Q95X01 (MYRO1_BREBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myrosinase 1 EC=3.2.1.147 Alternative name(s): Beta-glucosidase 1 Beta-thioglucosidase 1 Beta-thioglucosidase glucohydrolase 1 Sinigrinase 1 Thioglucosidase 1
Organism	Brevicoryne brassicae (Cabbage aphid)
Taxonomic identifier	69196 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Paraneoptera › Hemiptera › Sternorrhyncha › Aphidiformes › Aphidomorpha › Aphidoidea › Aphididae › Aphidinae › Macrosiphini › Brevicoryne

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes glucosinolates to a labile aglcone. This rapidly undergoes spontaneous rearrangement, eliminating sulfur to yield a number of toxic metabolites. Thereby developing a chemical defense system that exploits and mimics the host plant. Ref.1 Ref.5
Catalytic activity	A thioglucoside + H2O = a sugar + a thiol. Ref.1
Subunit structure	Homodimer. Ref.2
Tissue specificity	Expressed in the skeletal muscle tissues surrounding the head, abdomen and thorax. Not expressed in flight muscles (at protein level). Ref.4 Ref.5
Developmental stage	Expression in the head and thoracic muscle starts during embryonic development and levels continue to accumulate after the nymphs are born (at protein level). Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 1 family. Ref.1
Biophysicochemical properties	Kinetic parameters: KM=0.613 mM for allylglucosinolate Ref.3 KM=0.915 mM for benzylglucosinolate Ref.3 pH dependence: Optimum pH is 5.5 with sinigrin as substrate. Ref.3
Mass spectrometry	Molecular mass is 54000±500 Da from positions 1 - 464. Determined by MALDI. Ref.3

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thioglucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 464

464

Myrosinase 1

PRO_0000392945

Sites

Active site

167

1

Nucleophile Ref.2

Active site

374

1

Proton donor Ref.2

Metal binding

39

1

Zinc; shared with dimeric partner By similarity UniProtKB P29736

Metal binding

52

1

Zinc; shared with dimeric partner By similarity UniProtKB P29736

Binding site

19

1

Substrate By similarity UniProtKB P29736

Binding site

122

1

Substrate By similarity UniProtKB P29736

Binding site

166

1

Substrate By similarity UniProtKB P29736

Amino acid modifications

Glycosylation

397

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

107

1

N → P AA sequence Ref.3

Sequence conflict

322

1

P → N AA sequence Ref.3

Secondary structure

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464

Helix Strand Turn

Details...

Beta strand

10 – 14

5

Helix

17 – 20

4

Helix

33 – 40

8

Helix

42 – 44

3

Beta strand

51 – 53

3

Helix

57 – 71

15

Beta strand

74 – 79

6

Helix

82 – 85

4

Helix

96 – 111

16

Beta strand

115 – 123

9

Helix

127 – 131

5

Helix

134 – 136

3

Helix

140 – 155

16

Turn

156 – 158

3

Beta strand

161 – 166

6

Helix

168 – 176

9

Beta strand

177 – 180

4

Helix

187 – 212

26

Helix

214 – 217

4

Beta strand

220 – 225

6

Beta strand

229 – 234

6

Helix

238 – 251

14

Helix

253 – 260

8

Beta strand

262 – 264

3

Helix

266 – 278

13

Helix

291 – 297

7

Beta strand

302 – 307

6

Beta strand

311 – 317

7

Helix

326 – 328

3

Beta strand

330 – 333

4

Helix

336 – 338

3

Helix

351 – 365

15

Beta strand

370 – 375

6

Helix

386 – 406

21

Beta strand

410 – 416

7

Helix

424 – 429

6

Beta strand

434 – 437

4

Beta strand

446 – 448

3

Helix

450 – 461

12

Sequences

	Sequence		Length	Mass (Da)
	Q95X01 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 02E76E24504C393A	FASTA	464	53,736
	10 20 30 40 50 60 MDYKFPKDFM FGTSTASYQI EGGWNEDGKG ENIWDRLVHT SPEVIKDGTN GDIACDSYHK 70 80 90 100 110 120 YKEDVAIIKD LNLKFYRFSI SWARIAPSGV MNSLEPKGIA YYNNLINELI KNDIIPLVTM 130 140 150 160 170 180 YHWDLPQYLQ DLGGWVNPIM SDYFKEYARV LFTYFGDRVK WWITFNEPIA VCKGYSIKAY 190 200 210 220 230 240 APNLNLKTTG HYLAGHTQLI AHGKAYRLYE EMFKPTQNGK ISISISGVFF MPKNAESDDD 250 260 270 280 290 300 IETAERANQF ERGWFGHPVY KGDYPPIMKK WVDQKSKEEG LPWSKLPKFT KDEIKLLKGT 310 320 330 340 350 360 ADFYALNHYS SRLVTFGSDP NPNFNPDASY VTSVDEAWLK PNETPYIIPV PEGLRKLLIW 370 380 390 400 410 420 LKNEYGNPQL LITENGYGDD GQLDDFEKIS YLKNYLNATL QAMYEDKCNV IGYTVWSLLD 430 440 450 460 NFEWFYGYSI HFGLVKIDFN DPQRTRTKRE SYTYFKNVVS TGKP

References

[1]	"Characterization and evolution of a myrosinase from the cabbage aphid Brevicoryne brassicae." Jones A.M., Winge P., Bones A.M., Cole R., Rossiter J.T. Insect Biochem. Mol. Biol. 32:275-284(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PROTEIN SEQUENCE OF 98-111; 188-197; 253-261; 313-327 AND 389-393.
[2]	"Crystal structure at 1.1 Angstroms resolution of an insect myrosinase from Brevicoryne brassicae shows its close relationship to beta-glucosidases." Husebye H., Arzt S., Burmeister W.P., Hartel F.V., Brandt A., Rossiter J.T., Bones A.M. Insect Biochem. Mol. Biol. 35:1311-1320(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, ACTIVE SITE GLU-167, ACTIVE SITE GLU-374, X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
[3]	"Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.)." Jones A.M., Bridges M., Bones A.M., Cole R., Rossiter J.T. Insect Biochem. Mol. Biol. 31:1-5(2001) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY, PROTEIN SEQUENCE OF 98-111; 188-197; 253-261; 313-327 AND 389-393.
[4]	"Spatial organization of the glucosinolate-myrosinase system in brassica specialist aphids is similar to that of the host plant." Bridges M., Jones A.M., Bones A.M., Hodgson C., Cole R., Bartlet E., Wallsgrove R., Karapapa V.K., Watts N., Rossiter J.T. Proc. R. Soc. B 269:187-191(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"The cabbage aphid: a walking mustard oil bomb." Kazana E., Pope T.W., Tibbles L., Bridges M., Pickett J.A., Bones A.M., Powell G., Rossiter J.T. Proc. R. Soc. B 274:2271-2277(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF203780 mRNA. Translation: AAL25999.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WCG	X-ray	1.10	A/B	1-464	[»]

ProteinModelPortal

Q95X01.

SMR

Q95X01. Positions 3-464.

ModBase

Search...

Protein family/group databases

CAZy

GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.20.20.80. 1 hit.

InterPro

IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

PANTHER

PTHR10353. PTHR10353. 1 hit.

Pfam

PF00232. Glyco_hydro_1. 1 hit.
[Graphical view]

PRINTS

PR00131. GLHYDRLASE1.

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00572. GLYCOSYL_HYDROL_F1_1. False negative.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q95X01.

Entry information

Entry name

MYRO1_BREBR

Accession

Primary (citable) accession number: Q95X01

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)