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Q95YH0

- CDK8_DICDI

UniProt

Q95YH0 - CDK8_DICDI

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Protein
Probable cyclin-dependent kinase 8
Gene
cdk8, DDB_G0267442
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity. Involved in cell aggregation, but not growth. Required for starvation-induced expression of genes essential for early development of acaA and/or other genes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741ATP By similarity
Active sitei172 – 1721Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 589ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: dictyBase
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: dictyBase
  3. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  4. cyclin-dependent protein serine/threonine kinase regulator activity Source: dictyBase

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: UniProtKB
  2. aggregation involved in sorocarp development Source: dictyBase
  3. cell-cell adhesion involved in flocculation Source: UniProtKB
  4. phosphorylation of RNA polymerase II C-terminal domain Source: dictyBase
  5. protein phosphorylation Source: UniProtKB
  6. protein stabilization Source: dictyBase
  7. regulation of aggregation involved in sorocarp development Source: dictyBase
  8. regulation of protein kinase activity Source: GOC
  9. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Ddcdk8
Gene namesi
Name:cdk8
ORF Names:DDB_G0267442
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267442. cdk8.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. DNA-directed RNA polymerase II, holoenzyme Source: dictyBase
  2. cytosol Source: dictyBase
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Cells exhibit slow growth, they do not aggregate upon starvation and form small plaques on bacterial lawns.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Probable cyclin-dependent kinase 8
PRO_0000328274Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed at a relatively low level during growth, increased 80-fold during early development, with a maximum during the growth phase at 12 hours, and quickly declined fourfold during 12 and 15 hours.1 Publication

Interactioni

Subunit structurei

Component of the Mediator complex By similarity.

Protein-protein interaction databases

STRINGi44689.DDB_0191261.

Structurei

3D structure databases

ProteinModelPortaliQ95YH0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 348305Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 2312Poly-Gly
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
KOiK02208.
OMAiTPITANC.
PhylomeDBiQ95YH0.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95YH0-1 [UniParc]FASTAAdd to Basket

« Hide

MNTHNQSSNQ NGGGSGGGGG GGGSSTFSPI VLTSTYRLDV QEKYTFSYEI    50
GSGTYGMVYK ADDKKRPNNK VAVKKFRSTK EGEGLSLTAY REIGLLKELS 100
NENIVKLLDV CLNPKDKLLY LIFDYAEFDL FGIIKYHREN GSHFSDATIK 150
SLIWQVLNGI HYLHSNWVIH RDLKPSNILV MGEGKECGTV KIGDFGLARI 200
FQSPLKPLNE NGVVVTIWYR SPELLLGSKH YTRAVDIWAI GCIFAELITT 250
KPLFPGKEKD PKIPSLFQDD QVEKIIRVLG KPTLDMWPDI KHLPEWKRLS 300
SMEAFPNSLA KCVGIDENSQ AYDLLSKMIL YDPSKRITAS EALDHPYFKE 350
LPLPLPNAFS KPIPYPPRLP INKKKREFDD 380
Length:380
Mass (Da):42,771
Last modified:December 1, 2001 - v1
Checksum:iF53BBC1D68E447E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071894 mRNA. Translation: BAB68407.1.
AAFI02000003 Genomic DNA. Translation: EAL73173.1.
RefSeqiXP_647600.1. XM_642508.1.

Genome annotation databases

EnsemblProtistsiDDB0191261; DDB0191261; DDB_G0267442.
GeneIDi8616412.
KEGGiddi:DDB_G0267442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071894 mRNA. Translation: BAB68407.1 .
AAFI02000003 Genomic DNA. Translation: EAL73173.1 .
RefSeqi XP_647600.1. XM_642508.1.

3D structure databases

ProteinModelPortali Q95YH0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191261 ; DDB0191261 ; DDB_G0267442 .
GeneIDi 8616412.
KEGGi ddi:DDB_G0267442.

Organism-specific databases

dictyBasei DDB_G0267442. cdk8.

Phylogenomic databases

eggNOGi COG0515.
KOi K02208.
OMAi TPITANC.
PhylomeDBi Q95YH0.

Miscellaneous databases

PROi Q95YH0.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth."
    Takeda K., Saito T., Ochiai H.
    Dev. Growth Differ. 44:213-223(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: AX2.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCDK8_DICDI
AccessioniPrimary (citable) accession number: Q95YH0
Secondary accession number(s): Q55FD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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