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Protein

Trafficking kinesin-binding protein milt

Gene

milt

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for kinesin-mediated axonal transport of mitochondria to nerve terminals. The oocyte acquires the majority of its mitochondria by competitive bidirectional transport along microtubules mediated by the Milton adapter. Mitochondria enter the young oocyte en mass from interconnected germ cells to generate the large aggregate known as the Balbiani body. Milt and Miro form an essential protein complex that links Khc to mitochondria for light chain-independent, anterograde transport of mitochondria.3 Publications

Miscellaneous

Was named 'Milton' after the blind British poet.

GO - Molecular functioni

  • microtubule motor activity Source: UniProtKB
  • myosin binding Source: FlyBase

GO - Biological processi

  • axonal transport of mitochondrion Source: FlyBase
  • establishment of mitochondrion localization Source: UniProtKB
  • mitochondrion distribution Source: FlyBase
  • Nebenkern assembly Source: FlyBase
  • oocyte dorsal/ventral axis specification Source: UniProtKB
  • phototransduction Source: FlyBase
  • sperm mitochondrion organization Source: FlyBase

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking kinesin-binding protein milt1 Publication
Short name:
Protein milton1 Publication
Gene namesi
Name:milt
ORF Names:CG43227
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0262872. milt.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Flies show aberrant synaptic transmission in photoreceptors despite normal phototransduction. Synaptic terminals and axons lack mitochondria, although mitochondria are numerous in neuronal cell bodies. Synaptic vesicles continue to be transported to and concentrated at synapses. In mutant oocytes mitochondria are transported prematurely and excessively.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003511431 – 1122Trafficking kinesin-binding protein miltAdd BLAST1122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei831Phosphoserine1 Publication1
Modified residuei918Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ960V3.
PRIDEiQ960V3.

PTM databases

iPTMnetiQ960V3.

Expressioni

Tissue specificityi

Expressed primarily in axons and synapses.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0262872.
ExpressionAtlasiQ960V3. differential.
GenevisibleiQ960V3. DM.

Interactioni

Subunit structurei

Interacts with Miro. Note=Mitochondrial transport by milt and Khc is independent of Klc.1 Publication

GO - Molecular functioni

  • myosin binding Source: FlyBase

Protein-protein interaction databases

BioGridi69809. 8 interactors.
IntActiQ960V3. 8 interactors.
STRINGi7227.FBpp0297341.

Structurei

3D structure databases

ProteinModelPortaliQ960V3.
SMRiQ960V3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 382HAP1 N-terminalSequence analysisAdd BLAST296

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 450Sufficient for interaction with Khc1 PublicationAdd BLAST450

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili142 – 209Sequence analysisAdd BLAST68
Coiled coili235 – 382Sequence analysisAdd BLAST148

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi854 – 872Gln-richSequence analysisAdd BLAST19

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4360. Eukaryota.
ENOG410XSWD. LUCA.
GeneTreeiENSGT00390000003132.
InParanoidiQ960V3.
KOiK15369.
OMAiQRKVNTL.
OrthoDBiEOG091G0LLD.
PhylomeDBiQ960V3.

Family and domain databases

InterProiView protein in InterPro
IPR006933. HAP1_N.
IPR022154. Traffickng_kinesin-bd_prot_dom.
PfamiView protein in Pfam
PF04849. HAP1_N. 1 hit.
PF12448. Milton. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B1 Publication (identifier: Q960V3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTHVNNGEVM EKEMEPTGER ERDRETAAGS GVHHRFLASA SERDANSGNA
60 70 80 90 100
LEAAATKTKT GTTITNLEDL AFEACQNWSD LHQDFFITDD ELEYEDELSL
110 120 130 140 150
GSSIGGNIAT TTTADAAVEG LITGEHQNEQ LLMEVLCGNR VSQMTRAYDD
160 170 180 190 200
IEAVTRLLEE KEKDLELTVQ IGKELLTQNN ALEARVADLE TDLKASNDDR
210 220 230 240 250
AQLVHELHKK NELISVLTND ADDGTDTDTP TMSKSITLDL LQRKVNSLLD
260 270 280 290 300
ENKSLKCEAT QLAHQTDEVE EHERQLMADI SAQLNDANSQ YDNLSLELER
310 320 330 340 350
QREENRLQHE QIVNLTARLA EAEMRLHQLT QDNDEHLSLL HVTKENQNAL
360 370 380 390 400
ALELVEFKQR YEEVLALLHS AQDQLKQQRK RSQPQARSSF LGGLGTSGAG
410 420 430 440 450
MGGSLFPPDS LHCELMESSL YSENSLDSGI SGDSQRSADR ISRMMMHMPS
460 470 480 490 500
GGMSSSTMGG SVYAGAGNVP PYKRVFDTVR CAGKSGNYMD SGNVSMTQLG
510 520 530 540 550
AMSMSSSSGP RMASMAYPAG SYYRGGSNQS LGVKTLSSES LNSQSDDGYP
560 570 580 590 600
AQPSGVPGAP GAKELEAALK RLTPAEVLAR RAMLSYAPAG TYNYDEPMGH
610 620 630 640 650
GTGNVRNSDL PLGVRTPDSI MSTGSSGMSG STNHMSASMT HQWRLPEKLQ
660 670 680 690 700
IIKPMEGSQT LHHWSRLATP TLSGLLDERP GVTIRGGRGL DDLGMQIYTL
710 720 730 740 750
SDVEEDVSDD LPGKQFEAPG CTFTYTNSMV MHPDDGFVND LSFLSQSQMS
760 770 780 790 800
SRMASTSTSR QPSCPATPRA GLSRKNSCST FSVNLGLAGM LNERGIKAVT
810 820 830 840 850
PSALNTPAGP NFSPTVTPCN SPEGSPPRAQ SPEPLFGLLS CGADLIRRKI
860 870 880 890 900
VGDQHQQQQQ KQRSSLSKQQ QQKIMLSHLE RRALRSLNLI EKVESIGLEN
910 920 930 940 950
IISAQRGLGS GIANRSSSPL SSGSLQSLHT SSNSIVDDIH FDRAQIKGVL
960 970 980 990 1000
HRGLKSPTPA TASTSAAAAA SGPGISTSSS TSAYNSDDSD DQGLVMKIKP
1010 1020 1030 1040 1050
SKSATPTTTG AAQSQPSSAT TSNGTASETR LKQMQRQKSR RQLKNGMANQ
1060 1070 1080 1090 1100
RPDLGTISGA GGGGRVRPDL GKVADSGSSS KLSTKRSEAK PAEEEEATPQ
1110 1120
TITQAFVGSV SSLLFGRKGG WL
Length:1,122
Mass (Da):120,649
Last modified:December 1, 2001 - v1
Checksum:i7BBE5E474C8112BC
GO
Isoform A1 Publication (identifier: Q960V3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: MTHVNNGEVM...HQNEQLLMEV → MLSATLGANG...QTRECLNYFL

Show »
Length:1,116
Mass (Da):120,167
Checksum:iCEE9CE7E2E1814F8
GO
Isoform C1 Publication (identifier: Q960V3-3)
Sequence is not available
Length:
Mass (Da):
Isoform D1 Publication (identifier: Q960V3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.

Show »
Length:979
Mass (Da):105,149
Checksum:iCBC1ADE45C3ABACD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1098T → P in AAK74155 (PubMed:12495622).Curated1
Sequence conflicti1098T → P in AAK74156 (PubMed:12495622).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0529341 – 143Missing in isoform D. 1 PublicationAdd BLAST143
Alternative sequenceiVSP_0529331 – 135MTHVN…LLMEV → MLSATLGANGGSQPLSPSAQ ATLSKHLPRLQSKRLLQQTQ LQSPAAARPQTCDASCLTEL CSSENLPEVEIFSLLEEQIP KYKVRNDFLTNFSGYANEDW FVPAPALPIPPEGLGLTKEQ TRECLNYFL in isoform A. 2 PublicationsAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY038000 mRNA. Translation: AAK74155.1.
AY038001 mRNA. Translation: AAK74156.1.
AE014134 Genomic DNA. Translation: AAN10620.1.
AE014134 Genomic DNA. Translation: AAN10622.1.
AY051825 mRNA. Translation: AAK93249.1.
AY095025 mRNA. Translation: AAM11353.1.
RefSeqiNP_542943.2. NM_080765.4. [Q960V3-2]
NP_723249.1. NM_164736.3. [Q960V3-2]
NP_723251.1. NM_164738.3. [Q960V3-1]
UniGeneiDm.7402.

Genome annotation databases

EnsemblMetazoaiFBtr0306228; FBpp0297338; FBgn0262872. [Q960V3-2]
FBtr0306229; FBpp0297339; FBgn0262872. [Q960V3-2]
FBtr0306230; FBpp0297340; FBgn0262872. [Q960V3-1]
FBtr0306232; FBpp0297342; FBgn0262872. [Q960V3-4]
GeneIDi45683.
KEGGidme:Dmel_CG43227.
UCSCiCG13777-RA. d. melanogaster.
CG13777-RB. d. melanogaster. [Q960V3-1]
CG13777-RC. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMILT_DROME
AccessioniPrimary (citable) accession number: Q960V3
Secondary accession number(s): Q0E8S6, Q8SWX2, Q95NI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: December 1, 2001
Last modified: October 25, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase