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Q960T2 (SAP47_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synapse-associated protein of 47 kDa
Gene names
Name:Sap47
ORF Names:CG8884
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Tissue specificity

Expressed specifically in neurons and transported to synaptic terminals. Ref.1

Sequence similarities

Contains 1 BSD domain.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q960T2-1)

Also known as: C; D; E; H;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform B (identifier: Q960T2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     370-374: NEVAT → LAFNY
     375-551: Missing.
Note: No experimental confirmation available.
Isoform F (identifier: Q960T2-4)

Also known as: 1;

The sequence of this isoform differs from the canonical sequence as follows:
     72-88: RLPKSASLVDSLVSEAT → S
     197-204: GKGDEVKI → V
     370-374: NEVAT → LAFNY
     375-551: Missing.
Isoform G (identifier: Q960T2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     72-88: RLPKSASLVDSLVSEAT → S
Note: No experimental confirmation available.
Isoform I (identifier: Q960T2-3)

Also known as: 2;

The sequence of this isoform differs from the canonical sequence as follows:
     72-88: RLPKSASLVDSLVSEAT → S
     197-204: GKGDEVKI → V
     369-370: AN → ED
     371-551: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Synapse-associated protein of 47 kDa
PRO_0000097584

Regions

Domain295 – 34753BSD

Amino acid modifications

Modified residue1781Phosphoserine Ref.5
Modified residue1821Phosphoserine Ref.5
Modified residue1861Phosphothreonine Ref.5
Modified residue4331Phosphoserine Ref.5
Modified residue5301Phosphothreonine Ref.5

Natural variations

Alternative sequence72 – 8817RLPKS…VSEAT → S in isoform F, isoform G and isoform I.
VSP_007389
Alternative sequence197 – 2048GKGDEVKI → V in isoform F and isoform I.
VSP_007390
Alternative sequence369 – 3702AN → ED in isoform I.
VSP_007391
Alternative sequence370 – 3745NEVAT → LAFNY in isoform B and isoform F.
VSP_007393
Alternative sequence371 – 551181Missing in isoform I.
VSP_007392
Alternative sequence375 – 551177Missing in isoform B and isoform F.
VSP_007394

Sequences

Sequence LengthMass (Da)Tools
Isoform A (C) (D) (E) (H) [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 430392D0EAD5916E

FASTA55156,980
        10         20         30         40         50         60 
MFSGLTNQFT SLVGAVKGGA GDEDVPAPTG DAPAAAPAAS TSVEATASSA VDPEAAAAAG 

        70         80         90        100        110        120 
GEGLEGEEAG KRLPKSASLV DSLVSEATGW LGSAKGWLGN ASIPSMPAMP SMPSMPAMPA 

       130        140        150        160        170        180 
MPSIPSIPGL RKGAGADGAE GAEGAVAGEG GAAASGAVSG GEDDDKSRYI SATEGADSHP 

       190        200        210        220        230        240 
ASGGGTPTGD EGQIGQGKGD EVKITTKVTQ QAKHFGSFLS SAISKAGSKI KETVKDNTIL 

       250        260        270        280        290        300 
DSFNKEQEAF IKGQGGVGNG AAPWIGHANE AKIKEEILGL SQDRRNFVRA PPAGVDFEFS 

       310        320        330        340        350        360 
YDTAYPTAIA IMAEDKALET MRFELVPKII TEENFWRNYF YRVSLIIQAA ELGTLGADGV 

       370        380        390        400        410        420 
GQASSGEDAN EVATKEKKSK TAEPAKGDSS VKAIAEQPKA VIEPEAQECD VQAAKSKAKA 

       430        440        450        460        470        480 
KAQAGKELGQ KISESEFVSD DFQASSESDL AEIQDGMRKL GIDSMTQQAL AATDEEQWEK 

       490        500        510        520        530        540 
DLEAELKDYE VVDEGGTGGD GGGGRRKGRK AGEDDTEADE DEPTISNLRT RSTNNDWEEY 

       550 
ADLIEDTDDL K 

« Hide

Isoform B [UniParc].

Checksum: 42AF0887B9A2961F
Show »

FASTA37437,891
Isoform F (1) [UniParc].

Checksum: 5D82A5B308551B40
Show »

FASTA35135,495
Isoform G [UniParc].

Checksum: DC70FD0FCF9CF667
Show »

FASTA53555,313
Isoform I (2) [UniParc].

Checksum: A5D3DB403CFBCE67
Show »

FASTA34735,060

References

« Hide 'large scale' references
[1]"The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals."
Reichmuth C., Becker S., Benz M., Debel K., Reisch D., Heimbeck G., Hofbauer A., Klagges B., Pflugfelder G.O., Buchner E.
Brain Res. Mol. Brain Res. 32:45-54(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS F AND I), TISSUE SPECIFICITY.
Strain: Berlin.
Tissue: Head.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178; SER-182; THR-186; SER-433 AND THR-530, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80110 mRNA. Translation: CAA56415.1.
X80111 mRNA. Translation: CAA56416.1.
AE014297 Genomic DNA. Translation: AAF55255.2.
AE014297 Genomic DNA. Translation: AAN13682.1.
AE014297 Genomic DNA. Translation: AAN13683.1.
AE014297 Genomic DNA. Translation: AAN13684.1.
AE014297 Genomic DNA. Translation: AAN13685.1.
AE014297 Genomic DNA. Translation: AAN13686.2.
AE014297 Genomic DNA. Translation: AAN13687.1.
AE014297 Genomic DNA. Translation: AAN13688.1.
AY051871 mRNA. Translation: AAK93295.1.
PIRS60653.
RefSeqNP_524371.1. NM_079647.4.
NP_732088.1. NM_169684.3.
NP_732089.1. NM_169685.3.
NP_732090.1. NM_169686.3.
NP_732091.1. NM_169687.3.
NP_732092.2. NM_169688.3.
NP_732093.1. NM_169689.3.
NP_732094.1. NM_169690.3.
UniGeneDm.4457.

3D structure databases

ProteinModelPortalQ960T2.
SMRQ960T2. Positions 260-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid66992. 13 interactions.
DIPDIP-18495N.
IntActQ960T2. 1 interaction.
STRING7227.FBpp0082661.

Proteomic databases

PaxDbQ960T2.
PRIDEQ960T2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0083204; FBpp0082658; FBgn0013334. [Q960T2-1]
FBtr0083206; FBpp0082660; FBgn0013334. [Q960T2-1]
FBtr0083207; FBpp0082661; FBgn0013334. [Q960T2-1]
FBtr0083208; FBpp0082662; FBgn0013334. [Q960T2-1]
GeneID41938.
KEGGdme:Dmel_CG8884.

Organism-specific databases

CTD41938.
FlyBaseFBgn0013334. Sap47.

Phylogenomic databases

eggNOGNOG283411.
GeneTreeENSGT00390000007662.
InParanoidQ960T2.
OMANASIPSM.
OrthoDBEOG7WDN49.
PhylomeDBQ960T2.

Gene expression databases

BgeeQ960T2.

Family and domain databases

InterProIPR005607. BSD.
[Graphical view]
PfamPF03909. BSD. 1 hit.
[Graphical view]
SMARTSM00751. BSD. 1 hit.
[Graphical view]
PROSITEPS50858. BSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSap47. drosophila.
GenomeRNAi41938.
NextBio826353.
PROQ960T2.

Entry information

Entry nameSAP47_DROME
AccessionPrimary (citable) accession number: Q960T2
Secondary accession number(s): A4V2Z5 expand/collapse secondary AC list , Q24502, Q24503, Q8INC9, Q9VF07
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase