Reviewed, UniProtKB/Swiss-Prot Q95M30 (HCK_MACFA)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase HCK
    EC=2.7.10.2
Alternative name(s):
    Hemopoietic cell kinase
    p56-HCK

Gene names

Name:

HCK

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 504	503	Tyrosine-protein kinase HCK		PRO_0000088103
Regions
	Domain	56 – 116	61	SH3
	Domain	122 – 219	98	SH2
	Domain	240 – 493	254	Protein kinase
	Nucleotide binding	246 – 254	9	ATP By similarity
Sites
	Active site	359	1	Proton acceptor By similarity
	Binding site	268	1	ATP By similarity
Amino acid modifications
	Modified residue	15	1	Phosphothreonine By similarity
	Modified residue	30	1	Phosphotyrosine By similarity
	Modified residue	105	1	Phosphotyrosine By similarity
	Modified residue	180	1	Phosphothreonine By similarity
	Modified residue	187	1	Phosphotyrosine By similarity
	Modified residue	308	1	Phosphotyrosine By similarity
	Modified residue	389	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	440	1	Phosphoserine By similarity
	Modified residue	493	1	Phosphotyrosine By similarity
	Modified residue	498	1	Phosphoserine By similarity
	Modified residue	500	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q95M30-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 53B29322D2DE3423	FASTA	504	57,096
	10 20 30 40 50 60 MGCMKSKFLQ AGGNTFSKTE TSANPHCPVY VPDPTSTIKP GPNSNNRNTP GIGEGSEDII 70 80 90 100 110 120 VVALYDYEAI HHEDLSFQKG DQMVVLEESG EWWKARSLAT RKEGYIPSNY VARVDSLETE 130 140 150 160 170 180 EWFFKGISRK DAERQLLAPG NMLGSFMIRD SETTKGSYSL SVRDYDPRQG DTVKHYKIRT 190 200 210 220 230 240 LDNGGFYISP RSTFSTLQEL VDHYKKGSDG LCQKLSVPCV SSKPQKPWEK DAWEIPRESL 250 260 270 280 290 300 KLEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF LAEANLMKTL QHDKLVKLHA 310 320 330 340 350 360 VVTKEPIYII TEFMAKGSLL DFLKSDEGSK QPLPKLIDFS AQIAEGMAFI EQRNYIHRDL 370 380 390 400 410 420 RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK WTAPEAINFG SSTIKSDVWS 430 440 450 460 470 480 FGILLMEIVT YGRIPYPGMS NPEVIRALER GYRMPRPENC PEELYNIMMR CWKNRPEERP 490 500 TFEYIQSVLD DFYTATESQY QQQP

References

[1]	Picard C. Thesis (2001), University of Marseille, France Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ320181 mRNA. Translation: CAC44031.1.
3D structure databases
SMR	Q95M30. Positions 56-116, 59-504.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q95M30.
Enzyme and pathway databases
BRENDA	2.7.10.2. 3438.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR020748. Tyr_kinase_non-rcpt_Hck. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256:SF259. Tyr_kinase_non-rcpt_Hck. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCK_MACFA

Accession

Primary (citable) accession number: Q95M30

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)