Q95YH0 (CDK8_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cyclin-dependent kinase 8
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Ddcdk8

Gene names

Name:	cdk8
ORF Names:	DDB_G0267442

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity. Involved in cell aggregation, but not growth. Required for starvation-induced expression of genes essential for early development of acaA and/or other genes. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Developmental stage	Expressed at a relatively low level during growth, increased 80-fold during early development, with a maximum during the growth phase at 12 hours, and quickly declined fourfold during 12 and 15 hours. Ref.1
Disruption phenotype	Cells exhibit slow growth, they do not aggregate upon starvation and form small plaques on bacterial lawns. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB aggregation involved in sorocarp development Inferred from mutant phenotype Ref.1PubMed 15196949. Source: dictyBase cell-cell adhesion involved in flocculation Inferred from mutant phenotype Ref.1. Source: UniProtKB phosphorylation of RNA polymerase II C-terminal domain Inferred from direct assay PubMed 15196949. Source: dictyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA-directed RNA polymerase II, holoenzyme Inferred from sequence or structural similarity Ref.1. Source: dictyBase cytosol Inferred from direct assay PubMed 15196949. Source: dictyBase extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase
Molecular_function	ATP binding Inferred by curator PubMed 15196949. Source: dictyBase RNA polymerase II carboxy-terminal domain kinase activity Inferred from direct assay PubMed 15196949. Source: dictyBase cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase regulator activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Probable cyclin-dependent kinase 8		PRO_0000328274
Regions
	Domain	44 – 348	305	Protein kinase
	Nucleotide binding	50 – 58	9	ATP By similarity
	Compositional bias	12 – 23	12	Poly-Gly
Sites
	Active site	172	1	Proton acceptor By similarity
	Binding site	74	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95YH0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F53BBC1D68E447E8	FASTA	380	42,771
	10 20 30 40 50 60 MNTHNQSSNQ NGGGSGGGGG GGGSSTFSPI VLTSTYRLDV QEKYTFSYEI GSGTYGMVYK 70 80 90 100 110 120 ADDKKRPNNK VAVKKFRSTK EGEGLSLTAY REIGLLKELS NENIVKLLDV CLNPKDKLLY 130 140 150 160 170 180 LIFDYAEFDL FGIIKYHREN GSHFSDATIK SLIWQVLNGI HYLHSNWVIH RDLKPSNILV 190 200 210 220 230 240 MGEGKECGTV KIGDFGLARI FQSPLKPLNE NGVVVTIWYR SPELLLGSKH YTRAVDIWAI 250 260 270 280 290 300 GCIFAELITT KPLFPGKEKD PKIPSLFQDD QVEKIIRVLG KPTLDMWPDI KHLPEWKRLS 310 320 330 340 350 360 SMEAFPNSLA KCVGIDENSQ AYDLLSKMIL YDPSKRITAS EALDHPYFKE LPLPLPNAFS 370 380 KPIPYPPRLP INKKKREFDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth." Takeda K., Saito T., Ochiai H. Dev. Growth Differ. 44:213-223(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB071894 mRNA. Translation: BAB68407.1. AAFI02000003 Genomic DNA. Translation: EAL73173.1.
RefSeq	XP_647600.1. XM_642508.1.
3D structure databases
HSSP	HSSP built from PDB template 1PYE based on UniProtKB P24941.
ProteinModelPortal	Q95YH0.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191261.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191261; DDB0191261; DDB_G0267442.
GeneID	8616412.
KEGG	ddi:DDB_G0267442.
Organism-specific databases
dictyBase	DDB_G0267442. cdk8.
Phylogenomic databases
eggNOG	COG0515.
KO	K02208.
OMA	ADMSLYL.
ProtClustDB	CLSZ2729373.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK8_DICDI

Accession

Primary (citable) accession number: Q95YH0
Secondary accession number(s): Q55FD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)