Q95Y12 (SET23_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable histone-lysine N-methyltransferase set-23
EC=2.1.1.43

Alternative name(s):
SET-domain containing protein 23

Gene names

Name:	set-23
ORF Names:	Y41D4B.12

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probable histone methyltransferase By similarity. Required for embryonic development. Ref.2
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus By similarity. Chromosome By similarity.
Disruption phenotype	Embryonic lethal. Ref.2
Sequence similarities	Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Developmental protein Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q95Y12-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q95Y12-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     86-95: GPQKKLEIFS → MCLHTAPNFI

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Probable histone-lysine N-methyltransferase set-23		PRO_0000287565
Regions
	Domain	25 – 86	62	Pre-SET
	Domain	88 – 217	130	SET
	Domain	221 – 237	17	Post-SET
Natural variations
	Alternative sequence	1 – 85	85	Missing in isoform b.		VSP_025564
	Alternative sequence	86 – 95	10	GPQKKLEIFS → MCLHTAPNFI in isoform b.		VSP_025565

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AB291915D129C2A3	FASTA	244	27,108
	10 20 30 40 50 60 MNYEKIDSTI PGPGISETDW NDVFEGCNCE AECSSAAGCS CLINKIDNYT VDGKINKSSE 70 80 90 100 110 120 LLIECSDQCA CILLPTSCRN RVVQCGPQKK LEIFSTCEMA KGFGVRAGEQ IAAGEFVCEY 130 140 150 160 170 180 AGECIGEQEV ERRCREFRGD DNYTLTLKEF FGGKPVKTFV DPRLRGNIGR FLNHSCEPNC 190 200 210 220 230 240 EIILARLGRM IPAAGIFAKR DIVRGEELCY DYGHSAIEGE NRKLCLCKSE KCRKYLPMSA TPIE
	Isoform b [UniParc]. Checksum: AF09BB931CC5CA44	FASTA	159	17,895
	10 20 30 40 50 60 MCLHTAPNFI TCEMAKGFGV RAGEQIAAGE FVCEYAGECI GEQEVERRCR EFRGDDNYTL 70 80 90 100 110 120 TLKEFFGGKP VKTFVDPRLR GNIGRFLNHS CEPNCEIILA RLGRMIPAAG IFAKRDIVRG 130 140 150 EELCYDYGHS AIEGENRKLC LCKSEKCRKY LPMSATPIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Two C. elegans histone methyltransferases repress lin-3 EGF transcription to inhibit vulval development." Andersen E.C., Horvitz H.R. Development 134:2991-2999(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080782 Genomic DNA. Translation: CCD66712.1. FO080782 Genomic DNA. Translation: CCD66713.1.
RefSeq	NP_741320.1. NM_171270.1. NP_741321.1. NM_171271.3.
UniGene	Cel.32662.
3D structure databases
ProteinModelPortal	Q95Y12.
SMR	Q95Y12. Positions 10-241.
ModBase	Search...
Proteomic databases
PRIDE	Q95Y12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y41D4B.12a; Y41D4B.12a; Y41D4B.12.
GeneID	176969.
KEGG	cel:CELE_Y41D4B.12.
Organism-specific databases
CTD	176969.
WormBase	Y41D4B.12a; CE27623; WBGene00021515; set-23. Y41D4B.12b; CE31647; WBGene00021515; set-23.
Phylogenomic databases
eggNOG	COG2940.
GeneTree	ENSGT00700000104009.
InParanoid	Q95Y12.
KO	K11433.
OMA	QEVERRC.
Family and domain databases
InterPro	IPR003616. Post-SET_dom. IPR007728. Pre-SET_dom. IPR001214. SET_dom. [Graphical view]
Pfam	PF05033. Pre-SET. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50868. POST_SET. 1 hit. PS50867. PRE_SET. 1 hit. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	894788.

Entry information

Entry name

SET23_CAEEL

Accession

Primary (citable) accession number: Q95Y12
Secondary accession number(s): Q8MXT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project