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Q95YH0

- CDK8_DICDI

UniProt

Q95YH0 - CDK8_DICDI

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Protein

Probable cyclin-dependent kinase 8

Gene

cdk8

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity). Involved in cell aggregation, but not growth. Required for starvation-induced expression of genes essential for early development of acaA and/or other genes.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741ATPPROSITE-ProRule annotation
Active sitei172 – 1721Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 589ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: dictyBase
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. cyclin-dependent protein serine/threonine kinase regulator activity Source: dictyBase
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: dictyBase

GO - Biological processi

  1. aggregation involved in sorocarp development Source: dictyBase
  2. cell-cell adhesion involved in flocculation Source: UniProtKB
  3. G1/S transition of mitotic cell cycle Source: UniProtKB
  4. phosphorylation of RNA polymerase II C-terminal domain Source: dictyBase
  5. positive regulation of gene expression Source: dictyBase
  6. protein phosphorylation Source: UniProtKB
  7. protein stabilization Source: dictyBase
  8. regulation of aggregation involved in sorocarp development Source: dictyBase
  9. regulation of protein kinase activity Source: GOC
  10. sorocarp spore cell differentiation Source: dictyBase
  11. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Ddcdk8
Gene namesi
Name:cdk8
ORF Names:DDB_G0267442
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267442. cdk8.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytosol Source: dictyBase
  2. DNA-directed RNA polymerase II, holoenzyme Source: dictyBase
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Cells exhibit slow growth, they do not aggregate upon starvation and form small plaques on bacterial lawns.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Probable cyclin-dependent kinase 8PRO_0000328274Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed at a relatively low level during growth, increased 80-fold during early development, with a maximum during the growth phase at 12 hours, and quickly declined fourfold during 12 and 15 hours.1 Publication

Interactioni

Subunit structurei

Component of the Mediator complex.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0191261.

Structurei

3D structure databases

ProteinModelPortaliQ95YH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 348305Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 2312Poly-GlyAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiQ95YH0.
KOiK02208.
OMAiTPITANC.
PhylomeDBiQ95YH0.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95YH0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTHNQSSNQ NGGGSGGGGG GGGSSTFSPI VLTSTYRLDV QEKYTFSYEI
60 70 80 90 100
GSGTYGMVYK ADDKKRPNNK VAVKKFRSTK EGEGLSLTAY REIGLLKELS
110 120 130 140 150
NENIVKLLDV CLNPKDKLLY LIFDYAEFDL FGIIKYHREN GSHFSDATIK
160 170 180 190 200
SLIWQVLNGI HYLHSNWVIH RDLKPSNILV MGEGKECGTV KIGDFGLARI
210 220 230 240 250
FQSPLKPLNE NGVVVTIWYR SPELLLGSKH YTRAVDIWAI GCIFAELITT
260 270 280 290 300
KPLFPGKEKD PKIPSLFQDD QVEKIIRVLG KPTLDMWPDI KHLPEWKRLS
310 320 330 340 350
SMEAFPNSLA KCVGIDENSQ AYDLLSKMIL YDPSKRITAS EALDHPYFKE
360 370 380
LPLPLPNAFS KPIPYPPRLP INKKKREFDD
Length:380
Mass (Da):42,771
Last modified:December 1, 2001 - v1
Checksum:iF53BBC1D68E447E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071894 mRNA. Translation: BAB68407.1.
AAFI02000003 Genomic DNA. Translation: EAL73173.1.
RefSeqiXP_647600.1. XM_642508.1.

Genome annotation databases

EnsemblProtistsiDDB0191261; DDB0191261; DDB_G0267442.
GeneIDi8616412.
KEGGiddi:DDB_G0267442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071894 mRNA. Translation: BAB68407.1 .
AAFI02000003 Genomic DNA. Translation: EAL73173.1 .
RefSeqi XP_647600.1. XM_642508.1.

3D structure databases

ProteinModelPortali Q95YH0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191261 ; DDB0191261 ; DDB_G0267442 .
GeneIDi 8616412.
KEGGi ddi:DDB_G0267442.

Organism-specific databases

dictyBasei DDB_G0267442. cdk8.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi Q95YH0.
KOi K02208.
OMAi TPITANC.
PhylomeDBi Q95YH0.

Miscellaneous databases

PROi Q95YH0.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth."
    Takeda K., Saito T., Ochiai H.
    Dev. Growth Differ. 44:213-223(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: AX2.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCDK8_DICDI
AccessioniPrimary (citable) accession number: Q95YH0
Secondary accession number(s): Q55FD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3