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Q960T2

- SAP47_DROME

UniProt

Q960T2 - SAP47_DROME

Protein

Synapse-associated protein of 47 kDa

Gene

Sap47

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    GO - Biological processi

    1. associative learning Source: FlyBase
    2. regulation of short-term neuronal synaptic plasticity Source: FlyBase
    3. synaptic transmission Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synapse-associated protein of 47 kDa
    Gene namesi
    Name:Sap47
    ORF Names:CG8884
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0013334. Sap47.

    Subcellular locationi

    GO - Cellular componenti

    1. synapse Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Synapse-associated protein of 47 kDaPRO_0000097584Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei178 – 1781Phosphoserine1 Publication
    Modified residuei182 – 1821Phosphoserine1 Publication
    Modified residuei186 – 1861Phosphothreonine1 Publication
    Modified residuei433 – 4331Phosphoserine1 Publication
    Modified residuei530 – 5301Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ960T2.
    PRIDEiQ960T2.

    Expressioni

    Tissue specificityi

    Expressed specifically in neurons and transported to synaptic terminals.1 Publication

    Gene expression databases

    BgeeiQ960T2.

    Interactioni

    Protein-protein interaction databases

    BioGridi66992. 13 interactions.
    DIPiDIP-18495N.
    IntActiQ960T2. 1 interaction.
    STRINGi7227.FBpp0082661.

    Structurei

    3D structure databases

    ProteinModelPortaliQ960T2.
    SMRiQ960T2. Positions 260-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini295 – 34753BSDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BSD domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG283411.
    GeneTreeiENSGT00390000007662.
    InParanoidiQ960T2.
    OMAiNASIPSM.
    OrthoDBiEOG7WDN49.
    PhylomeDBiQ960T2.

    Family and domain databases

    InterProiIPR005607. BSD.
    [Graphical view]
    PfamiPF03909. BSD. 1 hit.
    [Graphical view]
    SMARTiSM00751. BSD. 1 hit.
    [Graphical view]
    PROSITEiPS50858. BSD. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q960T2-1) [UniParc]FASTAAdd to Basket

    Also known as: C, D, E, H

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFSGLTNQFT SLVGAVKGGA GDEDVPAPTG DAPAAAPAAS TSVEATASSA    50
    VDPEAAAAAG GEGLEGEEAG KRLPKSASLV DSLVSEATGW LGSAKGWLGN 100
    ASIPSMPAMP SMPSMPAMPA MPSIPSIPGL RKGAGADGAE GAEGAVAGEG 150
    GAAASGAVSG GEDDDKSRYI SATEGADSHP ASGGGTPTGD EGQIGQGKGD 200
    EVKITTKVTQ QAKHFGSFLS SAISKAGSKI KETVKDNTIL DSFNKEQEAF 250
    IKGQGGVGNG AAPWIGHANE AKIKEEILGL SQDRRNFVRA PPAGVDFEFS 300
    YDTAYPTAIA IMAEDKALET MRFELVPKII TEENFWRNYF YRVSLIIQAA 350
    ELGTLGADGV GQASSGEDAN EVATKEKKSK TAEPAKGDSS VKAIAEQPKA 400
    VIEPEAQECD VQAAKSKAKA KAQAGKELGQ KISESEFVSD DFQASSESDL 450
    AEIQDGMRKL GIDSMTQQAL AATDEEQWEK DLEAELKDYE VVDEGGTGGD 500
    GGGGRRKGRK AGEDDTEADE DEPTISNLRT RSTNNDWEEY ADLIEDTDDL 550
    K 551

    Note: No experimental confirmation available.

    Length:551
    Mass (Da):56,980
    Last modified:December 1, 2001 - v1
    Checksum:i430392D0EAD5916E
    GO
    Isoform B (identifier: Q960T2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         370-374: NEVAT → LAFNY
         375-551: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:374
    Mass (Da):37,891
    Checksum:i42AF0887B9A2961F
    GO
    Isoform F (identifier: Q960T2-4) [UniParc]FASTAAdd to Basket

    Also known as: 1

    The sequence of this isoform differs from the canonical sequence as follows:
         72-88: RLPKSASLVDSLVSEAT → S
         197-204: GKGDEVKI → V
         370-374: NEVAT → LAFNY
         375-551: Missing.

    Show »
    Length:351
    Mass (Da):35,495
    Checksum:i5D82A5B308551B40
    GO
    Isoform G (identifier: Q960T2-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         72-88: RLPKSASLVDSLVSEAT → S

    Note: No experimental confirmation available.

    Show »
    Length:535
    Mass (Da):55,313
    Checksum:iDC70FD0FCF9CF667
    GO
    Isoform I (identifier: Q960T2-3) [UniParc]FASTAAdd to Basket

    Also known as: 2

    The sequence of this isoform differs from the canonical sequence as follows:
         72-88: RLPKSASLVDSLVSEAT → S
         197-204: GKGDEVKI → V
         369-370: AN → ED
         371-551: Missing.

    Show »
    Length:347
    Mass (Da):35,060
    Checksum:iA5D3DB403CFBCE67
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei72 – 8817RLPKS…VSEAT → S in isoform F, isoform G and isoform I. 1 PublicationVSP_007389Add
    BLAST
    Alternative sequencei197 – 2048GKGDEVKI → V in isoform F and isoform I. 1 PublicationVSP_007390
    Alternative sequencei369 – 3702AN → ED in isoform I. 1 PublicationVSP_007391
    Alternative sequencei370 – 3745NEVAT → LAFNY in isoform B and isoform F. 1 PublicationVSP_007393
    Alternative sequencei371 – 551181Missing in isoform I. 1 PublicationVSP_007392Add
    BLAST
    Alternative sequencei375 – 551177Missing in isoform B and isoform F. 1 PublicationVSP_007394Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80110 mRNA. Translation: CAA56415.1.
    X80111 mRNA. Translation: CAA56416.1.
    AE014297 Genomic DNA. Translation: AAF55255.2.
    AE014297 Genomic DNA. Translation: AAN13682.1.
    AE014297 Genomic DNA. Translation: AAN13683.1.
    AE014297 Genomic DNA. Translation: AAN13684.1.
    AE014297 Genomic DNA. Translation: AAN13685.1.
    AE014297 Genomic DNA. Translation: AAN13686.2.
    AE014297 Genomic DNA. Translation: AAN13687.1.
    AE014297 Genomic DNA. Translation: AAN13688.1.
    AY051871 mRNA. Translation: AAK93295.1.
    PIRiS60653.
    RefSeqiNP_524371.1. NM_079647.4. [Q960T2-4]
    NP_732088.1. NM_169684.3. [Q960T2-1]
    NP_732089.1. NM_169685.3. [Q960T2-1]
    NP_732090.1. NM_169686.3. [Q960T2-1]
    NP_732091.1. NM_169687.3. [Q960T2-1]
    NP_732092.2. NM_169688.3. [Q960T2-3]
    NP_732093.1. NM_169689.3. [Q960T2-5]
    NP_732094.1. NM_169690.3. [Q960T2-2]
    UniGeneiDm.4457.

    Genome annotation databases

    EnsemblMetazoaiFBtr0083204; FBpp0082658; FBgn0013334. [Q960T2-1]
    FBtr0083206; FBpp0082660; FBgn0013334. [Q960T2-1]
    FBtr0083207; FBpp0082661; FBgn0013334. [Q960T2-1]
    FBtr0083208; FBpp0082662; FBgn0013334. [Q960T2-1]
    GeneIDi41938.
    KEGGidme:Dmel_CG8884.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80110 mRNA. Translation: CAA56415.1 .
    X80111 mRNA. Translation: CAA56416.1 .
    AE014297 Genomic DNA. Translation: AAF55255.2 .
    AE014297 Genomic DNA. Translation: AAN13682.1 .
    AE014297 Genomic DNA. Translation: AAN13683.1 .
    AE014297 Genomic DNA. Translation: AAN13684.1 .
    AE014297 Genomic DNA. Translation: AAN13685.1 .
    AE014297 Genomic DNA. Translation: AAN13686.2 .
    AE014297 Genomic DNA. Translation: AAN13687.1 .
    AE014297 Genomic DNA. Translation: AAN13688.1 .
    AY051871 mRNA. Translation: AAK93295.1 .
    PIRi S60653.
    RefSeqi NP_524371.1. NM_079647.4. [Q960T2-4 ]
    NP_732088.1. NM_169684.3. [Q960T2-1 ]
    NP_732089.1. NM_169685.3. [Q960T2-1 ]
    NP_732090.1. NM_169686.3. [Q960T2-1 ]
    NP_732091.1. NM_169687.3. [Q960T2-1 ]
    NP_732092.2. NM_169688.3. [Q960T2-3 ]
    NP_732093.1. NM_169689.3. [Q960T2-5 ]
    NP_732094.1. NM_169690.3. [Q960T2-2 ]
    UniGenei Dm.4457.

    3D structure databases

    ProteinModelPortali Q960T2.
    SMRi Q960T2. Positions 260-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 66992. 13 interactions.
    DIPi DIP-18495N.
    IntActi Q960T2. 1 interaction.
    STRINGi 7227.FBpp0082661.

    Proteomic databases

    PaxDbi Q960T2.
    PRIDEi Q960T2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0083204 ; FBpp0082658 ; FBgn0013334 . [Q960T2-1 ]
    FBtr0083206 ; FBpp0082660 ; FBgn0013334 . [Q960T2-1 ]
    FBtr0083207 ; FBpp0082661 ; FBgn0013334 . [Q960T2-1 ]
    FBtr0083208 ; FBpp0082662 ; FBgn0013334 . [Q960T2-1 ]
    GeneIDi 41938.
    KEGGi dme:Dmel_CG8884.

    Organism-specific databases

    CTDi 41938.
    FlyBasei FBgn0013334. Sap47.

    Phylogenomic databases

    eggNOGi NOG283411.
    GeneTreei ENSGT00390000007662.
    InParanoidi Q960T2.
    OMAi NASIPSM.
    OrthoDBi EOG7WDN49.
    PhylomeDBi Q960T2.

    Miscellaneous databases

    ChiTaRSi Sap47. drosophila.
    GenomeRNAii 41938.
    NextBioi 826353.
    PROi Q960T2.

    Gene expression databases

    Bgeei Q960T2.

    Family and domain databases

    InterProi IPR005607. BSD.
    [Graphical view ]
    Pfami PF03909. BSD. 1 hit.
    [Graphical view ]
    SMARTi SM00751. BSD. 1 hit.
    [Graphical view ]
    PROSITEi PS50858. BSD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals."
      Reichmuth C., Becker S., Benz M., Debel K., Reisch D., Heimbeck G., Hofbauer A., Klagges B., Pflugfelder G.O., Buchner E.
      Brain Res. Mol. Brain Res. 32:45-54(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS F AND I), TISSUE SPECIFICITY.
      Strain: Berlin.
      Tissue: Head.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Embryo.
    5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178; SER-182; THR-186; SER-433 AND THR-530, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSAP47_DROME
    AccessioniPrimary (citable) accession number: Q960T2
    Secondary accession number(s): A4V2Z5
    , Q24502, Q24503, Q8INC9, Q9VF07
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3