Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box-like/WD repeat-containing protein ebi

Gene

ebi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

F-box-like component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, cone cell development and neuronal cell cycle control. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Required to block the S phase entry in the peripheral nervous system and central nervous system in a process that does not involve the degradation of ttk. Involved in cone cell development by preventing the transcriptional repression mediated by Su(H) on Dl, probably by participating in a E3 complex that contains sno and mediates the ubiquitination and subsequent proteasomal degradation of some component of the Su(H) repressor complex.3 Publications

Miscellaneous

'Ebi' means 'shrimp' in Japanese.

GO - Molecular functioni

  • chromatin binding Source: FlyBase
  • repressing transcription factor binding Source: FlyBase

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to light stimulus Source: FlyBase
  • chaeta development Source: FlyBase
  • compound eye cone cell fate commitment Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • negative regulation of JNK cascade Source: FlyBase
  • negative regulation of neuron apoptotic process Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription by RNA polymerase II Source: GOC
  • neuron cellular homeostasis Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • photoreceptor cell development Source: FlyBase
  • positive regulation of histone deacetylation Source: FlyBase
  • positive regulation of imaginal disc growth Source: FlyBase
  • positive regulation of Notch signaling pathway Source: FlyBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
  • regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  • regulation of proteolysis Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • visual perception Source: UniProtKB-KW
  • wing disc development Source: FlyBase

Keywordsi

Biological processCell cycle, Sensory transduction, Ubl conjugation pathway, Vision

Enzyme and pathway databases

ReactomeiR-DME-3214815 HDACs deacetylate histones
SignaLinkiQ95RJ9

Names & Taxonomyi

Protein namesi
Recommended name:
F-box-like/WD repeat-containing protein ebi
Gene namesi
Name:ebi
ORF Names:CG4063
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0263933 ebi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16L → Q in ebiCC1; induces ectopic S phases within the peripheral and central nervous system. 1
Mutagenesisi510C → Y in ebi4; induces defects in R7 cell development in the eye. 1
Mutagenesisi602S → L in ebiCC3; induces ectopic S phases within the peripheral and central nervous system. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509601 – 700F-box-like/WD repeat-containing protein ebiAdd BLAST700

Proteomic databases

PaxDbiQ95RJ9
PRIDEiQ95RJ9

Expressioni

Tissue specificityi

Widely expressed both in embryos and larvae.1 Publication

Gene expression databases

BgeeiFBgn0263933
ExpressionAtlasiQ95RJ9 differential
GenevisibleiQ95RJ9 DM

Interactioni

Subunit structurei

Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • repressing transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi59468, 66 interactors
IntActiQ95RJ9, 7 interactors
MINTiQ95RJ9
STRINGi7227.FBpp0077715

Structurei

3D structure databases

ProteinModelPortaliQ95RJ9
SMRiQ95RJ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 36LisHPROSITE-ProRule annotationAdd BLAST33
Domaini41 – 84F-box-likeAdd BLAST44
Repeati353 – 392WD 1Add BLAST40
Repeati408 – 447WD 2Add BLAST40
Repeati449 – 488WD 3Add BLAST40
Repeati491 – 531WD 4Add BLAST41
Repeati532 – 571WD 5Add BLAST40
Repeati574 – 622WD 6Add BLAST49
Repeati625 – 664WD 7Add BLAST40
Repeati666 – 700WD 8Add BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi206 – 323Ser-richAdd BLAST118

Domaini

The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.

Sequence similaritiesi

Belongs to the WD repeat EBI family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0273 Eukaryota
ENOG410XRSY LUCA
GeneTreeiENSGT00890000139430
InParanoidiQ95RJ9
KOiK04508
OMAiLNYLIWR
OrthoDBiEOG091G04MW
PhylomeDBiQ95RJ9

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR006594 LisH
IPR011047 Quinoprotein_ADH-like_supfam
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
PfamiView protein in Pfam
PF08513 LisH, 1 hit
PF00400 WD40, 6 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00667 LisH, 1 hit
SM00320 WD40, 8 hits
SUPFAMiSSF50998 SSF50998, 1 hit
PROSITEiView protein in PROSITE
PS50896 LISH, 1 hit
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q95RJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSSDEVNF LVYRYLQESG FLHSAYVFGI ESHISQSNIN GALVPPAALL
60 70 80 90 100
TILQKGLLYT EVEWSVGEDG EVARPIEGLS LIDAVMPEVK PLKPIVKTEP
110 120 130 140 150
GKPGAVDSSA PAGGNQNNNA KPEIKIEPGT GVAGSAGGNK IAGSTTGTST
160 170 180 190 200
PTDQSASEVD SSGNAANNAG GTYAGNNGAG GNQASTGGSN STSTPAGGDL
210 220 230 240 250
AAPGASQKKS QNSNEAGSSS SGNAGNANAT STDDAASSTS TNGNSSTSSS
260 270 280 290 300
VEQPTSGLTP AGGTVSTSNP DAAASGGAST ATGSKAPSGA VTIRVGAQGN
310 320 330 340 350
NVQSGSSNAQ SSAPSGTISS STSGGAGTPA ALVPMDIDEN IEIPESKARV
360 370 380 390 400
LRGHESEVFI CAWNPSRDLL ASGSGDSTAR IWDMSDANTN SNQLVLRHCI
410 420 430 440 450
QKGGAEVPSN KDVTSLDWNC DGSLLATGSY DGYARIWKTD GRLASTLGQH
460 470 480 490 500
KGPIFALKWN KCGNYILSAG VDKTTIIWDA STGQCTQQFA FHSAPALDVD
510 520 530 540 550
WQTNQAFASC STDQRIHVCR LGVNEPIKTF KGHTNEVNAI KWCPQGQLLA
560 570 580 590 600
SCSDDMTLKI WSMNRDRCCH DLQAHSKEIY TIKWSPTGPG TNNPNTNLIL
610 620 630 640 650
ASASFDSTVR LWDVERGSCI HTLTKHTEPV YSVAFSPDGK HLASGSFDKC
660 670 680 690 700
VHIWSTQTGQ LVHSYKGTGG IFEVCWNSKG TKVGASASDG SVFVLDLRKF
Length:700
Mass (Da):72,387
Last modified:April 26, 2004 - v2
Checksum:i28C6D8D07BB79FB7
GO

Sequence cautioni

The sequence AAL28874 differs from that shown. Reason: Frameshift at position 484.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146345 mRNA Translation: AAD35017.1
AE014134 Genomic DNA Translation: AAF51501.1
AY061326 mRNA Translation: AAL28874.1 Frameshift.
RefSeqiNP_477329.1, NM_057981.4
UniGeneiDm.3639

Genome annotation databases

EnsemblMetazoaiFBtr0078055; FBpp0077715; FBgn0263933
GeneIDi33212
KEGGidme:Dmel_CG4063

Similar proteinsi

Entry informationi

Entry nameiEBI_DROME
AccessioniPrimary (citable) accession number: Q95RJ9
Secondary accession number(s): Q9XZK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health