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Protein

Protein cortex

Gene

cort

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Female meiosis-specific activator of the anaphase promoting complex/cyclosome (APC/C) (PubMed:17251266, PubMed:18020708). Required for the completion of meiosis in oocytes (PubMed:11252055, PubMed:10683177, PubMed:10924478, PubMed:17251266, PubMed:18020708). Activates the ubiquitin ligase activity and substrate specificity of APC/C and triggers the sequential degradation of mitotic cyclins in meiosis (PubMed:17251266, PubMed:18020708). Promotes the ubiquitination and degradation of CycA early in meiosis I and the degradation of CycB and CycB3 after egg activation (PubMed:18020708). Promotes degradation of mtrm at the oocyte-to-embryo transition (PubMed:24019759).6 Publications

GO - Molecular functioni

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: FlyBase
  • cellularization Source: FlyBase
  • egg activation Source: FlyBase
  • female meiosis I Source: FlyBase
  • female meiosis II Source: FlyBase
  • female meiotic nuclear division Source: UniProtKB
  • homologous chromosome segregation Source: FlyBase
  • oogenesis Source: UniProtKB-KW
  • pole cell formation Source: FlyBase
  • positive regulation of ubiquitin-protein ligase activity involved in meiotic cell cycle Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Meiosis, Oogenesis

Enzyme and pathway databases

SignaLinkiQ960N3.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein cortex1 Publication
Gene namesi
Name:cort1 Publication
ORF Names:CG11330
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000351. cort.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi303Y → C in QW55; abnormal arrest in female meiosis. 1 Publication1
Mutagenesisi424 – 432RGTLFLMWN → AGTAFLMWA: Prevents degradation following egg activation. 1 Publication9
Mutagenesisi431 – 483Missing in RH65; abnormal arrest in female meiosis. 1 PublicationAdd BLAST53

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509411 – 483Protein cortexAdd BLAST483

Post-translational modificationi

Probably ubiquitinated: degraded following egg activation by the anaphase promoting complex/cyclosome (APC/C).1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ960N3.
PRIDEiQ960N3.

Expressioni

Tissue specificityi

Highly expressed in ovaries. Expressed in nurse cells from stage 6 to stage 12 egg chambers. As oocyte matures, it is transferred from the nurse cells to the oocyte.1 Publication

Developmental stagei

Undetectable in early stage 1-8 egg chambers and present at very low levels in stages 9-10B egg chambers. Strongly increases in stage 12-13 egg chambers and remains high in mature stage 14 oocytes (PubMed:18020708). Protein levels drop dramatically by the time meiosis is completed in unfertilized eggs (at protein level) (PubMed:18020708).1 Publication

Inductioni

Transcription is activated by grauzone (grau), which binds to its promoter region.2 Publications

Gene expression databases

BgeeiFBgn0000351.
ExpressionAtlasiQ960N3. differential.
GenevisibleiQ960N3. DM.

Interactioni

Subunit structurei

Interacts with anaphase promoting complex/cyclosome (APC/C) subunits Cdc16 and Cdc27 (PubMed:18020708). Interacts with mtrm (PubMed:24019759).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mtrmQ239733EBI-3423056,EBI-166563

GO - Molecular functioni

  • anaphase-promoting complex binding Source: FlyBase

Protein-protein interaction databases

BioGridi60090. 9 interactors.
DIPiDIP-60666N.
ELMiQ960N3.
IntActiQ960N3. 8 interactors.
STRINGi7227.FBpp0078949.

Structurei

3D structure databases

ProteinModelPortaliQ960N3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati158 – 195WD 1Add BLAST38
Repeati196 – 237WD 2Add BLAST42
Repeati245 – 284WD 3Add BLAST40
Repeati287 – 326WD 4Add BLAST40
Repeati421 – 460WD 5Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi424 – 432D-box1 Publication9

Sequence similaritiesi

Belongs to the WD repeat CORT family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IMNW. Eukaryota.
ENOG4112CYB. LUCA.
InParanoidiQ960N3.
OMAiSYWRQSG.
OrthoDBiEOG091G0A9L.
PhylomeDBiQ960N3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PANTHERiPTHR19918. PTHR19918. 1 hit.
PfamiView protein in Pfam
PF00400. WD40. 2 hits.
SMARTiView protein in SMART
SM00320. WD40. 4 hits.
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiView protein in PROSITE
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Q960N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVEMSVCDS PNKNSKLDKK SLTPFKKVRR KNWKQEAAYK SDTSKGQEVS
60 70 80 90 100
YVGERFIPNR FERENIEFNL KYIGKRKERD ILETGVTLTA SYWRQSGFIS
110 120 130 140 150
NINRTFGIGE RRLFQFSSQQ GTRSRVVDND SADSDWPCNP RARPYAIQNA
160 170 180 190 200
THEMPGICSP VDYNMMDWSS GGMVAMSSGQ DVMLWRNLDE STMVFSVESP
210 220 230 240 250
TSLKYSPDGK HLAIGCMDRN YPVLDLWEVR SPTEFLVSYR KLFFKSMGYI
260 270 280 290 300
SCIEWSHDGK EVICGTQCGV IIVLAMPTLN TLMQLREHRH TVKKMKFAPT
310 320 330 340 350
HKYFASSDTD GKIFIFDAVL KVRLLKLDGR SIVFDWHPWT GEDLAVAERS
360 370 380 390 400
PASIFIFNIP RRQFVASYRR RDDRIVIKTL TYSKITGELL VNVIRRDDAD
410 420 430 440 450
LAVCEILVLA SLNRVVDLMS HQDRGTLFLM WNPDGTKIAT GGLDDTFSLW
460 470 480
NFFPTYKREA ILRKQEQKAK DKCSSLSLYK GIR
Length:483
Mass (Da):55,575
Last modified:April 23, 2003 - v2
Checksum:i007DD0190DE06735
GO

Sequence cautioni

The sequence AAK93390 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033478 mRNA. Translation: AAK54464.1.
AE014134 Genomic DNA. Translation: AAF52421.1.
AY051966 mRNA. Translation: AAK93390.1. Different initiation.
RefSeqiNP_001260144.1. NM_001273215.1.
NP_523494.1. NM_078770.2.
UniGeneiDm.12510.

Genome annotation databases

EnsemblMetazoaiFBtr0079320; FBpp0078949; FBgn0000351.
FBtr0333350; FBpp0305542; FBgn0000351.
GeneIDi33937.
KEGGidme:Dmel_CG11330.
UCSCiCG11330-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiCORT_DROME
AccessioniPrimary (citable) accession number: Q960N3
Secondary accession number(s): Q9VMA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: November 22, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families