Reviewed, UniProtKB/Swiss-Prot Q95NN1 (T23O_TRICA)

Names and origin

Protein names	Recommended name:     Tryptophan 2,3-dioxygenase       Short name=TDO     EC=1.13.11.11 Alternative name(s):     Tryptophan pyrrolase       Short name=Tryptophanase     Tryptophan oxygenase       Short name=TRPO       Short name=TO     Tryptamin 2,3-dioxygenase
Organism	Tribolium castaneum (Red flour beetle)
Taxonomic identifier	7070 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Tenebrionidae › Tribolium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity. Required for normal eye pigmentation.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Domain	Coiled coil
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Tryptophan 2,3-dioxygenase		PRO_0000360886
Regions
	Region	24 – 28	5	Substrate binding By similarity
	Region	54 – 58	5	Substrate binding By similarity
	Coiled coil	205 – 235	31	Potential
Sites
	Metal binding	309	1	Iron (heme axial ligand) By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	131	1	Heme By similarity
	Binding site	324	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95NN1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 96A34C73ED0F1F03	FASTA	388	45,423
	10 20 30 40 50 60 MSCPLRPSEA QEGDQLSEEC GMLYGEYLML DKILEAQRLL SEQNNQPVHD EHLFIVTHQA 70 80 90 100 110 120 YELWFKQIIY ELDSIRNIFS DVLEESQTLE ILKRLNRVVL ILKVLVDQVM ILETMTPLDF 130 140 150 160 170 180 MDFRCYLRPA SGFQSLQFRL LENKLGVRQE NRVKYNQNYS KVFGNDEKAL EQIAKSEKEP 190 200 210 220 230 240 SLTDLVQRWL ERTPGLELEG FNFWGKYQKA VEKLLTEQKE LAEKEEAETL KRYKLNDLEK 250 260 270 280 290 300 RREVYESIFK VEVHEALMSR GERRFSHKAL QGAIMITFYR DEPRFSQPHQ ILTLLMDIDS 310 320 330 340 350 360 LITKWRYNHV LMVQRMIGSS QLGTGGSSGY QYLRSTLSDR YKVFVDLFNL STFLIPRSYI 370 380 PPLSTSMRSH LCNWGSANST NIVSNGNN

References

[1]

"Cloning and characterization of the Tribolium castaneum eye-color genes encoding tryptophan oxygenase and kynurenine 3-monooxygenase." Lorenzen M.D., Brown S.J., Denell R.E., Beeman R.W. Genetics 160:225-234(2002) [PubMed: 11805058] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: GA-1 and GA-2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY052390 mRNA. Translation: AAL15464.1. AY052392 Genomic DNA. Translation: AAL15466.1.
RefSeq	NP_001034499.1.
UniGene	Tca.5753
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	654844.
KEGG	tca:654844.
Organism-specific databases
CTD	654844.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_TRICA

Accession

Primary (citable) accession number: Q95NN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	December 1, 2001
Last modified:	September 1, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)