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Q95YH0 (CDK8_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cyclin-dependent kinase 8

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Ddcdk8
Gene names
Name:cdk8
ORF Names:DDB_G0267442
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity. Involved in cell aggregation, but not growth. Required for starvation-induced expression of genes essential for early development of acaA and/or other genes. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the Mediator complex By similarity.

Subcellular location

Nucleus Probable.

Developmental stage

Expressed at a relatively low level during growth, increased 80-fold during early development, with a maximum during the growth phase at 12 hours, and quickly declined fourfold during 12 and 15 hours. Ref.1

Disruption phenotype

Cells exhibit slow growth, they do not aggregate upon starvation and form small plaques on bacterial lawns. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionActivator
Kinase
Repressor
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG1/S transition of mitotic cell cycle

Inferred from sequence or structural similarity. Source: UniProtKB

aggregation involved in sorocarp development

Inferred from mutant phenotype Ref.1PubMed 15196949. Source: dictyBase

cell-cell adhesion involved in flocculation

Inferred from mutant phenotype Ref.1. Source: UniProtKB

phosphorylation of RNA polymerase II C-terminal domain

Inferred from direct assay PubMed 15196949. Source: dictyBase

protein phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

protein stabilization

Inferred from mutant phenotype PubMed 20479885. Source: dictyBase

regulation of aggregation involved in sorocarp development

Inferred from mutant phenotype PubMed 20479885. Source: dictyBase

regulation of protein kinase activity

Inferred from sequence or structural similarity. Source: GOC

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentDNA-directed RNA polymerase II, holoenzyme

Inferred from sequence or structural similarity Ref.1. Source: dictyBase

cytosol

Inferred from direct assay PubMed 15196949. Source: dictyBase

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred by curator PubMed 15196949. Source: dictyBase

RNA polymerase II carboxy-terminal domain kinase activity

Inferred from direct assay PubMed 15196949. Source: dictyBase

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cyclin-dependent protein serine/threonine kinase regulator activity

Inferred from sequence or structural similarity. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 380380Probable cyclin-dependent kinase 8
PRO_0000328274

Regions

Domain44 – 348305Protein kinase
Nucleotide binding50 – 589ATP By similarity
Compositional bias12 – 2312Poly-Gly

Sites

Active site1721Proton acceptor By similarity
Binding site741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q95YH0 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F53BBC1D68E447E8

FASTA38042,771
        10         20         30         40         50         60 
MNTHNQSSNQ NGGGSGGGGG GGGSSTFSPI VLTSTYRLDV QEKYTFSYEI GSGTYGMVYK 

        70         80         90        100        110        120 
ADDKKRPNNK VAVKKFRSTK EGEGLSLTAY REIGLLKELS NENIVKLLDV CLNPKDKLLY 

       130        140        150        160        170        180 
LIFDYAEFDL FGIIKYHREN GSHFSDATIK SLIWQVLNGI HYLHSNWVIH RDLKPSNILV 

       190        200        210        220        230        240 
MGEGKECGTV KIGDFGLARI FQSPLKPLNE NGVVVTIWYR SPELLLGSKH YTRAVDIWAI 

       250        260        270        280        290        300 
GCIFAELITT KPLFPGKEKD PKIPSLFQDD QVEKIIRVLG KPTLDMWPDI KHLPEWKRLS 

       310        320        330        340        350        360 
SMEAFPNSLA KCVGIDENSQ AYDLLSKMIL YDPSKRITAS EALDHPYFKE LPLPLPNAFS 

       370        380 
KPIPYPPRLP INKKKREFDD 

« Hide

References

« Hide 'large scale' references
[1]"A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth."
Takeda K., Saito T., Ochiai H.
Dev. Growth Differ. 44:213-223(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
Strain: AX2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB071894 mRNA. Translation: BAB68407.1.
AAFI02000003 Genomic DNA. Translation: EAL73173.1.
RefSeqXP_647600.1. XM_642508.1.

3D structure databases

ProteinModelPortalQ95YH0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191261; DDB0191261; DDB_G0267442.
GeneID8616412.
KEGGddi:DDB_G0267442.

Organism-specific databases

dictyBaseDDB_G0267442. cdk8.

Phylogenomic databases

eggNOGCOG0515.
OMATPITANC.
PhylomeDBQ95YH0.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ95YH0.

Entry information

Entry nameCDK8_DICDI
AccessionPrimary (citable) accession number: Q95YH0
Secondary accession number(s): Q55FD3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase