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Q95X01

- MYRO1_BREBR

UniProt

Q95X01 - MYRO1_BREBR

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Protein

Myrosinase 1

Gene
N/A
Organism
Brevicoryne brassicae (Cabbage aphid)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes glucosinolates to a labile aglycone. This rapidly undergoes spontaneous rearrangement, eliminating sulfur to yield a number of toxic metabolites. Thereby developing a chemical defense system that exploits and mimics the host plant.2 Publications

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.1 Publication

Kineticsi

  1. KM=0.613 mM for allylglucosinolate1 Publication
  2. KM=0.915 mM for benzylglucosinolate1 Publication

pH dependencei

Optimum pH is 5.5 with sinigrin as substrate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191SubstrateBy similarity
Metal bindingi39 – 391Zinc; shared with dimeric partnerBy similarity
Metal bindingi52 – 521Zinc; shared with dimeric partnerBy similarity
Binding sitei122 – 1221SubstrateBy similarity
Binding sitei166 – 1661SubstrateBy similarity
Active sitei167 – 1671Nucleophile1 Publication
Active sitei374 – 3741Proton donor1 Publication

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. thioglucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 11 Publication (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 11 Publication
Beta-thioglucosidase 11 Publication
Beta-thioglucosidase glucohydrolase 11 Publication
Sinigrinase 11 Publication
Thioglucosidase 1Imported
OrganismiBrevicoryne brassicae (Cabbage aphid)
Taxonomic identifieri69196 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidiformesAphidoideaAphididaeMacrosiphiniBrevicoryne

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Myrosinase 1PRO_0000392945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in the skeletal muscle tissues surrounding the head, abdomen and thorax. Not expressed in flight muscles (at protein level).2 Publications

Developmental stagei

Expression in the head and thoracic muscle starts during embryonic development and levels continue to accumulate after the nymphs are born (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
464
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 145Combined sources
Helixi17 – 204Combined sources
Helixi33 – 408Combined sources
Helixi42 – 443Combined sources
Beta strandi51 – 533Combined sources
Helixi57 – 7115Combined sources
Beta strandi74 – 796Combined sources
Helixi82 – 854Combined sources
Helixi96 – 11116Combined sources
Beta strandi115 – 1239Combined sources
Helixi127 – 1315Combined sources
Helixi134 – 1363Combined sources
Helixi140 – 15516Combined sources
Turni156 – 1583Combined sources
Beta strandi161 – 1666Combined sources
Helixi168 – 1769Combined sources
Beta strandi177 – 1804Combined sources
Helixi187 – 21226Combined sources
Helixi214 – 2174Combined sources
Beta strandi220 – 2256Combined sources
Beta strandi229 – 2346Combined sources
Helixi238 – 25114Combined sources
Helixi253 – 2608Combined sources
Beta strandi262 – 2643Combined sources
Helixi266 – 27813Combined sources
Helixi291 – 2977Combined sources
Beta strandi302 – 3076Combined sources
Beta strandi311 – 3177Combined sources
Helixi326 – 3283Combined sources
Beta strandi330 – 3334Combined sources
Helixi336 – 3383Combined sources
Helixi351 – 36515Combined sources
Beta strandi370 – 3756Combined sources
Helixi386 – 40621Combined sources
Beta strandi410 – 4167Combined sources
Helixi424 – 4296Combined sources
Beta strandi434 – 4374Combined sources
Beta strandi446 – 4483Combined sources
Helixi450 – 46112Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WCGX-ray1.10A/B1-464[»]
ProteinModelPortaliQ95X01.
SMRiQ95X01. Positions 3-464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ95X01.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.1 Publication

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95X01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKFPKDFM FGTSTASYQI EGGWNEDGKG ENIWDRLVHT SPEVIKDGTN
60 70 80 90 100
GDIACDSYHK YKEDVAIIKD LNLKFYRFSI SWARIAPSGV MNSLEPKGIA
110 120 130 140 150
YYNNLINELI KNDIIPLVTM YHWDLPQYLQ DLGGWVNPIM SDYFKEYARV
160 170 180 190 200
LFTYFGDRVK WWITFNEPIA VCKGYSIKAY APNLNLKTTG HYLAGHTQLI
210 220 230 240 250
AHGKAYRLYE EMFKPTQNGK ISISISGVFF MPKNAESDDD IETAERANQF
260 270 280 290 300
ERGWFGHPVY KGDYPPIMKK WVDQKSKEEG LPWSKLPKFT KDEIKLLKGT
310 320 330 340 350
ADFYALNHYS SRLVTFGSDP NPNFNPDASY VTSVDEAWLK PNETPYIIPV
360 370 380 390 400
PEGLRKLLIW LKNEYGNPQL LITENGYGDD GQLDDFEKIS YLKNYLNATL
410 420 430 440 450
QAMYEDKCNV IGYTVWSLLD NFEWFYGYSI HFGLVKIDFN DPQRTRTKRE
460
SYTYFKNVVS TGKP
Length:464
Mass (Da):53,736
Last modified:December 1, 2001 - v1
Checksum:i02E76E24504C393A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071N → P AA sequence (PubMed:11102829)Curated
Sequence conflicti322 – 3221P → N AA sequence (PubMed:11102829)Curated

Mass spectrometryi

Molecular mass is 54000±500 Da from positions 1 - 464. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203780 mRNA. Translation: AAL25999.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203780 mRNA. Translation: AAL25999.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WCG X-ray 1.10 A/B 1-464 [» ]
ProteinModelPortali Q95X01.
SMRi Q95X01. Positions 3-464.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q95X01.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization and evolution of a myrosinase from the cabbage aphid Brevicoryne brassicae."
    Jones A.M., Winge P., Bones A.M., Cole R., Rossiter J.T.
    Insect Biochem. Mol. Biol. 32:275-284(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PROTEIN SEQUENCE OF 98-111; 188-197; 253-261; 313-327 AND 389-393.
  2. "Crystal structure at 1.1 Angstroms resolution of an insect myrosinase from Brevicoryne brassicae shows its close relationship to beta-glucosidases."
    Husebye H., Arzt S., Burmeister W.P., Hartel F.V., Brandt A., Rossiter J.T., Bones A.M.
    Insect Biochem. Mol. Biol. 35:1311-1320(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, ACTIVE SITE GLU-167, ACTIVE SITE GLU-374, X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
  3. "Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.)."
    Jones A.M., Bridges M., Bones A.M., Cole R., Rossiter J.T.
    Insect Biochem. Mol. Biol. 31:1-5(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY, PROTEIN SEQUENCE OF 98-111; 188-197; 253-261; 313-327 AND 389-393.
  4. "Spatial organization of the glucosinolate-myrosinase system in brassica specialist aphids is similar to that of the host plant."
    Bridges M., Jones A.M., Bones A.M., Hodgson C., Cole R., Bartlet E., Wallsgrove R., Karapapa V.K., Watts N., Rossiter J.T.
    Proc. R. Soc. B 269:187-191(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMYRO1_BREBR
AccessioniPrimary (citable) accession number: Q95X01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3