKRS K 541
    Length:541
    Mass (Da):61,234
    Last modified:December 1, 2001 - v1
    Checksum:iF48A3415B708A97C
    GO

    Sequence cautioni

    The sequence CAA21829.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF45923.3.
    AL033125 Genomic DNA. Translation: CAA21829.1. Sequence problems.
    AY051795 mRNA. Translation: AAK93219.1.
    RefSeqiNP_001259234.1. NM_001272305.1.
    NP_572151.1. NM_131923.4.
    UniGeneiDm.75.

    Genome annotation databases

    EnsemblMetazoaiFBtr0070668; FBpp0070636; FBgn0026080.
    FBtr0333186; FBpp0305388; FBgn0026080.
    GeneIDi31362.
    KEGGidme:Dmel_CG6121.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF45923.3 .
    AL033125 Genomic DNA. Translation: CAA21829.1 . Sequence problems.
    AY051795 mRNA. Translation: AAK93219.1 .
    RefSeqi NP_001259234.1. NM_001272305.1.
    NP_572151.1. NM_131923.4.
    UniGenei Dm.75.

    3D structure databases

    ProteinModelPortali Q960X4.
    SMRi Q960X4. Positions 27-96, 258-533.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 57879. 5 interactions.
    IntActi Q960X4. 4 interactions.
    MINTi MINT-785367.

    Proteomic databases

    PRIDEi Q960X4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0070668 ; FBpp0070636 ; FBgn0026080 .
    FBtr0333186 ; FBpp0305388 ; FBgn0026080 .
    GeneIDi 31362.
    KEGGi dme:Dmel_CG6121.

    Organism-specific databases

    CTDi 31362.
    FlyBasei FBgn0026080. Tip60.

    Phylogenomic databases

    eggNOGi COG5027.
    GeneTreei ENSGT00550000074503.
    InParanoidi Q960X4.
    KOi K11304.
    OMAi KHTISFF.
    OrthoDBi EOG7ZKS9R.
    PhylomeDBi Q960X4.

    Enzyme and pathway databases

    Reactomei REACT_218447. formation of the beta-catenin:TCF transactivating complex.

    Miscellaneous databases

    ChiTaRSi KAT5. drosophila.
    GenomeRNAii 31362.
    NextBioi 773249.
    PROi Q960X4.

    Gene expression databases

    Bgeei Q960X4.

    Family and domain databases

    Gene3Di 3.40.630.30. 1 hit.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR002717. MOZ_SAS.
    IPR025995. Tudor-knot.
    [Graphical view ]
    Pfami PF01853. MOZ_SAS. 1 hit.
    PF11717. Tudor-knot. 1 hit.
    [Graphical view ]
    SMARTi SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54160. SSF54160. 1 hit.
    SSF55729. SSF55729. 1 hit.
    PROSITEi PS51726. MYST_HAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Oregon-R.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    5. "Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions."
      Kusch T., Florens L., Macdonald W.H., Swanson S.K., Glaser R.L., Yates J.R. III, Abmayr S.M., Washburn M.P., Workman J.L.
      Science 306:2084-2087(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TIP60 COMPLEX, FUNCTION.

    Entry informationi

    Entry nameiTIP60_DROME
    AccessioniPrimary (citable) accession number: Q960X4
    Secondary accession number(s): O97425
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3