Reviewed, UniProtKB/Swiss-Prot Q95QJ7 (GPR1_CAEEL)

Names and origin

Protein names

Recommended name:
    G-protein regulator 1

Gene names

Name:	gpr-1
ORF Names:	F22B7.13

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complex of gpr-1 and gpr-2, in association with lin-5, activates G-protein signaling to affect mitotic spindle force. Polarity determinants (par genes) may regulate lin-5/gpr-1/gpr-2/goa-1 locally to create the asymmetric forces that drive spindle movement. Ref.3
Subunit structure	Interacts with gpr-1, lin-5 and GDP-bound goa-1. Ref.3
Subcellular location	Cytoplasm › cell cortex. Spindle. Note: Located to the spindle and cell cortex when in complex with lin-5 and gpr-1. Ref.3
Sequence similarities	Contains 1 GoLoco domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytokinesis Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase meiotic chromosome segregation Inferred from mutant phenotype. Source: WormBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle organization Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
atn-1	Q23158	1	EBI-316069,EBI-314014
goa-1	P51875	1	EBI-316069,EBI-316062
gpa-16	Q9N2V6	1	EBI-316069,EBI-1005005

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	G-protein regulator 1		PRO_0000087562
Regions
	Domain	424 – 445	22	GoLoco

Sequences

	Sequence		Length	Mass (Da)
	Q95QJ7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: EC68F930E3622AB5	FASTA	525	60,128
	10 20 30 40 50 60 MDVSYYDGPK DEVAEAMLKS AVTAMRLGQY EDGKGRLEEI MEFGTSNFQL LGTIYMYYGR 70 80 90 100 110 120 VCRHLNHDAK ALEFFEHELN MFKLIFNYPE ACDSTRRIVE QALKMGKFPK ARRFAEDLID 130 140 150 160 170 180 YTSNKKNGEK YIGQARILFA SVCLEGCERD VESNQDEKKK LLSICAEQIA AVKLFNENNT 190 200 210 220 230 240 EGAVSETKIM LLEAKCLSLD EKYEESRRKY QECIDFAIKT DQFEAVHIAY YDKALYAETD 250 260 270 280 290 300 LLFFIIRDLR SALFYATKFG KERDVVKYKS KLSEEMLRNG EFHEAYLYGL EALVSIRKLG 310 320 330 340 350 360 LNEYIGDVLL TIAKCLIALG KRRQAAYFII LGSVLTINQN SFKLFYEQID VAMNQERSET 370 380 390 400 410 420 ATDQDVCLAI DSSPDPTSSN DMINKFVVEL EHATNVETWE MIVNGIIDDQ KKPVAIEKKE 430 440 450 460 470 480 NEEPVDMMDL IFSMSSRMDD QRTELPAARF IPPRPVSSAS KKTTKSHRIL PGLRANWTKV 490 500 510 520 QSMKFDGHTM NRILKRSKKS KSSLDSTNSM QGDDTRSDDV TMTSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"A complex of LIN-5 and GPR proteins regulates G protein signaling and spindle function in C elegans." Srinivasan D.G., Fisk R.M., Xu H., van den Heuvel S. Genes Dev. 17:1225-1239(2003) [PubMed: 12730122] [Abstract] Cited for: FUNCTION, INTERACTION WITH GPR-1; LIN-5 AND GOA-1, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12018 Genomic DNA. Translation: AAK68341.1.
PIR	S44636.
RefSeq	NP_498900.1.
UniGene	Cel.8150
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26900N.
IntAct	Q95QJ7. 12 interactions.
Genome annotation databases
Ensembl	F22B7.13. Caenorhabditis elegans. [Contig view]
GeneID	176210.
KEGG	cel:F22B7.13.
NMPDR	fig|6239.3.peg.10902.
Organism-specific databases
WormBase	WBGene00001688. gpr-1.
WormPep	F22B7.13. CE24910. [WorfDB]
Phylogenomic databases
OMA	Q95QJ7. ARSAMKL.
Gene expression databases
ArrayExpress	Q95QJ7.
Family and domain databases
InterPro	IPR003109. GoLoco_motif. IPR015609. Hsp40/DnaJ_Rel. [Graphical view]
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF02188. GoLoco. 1 hit. [Graphical view]
SMART	SM00390. GoLoco. 1 hit. [Graphical view]
PROSITE	PS50877. GOLOCO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891594.

Entry information

Entry name

GPR1_CAEEL

Accession

Primary (citable) accession number: Q95QJ7
Secondary accession number(s): P34408

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project