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Protein

F-box-like/WD repeat-containing protein ebi

Gene

ebi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

F-box-like component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, cone cell development and neuronal cell cycle control. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Required to block the S phase entry in the peripheral nervous system and central nervous system in a process that does not involve the degradation of ttk. Involved in cone cell development by preventing the transcriptional repression mediated by Su(H) on Dl, probably by participating in a E3 complex that contains sno and mediates the ubiquitination and subsequent proteasomal degradation of some component of the Su(H) repressor complex.3 Publications

GO - Molecular functioni

  • chromatin binding Source: FlyBase
  • GTP binding Source: FlyBase
  • repressing transcription factor binding Source: FlyBase

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to light stimulus Source: FlyBase
  • chaeta development Source: FlyBase
  • compound eye cone cell fate commitment Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • negative regulation of JNK cascade Source: FlyBase
  • negative regulation of neuron apoptotic process Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: GOC
  • neuron cellular homeostasis Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • photoreceptor cell development Source: FlyBase
  • positive regulation of histone deacetylation Source: FlyBase
  • positive regulation of imaginal disc growth Source: FlyBase
  • positive regulation of Notch signaling pathway Source: FlyBase
  • regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  • regulation of proteolysis Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • visual perception Source: UniProtKB-KW
  • wing disc development Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Sensory transduction, Ubl conjugation pathway, Vision

Enzyme and pathway databases

SignaLinkiQ95RJ9.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box-like/WD repeat-containing protein ebi
Gene namesi
Name:ebi
ORF Names:CG4063
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0263933. ebi.

Subcellular locationi

GO - Cellular componenti

  • histone deacetylase complex Source: GO_Central
  • nucleus Source: UniProtKB
  • polytene chromosome Source: FlyBase
  • transcriptional repressor complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16L → Q in ebiCC1; induces ectopic S phases within the peripheral and central nervous system. 1
Mutagenesisi510C → Y in ebi4; induces defects in R7 cell development in the eye. 1
Mutagenesisi602S → L in ebiCC3; induces ectopic S phases within the peripheral and central nervous system. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509601 – 700F-box-like/WD repeat-containing protein ebiAdd BLAST700

Proteomic databases

PaxDbiQ95RJ9.
PRIDEiQ95RJ9.

Expressioni

Tissue specificityi

Widely expressed both in embryos and larvae.1 Publication

Gene expression databases

BgeeiFBgn0263933.
GenevisibleiQ95RJ9. DM.

Interactioni

Subunit structurei

Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC3Q9VNC22EBI-421390,EBI-128090
phylQ279344EBI-421390,EBI-77033
sinaP214613EBI-421390,EBI-77019
snaP080442EBI-421390,EBI-152305
ttkP422822EBI-421390,EBI-77008

GO - Molecular functioni

  • repressing transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi59468. 52 interactors.
IntActiQ95RJ9. 7 interactors.
MINTiMINT-6540401.
STRINGi7227.FBpp0077715.

Structurei

3D structure databases

ProteinModelPortaliQ95RJ9.
SMRiQ95RJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 36LisHPROSITE-ProRule annotationAdd BLAST33
Domaini41 – 84F-box-likeAdd BLAST44
Repeati353 – 392WD 1Add BLAST40
Repeati408 – 447WD 2Add BLAST40
Repeati449 – 488WD 3Add BLAST40
Repeati491 – 531WD 4Add BLAST41
Repeati532 – 571WD 5Add BLAST40
Repeati574 – 622WD 6Add BLAST49
Repeati625 – 664WD 7Add BLAST40
Repeati666 – 700WD 8Add BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi206 – 323Ser-richAdd BLAST118

Domaini

The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.

Sequence similaritiesi

Belongs to the WD repeat EBI family.Curated
Contains 1 F-box-like domain.Curated
Contains 1 LisH domain.PROSITE-ProRule annotation
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0273. Eukaryota.
ENOG410XRSY. LUCA.
GeneTreeiENSGT00860000133748.
InParanoidiQ95RJ9.
KOiK04508.
OMAiLANNHSD.
OrthoDBiEOG091G04MW.
PhylomeDBiQ95RJ9.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q95RJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSSDEVNF LVYRYLQESG FLHSAYVFGI ESHISQSNIN GALVPPAALL
60 70 80 90 100
TILQKGLLYT EVEWSVGEDG EVARPIEGLS LIDAVMPEVK PLKPIVKTEP
110 120 130 140 150
GKPGAVDSSA PAGGNQNNNA KPEIKIEPGT GVAGSAGGNK IAGSTTGTST
160 170 180 190 200
PTDQSASEVD SSGNAANNAG GTYAGNNGAG GNQASTGGSN STSTPAGGDL
210 220 230 240 250
AAPGASQKKS QNSNEAGSSS SGNAGNANAT STDDAASSTS TNGNSSTSSS
260 270 280 290 300
VEQPTSGLTP AGGTVSTSNP DAAASGGAST ATGSKAPSGA VTIRVGAQGN
310 320 330 340 350
NVQSGSSNAQ SSAPSGTISS STSGGAGTPA ALVPMDIDEN IEIPESKARV
360 370 380 390 400
LRGHESEVFI CAWNPSRDLL ASGSGDSTAR IWDMSDANTN SNQLVLRHCI
410 420 430 440 450
QKGGAEVPSN KDVTSLDWNC DGSLLATGSY DGYARIWKTD GRLASTLGQH
460 470 480 490 500
KGPIFALKWN KCGNYILSAG VDKTTIIWDA STGQCTQQFA FHSAPALDVD
510 520 530 540 550
WQTNQAFASC STDQRIHVCR LGVNEPIKTF KGHTNEVNAI KWCPQGQLLA
560 570 580 590 600
SCSDDMTLKI WSMNRDRCCH DLQAHSKEIY TIKWSPTGPG TNNPNTNLIL
610 620 630 640 650
ASASFDSTVR LWDVERGSCI HTLTKHTEPV YSVAFSPDGK HLASGSFDKC
660 670 680 690 700
VHIWSTQTGQ LVHSYKGTGG IFEVCWNSKG TKVGASASDG SVFVLDLRKF
Length:700
Mass (Da):72,387
Last modified:April 26, 2004 - v2
Checksum:i28C6D8D07BB79FB7
GO

Sequence cautioni

The sequence AAL28874 differs from that shown. Reason: Frameshift at position 484.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146345 mRNA. Translation: AAD35017.1.
AE014134 Genomic DNA. Translation: AAF51501.1.
AY061326 mRNA. Translation: AAL28874.1. Frameshift.
RefSeqiNP_477329.1. NM_057981.4.
UniGeneiDm.3639.

Genome annotation databases

EnsemblMetazoaiFBtr0078055; FBpp0077715; FBgn0263933.
GeneIDi33212.
KEGGidme:Dmel_CG4063.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146345 mRNA. Translation: AAD35017.1.
AE014134 Genomic DNA. Translation: AAF51501.1.
AY061326 mRNA. Translation: AAL28874.1. Frameshift.
RefSeqiNP_477329.1. NM_057981.4.
UniGeneiDm.3639.

3D structure databases

ProteinModelPortaliQ95RJ9.
SMRiQ95RJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59468. 52 interactors.
IntActiQ95RJ9. 7 interactors.
MINTiMINT-6540401.
STRINGi7227.FBpp0077715.

Proteomic databases

PaxDbiQ95RJ9.
PRIDEiQ95RJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078055; FBpp0077715; FBgn0263933.
GeneIDi33212.
KEGGidme:Dmel_CG4063.

Organism-specific databases

CTDi33212.
FlyBaseiFBgn0263933. ebi.

Phylogenomic databases

eggNOGiKOG0273. Eukaryota.
ENOG410XRSY. LUCA.
GeneTreeiENSGT00860000133748.
InParanoidiQ95RJ9.
KOiK04508.
OMAiLANNHSD.
OrthoDBiEOG091G04MW.
PhylomeDBiQ95RJ9.

Enzyme and pathway databases

SignaLinkiQ95RJ9.

Miscellaneous databases

GenomeRNAii33212.
PROiQ95RJ9.

Gene expression databases

BgeeiFBgn0263933.
GenevisibleiQ95RJ9. DM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEBI_DROME
AccessioniPrimary (citable) accession number: Q95RJ9
Secondary accession number(s): Q9XZK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

'Ebi' means 'shrimp' in Japanese.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.