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Protein

5'-AMP-activated protein kinase catalytic subunit alpha-2

Gene

aak-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals (PubMed:15574588). Role in motility and response to oxidative stress (PubMed:18408008, PubMed:24763318). Involved in the establishment of germline stem cell (GSC) quiescence during dauer development (PubMed:15574588, PubMed:20110331). Plays a role in axon regrowth after axotomy in PLM neurons (PubMed:24431434). Plays a role in the maintenance of glycogen stores which are necessary for resistance to hyperosmotic stress (PubMed:26439621).6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161ATPPROSITE-ProRule annotation
Active sitei210 – 2101Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 1019ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • AMP-activated protein kinase activity Source: WormBase
  • ATP binding Source: UniProtKB-KW
  • histone serine kinase activity Source: GO_Central

GO - Biological processi

  • cellular response to glucose starvation Source: GO_Central
  • determination of adult lifespan Source: WormBase
  • intracellular signal transduction Source: GO_Central
  • negative regulation of TOR signaling Source: GO_Central
  • peptidyl-serine phosphorylation Source: WormBase
  • positive regulation of dauer larval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1632852. Macroautophagy.
R-CEL-163680. AMPK inhibits chREBP transcriptional activation activity.
R-CEL-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-CEL-5628897. TP53 Regulates Metabolic Genes.
SignaLinkiQ95ZQ4.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-AMP-activated protein kinase catalytic subunit alpha-2 (EC:2.7.11.1)
Short name:
AMPK subunit alpha-2
Gene namesi
Name:aak-2
ORF Names:T01C8.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiT01C8.1a; CE31222; WBGene00020142; aak-2.
T01C8.1b; CE31223; WBGene00020142; aak-2.
T01C8.1c; CE07458; WBGene00020142; aak-2.

Subcellular locationi

GO - Cellular componenti

  • axon Source: WormBase
  • cytoplasm Source: WormBase
  • neuronal cell body Source: WormBase
  • nucleotide-activated protein kinase complex Source: GO_Central
  • nucleus Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Shortened life-span (PubMed:15574588, PubMed:18408008). Temperature-dependent hypersensitivity to stress, slow body bending, abnormal modulation of head oscillation (PubMed:15574588, PubMed:18408008). Reduced survival following oxidative stress induced by the superoxide paraquat (PubMed:24763318). Reduced survival as a result of hyperosmotic stress induced by NaCl (PubMed:26439621). Double knockout with aak-1 results in reduced glycogen accumulation (PubMed:26439621).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6266265'-AMP-activated protein kinase catalytic subunit alpha-2PRO_0000085599Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei243 – 2431Phosphothreonine; by par-42 Publications

Post-translational modificationi

Phosphorylated on Thr-243 in response to oxidative stress and during dauer development.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ95ZQ4.
PaxDbiQ95ZQ4.
PRIDEiQ95ZQ4.

PTM databases

iPTMnetiQ95ZQ4.

Expressioni

Tissue specificityi

Expressed in the pharynx, the ventral cord, neurons including the hermaphrodite-specific neuron, body wall muscles, the vulva, the excretory canal, and weakly in the intestine.1 Publication

Gene expression databases

BgeeiWBGene00020142.

Interactioni

Subunit structurei

Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer that binds four cAMP molecules.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
aakb-2Q9NAH74EBI-326514,EBI-2417663

Protein-protein interaction databases

BioGridi46610. 8 interactions.
DIPiDIP-25170N.
IntActiQ95ZQ4. 7 interactions.
MINTiMINT-1049339.
STRINGi6239.T01C8.1b.

Structurei

3D structure databases

ProteinModelPortaliQ95ZQ4.
SMRiQ95ZQ4. Positions 20-538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 339253Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0586. Eukaryota.
ENOG410XNQ0. LUCA.
GeneTreeiENSGT00790000122947.
HOGENOMiHOG000233016.
InParanoidiQ95ZQ4.
KOiK07198.
OMAiNYRSCQK.
OrthoDBiEOG091G03TG.
PhylomeDBiQ95ZQ4.

Family and domain databases

InterProiIPR032270. AMPK_C.
IPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF16579. AdenylateSensor. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b1 Publication (identifier: Q95ZQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSHQDRDRD RKEDGGGDGT EMKSKSRSQP SGLNRVKNLS RKLSAKSRKE
60 70 80 90 100
RKDRDSTDNS SKMSSPGGET STKQQQELKA QIKIGHYILK ETLGVGTFGK
110 120 130 140 150
VKVGIHETTQ YKVAVKILNR QKIKSLDVVG KIRREIQNLS LFRHPHIIRL
160 170 180 190 200
YQVISTPSDI FMIMEHVSGG ELFDYIVKHG RLKTAEARRF FQQIISGVDY
210 220 230 240 250
CHRHMVVHRD LKPENLLLDE QNNVKIADFG LSNIMTDGDF LRTSCGSPNY
260 270 280 290 300
AAPEVISGKL YAGPEVDVWS CGVILYALLC GTLPFDDEHV PSLFRKIKSG
310 320 330 340 350
VFPTPDFLER PIVNLLHHML CVDPMKRATI KDVIAHEWFQ KDLPNYLFPP
360 370 380 390 400
INESEASIVD IEAVREVTEF QRYHVAEEEV TSALLGDDPH HHLSIAYNLI
410 420 430 440 450
VDNKRIADET AKLSIEEFYQ VTPNKGPGPV HRHPERIAAS VSSKITPTLD
460 470 480 490 500
NTEASGANRN KRAKWHLGIR SQSRPEDIMF EVFRAMKQLD MEWKVLNPYH
510 520 530 540 550
VIVRRKPDAP AADPPKMSLQ LYQVDQRSYL LDFKSLADEE SGSASASSSR
560 570 580 590 600
HASMSMPQKP AGIRGTRTSS MPQAMSMEAS IEKMEVHDFS DMSCDVTPPP
610 620
SPGGAKLSQT MQFFEICAAL IGTLAR
Note: No experimental confirmation available.Curated
Length:626
Mass (Da):70,443
Last modified:October 1, 2002 - v2
Checksum:iD1401DB1286D8624
GO
Isoform a1 Publication (identifier: Q95ZQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: Missing.

Show »
Length:624
Mass (Da):70,168
Checksum:i3BF9F9610BA95DB7
GO
Isoform c1 Publication (identifier: Q95ZQ4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.
     370-371: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:562
Mass (Da):63,103
Checksum:iAA34A96C5BBCAFBD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6262Missing in isoform c. 1 PublicationVSP_051940Add
BLAST
Alternative sequencei370 – 3712Missing in isoform a and isoform c. 2 PublicationsVSP_051941

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY347273 mRNA. Translation: AAR06928.1.
FO081053 Genomic DNA. Translation: CCD68833.1.
FO081053 Genomic DNA. Translation: CCD68834.1.
FO081053 Genomic DNA. Translation: CCD68835.1.
PIRiT29858.
RefSeqiNP_001024868.1. NM_001029697.3. [Q95ZQ4-3]
NP_510710.2. NM_078309.4. [Q95ZQ4-1]
NP_510711.2. NM_078310.5. [Q95ZQ4-2]
UniGeneiCel.17479.

Genome annotation databases

EnsemblMetazoaiT01C8.1b; T01C8.1b; WBGene00020142. [Q95ZQ4-1]
GeneIDi181727.
KEGGicel:CELE_T01C8.1.
UCSCiT01C8.1c.1. c. elegans. [Q95ZQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY347273 mRNA. Translation: AAR06928.1.
FO081053 Genomic DNA. Translation: CCD68833.1.
FO081053 Genomic DNA. Translation: CCD68834.1.
FO081053 Genomic DNA. Translation: CCD68835.1.
PIRiT29858.
RefSeqiNP_001024868.1. NM_001029697.3. [Q95ZQ4-3]
NP_510710.2. NM_078309.4. [Q95ZQ4-1]
NP_510711.2. NM_078310.5. [Q95ZQ4-2]
UniGeneiCel.17479.

3D structure databases

ProteinModelPortaliQ95ZQ4.
SMRiQ95ZQ4. Positions 20-538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi46610. 8 interactions.
DIPiDIP-25170N.
IntActiQ95ZQ4. 7 interactions.
MINTiMINT-1049339.
STRINGi6239.T01C8.1b.

PTM databases

iPTMnetiQ95ZQ4.

Proteomic databases

EPDiQ95ZQ4.
PaxDbiQ95ZQ4.
PRIDEiQ95ZQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT01C8.1b; T01C8.1b; WBGene00020142. [Q95ZQ4-1]
GeneIDi181727.
KEGGicel:CELE_T01C8.1.
UCSCiT01C8.1c.1. c. elegans. [Q95ZQ4-1]

Organism-specific databases

CTDi181727.
WormBaseiT01C8.1a; CE31222; WBGene00020142; aak-2.
T01C8.1b; CE31223; WBGene00020142; aak-2.
T01C8.1c; CE07458; WBGene00020142; aak-2.

Phylogenomic databases

eggNOGiKOG0586. Eukaryota.
ENOG410XNQ0. LUCA.
GeneTreeiENSGT00790000122947.
HOGENOMiHOG000233016.
InParanoidiQ95ZQ4.
KOiK07198.
OMAiNYRSCQK.
OrthoDBiEOG091G03TG.
PhylomeDBiQ95ZQ4.

Enzyme and pathway databases

ReactomeiR-CEL-1632852. Macroautophagy.
R-CEL-163680. AMPK inhibits chREBP transcriptional activation activity.
R-CEL-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-CEL-5628897. TP53 Regulates Metabolic Genes.
SignaLinkiQ95ZQ4.

Miscellaneous databases

PROiQ95ZQ4.

Gene expression databases

BgeeiWBGene00020142.

Family and domain databases

InterProiIPR032270. AMPK_C.
IPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF16579. AdenylateSensor. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAPK2_CAEEL
AccessioniPrimary (citable) accession number: Q95ZQ4
Secondary accession number(s): Q22068, Q86FL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.