Q95ZQ4 (AAPK2_CAEEL) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 83.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 5'-AMP-activated protein kinase catalytic subunit alpha-2 Short name=AMPK subunit alpha-2 EC=2.7.11.1 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 626 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals. Role in motility and response to oxidative stress. Causes germ cell cycle arrest upon dauer entry. Ref.1 Ref.6 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Activated by phosphorylation. Ref.1 |
| Subunit structure | Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer that binds four cAMP molecules. |
| Tissue specificity | Expressed in the pharynx, the ventral cord, neurons including the hermaphrodite-specific neuron, body wall muscles, the vulva, the excretory canal, and weakly in the intestine. Ref.6 |
| Post-translational modification | Phosphorylated on Thr-243 in response to oxidative stress. Ref.1 |
| Disruption phenotype | Shortened life-span. Temperature-dependent hypersensitivity to stress, slow body bending, abnormal modulation of head oscillation. Ref.1 Ref.6 |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| aakb-2 | Q9NAH7 | 4 | EBI-326514,EBI-2417663 |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform b Ref.4 (identifier: Q95ZQ4-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform a Ref.1 (identifier: Q95ZQ4-2) The sequence of this isoform differs from the canonical sequence as follows: 370-371: Missing. | ||||||
| Isoform c Ref.4 (identifier: Q95ZQ4-3) The sequence of this isoform differs from the canonical sequence as follows: 1-62: Missing. 370-371: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 626 | 626 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | PRO_0000085599 | |||||
Regions | |||||||||
| Domain | 87 – 339 | 253 | Protein kinase | ||||||
| Nucleotide binding | 93 – 101 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 210 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 116 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 243 | 1 | Phosphothreonine; by par-4 Ref.1 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 62 | 62 | Missing in isoform c. Ref.4 | VSP_051940 | |||||
| Alternative sequence | 370 – 371 | 2 | Missing in isoform a and isoform c. Ref.1 Ref.4 | VSP_051941 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans." Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R. Genes Dev. 18:3004-3009(2004) [PubMed: 15574588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, ENZYME REGULATION, DISRUPTION PHENOTYPE, PHOSPHORYLATION AT THR-243. |
| [2] | Erratum Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R. Genes Dev. 19:188-188(2005) |
| [3] | Erratum Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R. Genes Dev. 19:411-411(2005) |
| [4] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| [5] | "Cloning, structure, and expression of the gene for a novel regulatory subunit of cAMP-dependent protein kinase in Caenorhabditis elegans." Lu X., Gross R.E., Bagchi S., Rubin C.S. J. Biol. Chem. 265:3293-3303(1990) [PubMed: 2303451] [Abstract] Cited for: IDENTIFICATION IN AMPK COMPLEX. |
| [6] | "The Caenorhabditis elegans AMP-activated protein kinase AAK-2 is phosphorylated by LKB1 and is required for resistance to oxidative stress and for normal motility and foraging behavior." Lee H., Cho J.S., Lambacher N., Lee J., Lee S.J., Lee T.H., Gartner A., Koo H.S. J. Biol. Chem. 283:14988-14993(2008) [PubMed: 18408008] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY347273 mRNA. Translation: AAR06928.1. FO081053 Genomic DNA. Translation: CCD68833.1. FO081053 Genomic DNA. Translation: CCD68834.1. FO081053 Genomic DNA. Translation: CCD68835.1. |
| PIR | T29858. |
| RefSeq | NP_001024868.1. NM_001029697.2. NP_510710.2. NM_078309.3. NP_510711.2. NM_078310.4. |
| UniGene | Cel.17479. |
3D structure databases | |
| ProteinModelPortal | Q95ZQ4. |
| SMR | Q95ZQ4. Positions 80-626. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-25170N. |
| IntAct | Q95ZQ4. 7 interactions. |
| MINT | MINT-1049339. |
| STRING | Q95ZQ4. |
Proteomic databases | |
| PRIDE | Q95ZQ4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | T01C8.1b; T01C8.1b; T01C8.1. |
| GeneID | 181727. |
| KEGG | cel:T01C8.1. |
| UCSC | T01C8.1c.1. c. elegans. |
Organism-specific databases | |
| CTD | 181727. |
| WormBase | T01C8.1a; CE31222; WBGene00020142; aak-2. T01C8.1b; CE31223; WBGene00020142; aak-2. T01C8.1c; CE07458; WBGene00020142; aak-2. |
Phylogenomic databases | |
| eggNOG | meNOG05149. |
| GeneTree | EMGT00050000001128. |
| HOGENOM | HBG755340. |
| InParanoid | Q95ZQ4. |
| OMA | YICKNGK. |
| PhylomeDB | Q95ZQ4. |
Gene expression databases | |
| ArrayExpress | Q95ZQ4. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view] |
| KO | K07198. |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 915106. |
Entry information
| Entry name | AAPK2_CAEEL | ||||||||
| Accession | Primary (citable) accession number: Q95ZQ4 Secondary accession number(s): Q22068, Q86FL6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| SIMILARITY comments Index of protein domains and families |

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