Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Target of rapamycin homolog

Gene

let-363

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that regulates the mRNA translation machinery, probably by modulating the activity of translation factors such as eIF-4G and eIF-2. It may have some protein kinase activity instead of lipid kinase activity (PubMed:12225660). Required, during larval development, for the establishment of the proper number of germline progenitors, probably upstream of rsks-1 and ife-1 (PubMed:22278922). Required for larval development (PubMed:22278922). May act as a mediator of lifespan regulation by insulin signaling and nutrient sensing (PubMed:14668850).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

GO - Molecular functioni

GO - Biological processi

  • box C/D snoRNP assembly Source: WormBase
  • determination of adult lifespan Source: UniProtKB
  • DNA repair Source: GO_Central
  • mesendoderm development Source: WormBase
  • negative regulation of gene expression Source: UniProtKB
  • nematode larval development Source: WormBase
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of translation Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of translation Source: WormBase
  • response to sodium arsenite Source: UniProtKB
  • TOR signaling Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ95Q95.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin homolog (EC:2.7.11.1)
Alternative name(s):
CeTOR
Lethal protein 363
Gene namesi
Name:let-363
Synonyms:tor
ORF Names:B0261.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0261.2a; CE32559; WBGene00002583; let-363.
B0261.2b; CE32560; WBGene00002583; let-363.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • nucleus Source: GO_Central
  • TORC1 complex Source: WormBase
  • TORC2 complex Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects strongly increase lifespan, the mean lifetime being of 25 days instead of 10 days, suggesting that it may be involved in aging process (PubMed:14668850). RNAi-mediated knockdown in the germline causes sterility, a severe reduction in the number of germline progenitors and a delay in G2 phase of the cell cycle (PubMed:22278922). RNAi-mediated knockdown enhances the loss of touch receptor neurons in a mec-4 u231 mutant (PubMed:17327275).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26972697Target of rapamycin homologPRO_0000088811Add
BLAST

Proteomic databases

PaxDbiQ95Q95.
PeptideAtlasiQ95Q95.
PRIDEiQ95Q95.

PTM databases

iPTMnetiQ95Q95.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in all major tissues and organs, including the intestine, gonads and hypodermal cells (PubMed:12225660). Expressed in neurons (PubMed:17327275).2 Publications

Gene expression databases

BgeeiWBGene00002583.

Interactioni

Protein-protein interaction databases

BioGridi37625. 1 interaction.
STRINGi6239.B0261.2a.2.

Structurei

3D structure databases

ProteinModelPortaliQ95Q95.
SMRiQ95Q95. Positions 1443-1936, 2189-2287, 2322-2565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati235 – 27238HEAT 1Add
BLAST
Repeati649 – 68739HEAT 2Add
BLAST
Repeati689 – 72638HEAT 3Add
BLAST
Repeati731 – 76838HEAT 4Add
BLAST
Repeati815 – 85339HEAT 5Add
BLAST
Repeati863 – 90038HEAT 6Add
BLAST
Repeati1073 – 111038HEAT 7Add
BLAST
Domaini1438 – 2153716FATPROSITE-ProRule annotationAdd
BLAST
Domaini2358 – 2664307PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST
Domaini2665 – 269733FATCPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1941 – 202383Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.Curated
Contains 1 FAT domain.PROSITE-ProRule annotation
Contains 1 FATC domain.PROSITE-ProRule annotation
Contains 7 HEAT repeats.Curated
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0891. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000163215.
InParanoidiQ95Q95.
KOiK07203.
OrthoDBiEOG091G0046.
PhylomeDBiQ95Q95.

Family and domain databases

Gene3Di1.10.1070.11. 3 hits.
1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024585. DUF3385_TOR.
IPR003152. FATC_dom.
IPR009076. FRB_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR026683. TOR.
[Graphical view]
PANTHERiPTHR11139:SF9. PTHR11139:SF9. 7 hits.
PfamiPF11865. DUF3385. 1 hit.
PF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08771. Rapamycin_bind. 1 hit.
[Graphical view]
SMARTiSM01346. DUF3385. 1 hit.
SM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF47212. SSF47212. 1 hit.
SSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q95Q95-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQQHGISFQ MNADRQNKAA TTSNRIVSAP ATNEFDERHR KQGIDAARAL
60 70 80 90 100
ASQYRSRITE NRDANQRQAA RELSRYVRSE LKDEPNTFSD AFLNAIDGRT
110 120 130 140 150
DIASQSAIYN CMKSNSNIDQ KRAGIYLIVC LAETHSGNVI RYANYLLKML
160 170 180 190 200
NNGNGLDEDT VKMASKALAF LIATCKSYAA ELVDRCLDHC HEWLGQNVPH
210 220 230 240 250
SQQPKNQQEI DQIRRLAASH LSRELALATP TAFFLRVNLF FKYIFNAVRD
260 270 280 290 300
KNPAVRIAGI DALHVVLTIV SQREAKNKTE WFKKCFDEAL EGQPNPSQKD
310 320 330 340 350
DLDRWHAVAL ILNELLRISD QRFELIRCES SQFIKQKFLK EDEEEGVEWL
360 370 380 390 400
VLTKQQTIVE SVTARKLVLE RFPKILDCVR QIIPLANKTS STKQQSSIYL
410 420 430 440 450
NTVLMQLLPR ICAFPQCDRT FQTISFDTSF TILQRNAVAA PAIGMMMLSN
460 470 480 490 500
PDVHATHIEK TISFISAAIK KTTNSDVLDN YFTFLFLFVD AYHEQVTTQI
510 520 530 540 550
KAIIPQLMDI TLSRSLANVL KMIMMRIPKL RLNVQDGVMA SVYQTLTGSL
560 570 580 590 600
IPPKSEPIGR PASPKAILQK AETDPKELQR IVLAVDVLGE FYFSRGALQR
610 620 630 640 650
IMQYVADYYL TADNVEIRLA AVSSCCEMVV PFVGVYKKVT SDKRNSLLQT
660 670 680 690 700
IYGVLRAVCS VIVNDQDVRV RMQVISCFGQ MPRPFLAHLA QPEMLEVQFM
710 720 730 740 750
ALHDEKLEMQ QACVTLLGRL AELNPALVLP RLRLMLLETL SQMQQSGQAR
760 770 780 790 800
LEQHSAKMIA QLAKQSPKFM RPYVGSLMIA MIPKLRNDQK YAEVTAQVLN
810 820 830 840 850
AVSEIAVIGG AEIVKNLKPL FEKLTHMIND SSSLHKREAA LRAIGGICRS
860 870 880 890 900
TAYVVDPYRD YPSLLDDLLR ILKTVMSNTM RREAIKTLGI LGAIDPYTHK
910 920 930 940 950
VFTGSVQSST AISTALSLPI SETDSKDPRQ DIIHWFNYEK CTLEEFYPAI
960 970 980 990 1000
TIANLMLMMQ DEDSQSYAEI AQAIVTIFRS LGDMAPLYTE QVIPRLIEVC
1010 1020 1030 1040 1050
RRATESSNRA NLREFFLQQL ANFVAIIRKH AAPYMPAIFT IIADAWKEDI
1060 1070 1080 1090 1100
SVKMVVIEVL TDMGTAIGND FSKYTGELIP YLLTVLQTDK TKERVLTVKV
1110 1120 1130 1140 1150
MESIQKLTHC IVQHLHLVLP PLLIILDDFS LKLSIRNTAL STVLHMTQQV
1160 1170 1180 1190 1200
DVSAYAPRMM QSWHHNISTA EMRDKLLLLL IEIIKQLGKF FDIFKRGVDQ
1210 1220 1230 1240 1250
KLRDYNLDKS VHYEQYRKLA QRAQMSRDVL TSSVFAGSNG NIQYSSTQAG
1260 1270 1280 1290 1300
MRGQANNVYA NNDLHERLMN GSIDSGASRQ DNRDDYYRYG VEEKKEVPKV
1310 1320 1330 1340 1350
APTTARPTSE LVTVQITKQR LNKDALMPQW KNENLTSKDE WLQWLMKIRI
1360 1370 1380 1390 1400
GFLTYGSSPS LRAASSLGDQ HPHLARDLFP AAFMSVWTEL DSDVQNDLTS
1410 1420 1430 1440 1450
CLLRAISTGI PELIQTILNL AEFMDHSEKG PLPISHDVLG RWAEQTKAFA
1460 1470 1480 1490 1500
KACRYKEMSV LKKSGSMQTT FTRKVKLEPN DCQSLITYAN KLNVQEEAAG
1510 1520 1530 1540 1550
VVRYAERNEM NFQMRGRWYE KLNEWEKALG AYELEEKKKS SCPNLQVYDE
1560 1570 1580 1590 1600
KDHLMTPEEA ATAEEARMHE MRCLEALGRW DELNSKSVVW ADQRGNRNDS
1610 1620 1630 1640 1650
VRDEINKKQL DHKMAVIAAR GAWAVDNWER MADYVSVISE NTQDGAMLRA
1660 1670 1680 1690 1700
VVAVHNDENT KAMGLIEKVR EMIDSELTAM ANESYERAYI PMVSVQQMAE
1710 1720 1730 1740 1750
LEEAIEYKTR PERRPRIALL WSRRLQGCRR NVEQWQRLIM LRGLVLSPQE
1760 1770 1780 1790 1800
MHPLRVKFSS MCRKQGKNSM SRAVLRELLS LPANSDLVRA KAPFDKPLLV
1810 1820 1830 1840 1850
LALAKQLYQD DHKDEAIRAL EDLANHWNKR INPIPKATGR ELIPPSTKEP
1860 1870 1880 1890 1900
ARICAKVLLK LGEWTELKSK TSNNMQVGEL SFVRQQVSPQ YRTKESRTPE
1910 1920 1930 1940 1950
TIAFENTINY YQQATQYDPG WHKVWHKLAS THFYAVCRER PHPTTVISPP
1960 1970 1980 1990 2000
QQPQQPKKMH IPPVTRATSP PPPAQKSPQP APFHSITEPL SVIIDYPVPP
2010 2020 2030 2040 2050
PLGSLVGLPP MPAYLSSNSS LPPQHHHVSP LSNDSPSNSA ENKLYLKHAA
2060 2070 2080 2090 2100
HAVRCFAKAL MCSPGSRLED TLRLMQLWFD HGDDKDQDVY FALTETIFDL
2110 2120 2130 2140 2150
PVTTWLEAIP QLMARLDCPD DQKSVQLVLR VLCEIARHRP QAVIYALTVA
2160 2170 2180 2190 2200
SRSKDVHRSK NAGTVLEKMM EYHSKLVREA SLVTEELVRC AILWHEQWHD
2210 2220 2230 2240 2250
ALDDASRVYF HRRLQDNNVQ AMFDALRNMN DLMQKGAPTT MKEHSFQQTY
2260 2270 2280 2290 2300
SSDLKEAGRY VQAFESSGNV KDLNQAWEIY CSVFKKLRDQ LATLNSLDLV
2310 2320 2330 2340 2350
YVSPNLVSAK DLELVVPGTY DPSAPIVSIQ SFSSKMNVIT SKQRPRKMVI
2360 2370 2380 2390 2400
RGSNGLDYQF LLKGHEDPRQ DERVMQLFGL VNTLLANNSE TCRRNLTIQR
2410 2420 2430 2440 2450
YSIVALSKDS GLIGWVPNCD TLHTLVKEYR EKKAKIPLSI EHKTLQKLSL
2460 2470 2480 2490 2500
ETEHLTLMQK LQLFESALSV TQGEDLRHVL WLKSPSSEVW FDRRTNYTRS
2510 2520 2530 2540 2550
VACMSMVGYI LGLGDRHPSN LMLDRLTGKV VHIDFGDCFE VAMLREKFPE
2560 2570 2580 2590 2600
RVPFRLTRML INAMEVTGLD GVYNYTAERV LKMLRTNQES LLAVLEAFVY
2610 2620 2630 2640 2650
DPVINWRLVE GMKKDPKTRK DTGGRQNMAG AVLPSSSTTD SIMETIKRKL
2660 2670 2680 2690
DGTEFVHTDG STPPEPLQVT EQLAMLTEQA TSPLNLCQSY IGWCPFW
Length:2,697
Mass (Da):306,529
Last modified:January 16, 2004 - v3
Checksum:iCBC59D6CE02BFEC0
GO
Isoform b (identifier: Q95Q95-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2212-2215: Missing.

Show »
Length:2,693
Mass (Da):305,975
Checksum:i7AE68EB029A26C0B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1295 – 12951K → ISE in CCD61569 (PubMed:9851916).Curated
Sequence conflicti1295 – 12951K → ISE in CCD61570 (PubMed:9851916).Curated
Sequence conflicti1992 – 19943Missing in CCD61570 (PubMed:9851916).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2212 – 22154Missing in isoform b. CuratedVSP_009223

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080142 Genomic DNA. Translation: CCD61569.1.
FO080142 Genomic DNA. Translation: CCD61570.1.
RefSeqiNP_491549.2. NM_059148.4.
NP_491552.2. NM_059151.5.
UniGeneiCel.19302.

Genome annotation databases

GeneIDi172167.
KEGGicel:CELE_B0261.2.
UCSCiB0261.2b. c. elegans. [Q95Q95-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080142 Genomic DNA. Translation: CCD61569.1.
FO080142 Genomic DNA. Translation: CCD61570.1.
RefSeqiNP_491549.2. NM_059148.4.
NP_491552.2. NM_059151.5.
UniGeneiCel.19302.

3D structure databases

ProteinModelPortaliQ95Q95.
SMRiQ95Q95. Positions 1443-1936, 2189-2287, 2322-2565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37625. 1 interaction.
STRINGi6239.B0261.2a.2.

PTM databases

iPTMnetiQ95Q95.

Proteomic databases

PaxDbiQ95Q95.
PeptideAtlasiQ95Q95.
PRIDEiQ95Q95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi172167.
KEGGicel:CELE_B0261.2.
UCSCiB0261.2b. c. elegans. [Q95Q95-1]

Organism-specific databases

CTDi172167.
WormBaseiB0261.2a; CE32559; WBGene00002583; let-363.
B0261.2b; CE32560; WBGene00002583; let-363.

Phylogenomic databases

eggNOGiKOG0891. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000163215.
InParanoidiQ95Q95.
KOiK07203.
OrthoDBiEOG091G0046.
PhylomeDBiQ95Q95.

Enzyme and pathway databases

SignaLinkiQ95Q95.

Miscellaneous databases

PROiQ95Q95.

Gene expression databases

BgeeiWBGene00002583.

Family and domain databases

Gene3Di1.10.1070.11. 3 hits.
1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024585. DUF3385_TOR.
IPR003152. FATC_dom.
IPR009076. FRB_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR026683. TOR.
[Graphical view]
PANTHERiPTHR11139:SF9. PTHR11139:SF9. 7 hits.
PfamiPF11865. DUF3385. 1 hit.
PF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08771. Rapamycin_bind. 1 hit.
[Graphical view]
SMARTiSM01346. DUF3385. 1 hit.
SM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF47212. SSF47212. 1 hit.
SSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOR_CAEEL
AccessioniPrimary (citable) accession number: Q95Q95
Secondary accession number(s): O01438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: September 7, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.