Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot Q95ZQ4 (AAPK2_CAEEL)

Last modified June 16, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    5'-AMP-activated protein kinase catalytic subunit alpha-2
      Short name=AMP-activated kinase 2
    EC=2.7.11.1
Gene names
Name: aak-2
ORF Names: T01C8.1
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by phosphorylation. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform b Ref.4 (identifier: Q95ZQ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform a Ref.1 (identifier: Q95ZQ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: Missing.
Isoform c Ref.4 (identifier: Q95ZQ4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.
     370-371: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6266265'-AMP-activated protein kinase catalytic subunit alpha-2
PRO_0000085599

Regions

Domain87 – 339253Protein kinase
Nucleotide binding93 – 1019ATP By similarity

Sites

Active site2101Proton acceptor By similarity
Binding site1161ATP By similarity

Natural variations

Alternative sequence1 – 6262Missing in isoform c. Ref.4
VSP_051940
Alternative sequence370 – 3712Missing in isoform a and isoform c. Ref.1 Ref.4
VSP_051941

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: D1401DB1286D8624

FASTA62670,443
        10         20         30         40         50         60 
MFSHQDRDRD RKEDGGGDGT EMKSKSRSQP SGLNRVKNLS RKLSAKSRKE RKDRDSTDNS 

        70         80         90        100        110        120 
SKMSSPGGET STKQQQELKA QIKIGHYILK ETLGVGTFGK VKVGIHETTQ YKVAVKILNR 

       130        140        150        160        170        180 
QKIKSLDVVG KIRREIQNLS LFRHPHIIRL YQVISTPSDI FMIMEHVSGG ELFDYIVKHG 

       190        200        210        220        230        240 
RLKTAEARRF FQQIISGVDY CHRHMVVHRD LKPENLLLDE QNNVKIADFG LSNIMTDGDF 

       250        260        270        280        290        300 
LRTSCGSPNY AAPEVISGKL YAGPEVDVWS CGVILYALLC GTLPFDDEHV PSLFRKIKSG 

       310        320        330        340        350        360 
VFPTPDFLER PIVNLLHHML CVDPMKRATI KDVIAHEWFQ KDLPNYLFPP INESEASIVD 

       370        380        390        400        410        420 
IEAVREVTEF QRYHVAEEEV TSALLGDDPH HHLSIAYNLI VDNKRIADET AKLSIEEFYQ 

       430        440        450        460        470        480 
VTPNKGPGPV HRHPERIAAS VSSKITPTLD NTEASGANRN KRAKWHLGIR SQSRPEDIMF 

       490        500        510        520        530        540 
EVFRAMKQLD MEWKVLNPYH VIVRRKPDAP AADPPKMSLQ LYQVDQRSYL LDFKSLADEE 

       550        560        570        580        590        600 
SGSASASSSR HASMSMPQKP AGIRGTRTSS MPQAMSMEAS IEKMEVHDFS DMSCDVTPPP 

       610        620 
SPGGAKLSQT MQFFEICAAL IGTLAR 

« Hide

Isoform a.

Checksum: 3BF9F9610BA95DB7
Show »

FASTA62470,168
Isoform c.

Checksum: AA34A96C5BBCAFBD
Show »

FASTA56263,103

References

« Hide 'large scale' references
[1]"The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans."
Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.
Genes Dev. 18:3004-3009(2004) [PubMed: 15574588] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, ENZYME REGULATION.
[2]Erratum
Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.
Genes Dev. 19:188-188(2005)
[3]Erratum
Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.
Genes Dev. 19:411-411(2005)
[4]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

AY347273 mRNA. Translation: AAR06928.1.
U58726 Genomic DNA. Translation: AAM69095.1.
U58726 Genomic DNA. Translation: AAM69096.1.
U58726 Genomic DNA. Translation: AAP13770.1.
PIRT29858.
RefSeqNP_510710.2.
UniGeneCel.17479

3D structure databases

HSSPHSSP built from PDB template 1NXK based on UniProtKB P49137.
SMRQ95ZQ4. Positions 81-349.
ModBaseSearch...

Protein-protein interaction databases

IntActQ95ZQ4. 9 interactions.

Proteomic databases

PRIDEQ95ZQ4.

Genome annotation databases

EnsemblT01C8.1. Caenorhabditis elegans. [Contig view]
GeneID181727.

Organism-specific databases

WormBaseWBGene00020142. aak-2.
WormPepT01C8.1a. CE31222. [WorfDB]
T01C8.1b. CE31223. [WorfDB]
T01C8.1c. CE07458. [WorfDB]

Phylogenomic databases

OMAQ95ZQ4. MKRATIR.

Enzyme and pathway databases

BRENDA2.7.11.1. 672.

Gene expression databases

ArrayExpressQ95ZQ4.

Family and domain databases

InterProIPR015741. AMPK.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PANTHERPTHR22982:SF61. AMPK. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio915106.

Entry information

Entry nameAAPK2_CAEEL
AccessionPrimary (citable) accession number: Q95ZQ4
Secondary accession number(s): Q22068, Q86FL6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents